Publication Information

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'https://openalex.org/W1997735348', 'https://openalex.org/W2000870025', 'https://openalex.org/W2035930721', 'https://openalex.org/W2043403724', 'https://openalex.org/W2046171723', 'https://openalex.org/W2056007355', 'https://openalex.org/W2058582010', 'https://openalex.org/W2060519231', 'https://openalex.org/W2083576326', 'https://openalex.org/W2096471353', 'https://openalex.org/W2100837269', 'https://openalex.org/W2169809507', 'https://openalex.org/W2327112872', 'https://openalex.org/W2413764872', 'https://openalex.org/W2464321911', 'https://openalex.org/W2987032079'], 'related_works': ['https://openalex.org/W4387497383', 'https://openalex.org/W2948807893', 'https://openalex.org/W2778153218', 'https://openalex.org/W2748952813', 'https://openalex.org/W2527526854', 'https://openalex.org/W2463320161', 'https://openalex.org/W2374614804', 'https://openalex.org/W2078814861', 'https://openalex.org/W1976181487', 'https://openalex.org/W1531601525'], 'abstract_inverted_index': {'フッ素による培養細胞の増殖抑制機構を検討するため,': [0], 'HeLa細胞ならびにフッ素耐性HeLa細胞': [1], '(FR細胞)': [2], 'を用いて実験を行った。耐性細胞は最高2mMのNaFを添加した培地でHeLa細胞を継代し樹立したものである。その結果次の成績が得られた。1.': [3], 'HeLa細胞の増殖は,': [4], '0.5mMNaFでわずかに抑制され,': [5], '2mMNaFでは全く阻止された。FR細胞は,': [6], '2mM': [7, 17], 'NaFでも対照と同じ増殖を示した。2.': [8], '2mMNaF存在下でHeLa細胞のタンパク質合成は強く阻害されたが,': [9], 'FR細胞でのタンパク質合成阻害はわずかであった。3.': [10], 'HeLa細胞とFR細胞の細胞質のタンパク質組成をSDS-ポリアクリルアミドゲル電気泳動': [11], '(SDS-PAGE)': [12], 'で調べたところ,': [13], 'クーマシーブルー染色で,': [14], '両細胞間のペプチドバンドのパターンに明らかな違いは認められなかった。4.': [15], 'HeLa細胞とFR細胞を,': [16], 'NaF処理した後合成されたタンパク質をSDS-PAGE,': [18], 'オートラジオグラフィーで調べたところ,': [19], 'HeLa細胞では,': [20], '分子量約77000ダルトンの強くラベルされる特異的なペプチドが認められた。FR細胞では,': [21], 'NaFの作用をうけないHeLa細胞と同様なペプチドバンドのパターンが見られた。5.': [22], 'この特異的な77000ダルトンのペプチドは,': [23], 'フッ素処理時間の増加と共に強くラベルされる傾向を示した。6.': [24], '77000ダルトンのペプチドの細胞内局在は,': [25], '形質膜,': [26], 'ミトコンドリア分画,': [27], 'ミクロゾーム分画に認められたが,': [28], '核,': [29], '可溶性分画には認められなかった。': [30]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2319590788', 'counts_by_year': [], 'updated_date': '2024-12-08T23:13:48.119895', 'created_date': '2016-06-24'}