Title: STUDIES ON O(H) BLOOD GROUP SUBSTANCES OF HUMAN ERYTHROCYTES BY BLOOD GROUP SPECIFIC ENZYMES
Abstract:1. Preparation of O(H) -decomposing enzyme (Fr. 14-18) from Bac. fulminans, abolishes the O(H) - specific activity of human erythrocytes, stroma, and glycolipids belonging to the blood group OLe (a-b+...1. Preparation of O(H) -decomposing enzyme (Fr. 14-18) from Bac. fulminans, abolishes the O(H) - specific activity of human erythrocytes, stroma, and glycolipids belonging to the blood group OLe (a-b+), and of human erythrocytes of blood group OLe(a+b-), liberating from them only fucose as a monosaccharide.2. Preparation of O(H) -decomposing enzyme from Bac. cereus, abolishes the O(H) -specific activity of human erythrocyes, stroma and glycolipids belonging to the blood group OLe(a-b+), and of human erythrocytes of blood group OLe(a+b-), liberating from them fucose and galactose as monosaccharides.Furthermore, Leb-specific activity of the blood group OLe(a-b+) erythrocytes was abolished by the action of this enzyme.3. O(H) -combining sites on an erythrocyte of blood group OLe(a-b+) are estimated to 8.5×106, and those on an erythrocyte of blood group OLe(a+b-) 77.5 ×106.Read More