Title: Inhibition of nitric oxide production and proinflammatory cytokines by several medicinal plants.
Abstract: Event Abstract Back to Event Inhibition of nitric oxide production and proinflammatory cytokines by several medicinal plants. Fathollah Kalantar1*, Saeid M. Hosseini1, Hoeesin Gholmoghadam1, Mehdi Shabani1 and Zahra Amirghofran1 1 Shiraz University of medical sciences, Immunology, Iran BACKGROUND: A number of medicinal plants have been used to treat various immunological diseases. Nitric oxide (NO) is involved in regulation of immune responses. OBJECTIVE: This study investigates whether some extracts of medicinal plants modulate the production of NO. Plants are native to Iran including: Dracocephalum kotschyi, Linum persicum, Dionysia termeana, Salvia mirzayanii, Ferulago angulata and Euphorbia cheiradenia Methods: The methanolic extracts of the plants were prepared and checked for their effects on the NO production by lipopolysaccharide-stimulated mouse macrophages. IL-1 and TNF-alpha pro-inflammatory cytokines production in the macrophage culture were measured using enzyme-linked immunosorbent. RESULTS: After 24 hours treatment the cells significant decrease (p<0.001) in NO production for all extracts at concentration of 50 μg/ml were observed. This inhibitory effect was also seen after 48 hours. L. persicum was the most powerful extract in reducing the NO production at 1 µg/ml after both 24 and 48-hours (nearly 100% inhibition, p<0.001) in comparison to the other extracts. Among the extracts, S. mirzayanii extract with 66.2 ± 8% inhibition at 50 µg/ml, showed the mildest effects in 48 hour culture. Determination of cytokine release, the extract of L. persicum significantly decreased both TNF-α and IL-1β cytokines production by stimulated macrophages (p<0.001). D. kotschyi, D. termeana and F. angulata inhibited secretion of IL-1β from the cells. CONCLUSION: According to the results, presences of anti-inflammatory and macrophage inhibitory substances exist in these extracts and these extracts could be an alternative for the treatment of a wide range inflammatory disease. Keywords: Cytokines, medicinal plants, Nitric Oxide, inflammatory disease, Macrophages Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Innate immunity Citation: Kalantar F, Hosseini SM, Gholmoghadam H, Shabani M and Amirghofran Z (2013). Inhibition of nitric oxide production and proinflammatory cytokines by several medicinal plants.. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.01048 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 30 Jun 2013; Published Online: 22 Aug 2013. * Correspondence: Mr. Fathollah Kalantar, Shiraz University of medical sciences, Immunology, Shiraz, Fars, 7134845794, Iran, [email protected] Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Fathollah Kalantar Saeid M Hosseini Hoeesin Gholmoghadam Mehdi Shabani Zahra Amirghofran Google Fathollah Kalantar Saeid M Hosseini Hoeesin Gholmoghadam Mehdi Shabani Zahra Amirghofran Google Scholar Fathollah Kalantar Saeid M Hosseini Hoeesin Gholmoghadam Mehdi Shabani Zahra Amirghofran PubMed Fathollah Kalantar Saeid M Hosseini Hoeesin Gholmoghadam Mehdi Shabani Zahra Amirghofran Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.