Title: Recombinant P. aeruginosa outer membrane proteins and immune response
Abstract: Event Abstract Back to Event Recombinant P. aeruginosa outer membrane proteins and immune response Olga V. Lebedinskaya1*, Alena V. Soldatenkova2, Lidia A. Geiderova2, Elvin A. Akhmatov2, Natalia A. Mikhailova2 and Nelly K. Akhmatova2 1 Acad. E.A. Wagner Perm State Medical Academy, Russia 2 I.I. Mechnikov Research Institute for Vaccines and Sera, RAMS, Russia There is an urgent need to develop new vaccines for prevention and immunotherapy of infections caused by P. aeruginosa since the agent has a high level of antimicrobial resistance, and the clinical course of disease is characterized by multiple organ damage. Induction of immune response under the influence of P. aeruginosa antigens is still poorly characterized. To study the cytokine-mediated immune response in mice immunized with recombinant antigens of P. aeruginosa. Recombinant P. aeruginosa outer membrane proteins OprL, OprF, hybrid protein OprF-I, which consists of of OprF and OprI sequences and recombinant toxoid (aTox) were produced. To obtain the expression of these proteins the BL21(DE3) and M15 Escherichia coli were used. Mice were injected intraperitoneally with 50 mg of P. aeruginosa antigenic preparations. Cytokine levels were determined in mice serum with test system FlowCytomixMouse Thl/Th2 10 plex. We found that injection of antigenic preparations a wide spectrum of cytokines in mice was induced. The highest levels were observed in the ratio of IL-1 and IL-6 when oprF-I, oprL, aTox, oprF antigens were administered. OprFI actively stimulated the production of IL-2, and IL-5, IL-10, TNF-□ and IFN-□. OprF-I antigen contributed to the induction of IL-6, IL-17, TNF-a and IFN□, while aTox – to the expression of IL-1, IL-2, IFN-□. OprL antigen induced IL-17 and TNF-alpha□to greatest extent, while IL-10 – to lesser extent. The antigens of P. aeruginosa intraperitoneally administered to mice favored the formation of the immune response to induce both Th-1 and Th-2 pathways. Keywords: P. aeruginosa, recombinant antigens, Mice, cytokine-mediated immune response, Immunotherapy Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Host-pathogen interactions Citation: Lebedinskaya OV, Soldatenkova AV, Geiderova LA, Akhmatov EA, Mikhailova NA and Akhmatova NK (2013). Recombinant P. aeruginosa outer membrane proteins and immune response. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00848 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 19 Jun 2013; Published Online: 22 Aug 2013. * Correspondence: Ms. Olga V Lebedinskaya, Acad. E.A. Wagner Perm State Medical Academy, Perm, Russia, [email protected] Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Olga V Lebedinskaya Alena V Soldatenkova Lidia A Geiderova Elvin A Akhmatov Natalia A Mikhailova Nelly K Akhmatova Google Olga V Lebedinskaya Alena V Soldatenkova Lidia A Geiderova Elvin A Akhmatov Natalia A Mikhailova Nelly K Akhmatova Google Scholar Olga V Lebedinskaya Alena V Soldatenkova Lidia A Geiderova Elvin A Akhmatov Natalia A Mikhailova Nelly K Akhmatova PubMed Olga V Lebedinskaya Alena V Soldatenkova Lidia A Geiderova Elvin A Akhmatov Natalia A Mikhailova Nelly K Akhmatova Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.