Title: "Macrophages in a state of flux": The role of CD68-positive macrophages in the biocompatibility of biomaterials
Abstract: Event Abstract Back to Event "Macrophages in a state of flux": The role of CD68-positive macrophages in the biocompatibility of biomaterials Christoph Brochhausen1, 2, Volker H. Schmitt1, Andreas Mamilos1, 2, Christine Schmitt1, Constanze N. Planck1, Taufiek K. Rajab3, Helmut Hierlemann4 and C James Kirkpatrick1 1 University Medical Centre, REPAIR-lab, Institute of Pathology, Germany 2 University Hospital, REPAIR-lab, Institute of Pathology, Germany 3 Havard Medical School, Brigham and Women's Hospital, United States 4 Innovations GmbH, Polymedics, Germany Introduction: In the regulation of inflammation and biocompatibility macrophages are crucially involved. Today it is well accepted that macrophages represent a dynamic cell type with several functional properties. In the present study we analysed the amount of CD68-positive macrophages in comparison with the extent of fibrosis and inflammation in a standardized adhesion-prevention animal model after use of 5 different adhesion barriers and an untreated control group. Materials and Methods: Serosal wounding was performed by a standardised method in female Wistar rats. The lesions remained untreated (control, n=14) or were treated with different clinically available barrier materials (Adept®, n=14; Intercoat®, n=33; Spraygel®, n=8; Seprafilm®, n= 29; SupraSeal®, n=14. After 14 days the animals were sacrificed, the treated tissue was explanted and processed by standardised techniques for histological evaluation of fibrosis using Ladewig staining, haematoxylin & eosin and chloracetate esterase to estimate inflammation as well as immunohistology using a monoclonal antibody against CD68 to score macrophages, this antigen being common to all functional states of this cell type. Histology was evaluated by two independent investigators. Results: No correlation was found between the number of CD68-positive macrophages and the amount of fibrosis or the extent of inflammation. While some groups were associated with moderate infiltration of macrophages and no fibrosis (Intercoat® and SupraSeal®), the Seprafilm® group revealed moderate inflammation with likewise moderate numbers of macrophages. On the other hand, a minimal number of macrophages was associated with minimal fibrosis in the control and Adept® group. Inflammation and macrophage infiltration were moderate in the SupraSeal® group. In the Intercoat® and Seprafilm® treated animals moderate infiltration of macrophages was seen with minimal inflammation. Animals from the control and Adept® group revealed minimal inflammation and minimal macrophage infiltration. All in all, there was absolutely no correlation between the tissue response and the count of CD 68-positive macrophages. Conclusion: The results revealed an interesting discrepancy between the different functional properties of macrophages, the cellular tissue response to anti-adhesive biomaterials and the CD68-positive macrophages. Since it is known that subtypes of macrophages have pro- or anti-adhesive as well as pro-fibrotic properties, we conclude that for biocompatibility studies the immunohistological subclassification of macrophages should be performed to delineate the specific effects of the used biomaterial on the cellular tissue reaction. These data should also be considered with respect to biocompatibility testing directives as recommended in the ISO-norm. Keywords: Biocompatibility, biodegredation Conference: 10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016. Presentation Type: Poster Topic: Biomaterials evaluation in animal models Citation: Brochhausen C, Schmitt VH, Mamilos A, Schmitt C, Planck CN, Rajab TK, Hierlemann H and Kirkpatrick C (2016). "Macrophages in a state of flux": The role of CD68-positive macrophages in the biocompatibility of biomaterials. Front. Bioeng. Biotechnol. Conference Abstract: 10th World Biomaterials Congress. doi: 10.3389/conf.FBIOE.2016.01.02932 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Mar 2016; Published Online: 30 Mar 2016. Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Christoph Brochhausen Volker H Schmitt Andreas Mamilos Christine Schmitt Constanze N Planck Taufiek K Rajab Helmut Hierlemann C James Kirkpatrick Google Christoph Brochhausen Volker H Schmitt Andreas Mamilos Christine Schmitt Constanze N Planck Taufiek K Rajab Helmut Hierlemann C James Kirkpatrick Google Scholar Christoph Brochhausen Volker H Schmitt Andreas Mamilos Christine Schmitt Constanze N Planck Taufiek K Rajab Helmut Hierlemann C James Kirkpatrick PubMed Christoph Brochhausen Volker H Schmitt Andreas Mamilos Christine Schmitt Constanze N Planck Taufiek K Rajab Helmut Hierlemann C James Kirkpatrick Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.