Title: Development of a colloidal gold immunochromatographic assay for antigen detection of wild-type classical swine fever virus.
Abstract:A colloidal gold immunochromatographic strip was developed for the rapid detection of the wild-type classical swine fever virus(CSFV) antigens using two MAb against the E2 protein.The wild-type CSFV-s...A colloidal gold immunochromatographic strip was developed for the rapid detection of the wild-type classical swine fever virus(CSFV) antigens using two MAb against the E2 protein.The wild-type CSFV-specific 6E10 MAb was labeled with colloidal gold as capture antibody and the HQ06 MAb was immobilized on the test line as detection antibody,while a rabbit anti-mouse IgG antibody was blotted on the control line of the nitrocellulose membrane.The strips were tested on CSFV infected PK-15 cell cultures and normal cells.The results showed that specific signal developed in the test line when detecting the CSFV-infected cells,but not with the non-infected cells.The detection limit of the strip was 103.5 TCID50 CSFV.The test strips did not react with hog cholera lapinized virus,bovine viral diarrhea virus porcine reproductive and respiratory syndrome virus,transmissible gastroenteritis virus,porcine epidemic diarrhea virus,porcine rotavirus,pseudorabies virus,porcine parvovirus,and porcine circovirus type 2.Taken together,the test strip was specific,sensitive and reproducible,which could be used to differentiate cells infected with wild-type CSFV from those infected with HCLV.Read More
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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Cited By Count: 1
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