Title: Virus-free propagation technology for Yulin garlic using shoot tip culture.
Abstract: The present study has been conducted to develop a virus-free propagation technology for growing Yulin garlic.Shoot tips of Yulin garlic plants with 1-3 leaf primordia were used as explants and cultured using MS or B5 basic medium with different hormone concentrations(1.0-2.0 mg/L 6-BA,0.1-0.5 mg/L NAA).The results showed that the optimal medium for the induction of adventitious buds was B5+1.5mg/L 6-BA+0.5mg/L NAA,which recorded 96% ad-ventitious buds induction rate.The optimal medium for bud multiplication was B5+0.5mg/L 6-BA + 0.05mg/L NAA,where the multiplication multiple reached 4.5 times.The ELISA results showed that the virus-free rate of shoot tip was over 90%,when shoot tip was 0.5-0.6mm and with 1-2 leaf primordia.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot