Structural insights into the substrate specificity of two esterases from the thermophilic Rhizomucor miehei

Name: Work Video:
Duration: 3 min 30 s
Shaoqing Yang; Zhen Qin; Xiaojie Duan; Qiaojuan Yan; Zhengqiang Jiang
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'to': [23, 112, 217, 306, 492, 497, 592, 705, 922, 1129, 1396, 1650, 1664, 1688, 1718, 1731, 1755, 1937, 1944, 1957, 2109, 2867, 2924, 3105, 3137, 3150, 3164, 3180, 3269, 3745, 3776, 3944, 3988, 4031], 'show': [24, 218], 'great': [25, 219], 'potential': [26, 220], 'in': [27, 81, 88, 92, 125, 150, 221, 275, 282, 286, 319, 344, 481, 576, 597, 613, 882, 895, 1070, 1118, 1388, 1467, 1520, 1644, 1758, 2027, 2138, 2188, 2220, 2255, 2270, 2331, 2528, 2540, 2693, 2792, 2846, 2851, 2871, 2952, 2990, 3004, 3036, 3062, 3092, 3121, 3490, 3502, 3509, 3518, 3528, 3537, 3540, 3613, 3617, 3626, 3635, 3644, 3647, 3656, 3683, 3690, 3695, 3701, 3723, 3731, 3766, 3772, 3805, 3809, 3926, 4034, 4052, 4183, 4197], 'industrial': [28, 190, 222, 384, 598, 691], 'applications.': [29, 223], 'In': [30, 224, 3028], 'this': [31, 135, 225, 329, 1533, 3678, 3989, 4063, 4074], 'study,': [32, 226], 'crystal': [34, 228, 1701, 2240, 2574, 2944, 2963], 'structures': [35, 51, 173, 229, 245, 367, 1413, 1702, 1983, 2945, 2964, 3189, 3440, 3470], 'of': [36, 52, 75, 99, 128, 132, 169, 186, 192, 230, 246, 269, 293, 322, 326, 363, 380, 386, 395, 405, 421, 433, 436, 461, 515, 571, 595, 634, 695, 730, 761, 796, 811, 835, 855, 880, 889, 914, 919, 925, 941, 953, 981, 993, 996, 1065, 1082, 1113, 1122, 1147, 1176, 1188, 1191, 1221, 1232, 1257, 1285, 1344, 1363, 1414, 1434, 1445, 1491, 1498, 1574, 1585, 1610, 1639, 1660, 1684, 1693, 1703, 1776, 1787, 1812, 1840, 1901, 1929, 1984, 2020, 2030, 2045, 2072, 2098, 2106, 2165, 2169, 2204, 2279, 2326, 2345, 2358, 2371, 2381, 2443, 2468, 2524, 2569, 2598, 2606, 2612, 2735, 2748, 2768, 2811, 2824, 2835, 2861, 2913, 2921, 2928, 2946, 2965, 2997, 3014, 3043, 3065, 3085, 3124, 3130, 3139, 3143, 3152, 3157, 3166, 3174, 3182, 3190, 3238, 3244, 3248, 3261, 3295, 3301, 3313, 3360, 3364, 3417, 3421, 3441, 3450, 3454, 3471, 3479, 3499, 3556, 3585, 3623, 3632, 3640, 3652, 3663, 3671, 3708, 3739, 3762, 3803, 3824, 3832, 3850, 3862, 3872, 3877, 3885, 3908, 3911, 3915, 3935, 3975, 4022, 4038, 4055, 4059, 4070, 4087, 4095, 4102, 4115, 4146, 4169, 4176, 4190], 'RmEstA': [37, 53, 100, 231, 247, 294, 1557, 1640, 1673, 2170, 2196, 2280, 2372, 2947, 2966, 2998, 3015, 3032, 3176, 3191, 3315, 3322, 3442, 3529, 3538, 3645, 3657, 3691, 3806, 3818, 3825, 3851, 3873, 3912, 3976, 4177, 4191], 'RmEstB': [39, 55, 89, 102, 233, 249, 283, 296, 1559, 1642, 1681, 1841, 2205, 2228, 2282, 2346, 2382, 2862, 2949, 2968, 3086, 3184, 3193, 3317, 3379, 3444, 3732, 3820, 3827, 3853, 3878, 3916, 3936, 4179, 4193, 4230, 4239], 'were': [40, 90, 234, 284, 1749, 1842, 1861, 1873, 1881, 1942, 2144, 2171, 2198, 2206, 2230, 2283, 2312, 2390, 2463, 2485, 2526, 2559, 2590, 2626, 2770, 2837, 2864, 2950, 2969, 3021, 3099, 3119, 3133, 3264, 3487, 3658, 3924, 4081, 4090], 'determined': [41, 235, 1963, 2348, 2970], 'at': [42, 236, 1539, 1727, 2059, 2093, 2152, 2273, 2288, 2291, 2301, 2878, 2971, 3292, 3553, 3667], '2.15': [43, 237], 'Å': [44, 47, 238, 241, 2973, 2976, 3612, 3616, 3834, 3939], '2.43': [46, 240], 'resolutions,': [48, 242, 2977], 'respectively.': [49, 243, 2308, 2843, 2961, 2978, 3378, 3435, 3676], 'The': [50, 117, 177, 244, 311, 371, 949, 1379, 1431, 1457, 1530, 1636, 1738, 1879, 1927, 2055, 2064, 2083, 2195, 2227, 2343, 2369, 2387, 2482, 2633, 2937, 2962, 2979, 2993, 3081, 3096, 3171, 3188, 3231, 3258, 3276, 3299, 3484, 3533, 3649, 3685, 3712, 3735, 3847, 3932, 3972, 4015, 4137], 'showed': [56, 250, 3461, 4140], 'two': [57, 251, 1415, 1546, 1705, 1739, 3002, 3042, 3048, 3066, 3073, 3102, 3198, 3895, 4024, 4076, 4088], 'distinctive': [58, 252], 'domains,': [59, 253], 'a': [60, 64, 254, 258, 392, 422, 470, 516, 735, 982, 1053, 1068, 1114, 1145, 1177, 1222, 1258, 1349, 1356, 1364, 1375, 1440, 1446, 1455, 1463, 1495, 1575, 1611, 1715, 1724, 1777, 1813, 1902, 2174, 2209, 2248, 2256, 2410, 2847, 3034, 3108, 3200, 3216, 3245, 3280, 3560, 3661, 3855, 3978], 'catalytic': [61, 140, 154, 255, 334, 348, 708, 726, 1400, 1432, 1441, 1515, 3201, 3232, 3496, 3946], 'domain': [62, 256, 3202, 3218], 'cap': [65, 259, 3217, 3882, 3896], 'domain,': [66, 260, 3858], 'with': [67, 261, 765, 1017, 1052, 1125, 1267, 1398, 1418, 1620, 1822, 1844, 1911, 2014, 2161, 2173, 2208, 2263, 2489, 2561, 2628, 3001, 3038, 3047, 3068, 3089, 3284, 3306, 3318, 3327, 3356, 3384, 3413, 3448, 4093, 4119, 4229, 4238], 'classical': [69, 263, 3495], 'α/β-hydrolase': [70, 264, 916, 1358, 3240, 3456], 'fold.': [71, 265], 'Catalytic': [72, 266], 'triads': [73, 267], 'consisting': [74, 268, 3243, 3498], 'residues': [76, 270, 3012, 3071, 3209, 3225, 3693, 3718, 3875], 'Ser161,': [77, 271], 'Asp262,': [78, 272], 'His292': [80, 274, 3507, 3633, 3653], 'RmEstA,': [82, 276, 3208, 3224, 3614, 3724, 4170], 'Ser164,': [84, 278], 'Asp261,': [85, 279], 'His291': [87, 281], 'found': [91, 285, 1649, 3061, 3488, 3543, 3682, 3706], 'respective': [94, 288, 3887], 'canonical': [95, 289, 3103, 3236], 'positions.': [96, 290], 'Structural': [97, 291, 2522, 2552], 'comparison': [98, 292], 'revealed': [103, 297, 3475, 3813], 'that': [104, 298, 472, 957, 3055, 3181, 3437, 3893, 3918, 4094, 4159], 'their': [105, 113, 299, 307, 493, 707, 856, 1524, 1694, 1709, 1735, 3886, 3919], 'specificity': [108, 302, 2860, 3771, 4069], 'might': [109, 303, 4029, 4061], 'be': [110, 304, 1651, 3195], 'attributed': [111, 305], 'different': [114, 308, 1419, 1695, 2881], 'substrate-binding': [115, 130, 309, 324, 3928, 3933, 3973, 4020, 4057], 'pockets.': [116, 310], 'aromatic': [118, 312, 4045], 'amino': [119, 313, 883, 1380, 4046], 'acids': [120, 314, 464, 4047], 'Phe222': [121, 315], 'Trp92,': [123, 317], 'located': [124, 318, 1461, 3552, 3666, 4051], 'center': [127, 321, 4054], 'pocket': [131, 325, 3934, 3974, 4058, 4064], 'RmEstB,': [133, 327, 4060, 4171], 'blocked': [134, 328], 'pocket,': [136, 330], 'thus': [137, 331], 'narrowing': [138, 332], 'its': [139, 333, 642, 3593], 'range': [141, 335, 2704, 2757], 'for': [142, 188, 336, 382, 523, 1532, 2040, 2061, 2414, 2460, 2507, 2744, 2772, 2820, 2839, 2943, 2982, 3207, 3213, 3223, 3229, 3835], 'substrates': [143, 158, 337, 352, 1638, 4041], '(C2–C8).': [144, 338], 'mutants': [146, 340, 2863, 4077, 4089], '(F222A': [147, 341], 'W92F': [149, 343], 'showing': [152, 346, 1229, 1582, 1784], 'higher': [153, 347], 'activity': [155, 349, 455, 770, 1056, 1676, 1961, 2100, 2135, 2915, 4101, 4114], 'toward': [156, 350, 456, 802, 1677, 4106, 4117, 4148, 4160], 'long-chain': [157, 351], 'further': [159, 189, 353, 383, 3106], 'confirmed': [160, 354], 'hypothesized': [162, 356], 'interference.': [163, 357], 'This': [164, 358, 1712, 3949], 'is': [165, 359, 703, 939, 1361, 1437, 1460, 1517, 1714, 2731, 2739, 2807, 2815, 3551, 3698, 3891], 'first': [167, 361, 1223, 1576, 1716, 1778], 'report': [168, 362, 1699], 'HSL': [170, 364, 950, 1019, 1045, 1148, 1345, 1352, 1548, 1720, 3481, 3703, 3778], 'esterase': [172, 366, 428, 518, 636, 670, 732, 764, 798, 836, 988, 1116, 1183, 1225, 1263, 1347, 1578, 1616, 1780, 1818, 1907, 3587], 'filamentous': [175, 369, 1350, 1725], 'fungi.jlr': [176, 370], 'information': [178, 372], 'on': [179, 373, 699, 877, 1057, 1368, 1439, 3024, 3468, 3794], 'structure-function': [180, 374], 'relationships': [181, 375, 1081], 'could': [182, 376, 3194], 'open': [183, 377], 'important': [184, 378], 'avenues': [185, 379], 'exploration': [187, 381], 'applications': [191, 385], 'esterases.': [193, 387, 3321, 3483], 'Esterases': [388], '(EC': [389], '3.1.1.1)': [390], 'are': [391, 478, 588, 958, 1527, 2691, 2794, 2853, 2988], 'general': [393], 'class': [394], 'carboxylic': [396, 463], 'ester': [397, 406, 805, 1233, 1586, 1788, 4040], 'hydrolases,': [398, 1523], 'which': [399, 1360, 3550, 3697], 'catalyze': [400], 'cleavage': [402], 'formation': [404, 3123], 'bonds': [407, 3118, 3665], '(1.Wei': [408, 968, 1163], 'T.': [409, 902, 969, 1023, 1079, 1164, 1474], 'Feng': [410, 829, 970, 1165], 'S.': [411, 787, 789, 826, 830, 971, 1166, 1214, 1247, 1567, 1600, 1769, 1802, 1891], 'Mao': [412, 972, 1167], 'D.': [413, 666, 973, 1100, 1168], 'Yu': [414, 974, 1169], 'X.': [415, 419, 565, 975, 979, 1170, 1174, 1216, 1249, 1569, 1602, 1771, 1804, 1893], 'Du': [416, 976, 1171], 'C.': [417, 722, 977, 1021, 1172, 1478, 2604], 'Wang': [418, 978, 1173], 'Characterization': [420, 514, 980, 1175, 1256, 1609, 1811, 1900], 'new': [423, 983, 1178, 2848], 'acid': [426, 884, 986, 1181, 1381, 1450, 3011], 'tolerant': [427, 987, 1182], 'Thermotoga': [430, 990, 1185], 'maritima': [431, 991, 1186], 'capable': [432, 992, 1187], 'hydrolytic': [434, 994, 1189], 'resolution': [435, 995, 1190, 2798, 2857], 'racemic': [437, 997, 1192], 'ketoprofen': [438, 998, 1193], 'ethyl': [439, 804, 999, 1194], 'ester.J.': [440, 1000, 1195], 'Mol.': [441, 1001, 1196, 1272, 1625, 1827, 1916, 3780], 'Catal.,': [442, 1002, 1197, 1273, 1626, 1828, 1917], 'B': [443, 1003, 1198, 1274, 1627, 1829, 1918], 'Enzym.': [444, 1004, 1199, 1275, 1628, 1830, 1919], '2013;': [445, 929, 1005, 1200, 1236, 1589, 1791], '85–86:': [446, 1006, 1201], '23-30Crossref': [447, 1007, 1202], 'Scopus': [448, 533, 556, 582, 653, 685, 744, 780, 821, 849, 933, 1008, 1041, 1091, 1203, 1240, 1279, 1427, 1509, 1593, 1632, 1795, 1834, 1923, 2003, 2339, 2426, 2453, 2518, 2548, 2584, 2621, 2682, 3604, 3786], '(23)': [449, 850, 1009, 1204], 'Google': [450, 535, 558, 584, 655, 687, 746, 782, 823, 851, 935, 1010, 1043, 1093, 1141, 1205, 1242, 1281, 1429, 1511, 1595, 1634, 1797, 1836, 1925, 2005, 2341, 2428, 2455, 2520, 2550, 2586, 2623, 2684, 3606, 3788], 'Scholar).': [451, 585, 688, 852, 936, 1044, 1142, 1282, 1430, 1512, 1635, 1837, 1926, 2342, 2521, 2551, 2587, 2624, 2643, 2685, 3607], 'They': [452, 477], 'exhibit': [453, 1048, 1669], 'maximum': [454], 'water-soluble': [457], 'or': [458, 1073, 1452, 3807], 'emulsified': [459], 'esters': [460, 2883, 4118], 'short-chain': [462, 1652], '(less': [465], 'than': [466], '10': [467, 2062], 'carbon': [468, 4035], 'atoms),': [469], 'property': [471], 'distinguishes': [473], 'them': [474, 1066, 1286], 'lipases.': [476], 'widely': [479], 'used': [480, 2771, 2838], 'food,': [483], 'perfume,': [484], 'cosmetic,': [485], 'chemical,': [486], 'agricultural,': [487], 'pharmaceutical': [489], 'industries,': [490], 'owing': [491], 'unique': [494], 'properties:': [495], 'tolerance': [496], 'organic': [498, 614], 'solvents,': [499], 'stereoselectivity': [503], '(2.Esteban-Torres': [504], 'M.': [505, 538, 1098, 1407, 3754, 3760], 'Mancheno': [506], 'J.M.': [507], 'de': [508], 'las': [509], 'Rivas': [510], 'B.': [511, 660, 2435], 'Munoz': [512], 'R.': [513, 1106, 1746, 2657, 2669], 'cold-active': [517], 'Lactobacillus': [520], 'plantarum': [521], 'suitable': [522], 'food': [524], 'fermentations.J.': [525], 'Agric.': [526], 'Food': [527], 'Chem.': [528, 1133], '2014;': [529, 740, 845, 1276, 1423, 1629, 1831, 1920, 1999], '62:': [530], '5126-5132Crossref': [531], 'PubMed': [532, 555, 581, 652, 743, 779, 848, 932, 1040, 1090, 1140, 1239, 1426, 1508, 1592, 1794, 2002, 2338, 2425, 2452, 2517, 2547, 2583, 2620, 2681, 3603, 3785], '(32)': [534], 'Scholar,': [536, 559, 656, 747, 783, 824, 1094, 1206, 1243, 1596, 1798], '3.Nardini': [537], 'Dijkstra': [539, 1410], 'B.W.': [540, 1411], 'Alpha/beta': [541], 'hydrolase': [542, 1436], 'fold': [543, 917, 3241, 3457], 'enzymes:': [544], 'keeps': [547], 'growing.Curr.': [548], 'Opin.': [549], 'Struct.': [550, 647, 1503, 1997, 3598], 'Biol.': [551, 648, 1132, 1504, 1998, 2420, 2447, 2512, 2578, 3599, 3781], '1999;': [552, 1505, 2580], '9:': [553], '732-737Crossref': [554], '(678)': [557], '4.Chen': [560], 'Q.': [561, 1212, 1245, 1565, 1598, 1767, 1800, 1889], 'Luan': [562], 'Z.J.': [563], 'Cheng': [564], 'Xu': [566, 756, 1209, 1250, 1562, 1603, 1764, 1805, 1894], 'J.H.': [567, 757], 'Molecular': [568, 1028, 1111, 2635], 'dynamics': [569], 'investigation': [570], 'binding': [574], 'mechanism': [575, 1433, 1516, 1993], 'carboxylesterase.Biochemistry.': [577], '2015;': [578, 776], '54:': [579], '1841-1848Crossref': [580], '(27)': [583], 'However,': [586, 1337, 3677, 4111], 'there': [587, 1338], 'still': [589], 'some': [590], 'barriers': [591], 'use': [594], 'applications,': [599, 692], 'such': [600, 4123], 'as': [601, 893, 1374, 1885, 1964, 2011, 2103, 2131, 2365, 2383, 2884, 2918, 3101, 3504, 3511, 3520, 4124], 'low': [602, 3462], 'production': [603], 'yield,': [604], 'limited': [605], 'pH': [606, 1949, 2035, 2077, 2193, 2225, 2876], 'thermal': [608, 728], 'stability,': [609], 'poor': [611], 'performance': [612], 'solvents': [615], '(5.Byun': [616, 3567], 'J.S.': [617, 3568], 'Rhee': [618, 3569], 'J.K.': [619, 3570], 'Kim': [620, 624, 717, 786, 3571, 3575], 'N.D.': [621, 3572], 'Yoon': [622, 788, 3573], 'J.': [623, 664, 716, 718, 785, 1085, 1971, 1977, 3574], 'D.U.': [625, 3576], 'Koh': [626, 3577], 'E.': [627, 791, 904, 3578], 'Oh': [628, 3579], 'J.W.': [629, 3580], 'Cho': [630, 3581], 'H.S.': [631, 3582], 'Crystal': [632, 1412, 1489, 1982, 3583], 'structure': [633, 857, 1490, 2344, 2357, 2370, 2380, 2416, 2505, 2986, 3173, 3584], 'hyperthermophilic': [635, 3586], 'EstE1': [637, 3588], 'relationship': [640, 3591], 'between': [641, 2571, 3592, 3619, 3817, 4018], 'dimerization': [643, 3594], 'thermostability': [645, 714, 834, 3596], 'properties.BMC': [646, 3597], '2007;': [649, 2678, 3600], '7:': [650, 3601], '47Crossref': [651, 3602], '(70)': [654, 3605, 3787], '6.Kumar': [657], 'L.': [658, 2533, 3750, 3752, 3756], 'Singh': [659], 'Adhikari': [661], 'D.K.': [662], 'Mukherjee': [663], 'Ghosh': [665], 'A': [667, 1493, 3132, 3145, 3159, 3494, 3608], 'thermoalkaliphilic': [668], 'halotolerant': [669], 'Rhodococcus': [672], 'sp.': [673], 'LKE-028': [674], '(MTCC': [675], '5562):': [676], 'enzyme': [677, 2023, 2099, 2107, 2869, 2914, 2922, 3767], 'purification': [678], 'characterization.Process': [680], 'Biochem.': [681, 1086], '2012;': [682], '47:': [683], '983-991Crossref': [684], '(24)': [686], 'For': [689, 1855, 1867, 2235], 'certain': [690], 'functional': [693], 'modification': [694], 'enzymes': [697, 865, 1397, 1662], 'based': [698, 1438], 'rational': [700, 862], 'protein': [701, 1377, 1940, 2159, 2573, 2607, 3242, 3942, 3984], 'design': [702], 'necessary': [704], 'enhance': [706], 'efficiency,': [709], 'enantioselectivity,': [712], '(7.Boyineni': [715], 'Kang': [719], 'B.S.': [720], 'Lee': [721], 'Jang': [723], 'S.H.': [724], 'Enhanced': [725], 'site': [727], 'stability': [729, 3769], 'cold-adapted': [731], 'EstK': [733], 'by': [734, 806, 840, 1371, 1934, 1966, 2068, 2089, 2125, 2146, 2155, 2349, 2375, 2465, 2478, 2592, 2648, 3254, 3266, 3660, 3717, 3726, 3957, 3993, 4130, 4153], 'W208Y': [736], 'mutation.Biochim.': [737], 'Biophys.': [738, 1421], 'Acta.': [739, 1422], '1844:': [741, 1424], '1076-1082Crossref': [742], '(14)': [745, 1280, 1633, 1835, 1924], '8.Liu': [748], 'J.Y.': [749], 'Bian': [750], 'H.P.': [751], 'Tang': [752], 'Y.': [753, 793, 906, 1208, 1253, 1472, 1561, 1606, 1763, 1808, 1897], 'Bai': [754], 'Y.P.': [755], 'Double': [758], 'substi\xadtuted': [759], 'variant': [760], 'Bacillus': [762, 1322, 1416], 'amyloliquefaciens': [763], 'enhanced': [766], 'enantioselectivity': [767, 795], 'high': [769, 1014, 1126, 1230, 1583, 1785, 3310, 3324, 3381, 3446, 3814], 'towards': [771], '1-(3′,4′-methylenedioxyphenyl)ethyl': [772], 'acetate.Appl.': [773], 'Microbiol.': [774, 814], 'Biotechnol.': [775], '99:': [777], '1701-1708Crossref': [778], '(9)': [781], '9.Kim': [784], 'Hong': [790], 'Ryu': [792], 'Improved': [794, 833], 'thermostable': [797], 'Archaeoglobus': [800, 1304], 'fulgidus': [801, 1305, 2363, 3338, 3395], '(S)-ketoprofen': [803], 'directed': [807], 'evolution': [808, 1393], 'characterization': [810, 1220, 1573, 1775], 'mutant': [812, 4104, 4138], 'esterases.Appl.': [813], 'Biotechnol.2015doi:10.1007/s00253-015-6422-7Date:': [815], '2015Date': [816], 'accessed:': [817], 'February': [818], '7,': [819], '2015Crossref': [820], '(25)': [822, 2004], '10.Zhang': [825], 'Wu': [827], 'G.': [828, 2402, 2653], 'Liu': [831, 1252, 1605, 1807, 1896], 'Z.': [832, 1218, 1255, 1571, 1608, 1773, 1810, 1899, 2322], 'Aspergillus': [838], 'fumigatus': [839], 'site-directed': [841, 1849], 'mutagenesis.Enzyme': [842], 'Microb.': [843], 'Technol.': [844], '64–65:': [846], '11-16Crossref': [847], 'Better': [853], 'knowledge': [854], 'function': [859], 'will': [860], 'facilitate': [861], 'design.': [863], 'Lipolytic': [864], 'classified': [868], 'into': [869, 1734, 1991, 3197], 'four': [870, 3069, 3090, 3097, 3958], 'blocks': [871], '(C,': [872], 'L,': [873], 'H,': [874], 'X)': [876], 'basis': [879, 1692], 'similarities': [881], 'sequence': [885, 1015, 1127, 3463, 3548, 3845, 3900], 'presence': [888], 'several': [890, 1284, 2466], 'conserved': [891, 1464, 3546, 3700], 'motifs,': [892], 'described': [894, 1757, 1886, 1965], 'ESTHER': [897], 'database': [898, 913], '(11.Lenfant': [899], 'N.': [900, 2408], 'Hotelier': [901], 'Velluet': [903], 'Bourne': [905], 'Marchot': [907], 'P.': [908, 1102, 1484, 2433, 2535, 3331, 3388], 'Chatonnet': [909], 'A.': [910, 1975, 2667, 3337, 3350, 3394, 3400], 'ESTHER,': [911], 'superfamily': [918], 'proteins:': [920], 'tools': [921], 'explore': [923], 'diversity': [924], 'functions.Nucleic': [926], 'Acids': [927, 2542], 'Res.': [928, 2543], '41:': [930], 'D423-D429Crossref': [931], '(203)': [934], 'Block': [937], 'H': [938], 'composed': [940, 1362], 'plant': [942], 'carboxylesterase': [943], 'families.': [948], 'consists': [952], 'lipases': [956], 'distributed': [959], 'across': [960], 'diverse': [961, 1399], 'organisms,': [962], 'including': [963, 1291, 2887], 'animals,': [964], 'plants,': [965], 'microorganisms': [967, 1159], 'Scholar);': [1011], 'they': [1012, 3460, 3841], 'share': [1013, 1355], 'similarity': [1016, 1128, 3303, 3312, 3326, 3383, 3447, 3901], 'mammalian': [1018, 1500], '(12.Holm': [1020], 'Osterlund': [1022, 1473], 'Laurell': [1024], 'H.': [1025, 1210, 1251, 1563, 1604, 1765, 1806, 1895, 2661], 'Contreras': [1026, 1479], 'J.A.': [1027, 1480, 1981], 'mechanisms': [1029], 'regulating': [1030], 'lipolysis.Annu.': [1034], 'Rev.': [1035], 'Nutr.': [1036], '2000;': [1037], '20:': [1038], '365-393Crossref': [1039], '(345)': [1042], 'broad': [1049], 'spectrum,': [1051], 'significant': [1054], 'lipolytic': [1055], 'triacylglycerol,': [1058, 1653], 'diacylglycerol,': [1059], 'monoacylglycerol,': [1060], 'cholesteryl': [1062], 'ester.': [1063], 'Some': [1064], 'playing': [1067], 'role': [1069], 'lipid': [1071], 'catabolism': [1072], 'detoxification': [1074], 'proposed': [1077], '(13.Osterlund': [1078], 'Structure-function': [1080], 'lipase.Eur.': [1084], '2001;': [1087], '268:': [1088], '1899-1907Crossref': [1089], '(57)': [1092], '14.Probst': [1095], 'M.R.': [1096], 'Beer': [1097, 1099], 'Jenö': [1101], 'Meyer': [1103], 'U.A.': [1104], 'Gasser': [1105], 'Human': [1107], 'liver': [1108], 'arylacetamide': [1109], 'deacetylase.': [1110], 'cloning': [1112], 'novel': [1115, 1259, 1547, 1612, 1814, 1903], 'involved': [1117, 3120], 'metabolic': [1120], 'activation': [1121, 1992], 'arylamine': [1123], 'carcinogens': [1124], 'lipase.J.': [1131], '1994;': [1134], '269:': [1135], '21650-21656Abstract': [1136], 'Full': [1137], 'Text': [1138], 'PDF': [1139], 'To': [1143, 4072], 'date,': [1144], 'number': [1146], 'identified': [1153, 2527, 2591, 3527], 'biochemically': [1155], 'characterized': [1156, 1545], 'various': [1158], 'metagenomic': [1161, 1335, 3407], 'libraries': [1162], '15.Liu': [1207], 'Yan': [1211, 1564, 1766], 'Yang': [1213, 1246, 1566, 1599, 1768, 1801, 1890], 'Duan': [1215, 1248, 1568, 1601, 1770, 1803, 1892], 'Jiang': [1217, 1254, 1570, 1607, 1772, 1809, 1898], 'Biochemical': [1219, 1572, 1774], 'fungal': [1224, 1577, 1779], 'miehei': [1228, 1266, 1581, 1619, 1747, 1783, 1821, 1910], 'efficiency': [1231, 1584, 1786], 'synthesis.PLoS': [1234, 1587, 1789], 'One.': [1235, 1588, 1790], '8:': [1237, 1590, 1792], 'e77856Crossref': [1238, 1591, 1793], '(30)': [1241, 1594, 1796], '16.Yan': [1244, 1597, 1799], 'tertiary': [1268, 1621, 1823, 1912, 3905], 'alcohol': [1269, 1622, 1824, 1913], 'hydrolysis': [1270, 1623, 1683, 1825, 1914], 'activity.J.': [1271, 1624, 1826, 1915], '109:': [1277, 1630, 1832, 1921], '76-84Crossref': [1278, 1631, 1833, 1922], 'Moreover,': [1283], 'structurally': [1289], 'elucidated,': [1290], 'PcEst': [1292, 3472], 'Pyrobaculum': [1294], 'calidifontis': [1295, 3332, 3389], '[Protein': [1296], 'Data': [1297, 2686], 'Bank': [1298], '(PDB)': [1299], 'PDB:': [1300], '2YH2],': [1301], 'AfEst': [1302, 3474], '(PDB:': [1306, 1312, 1318, 1324, 1328, 1332], '1JJI),': [1307, 3342, 3399], 'StEst': [1308], 'Sulfolobus': [1310], 'tokodaii': [1311], '3AIK),': [1313], 'AaEst': [1314], 'Alicyclobacillus': [1316], 'acidocaldarius': [1317, 3351, 3401], '1EVQ),': [1319, 3355, 3405], 'BsEst': [1320], 'subtilis': [1323], '1JKM),': [1325], 'EstEl': [1327], '2C7B)': [1329], 'EstE7': [1331], '3DNM)': [1333], 'libraries.': [1336], 'no': [1341, 3899], 'structural': [1342, 1541, 1691, 1710, 1729, 1989, 2388, 2941, 3302, 3311, 3325, 3382, 3485, 3815, 3922, 4016], 'descriptions': [1343], 'fungus.': [1351], 'characteristic': [1357], 'fold,': [1359], 'central': [1365, 1390, 1458, 3246, 3277], 'β-sheet': [1366, 3247, 3278], 'surrounded': [1367, 3253, 3956, 3992], 'both': [1369, 3314], 'sides': [1370], 'α-helices,': [1372], 'serving': [1373], 'stable': [1376], 'core.': [1378], 'substitutions,': [1382], 'loop': [1383, 2250, 3492, 3967, 4008], 'insertions,': [1384], 'deletions': [1386], 'occurring': [1387], 'cores': [1391], 'during': [1392], 'led': [1395], 'functions': [1401], '(17.Rozeboom': [1402], 'H.J.': [1403], 'Godinho': [1404], 'L.F.': [1405], 'Nardini': [1406], 'Quax': [1408], 'W.J.': [1409], 'carboxylesterases': [1417], 'enantioselectivities.Biochim.': [1420], '567-575Crossref': [1425], '(18)': [1428], 'α/β': [1435, 1522, 1666], 'triad': [1442, 3497], 'made': [1443, 3879], 'up': [1444, 3880], 'nucleophile': [1447, 1459, 3535, 3558, 3642], '(Ser),': [1448], 'an': [1449, 2162, 3076, 3239, 3271, 3293, 3829], '(Asp': [1451], 'Glu),': [1453], 'His.': [1456], 'within': [1462, 3544], 'G-X-S-X-G': [1465], 'motif': [1466, 3687], '"nucleophile': [1469], 'elbow"': [1470], '(18.Wei': [1471], 'Matern': [1475], 'U.': [1476, 1482], 'Holm': [1477], 'Derewenda': [1481, 1487], 'Sheffield': [1483], 'Kneusel': [1485], 'R.E.': [1486], 'Z.S.': [1488], 'brefeldin': [1492], 'esterase,': [1494], 'bacterial': [1496], 'homolog': [1497], 'lipase.Nat.': [1502], '6:': [1506], '340-345Crossref': [1507], '(145)': [1510], 'Though': [1513], 'nearly': [1518], 'identical': [1519], 'all': [1521, 2461, 2745, 2821], 'specificities': [1526, 4086, 4168, 4175, 4189], 'very': [1528], 'different.': [1529], 'reasons': [1531], 'phenomenon': [1534, 3679], 'not': [1536, 3022, 3681], 'elucidated': [1538], 'level.': [1542], 'We': [1543], 'miehei:': [1556], '(15.Liu': [1560, 1762], 'physiological': [1637], 'detected': [1643], 'our': [1645, 1759], 'studies': [1646, 1761, 4186, 4200], 'linalyl': [1654], 'acetate,': [1655], 'butyl': [1657], 'butyrate.': [1658], 'Both': [1659], 'these': [1661, 3480], 'belong': [1663], 'hydrolases': [1667], 'specificities:': [1672], 'shows': [1674], 'highest': [1675], 'longer-chain': [1678], 'esters,': [1679], 'whereas': [1680, 4158], 'favors': [1682], 'shorter-chain': [1685], 'esters.': [1686], 'Here,': [1687], 'decipher': [1689], 'we': [1698], 'detail': [1708], 'differences.': [1711], 'work': [1713], 'attempt': [1717], 'characterize': [1719], 'level': [1730], 'gain': [1732], 'insights': [1733, 1990], 'structure–function': [1736], 'relationships.': [1737], 'recombinant': [1740], 'CAU432': [1748], 'cloned,': [1750], 'expressed,': [1751], 'purified': [1753, 1884], 'according': [1754, 2866], 'protocols': [1756], 'previous': [1760, 4185, 4199], 'Site-directed': [1838], 'mutations': [1839], 'generated': [1843], 'Fast': [1846], 'Mutagenesis': [1847], 'System': [1848], 'mutagenesis': [1850], 'kit': [1851], '(TransGen': [1852], 'Biotech,': [1853], 'China).': [1854], 'mutation': [1857, 1869], 'W92F,': [1858], 'primers': [1860, 1872], '5′-TTCCATGGTGGAGGTTTTGTTGTTGGCAG-3′': [1862], '(forward)': [1863, 1875], '5′-AAAACCTCCACCATGGAAGAAGACGATAGG-3′': [1865], '(reverse).': [1866, 1878], 'F222A,': [1870], '5′-CAAGCTGATGGTTTGGTTTGCTGATCACTATAT-3′': [1874], '5′-GCAAACCAAACCATCA\xadG\xadCTTGCGTGTGAGATAAT-3′': [1877], 'variants': [1880], 'expressed': [1882, 2137], 'previously': [1887], '(16.Yan': [1888], 'purity': [1928], 'proteins': [1931], 'was': [1932, 1962, 2025, 2053, 2057, 2066, 2087, 2101, 2123, 2136, 2241, 2347, 2373, 2646, 2916, 2999, 3060, 3087, 3146, 3160, 3178, 3526, 3542, 3680, 3705, 3715, 3866, 3953, 3977, 3991, 4108], 'checked': [1933], 'SDS-PAGE.': [1935], 'Prior': [1936], 'crystallization,': [1938], 'samples': [1941], 'moved': [1943], '20': [1945, 2046], 'mM': [1946, 1953, 2032, 2047, 2074, 2873], 'Tris-HCl': [1947, 2033, 2874], 'buffer': [1948, 2034, 2076, 2192, 2224, 2875], '8.0': [1950], 'containing': [1951, 2079, 2177, 2212], '100': [1952], 'NaCl': [1954], 'concentrated': [1956], '15': [1958], 'mg/ml.': [1959], 'Esterase': [1960], 'Gutiérrez-Fernández': [1967], 'et': [1968, 2670], 'al.': [1969], '(19.Gutiérrez-Fernández': [1970], 'Vaquero': [1972], 'M.E.': [1973], 'Prieto': [1974], 'Barriuso': [1976], 'Martinez': [1978], 'M.J.': [1979], 'Hermoso': [1980], 'Ophiostoma': [1985], 'piceae': [1986], 'sterol': [1987], 'esterase:': [1988], 'product': [1995], 'release.J.': [1996], '187:': [2000], '215-222Crossref': [2001], 'Scholar)': [2006, 2429, 2456], 'using': [2007, 2129, 2247, 2317, 2355, 2377, 2394, 2475, 2880], 'p-nitrophenyl': [2008, 2889, 2892, 2895, 2898, 2901, 2904, 2908], 'acetate': [2009], '(pNPA)': [2010], 'minor': [2015], 'modifications.': [2016], 'Briefly,': [2017], '50': [2018, 2031, 2043, 2872], 'μl': [2019, 2029, 2044, 2071], 'suitably': [2021], 'diluted': [2022], 'solution': [2024, 2160, 2176, 2211, 2258, 2261], 'prepared': [2026, 2627], '400': [2028], '7.5,': [2036], 'after': [2038, 2232], 'preheating': [2039], '2': [2041, 2200], 'min,': [2042], 'pNPA': [2048], '(in': [2050], 'pure': [2051], 'isopropanol)': [2052], 'added.': [2054], 'mixture': [2056], 'incubated': [2058], '50°C': [2060, 2879], 'min.': [2063], 'reaction': [2065], 'stopped': [2067], 'adding': [2069], '500': [2070], '300': [2073], 'phosphate': [2075], '7.0': [2078], '5%': [2080, 2767, 2834], '(w/v)': [2081, 2179, 2214], 'SDS.': [2082], 'released': [2084], 'p-nitrophenol': [2085], '(pNP)': [2086], 'quantified': [2088], 'measuring': [2090], 'absorbance': [2092], '410': [2094], 'nm.': [2095], 'One': [2096, 2911], 'unit': [2097, 2912, 3007], 'defined': [2102, 2917], 'amount': [2105, 2920], 'required': [2108, 2923], 'liberate': [2110], '1': [2111, 2157], 'μmol': [2112, 2927], 'pNP': [2113, 2882, 2929], 'per': [2114, 2140, 2930], 'minute': [2115, 2931], 'under': [2116, 2932, 2955], 'above': [2118, 2934], 'assay': [2119, 2870, 2935], 'conditions.': [2120, 2936], 'Protein': [2121], 'concentration': [2122], 'measured': [2124], 'Lowry': [2127], 'method': [2128, 2151, 2354], 'BSA': [2130], 'standard.': [2133], 'Specific': [2134], 'units': [2139], 'milligram': [2141], 'protein.': [2142], 'Proteins': [2143], 'crystallized': [2145], 'sitting-drop': [2148], 'vapor': [2149], 'diffusion': [2150], '293': [2153], 'K': [2154], 'mixing': [2156], 'µl': [2158], 'equal': [2163], 'volume': [2164], 'reservoir': [2166, 2175, 2210], 'solution.': [2167], 'Crystals': [2168, 2203], 'obtained': [2172, 2207], '25%': [2178], 'polyethylene': [2180], 'glycol': [2181], '(PEG)': [2182], '3350': [2183], '0.2': [2185], 'M': [2186, 2190, 2222], '(NH4)2SO4': [2187], '0.1': [2189, 2221], 'MES': [2191], '6.0.': [2194], 'crystals': [2197, 2229, 2287], 'observed': [2199, 2231], 'days': [2201], 'later.': [2202], '20%': [2213, 2264], 'PEG4000': [2215], '10%': [2217], '(v/v)': [2218], '2-propanol': [2219], 'HEPES': [2223], '7.5.': [2226], '7': [2233, 3673], 'days.': [2234], 'X-ray': [2236, 2276], 'diffraction': [2237, 2277, 2310, 2328], 'experiments,': [2238], 'each': [2239, 3262, 3290], 'fished': [2242], 'crystallization': [2245, 2260], 'drop': [2246], 'nylon': [2249], '(Hampton': [2251], 'Research),': [2252], 'soaked': [2253], 'briefly': [2254], 'cryoprotectant': [2257], '(the': [2259], 'supplemented': [2262], 'v/v': [2265], 'glycerol),': [2266], 'then': [2268], 'flash-cooled': [2269], 'liquid': [2271], 'nitrogen': [2272, 3737], '70': [2274], 'K.': [2275, 2439], 'data': [2278, 2311, 2329, 2462, 2983], 'collected': [2284, 2330], 'single': [2286, 3058], 'beamline': [2289, 2299], '3W1A': [2290], 'Beijing': [2293], 'Synchrotron': [2294, 2304], 'Radiation': [2295], 'Facility': [2296, 2306], '(BSRF)': [2297], 'BL-17U': [2300], 'Shanghai': [2303], 'Research': [2305], '(SSRF),': [2307], 'All': [2309], 'indexed,': [2313], 'integrated,': [2314], 'scaled': [2316], 'program': [2319, 2564, 2600], 'HKL-2000': [2320], '(20.Otwinowski': [2321], 'Minor': [2323], 'W.': [2324, 2602], 'Processing': [2325], 'x-ray': [2327], 'oscillation': [2332], 'mode.Methods': [2333], 'Enzymol.': [2334], '1997;': [2335], '276:': [2336], '307-326Crossref': [2337], '(38526)': [2340], 'molecular': [2351], 'replacement': [2352], '(MR)': [2353], 'PDB': [2359, 2954, 3334, 3340, 3346, 3353, 3391, 3397, 3403, 3410], 'entry': [2360], '1JJI': [2361], '(Archaeoglobus': [2362], 'carboxylesterase)': [2364], 'search': [2367, 2385, 3308], 'model.': [2368, 2386], 'solved': [2374], 'MR': [2376], 'refined': [2379, 2393], 'models': [2389, 2484], 'built': [2391], 'Phenix': [2396], '(21.Adams': [2397], 'P.D.': [2398], 'Afonine': [2399], 'P.V.': [2400], 'Bunkóczi': [2401], 'Chen': [2403], 'V.B.': [2404, 2492], 'Davis': [2405], 'I.W.': [2406], 'Echols': [2407], 'PHENIX:': [2409], 'comprehensive': [2411], 'Python-based': [2412], 'system': [2413], 'macromolecular': [2415, 2508], 'solution.Acta': [2417], 'Crystallogr.': [2418, 2421, 2445, 2448, 2510, 2513, 2576, 2579], 'D': [2419, 2446, 2511, 2577], '2010;': [2422, 2449, 2514, 2544], '66:': [2423, 2450, 2515], '213-221Crossref': [2424], '(16434)': [2427], 'Coot': [2431], '(22.Emsley': [2432], 'Lohkamp': [2434], 'Scott': [2436], 'W.G.': [2437], 'Cowtan': [2438], 'Features': [2440], 'development': [2442], 'Coot.Acta': [2444], '486-501Crossref': [2451], '(17075)': [2454], 'programs.': [2457], 'R': [2458], 'values': [2459, 3363, 3420], 'reduced': [2464], 'cycles': [2467], 'simulated': [2469], 'annealing,': [2470], 'minimization,': [2471], 'B-factor': [2473, 2789], 'refinement': [2474, 2689, 2699, 2987], 'Phenix.refine': [2476], 'followed': [2477], 'manual': [2479], 'model': [2480], 'rebuilding.': [2481], 'final': [2483], 'analyzed': [2486], 'validated': [2488], 'MolProbity': [2490], '(23.Chen': [2491], 'Arendall': [2493], 'W.B.': [2494], 'Headd': [2495], 'J.J.': [2496], 'Keedy': [2497], 'D.A.': [2498], 'Immormino': [2499], 'R.M.': [2500], 'Kapral': [2501], 'G.J.': [2502, 2566], 'MolProbity:': [2503], 'all-atom': [2504], 'validation': [2506], 'crystallography.Acta': [2509], '12-21Crossref': [2516], '(9814)': [2519], 'homologs': [2523], 'DALI': [2530, 3307], 'server': [2531], '(24.Holm': [2532], 'Rosenstrom': [2534], 'Dali': [2536], 'server:': [2537], 'conservation': [2538], 'mapping': [2539], '3D.Nucleic': [2541], '38:': [2545], 'W545-W549Crossref': [2546], '(3053)': [2549], 'superpositions': [2553], 'RMSD': [2555, 3359, 3416, 3831], '(root-mean-square': [2556], 'deviation)': [2557], 'calculation': [2558], 'performed': [2560], 'LSQMAN': [2563], '(25.Kleywegt': [2565], 'Experimental': [2567], 'assessment': [2568], 'differences': [2570, 3486, 3923, 4017], 'related': [2572, 3265], 'structures.Acta': [2575], '55:': [2581], '1878-1884Crossref': [2582], '(133)': [2585], 'Secondary-structure': [2588], 'elements': [2589, 3865], 'DSSP': [2594], '(Define': [2595], 'Secondary': [2596], 'Structure': [2597], 'Proteins)': [2599], '(26.Kabsch': [2601], 'Sander': [2603], 'Dictionary': [2605], 'secondary': [2608], 'structure:': [2609], 'pattern': [2610], 'recognition': [2611], 'hydrogen-bonded': [2613], 'geometrical': [2615], 'features.Biopolymers.': [2616], '1983;': [2617], '22:': [2618], '2577-2637Crossref': [2619], '(12266)': [2622], 'Figures': [2625], 'PyMOL': [2629, 2634], '(27.DeLanoW.': [2630], 'L..': [2631], '2012.': [2632], 'Graphics': [2636], 'System,': [2637], 'Version': [2638], '1.5.0.4.': [2639], 'Schrodinger,': [2640], 'New': [2641], 'York.Google': [2642], 'Sequence': [2644], 'alignment': [2645], 'created': [2647, 3716], 'ClustalW': [2649], '(28.Larkin': [2650], 'M.A.': [2651], 'Blackshields': [2652], 'Brown': [2654], 'N.P.': [2655], 'Chenna': [2656], 'McGettigan': [2658], 'P.A.': [2659], 'McWilliam': [2660], 'Valentin': [2662], 'F.': [2663, 3758], 'Wallace': [2664], 'I.M.': [2665], 'Wilm': [2666], 'Lopez': [2668], 'al.Clustal': [2671], 'W': [2672], 'Clustal': [2674], 'X': [2675], 'version': [2676], '2.0.Bioinformatics.': [2677], '23:': [2679], '2947-2948Crossref': [2680], '(22511)': [2683], 'collection': [2687, 2697, 2984], 'statistics': [2690, 2981], 'given': [2692], 'Table': [2694, 2991], '1.TABLE': [2695], '1.Data': [2696], 'statisticsData': [2700], 'Collection': [2701], 'StatisticsRmEstARmEstBWavelength': [2702], '(Å)0.97910.9792Resolution': [2703], '(Å)31.85–2.41': [2705], '(2.50–2.41)46.65–2.27': [2706], '(2.35–2.27)Space': [2707], 'groupP1C121Unit': [2708], 'cell': [2709], 'parametersa,': [2710], 'b,': [2711], 'c': [2712], '(Å)56.69,': [2713], '64.52,': [2714], '64.79151.13,': [2715], '49.68,': [2716], '183.21Unique': [2717], 'reflections26,749': [2718], '(2,373)55,910': [2719], '(4,188)Completeness': [2720], '(%)97.31': [2721], '(86.07)94.49': [2722], '(71.8)Rmerge': [2723], '(%)aRmerge': [2724], '=': [2725, 2761, 2801, 2828], 'ΣhklΣi[Ii(hkl)': [2726, 2762, 2802, 2829], '−': [2727, 2763, 2803, 2830], 'I(hkl)]/ΣhklΣiIi(hkl),': [2728, 2804], 'where': [2729, 2805, 3859], 'Ii(hkl)': [2730, 2806], 'ith': [2733, 2809], 'observation': [2734, 2810], 'reflection': [2736, 2749, 2812, 2825], 'hkl': [2737, 2813], 'andI(hkl)': [2738, 2814], 'weighted': [2741, 2817], 'average': [2742, 2818], 'intensity': [2743, 2819], 'observations': [2746, 2822], 'i': [2747, 2823], 'hkl.5.1': [2750], '(35.6)7.5': [2751], '(46.6)I/σ(I)14.22': [2752], '(3.54)15.08': [2753], '(8.28)Wilson': [2754], 'B-factor34.2923.20Refinement': [2755], 'statisticsResolution': [2756], '(Å)2.412.27Rwork': [2758], '(%)/Rfree': [2759], '(%)bRmerge': [2760], 'I(hkl)]/ΣhklΣiIi(hkl);': [2764, 2831], '95%': [2765, 2832], 'reflections': [2769, 2836], 'Rwork': [2773, 2840], 'Rfree,': [2775, 2842], 'respectively.17.75/22.5618.84/23.59No.': [2776], 'atoms5,14310,735No.': [2777], 'residues6401,294No.': [2778], 'water': [2779], 'molecules269529RMSDBond': [2780], 'lengths': [2781, 4037], '(Å)0.0110.010Bond': [2782], 'angles': [2783], '(°)1.301.24Ramachandran': [2784], 'favored': [2785], '(%)9696Ramachandran': [2786], 'outliers': [2787], '(%)0.310.16Average': [2788], '(Å2)31.9028.40PDB': [2790], 'code4WY54WY8Values': [2791], 'parenthesis': [2793, 2852], 'last': [2797, 2856], 'shell.a': [2799], 'Rmerge': [2800, 2827], 'hkl.b': [2826], 'Open': [2844], 'table': [2845], 'tab': [2849], 'Values': [2850], 'shell.': [2858], 'Substrate': [2859], 'investigated': [2865], 'standard': [2868], '7.5': [2877], 'substrates,': [2886], 'pNPA,': [2888], 'butyrate': [2890], '(pNPB),': [2891], 'hexanoate': [2893], '(pNPH),': [2894], 'caprylate': [2896], '(pNPC),': [2897], 'decanoate': [2899], '(pNPD),': [2900], 'laurate': [2902], '(pNPL),': [2903], 'myristate': [2905], '(pNPM)': [2906], 'palmitate': [2909], '(pNPP).': [2910], 'release': [2925], '1.0': [2926], 'atomic': [2938], 'coordinates': [2939], 'factors': [2942], 'deposited': [2951], 'accession': [2956], 'numbers': [2957], '4WY5': [2958], '4WY8,': [2960], '2.41': [2972], '2.27': [2975], 'crystallographic': [2980], 'summarized': [2989], '1.': [2992], 'triclinic': [2994], 'space': [2995, 3083], 'group': [2996, 3084], 'P1': [3000], 'monomers': [3003, 3049, 3074, 3098], 'asymmetric': [3006, 3030, 3094], '(Fig.': [3008, 3052, 3079, 3114, 3169, 3186, 3256, 3297, 3531, 3733, 3821, 3930, 4013, 4042], '1A).': [3009], 'Amino': [3010], '1–3': [3013], 'uncleaved': [3018], 'C-terminal': [3019], 'His-tag': [3020], 'visible': [3023], 'electron-density': [3026], 'map.': [3027], 'unit,': [3031], 'formed': [3033, 3903], 'dimer': [3035], 'complex': [3037], 'three': [3039], 'sulfates,': [3040], 'sulfates': [3045], 'interacted': [3046], 'via': [3050, 3110], 'Arg104': [3051], '1B).': [3053], 'Note': [3054, 3436], 'third': [3057], 'sulfate': [3059], 'crevice': [3064], 'monomers,': [3067], 'Arg': [3070], 'forming': [3075], 'arched': [3077], 'area': [3078], '1C).': [3080], 'monoclinic': [3082], 'C121': [3088], 'molecules': [3091], 'unit.': [3095], 'arranged': [3100], 'dimers': [3104], 'form': [3107, 3270], 'tetramer': [3109], 'hydrogen': [3111, 3117, 3135, 3148, 3162, 3609, 3664, 3744], 'bonding': [3112], 'network': [3113, 3662], '1D).': [3115], 'Four': [3116], 'tetramer:': [3126], 'Ile39': [3127], 'Asp40': [3129], 'chain': [3131, 3140, 3144, 3153, 3158, 3167, 4036], 'directly': [3134, 3147, 3161], 'bonded': [3136, 3149, 3163], 'Lys149': [3138], 'C,': [3141, 3154], 'Glu55': [3142], 'Asp75': [3151], 'Val51': [3156], 'Gln79': [3165], 'C': [3168], '1E).': [3170], 'overall': [3172, 3477, 3830], 'monomer': [3177, 3185, 3263], 'analogous': [3179], '2).': [3187, 3257, 3298, 4136], 'divided': [3196], 'domains:': [3199], '(residues': [3203, 3219, 3688], '51–191': [3204, 3210], '253–322': [3206, 3212], '4–51': [3220, 3871], '207–247': [3222], '3–51': [3226, 3876], '206–247': [3228], 'RmEstB).': [3230, 3648], 'domains': [3233, 3883, 3897], 'had': [3234], 'architecture': [3237], 'eight': [3249], 'mostly': [3250], 'parallel': [3251], 'strands': [3252, 3288], 'α-helices': [3255, 3959, 3995], 'core': [3259, 3857], 'β-sheets': [3260], '2-fold': [3267], 'symmetry': [3268], 'extended': [3272, 3937], 'intermolecular': [3273], '16-stranded': [3274], 'β-sheet.': [3275], 'displayed': [3279, 3380], 'left-handed': [3281], 'superhelical': [3282], 'twist,': [3283], 'β1': [3285], 'β8': [3287], 'crossing': [3289], 'other': [3291, 3319, 3452], 'angle': [3294], '∼120°': [3296], 'analysis': [3300, 3812], 'carried': [3304], 'out': [3305], 'suggested': [3309, 3917], 'exhibited': [3323], '(PcEst,': [3333, 3390], 'code:': [3335, 3341, 3347, 3354, 3392, 3398, 3404, 3411], '3ZWQ),': [3336, 3393], '(AfEst,': [3339, 3396], 'Salmonella': [3343], 'typhimurum': [3344], '(StEst,': [3345], '3GA7),': [3348], '(AaEst,': [3352, 3402], 'Z-score': [3357, 3414], 'Cα': [3361, 3418, 3838], 'atom': [3362, 3419, 3622, 3631], '39.7': [3365], '1.9,': [3367, 3427], '39': [3368], '2.0,': [3370], '38.8': [3371], '2.1,': [3373, 3430], '38.2': [3375], '2.2,': [3377], 'library': [3408], '(EstE1,': [3409], '2C7B),': [3412], '41.5': [3422], '1.8,': [3424], '40.5': [3425], '40.3': [3428, 3432], '1.7,': [3434], 'three-dimensional': [3439], 'shared': [3445, 3842, 3898], 'those': [3449], 'members': [3453], 'family,': [3458, 3704], 'though': [3459, 3840], 'identities': [3464], '(<40%).': [3465], 'Superimposing': [3466], 'RmEstA/RmEstB': [3467], 'similar': [3476, 3904, 4141], 'folds': [3478], 'mainly': [3489], 'regions.': [3493], 'Ser161': [3500, 3536, 3624, 3643, 3804], '(Ser164': [3501, 3539, 3625, 3646], 'nucleophile,': [3506], '(His291': [3508, 3634], 'proton': [3513], 'acceptor/donor,': [3514], 'Asp262': [3516, 3655], '(Asp261': [3517], 'residue': [3522, 3947], 'stabilizing': [3523, 3790], 'His': [3525], '(RmEstB)': [3530], '3).': [3532, 3734], 'key': [3534], 'pentapeptide': [3547], 'Gly-X-Ser-X-Gly,': [3549], 'apex': [3555], 'elbow,': [3559], 'sharp': [3561], 'turn': [3562], 'connecting': [3563], 'β5': [3564], 'α6': [3566], 'bond': [3610], '(2.7': [3611], '2.4': [3615], 'Oγ': [3621], 'Nε2': [3630], 'stabilized': [3637, 3659], 'conformation': [3639], 'side': [3650], 'chains': [3651], 'carboxyl': [3669], 'edge': [3670], 'β-strands': [3672], '8,': [3675], 'RmEstB.': [3684, 3810, 4071], 'His-Gly-Gly-Gly': [3686], '87–90': [3689], '88–91': [3694], 'RmEstB),': [3696], 'usually': [3699], 'upstream': [3707, 3884], 'active': [3710], 'sites.': [3711], 'oxyanion': [3713, 3741], 'hole': [3714, 3742], 'Gly89,': [3719], 'Gly90,': [3720, 3727], 'Ala162': [3722], 'Gly91,': [3728], 'Ala165': [3730], 'main-chain': [3736], 'atoms': [3738], 'donate': [3743], 'cleaved': [3747], '(29.Mandrich': [3749], 'Merone': [3751], 'Pezzullo': [3753], 'Cipolla': [3755], 'Nicotra': [3757], 'Rossi': [3759], 'Role': [3761], 'N': [3764], 'terminus': [3765], 'activity,': [3768], 'belonging': [3775], 'family.J.': [3779], '2005;': [3782], '345:': [3783], '501-512Crossref': [3784], 'Scholar),': [3789], 'negative': [3792], 'charges': [3793], 'tetrahedral': [3796], 'intermediates': [3797], 'arising': [3798], 'nucleophilic': [3801], 'attack': [3802], 'Ser164': [3808], 'Superposition': [3811, 3907], 'homogeneity': [3816], '4A).': [3822], 'Superpositioning': [3823], 'onto': [3826], 'exhibits': [3828], '1.41': [3833], '311': [3836], 'corresponding': [3837], 'atoms,': [3839], 'only': [3843], '46%': [3844], 'identity.': [3846], 'folding': [3848], 'patterns': [3849], 'presented': [3854], 'common': [3856], 'assignment': [3861], 'secondary-structure': [3864], 'almost': [3867, 4163], 'same.': [3869], 'Residues': [3870], 'catalysis': [3888], 'domains.': [3889], 'It': [3890], 'interesting': [3892], 'but': [3902], 'structures.': [3906], 'surface': [3910, 3943], 'sticks': [3914], 'most': [3920], 'striking': [3921], 'localized': [3925], 'pockets': [3929, 4021], '4B).': [3931, 4014], '∼11': [3938], 'Ser164.': [3948], 'deep': [3950], 'hydrophobic': [3951], 'cleft': [3952], 'funnel-shaped': [3954], '(α1,': [3960, 3996], 'α2,': [3961, 3997], 'α6,': [3962, 3998], 'α8)': [3964], 'regions': [3968, 4009], '(His88–Gly91': [3969], 'Ile290–Ala297).': [3971], 'channel': [3979, 3990], 'running': [3980], 'through': [3981], 'whole': [3983], 'entrance': [3987], 'five': [3994], 'α8,': [3999], 'α9),': [4001], '310-helix': [4003], 'G2': [4004], '(His197–Lys199)': [4005], '(His87–Gly90': [4010], 'Ile291–Ala298)': [4012], 'contribute': [4030], 'difference': [4033], '4).': [4043], '(Phe222': [4048], 'Trp92),': [4050], 'block': [4062], 'narrow': [4066], 'validate': [4073], 'speculation,': [4075], '(RmEstB-F222A': [4078], 'RmEstB-W92F)': [4080], 'designed,': [4082], 'determined.': [4091], 'Compared': [4092], 'wild-type': [4097], 'enzyme,': [4098], 'specific': [4100, 4113, 4144], 'RmEstB-F222A': [4105, 4116], 'C2': [4107, 4161], 'slightly': [4109], 'decreased.': [4110], 'relatively': [4120], 'longer': [4121], 'chains,': [4122], 'C4': [4125, 4149], 'C6,': [4127], 'increased': [4128, 4152], 'significantly': [4129], '1.65-': [4131], '1.4-fold,': [4133], 'respectively': [4134], '(Table': [4135, 4165], 'RmEstB-W92F': [4139, 4147], 'results:': [4142], 'activities': [4145], 'C6': [4151], '1.33-': [4154], '1.11-fold,': [4156], 'respectively,': [4157], 'remained': [4162], 'unchanged': [4164], '2).TABLE': 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{'year': 2020, 'cited_by_count': 4}, {'year': 2019, 'cited_by_count': 5}, {'year': 2018, 'cited_by_count': 3}, {'year': 2017, 'cited_by_count': 5}, {'year': 2016, 'cited_by_count': 4}, {'year': 2015, 'cited_by_count': 1}], 'updated_date': '2025-01-20T15:51:00.801456', 'created_date': '2016-06-24'}
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