Title: Presence of cholinomimetic and calcium channel blocking constituents in Carthamus oxycantha
Abstract: The crude extract of Carthamus oxycantha (Co.Cr) was studied in vitro for its possible spasmogenic and spasmolytic activities.
Segments of isolated rabbit jejunum 2-3 cm long were mounted in Tyrode’s solution and aerated with 95% oxygen in carbon dioxide. Isotonic responses were measured on Bioscience oscillograph. Isotonic responses were measured on Bioscience oscillograph.
Co.Cr caused dose-dependent (0.03-3.0 mg/mL) spasmogenic effect in spontaneously contracting rabbit jejunum, followed by relaxation at the next higher doses of 5.0-10.0 mg/mL. In the presence of atropine (0.03 µM), spasmogenic effect was blocked and the relaxant effect was observed at lower doses (0.03-5.0 mg/mL). Co.Cr (1.0-10.0 mg/mL) produced relaxation of high K+ (80 mM)-induced contraction, suggestive of calcium channel blockade. The CCB effect was confirmed when Co.Cr in the atropinized tissue produced a dose-dependent shift in the Ca++ dose-response curves to the right, similar to that of verapamil, a standard calcium channel blocker.
These data indicate that Carthamus oxycantha exhibits spasmogenic effect through cholinergic action and the spasmolytic effect through calcium channel blockade.
Publication Year: 2010
Publication Date: 2010-11-20
Language: en
Type: article
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