Title: Cytochrome P4501A indices as biomarkers of contaminant exposure: A field study with plaice and flounder
Abstract:Samples of two flatfish species, flounder (Platichthys flesus) and plaice (Pleurnectus platessa), were collected during August/September 1991 and May/June 1992 in the southern North Sea. The induction...Samples of two flatfish species, flounder (Platichthys flesus) and plaice (Pleurnectus platessa), were collected during August/September 1991 and May/June 1992 in the southern North Sea. The induction of hepatic cytochrome P4501A1 (CYP1A) in both species was examined, when exposed to environmental PCBs and PAHs. CYP1A was measured by a semi-quantitative ELISA technique and by the activity of its catalyst, 7-ethoxyresorufin O-deethylase (EROD). In plaice, both hepatic CYP1 protein level and EROD activity were significantly higher at offshore sampling locations than at coastal locations, by a factor of 1.5 to 2. In flounder, which were only collected at coastal locations, the range of values of EROD activity was much greater than in plaice, but the range in CYP1A protein content was comparable in both species. multiple regression analysis of data from plaice and flounder did not show any significant correlation between CYP1A indices, water temperature and contaminant concentrations in fish tissues. CYP1A indices also appeared to be unrelated to food type, as determined by visual screening of fish intestines. It is concluded that the induction of CYP1A in plaice and flounder from the southern North Sea is not related in a simple manner to exposure to PCBs and PAHs. From themore » point of view-of use as a biomarker, the measurement of CYP1A protein level and EROD activity in plaice and flounder from the open North Sea cannot be applied straightforwardly for monitoring exposure to PCBs and/or PAHs.« lessRead More
Publication Year: 1995
Publication Date: 1995-12-31
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot