Title: EVALUATION OF ANTIFUNGAL ACTIVITY OF SOLANUM NIGRUM AGAINST ASPERGILLUS NIGER, THE CAUSE OF BLACK ROT DISEASE OF ONION
Abstract: Antifungal activity of methanolic leaf and root extracts of Solanum nigrum L. was tested against Aspergillus niger Teigh. that causes black rot disease of onion ( Allium cepa L.). Different concentrations of methanol leaf and root extracts viz. 1%, 2%, 3%, 4% and 5% were applied in vitro against A. niger . Root extract was proved more antifungal than leaf extract as its 5% concentration reduced the test fungal growth by 30%. Methanol root extract of S. nigrum was selected for further fractionation and this was partitioned by using various organic solvents viz. n -hexane, chloroform, ethyl acetate and n -butanol. MIC (minimum inhibitory concentration) of the isolated fractions along with a commercial fungicide (metalaxyl+mancozeb) was checked by serial dilution method against A. niger. Various concentrations (0.20 mg mL -1 to 0.0007 mg mL -1 ) were used for MIC bioassay and data was recorded after 24, 48 and 72 h incubation period. Chloroform fraction and synthetic fungicide more effectively retarded spore germination of A. niger with MIC 0.0007 mg mL -1 . The other fractions were comparatively less antifungal and their order of effectiveness was ethyl acetate > n -hexane> n -butanol.
Publication Year: 2015
Publication Date: 2015-01-01
Language: en
Type: article
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Cited By Count: 1
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