Title: Validated RP-HPLC Method for the Quantitation of Candesartan in Bulk and Pharmaceutical Dosage Forms.
Abstract: A simple, specific, accurate, precise and sensitive Reverse Phase High Performance Liquid Chromatographic method has been developed for the quantitation of candesartan in both pure and pharmaceutical dosage forms. An Inertsil ODS-3V C-18, 5 um column having 250 x 4.6 mm internal diameter in isocratic mode with mobile phase containing ortho phosphoric acid: Acetonitrile (20:80v/v) and adjust the pH to 3.2 by using 0.03M potassium hydrogen phosphate buffer. The flow rate was 1.0 ml/min and the effluents were monitored at 210 nm. The retention time was 8.057min. The linearity was in the range of 50–160 μ g/ml. This method was validated for linearity, precision, limit of detection, limit of quantitation and accuracy. Statistical analysis proves that the method is reproducible and selective for the estimation of the said drug.
Publication Year: 2011
Publication Date: 2011-06-28
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot