Title: The Effects of Brussels Sprouts and Their Components, Indole-3-Carbinol (I3C) and l-Cyano-2-HydroxY-3-Butene (CHB) on Hepatic Detoxification Enzyme Activitles
Abstract: Background. Brussels sprouts, one of many cruciferous vegetables, have been shown to have chemoprevention effects in both animal and human studies. The mechanism of chemoprevention activity is associated with the induction of drug-metabolizing enzymes, which modulate carcinogen metabolism. Whether indole-3 carbinol (I3C) and l-cyano-2 hydroxY-3-butene (CHB), two major components in Brussels sprouts, contribute to the inductions of drug-metabolizing enzymes has not been determined yet. Therefore, the chemopreventive effects of Brussels sprouts and their components were separately investigated in 2 experiments, by our group. Methods. Freeze-dried Brussels sprouts (BS) powder at 20% diet (w /w) was fed to mice and rats (4/group) for 7 days. Pair-fed animals were used as the control group. Results. Significant inductions were shown in both phase II enzymes, such as glutathione Stransferase (GST) and quinone reductase (QR), and phase I enzymes, such as cytochrome P450。lA (CYP IA), in the mouse-and rat groups compared to .their pair-fed groups, In the second study, the treatment diet included 6.25mg 13C/g diet, 4 mg CHB/g diet, and 1% butylated hydroxyanisole (BHA) as a positive control. Conclusions. No significant glutathione (GSH) induction was seen in any treatment group.13C produced significant induction in GST, QR and CYPIA which were 2.4-, 2.4-,and 5.6-fold, respectively, over the control group. However, CHB did not show any significant inductive effects. Therefore, we concluded that 13C, not CHB, at least in part, contributes to the chemopreventive effect of inducing drug-metabolizing enzymes in Brussels sprouts. ( Mid Taiwan J Med 2002;7:28-37)
Publication Year: 2002
Publication Date: 2002-03-01
Language: en
Type: article
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