Abstract: [Objective]The aim was to study the tissue culture of mini-Papaya.[Method]The pretreatment seeds of mini-Papaya were cul-tured in the MS medium containing 6-BA and IBA of different densities for rapid propagation.[Result]In the condition of aseptic strain,the surface of mini-Papaya peel was uniformly wiped by 75% alcohol,then seeds were removed and washed by aseptic water for 3 times,which was the best sterilization method,and the pollution rate of seed was only 2.52% .After seeds which had been soaked by the equivolume mix-solution of 1 000 mg/L GA3 and 1 mg/L 6-BA for 18 h were further purified in MS medium,the germination rate of seed,the length of germ and radical and the height of seedling were 68.42% ,2.25 cm,0.80 cm and 1.52 cm,respectively,furthermore the total situation of seedling growth was better.When subculture multiplication medium was MS + 0.5mg/L 6-BA + 0.1 mg/L IBA medium,proliferation coefficient of sub-culture multiplication reached the highest (7.92) ,and the seedings grew better.The ratio of vitrified shoots decreasing with the increase of light intensity could reach the lowest level (3.21% ) under the light intensity of 3 000 lx.[Conclusion]The research provides reference for studies on tissue culture and rapid propagation of mini-Papaya.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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