Get quick answers to your questions about the article from our AI researcher chatbot
{'id': 'https://openalex.org/W2260059978', 'doi': 'https://doi.org/10.1371/journal.pone.0148028', 'title': 'Comparing Apples and Oranges?: Next Generation Sequencing and Its Impact on Microbiome Analysis', 'display_name': 'Comparing Apples and Oranges?: Next Generation Sequencing and Its Impact on Microbiome Analysis', 'publication_year': 2016, 'publication_date': '2016-02-05', 'ids': {'openalex': 'https://openalex.org/W2260059978', 'doi': 'https://doi.org/10.1371/journal.pone.0148028', 'mag': '2260059978', 'pmid': 'https://pubmed.ncbi.nlm.nih.gov/26849217', 'pmcid': 'https://www.ncbi.nlm.nih.gov/pmc/articles/4746063'}, 'language': 'en', 'primary_location': {'is_oa': True, 'landing_page_url': 'https://doi.org/10.1371/journal.pone.0148028', 'pdf_url': 'https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0148028&type=printable', 'source': {'id': 'https://openalex.org/S202381698', 'display_name': 'PLoS ONE', 'issn_l': '1932-6203', 'issn': ['1932-6203'], 'is_oa': True, 'is_in_doaj': True, 'is_core': True, 'host_organization': 'https://openalex.org/P4310315706', 'host_organization_name': 'Public Library of Science', 'host_organization_lineage': ['https://openalex.org/P4310315706'], 'host_organization_lineage_names': ['Public Library of Science'], 'type': 'journal'}, 'license': 'public-domain', 'license_id': 'https://openalex.org/licenses/public-domain', 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, 'type': 'article', 'type_crossref': 'journal-article', 'indexed_in': ['crossref', 'pubmed'], 'open_access': {'is_oa': True, 'oa_status': 'gold', 'oa_url': 'https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0148028&type=printable', 'any_repository_has_fulltext': True}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5021685996', 'display_name': 'Adam G. Clooney', 'orcid': 'https://orcid.org/0000-0003-4881-9544'}, 'institutions': [{'id': 'https://openalex.org/I27577105', 'display_name': 'University College Cork', 'ror': 'https://ror.org/03265fv13', 'country_code': 'IE', 'type': 'education', 'lineage': ['https://openalex.org/I27577105']}, {'id': 'https://openalex.org/I4210100923', 'display_name': 'Munster Technological University', 'ror': 'https://ror.org/013xpqh61', 'country_code': 'IE', 'type': 'facility', 'lineage': ['https://openalex.org/I4210100923']}, {'id': 'https://openalex.org/I4210141138', 'display_name': 'APC Microbiome Institute', 'ror': 'https://ror.org/03f2ct267', 'country_code': 'IE', 'type': 'facility', 'lineage': ['https://openalex.org/I4210141138']}], 'countries': ['IE'], 'is_corresponding': True, 'raw_author_name': 'Adam G. Clooney', 'raw_affiliation_strings': ['APC Microbiome Institute, University College Cork, Cork, Ireland', 'Department of Biological Sciences Cork Institute of Technology, Cork, Ireland', 'School of Microbiology, University College Cork, Cork, Ireland'], 'affiliations': [{'raw_affiliation_string': 'APC Microbiome Institute, University College Cork, Cork, Ireland', 'institution_ids': ['https://openalex.org/I27577105', 'https://openalex.org/I4210141138']}, {'raw_affiliation_string': 'School of Microbiology, University College Cork, Cork, Ireland', 'institution_ids': ['https://openalex.org/I27577105']}, {'raw_affiliation_string': 'Department of Biological Sciences Cork Institute of Technology, Cork, Ireland', 'institution_ids': ['https://openalex.org/I4210100923']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5030391185', 'display_name': 'Fiona Fouhy', 'orcid': 'https://orcid.org/0000-0002-8285-7694'}, 'institutions': [{'id': 'https://openalex.org/I141766778', 'display_name': 'Teagasc - The Irish Agriculture and Food Development Authority', 'ror': 'https://ror.org/03sx84n71', 'country_code': 'IE', 'type': 'facility', 'lineage': ['https://openalex.org/I141766778']}, {'id': 'https://openalex.org/I4210141138', 'display_name': 'APC Microbiome Institute', 'ror': 'https://ror.org/03f2ct267', 'country_code': 'IE', 'type': 'facility', 'lineage': ['https://openalex.org/I4210141138']}, {'id': 'https://openalex.org/I27577105', 'display_name': 'University College Cork', 'ror': 'https://ror.org/03265fv13', 'country_code': 'IE', 'type': 'education', 'lineage': ['https://openalex.org/I27577105']}], 'countries': ['IE'], 'is_corresponding': True, 'raw_author_name': 'Fiona Fouhy', 'raw_affiliation_strings': ['APC Microbiome Institute, University College Cork, Cork, Ireland', 'Teagasc Food Research Centre, Moorepark, Fermoy, Ireland'], 'affiliations': [{'raw_affiliation_string': 'Teagasc Food Research Centre, Moorepark, Fermoy, Ireland', 'institution_ids': ['https://openalex.org/I141766778']}, {'raw_affiliation_string': 'APC Microbiome Institute, University College Cork, Cork, Ireland', 'institution_ids': ['https://openalex.org/I4210141138', 'https://openalex.org/I27577105']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5035931588', 'display_name': 'Roy D. Sleator', 'orcid': 'https://orcid.org/0000-0001-5846-3938'}, 'institutions': [{'id': 'https://openalex.org/I4210100923', 'display_name': 'Munster Technological University', 'ror': 'https://ror.org/013xpqh61', 'country_code': 'IE', 'type': 'facility', 'lineage': ['https://openalex.org/I4210100923']}, {'id': 'https://openalex.org/I4210141138', 'display_name': 'APC Microbiome Institute', 'ror': 'https://ror.org/03f2ct267', 'country_code': 'IE', 'type': 'facility', 'lineage': ['https://openalex.org/I4210141138']}, {'id': 'https://openalex.org/I27577105', 'display_name': 'University College Cork', 'ror': 'https://ror.org/03265fv13', 'country_code': 'IE', 'type': 'education', 'lineage': ['https://openalex.org/I27577105']}], 'countries': ['IE'], 'is_corresponding': True, 'raw_author_name': 'Roy D. Sleator', 'raw_affiliation_strings': ['APC Microbiome Institute, University College Cork, Cork, Ireland', 'Department of Biological Sciences Cork Institute of Technology, Cork, Ireland'], 'affiliations': [{'raw_affiliation_string': 'Department of Biological Sciences Cork Institute of Technology, Cork, Ireland', 'institution_ids': ['https://openalex.org/I4210100923']}, {'raw_affiliation_string': 'APC Microbiome Institute, University College Cork, Cork, Ireland', 'institution_ids': ['https://openalex.org/I4210141138', 'https://openalex.org/I27577105']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5068312119', 'display_name': 'Aisling O’Driscoll', 'orcid': 'https://orcid.org/0000-0001-8197-0911'}, 'institutions': [{'id': 'https://openalex.org/I4210100923', 'display_name': 'Munster Technological University', 'ror': 'https://ror.org/013xpqh61', 'country_code': 'IE', 'type': 'facility', 'lineage': ['https://openalex.org/I4210100923']}, {'id': 'https://openalex.org/I4210141138', 'display_name': 'APC Microbiome Institute', 'ror': 'https://ror.org/03f2ct267', 'country_code': 'IE', 'type': 'facility', 'lineage': ['https://openalex.org/I4210141138']}, {'id': 'https://openalex.org/I27577105', 'display_name': 'University College Cork', 'ror': 'https://ror.org/03265fv13', 'country_code': 'IE', 'type': 'education', 'lineage': ['https://openalex.org/I27577105']}], 'countries': ['IE'], 'is_corresponding': True, 'raw_author_name': 'Aisling O’ Driscoll', 'raw_affiliation_strings': ['APC Microbiome Institute, University College Cork, Cork, Ireland', 'Department of Computing, Cork Institute of Technology, Cork, Ireland'], 'affiliations': [{'raw_affiliation_string': 'Department of Computing, Cork Institute of Technology, Cork, Ireland', 'institution_ids': ['https://openalex.org/I4210100923']}, {'raw_affiliation_string': 'APC Microbiome Institute, University College Cork, Cork, Ireland', 'institution_ids': ['https://openalex.org/I4210141138', 'https://openalex.org/I27577105']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5074717784', 'display_name': 'Catherine Stanton', 'orcid': 'https://orcid.org/0000-0002-6724-7011'}, 'institutions': [{'id': 'https://openalex.org/I141766778', 'display_name': 'Teagasc - The Irish Agriculture and Food Development Authority', 'ror': 'https://ror.org/03sx84n71', 'country_code': 'IE', 'type': 'facility', 'lineage': ['https://openalex.org/I141766778']}, {'id': 'https://openalex.org/I4210141138', 'display_name': 'APC Microbiome Institute', 'ror': 'https://ror.org/03f2ct267', 'country_code': 'IE', 'type': 'facility', 'lineage': ['https://openalex.org/I4210141138']}, {'id': 'https://openalex.org/I27577105', 'display_name': 'University College Cork', 'ror': 'https://ror.org/03265fv13', 'country_code': 'IE', 'type': 'education', 'lineage': ['https://openalex.org/I27577105']}], 'countries': ['IE'], 'is_corresponding': True, 'raw_author_name': 'Catherine Stanton', 'raw_affiliation_strings': ['APC Microbiome Institute, University College Cork, Cork, Ireland', 'Teagasc Food Research Centre, Moorepark, Fermoy, Ireland'], 'affiliations': [{'raw_affiliation_string': 'Teagasc Food Research Centre, Moorepark, Fermoy, Ireland', 'institution_ids': ['https://openalex.org/I141766778']}, {'raw_affiliation_string': 'APC Microbiome Institute, University College Cork, Cork, Ireland', 'institution_ids': ['https://openalex.org/I4210141138', 'https://openalex.org/I27577105']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5050465932', 'display_name': 'Paul D. Cotter', 'orcid': 'https://orcid.org/0000-0002-5465-9068'}, 'institutions': [{'id': 'https://openalex.org/I141766778', 'display_name': 'Teagasc - The Irish Agriculture and Food Development Authority', 'ror': 'https://ror.org/03sx84n71', 'country_code': 'IE', 'type': 'facility', 'lineage': ['https://openalex.org/I141766778']}, {'id': 'https://openalex.org/I4210141138', 'display_name': 'APC Microbiome Institute', 'ror': 'https://ror.org/03f2ct267', 'country_code': 'IE', 'type': 'facility', 'lineage': ['https://openalex.org/I4210141138']}, {'id': 'https://openalex.org/I27577105', 'display_name': 'University College Cork', 'ror': 'https://ror.org/03265fv13', 'country_code': 'IE', 'type': 'education', 'lineage': ['https://openalex.org/I27577105']}], 'countries': ['IE'], 'is_corresponding': True, 'raw_author_name': 'Paul D. Cotter', 'raw_affiliation_strings': ['APC Microbiome Institute, University College Cork, Cork, Ireland', 'Teagasc Food Research Centre, Moorepark, Fermoy, Ireland'], 'affiliations': [{'raw_affiliation_string': 'Teagasc Food Research Centre, Moorepark, Fermoy, Ireland', 'institution_ids': ['https://openalex.org/I141766778']}, {'raw_affiliation_string': 'APC Microbiome Institute, University College Cork, Cork, Ireland', 'institution_ids': ['https://openalex.org/I4210141138', 'https://openalex.org/I27577105']}]}, {'author_position': 'last', 'author': {'id': 'https://openalex.org/A5061467408', 'display_name': 'Marcus J. Claesson', 'orcid': 'https://orcid.org/0000-0002-5712-0623'}, 'institutions': [{'id': 'https://openalex.org/I27577105', 'display_name': 'University College Cork', 'ror': 'https://ror.org/03265fv13', 'country_code': 'IE', 'type': 'education', 'lineage': ['https://openalex.org/I27577105']}, {'id': 'https://openalex.org/I4210141138', 'display_name': 'APC Microbiome Institute', 'ror': 'https://ror.org/03f2ct267', 'country_code': 'IE', 'type': 'facility', 'lineage': ['https://openalex.org/I4210141138']}], 'countries': ['IE'], 'is_corresponding': True, 'raw_author_name': 'Marcus J. Claesson', 'raw_affiliation_strings': ['APC Microbiome Institute, University College Cork, Cork, Ireland', 'School of Microbiology, University College Cork, Cork, Ireland'], 'affiliations': [{'raw_affiliation_string': 'School of Microbiology, University College Cork, Cork, Ireland', 'institution_ids': ['https://openalex.org/I27577105']}, {'raw_affiliation_string': 'APC Microbiome Institute, University College Cork, Cork, Ireland', 'institution_ids': ['https://openalex.org/I4210141138', 'https://openalex.org/I27577105']}]}], 'institution_assertions': [], 'countries_distinct_count': 1, 'institutions_distinct_count': 4, 'corresponding_author_ids': ['https://openalex.org/A5021685996', 'https://openalex.org/A5030391185', 'https://openalex.org/A5035931588', 'https://openalex.org/A5068312119', 'https://openalex.org/A5074717784', 'https://openalex.org/A5050465932', 'https://openalex.org/A5061467408'], 'corresponding_institution_ids': ['https://openalex.org/I27577105', 'https://openalex.org/I4210100923', 'https://openalex.org/I4210141138', 'https://openalex.org/I141766778', 'https://openalex.org/I4210141138', 'https://openalex.org/I27577105', 'https://openalex.org/I4210100923', 'https://openalex.org/I4210141138', 'https://openalex.org/I27577105', 'https://openalex.org/I4210100923', 'https://openalex.org/I4210141138', 'https://openalex.org/I27577105', 'https://openalex.org/I141766778', 'https://openalex.org/I4210141138', 'https://openalex.org/I27577105', 'https://openalex.org/I141766778', 'https://openalex.org/I4210141138', 'https://openalex.org/I27577105', 'https://openalex.org/I27577105', 'https://openalex.org/I4210141138'], 'apc_list': {'value': 1805, 'currency': 'USD', 'value_usd': 1805, 'provenance': 'doaj'}, 'apc_paid': {'value': 1805, 'currency': 'USD', 'value_usd': 1805, 'provenance': 'doaj'}, 'fwci': 13.184, 'has_fulltext': True, 'fulltext_origin': 'pdf', 'cited_by_count': 235, 'citation_normalized_percentile': {'value': 0.999985, 'is_in_top_1_percent': True, 'is_in_top_10_percent': True}, 'cited_by_percentile_year': {'min': 99, 'max': 100}, 'biblio': {'volume': '11', 'issue': '2', 'first_page': 'e0148028', 'last_page': 'e0148028'}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T10066', 'display_name': 'Diversity and Function of Gut Microbiome', 'score': 0.999, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, 'topics': [{'id': 'https://openalex.org/T10066', 'display_name': 'Diversity and Function of Gut Microbiome', 'score': 0.999, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T10015', 'display_name': 'RNA Sequencing Data Analysis', 'score': 0.9974, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T11791', 'display_name': 'Marine Microbial Diversity and Biogeography', 'score': 0.9871, 'subfield': {'id': 'https://openalex.org/subfields/2303', 'display_name': 'Ecology'}, 'field': {'id': 'https://openalex.org/fields/23', 'display_name': 'Environmental Science'}, 'domain': {'id': 'https://openalex.org/domains/3', 'display_name': 'Physical Sciences'}}], 'keywords': [{'id': 'https://openalex.org/keywords/amplicon-sequencing', 'display_name': 'Amplicon sequencing', 'score': 0.75402087}, {'id': 'https://openalex.org/keywords/amplicon', 'display_name': 'Amplicon', 'score': 0.75333}, {'id': 'https://openalex.org/keywords/sequence-alignment', 'display_name': 'sequence alignment', 'score': 0.553585}, {'id': 'https://openalex.org/keywords/ion-semiconductor-sequencing', 'display_name': 'Ion semiconductor sequencing', 'score': 0.5452025}, {'id': 'https://openalex.org/keywords/sequence-variation', 'display_name': 'sequence variation', 'score': 0.531066}, {'id': 'https://openalex.org/keywords/metagenomic-analysis', 'display_name': 'Metagenomic Analysis', 'score': 0.521871}, {'id': 'https://openalex.org/keywords/massive-parallel-sequencing', 'display_name': 'Massive parallel sequencing', 'score': 0.43994355}], 'concepts': [{'id': 'https://openalex.org/C101985253', 'wikidata': 'https://www.wikidata.org/wiki/Q1073526', 'display_name': 'Shotgun sequencing', 'level': 4, 'score': 0.77056223}, {'id': 'https://openalex.org/C2993206177', 'wikidata': 'https://www.wikidata.org/wiki/Q2844457', 'display_name': 'Amplicon sequencing', 'level': 4, 'score': 0.75402087}, {'id': 'https://openalex.org/C8185291', 'wikidata': 'https://www.wikidata.org/wiki/Q2844457', 'display_name': 'Amplicon', 'level': 4, 'score': 0.75333}, {'id': 'https://openalex.org/C86803240', 'wikidata': 'https://www.wikidata.org/wiki/Q420', 'display_name': 'Biology', 'level': 0, 'score': 0.6613712}, {'id': 'https://openalex.org/C143121216', 'wikidata': 'https://www.wikidata.org/wiki/Q1330402', 'display_name': 'Microbiome', 'level': 2, 'score': 0.59892535}, {'id': 'https://openalex.org/C15151743', 'wikidata': 'https://www.wikidata.org/wiki/Q903778', 'display_name': 'Metagenomics', 'level': 3, 'score': 0.5989208}, {'id': 'https://openalex.org/C51679486', 'wikidata': 'https://www.wikidata.org/wiki/Q380546', 'display_name': 'DNA sequencing', 'level': 3, 'score': 0.5868281}, {'id': 'https://openalex.org/C132917006', 'wikidata': 'https://www.wikidata.org/wiki/Q5250436', 'display_name': 'Deep sequencing', 'level': 4, 'score': 0.57639503}, {'id': 'https://openalex.org/C70721500', 'wikidata': 'https://www.wikidata.org/wiki/Q177005', 'display_name': 'Computational biology', 'level': 1, 'score': 0.5465973}, {'id': 'https://openalex.org/C164928082', 'wikidata': 'https://www.wikidata.org/wiki/Q6063435', 'display_name': 'Ion semiconductor sequencing', 'level': 4, 'score': 0.5452025}, {'id': 'https://openalex.org/C54355233', 'wikidata': 'https://www.wikidata.org/wiki/Q7162', 'display_name': 'Genetics', 'level': 1, 'score': 0.44289225}, {'id': 'https://openalex.org/C192800701', 'wikidata': 'https://www.wikidata.org/wiki/Q6784807', 'display_name': 'Massive parallel sequencing', 'level': 4, 'score': 0.43994355}, {'id': 'https://openalex.org/C49105822', 'wikidata': 'https://www.wikidata.org/wiki/Q176996', 'display_name': 'Polymerase chain reaction', 'level': 3, 'score': 0.2951224}, {'id': 'https://openalex.org/C42062724', 'wikidata': 'https://www.wikidata.org/wiki/Q1209205', 'display_name': '16S ribosomal RNA', 'level': 3, 'score': 0.1972478}, {'id': 'https://openalex.org/C141231307', 'wikidata': 'https://www.wikidata.org/wiki/Q7020', 'display_name': 'Genome', 'level': 3, 'score': 0.14690247}, {'id': 'https://openalex.org/C104317684', 'wikidata': 'https://www.wikidata.org/wiki/Q7187', 'display_name': 'Gene', 'level': 2, 'score': 0.12977612}], 'mesh': [{'descriptor_ui': 'D059014', 'descriptor_name': 'High-Throughput Nucleotide Sequencing', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': True}, {'descriptor_ui': 'D056186', 'descriptor_name': 'Metagenomics', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': True}, {'descriptor_ui': 'D064307', 'descriptor_name': 'Microbiota', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': True}, {'descriptor_ui': 'D000368', 'descriptor_name': 'Aged', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D017931', 'descriptor_name': 'DNA Primers', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': False}, {'descriptor_ui': 'D017931', 'descriptor_name': 'DNA Primers', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D005243', 'descriptor_name': 'Feces', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D005243', 'descriptor_name': 'Feces', 'qualifier_ui': 'Q000382', 'qualifier_name': 'microbiology', 'is_major_topic': False}, {'descriptor_ui': 'D006801', 'descriptor_name': 'Humans', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D064307', 'descriptor_name': 'Microbiota', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D012336', 'descriptor_name': 'RNA, Ribosomal, 16S', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D012336', 'descriptor_name': 'RNA, Ribosomal, 16S', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': False}, {'descriptor_ui': 'D017423', 'descriptor_name': 'Sequence Analysis, RNA', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}], 'locations_count': 7, 'locations': [{'is_oa': True, 'landing_page_url': 'https://doi.org/10.1371/journal.pone.0148028', 'pdf_url': 'https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0148028&type=printable', 'source': {'id': 'https://openalex.org/S202381698', 'display_name': 'PLoS ONE', 'issn_l': '1932-6203', 'issn': ['1932-6203'], 'is_oa': True, 'is_in_doaj': True, 'is_core': True, 'host_organization': 'https://openalex.org/P4310315706', 'host_organization_name': 'Public Library of Science', 'host_organization_lineage': ['https://openalex.org/P4310315706'], 'host_organization_lineage_names': ['Public Library of Science'], 'type': 'journal'}, 'license': 'public-domain', 'license_id': 'https://openalex.org/licenses/public-domain', 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, {'is_oa': True, 'landing_page_url': 'http://hdl.handle.net/11019/987', 'pdf_url': 'https://t-stor.teagasc.ie/bitstream/11019/987/1/e0148028.pdf', 'source': {'id': 'https://openalex.org/S4306402356', 'display_name': 'T-Stór (Teagasc)', 'issn_l': None, 'issn': None, 'is_oa': True, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I141766778', 'host_organization_name': 'Teagasc - The Irish Agriculture and Food Development Authority', 'host_organization_lineage': ['https://openalex.org/I141766778'], 'host_organization_lineage_names': ['Teagasc - The Irish Agriculture and Food Development Authority'], 'type': 'repository'}, 'license': 'cc-by-nc-sa', 'license_id': 'https://openalex.org/licenses/cc-by-nc-sa', 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, {'is_oa': True, 'landing_page_url': 'http://hdl.handle.net/10468/3654', 'pdf_url': 'https://cora.ucc.ie/bitstreams/6869ef63-5e7e-439c-a298-af6b15519759/download', 'source': {'id': 'https://openalex.org/S4306400766', 'display_name': 'Cork Open Research Archive (University College Cork)', 'issn_l': None, 'issn': None, 'is_oa': True, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I27577105', 'host_organization_name': 'University College Cork', 'host_organization_lineage': ['https://openalex.org/I27577105'], 'host_organization_lineage_names': ['University College Cork'], 'type': 'repository'}, 'license': 'public-domain', 'license_id': 'https://openalex.org/licenses/public-domain', 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, {'is_oa': True, 'landing_page_url': 'https://europepmc.org/articles/pmc4746063', 'pdf_url': 'https://europepmc.org/articles/pmc4746063?pdf=render', 'source': {'id': 'https://openalex.org/S4306400806', 'display_name': 'Europe PMC (PubMed Central)', 'issn_l': None, 'issn': None, 'is_oa': True, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1303153112', 'host_organization_name': 'European Bioinformatics Institute', 'host_organization_lineage': ['https://openalex.org/I1303153112'], 'host_organization_lineage_names': ['European Bioinformatics Institute'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, {'is_oa': True, 'landing_page_url': 'https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4746063', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S2764455111', 'display_name': 'PubMed Central', 'issn_l': None, 'issn': None, 'is_oa': True, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, {'is_oa': True, 'landing_page_url': 'https://figshare.com/articles/dataset/Comparing_Apples_and_Oranges_Next_Generation_Sequencing_and_Its_Impact_on_Microbiome_Analysis/2596813', 'pdf_url': 'https://figshare.com/ndownloader/files/4244635', 'source': {'id': 'https://openalex.org/S4306402621', 'display_name': 'INDIGO (University of Illinois at Chicago)', 'issn_l': None, 'issn': None, 'is_oa': True, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I39422238', 'host_organization_name': 'University of Illinois at Chicago', 'host_organization_lineage': ['https://openalex.org/I39422238'], 'host_organization_lineage_names': ['University of Illinois at Chicago'], 'type': 'repository'}, 'license': 'cc-by', 'license_id': 'https://openalex.org/licenses/cc-by', 'version': 'submittedVersion', 'is_accepted': False, 'is_published': False}, {'is_oa': False, 'landing_page_url': 'https://pubmed.ncbi.nlm.nih.gov/26849217', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4306525036', 'display_name': 'PubMed', 'issn_l': None, 'issn': None, 'is_oa': False, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}], 'best_oa_location': {'is_oa': True, 'landing_page_url': 'https://doi.org/10.1371/journal.pone.0148028', 'pdf_url': 'https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0148028&type=printable', 'source': {'id': 'https://openalex.org/S202381698', 'display_name': 'PLoS ONE', 'issn_l': '1932-6203', 'issn': ['1932-6203'], 'is_oa': True, 'is_in_doaj': True, 'is_core': True, 'host_organization': 'https://openalex.org/P4310315706', 'host_organization_name': 'Public Library of Science', 'host_organization_lineage': ['https://openalex.org/P4310315706'], 'host_organization_lineage_names': ['Public Library of Science'], 'type': 'journal'}, 'license': 'public-domain', 'license_id': 'https://openalex.org/licenses/public-domain', 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, 'sustainable_development_goals': [{'id': 'https://metadata.un.org/sdg/10', 'score': 0.59, 'display_name': 'Reduced inequalities'}], 'grants': [], 'datasets': [], 'versions': [], 'referenced_works_count': 38, 'referenced_works': ['https://openalex.org/W122717592', 'https://openalex.org/W1512978277', 'https://openalex.org/W1847253990', 'https://openalex.org/W2000040814', 'https://openalex.org/W2012854889', 'https://openalex.org/W2015814010', 'https://openalex.org/W2025583379', 'https://openalex.org/W2028286172', 'https://openalex.org/W2030353410', 'https://openalex.org/W2037279825', 'https://openalex.org/W2047669659', 'https://openalex.org/W2048673910', 'https://openalex.org/W2066194720', 'https://openalex.org/W2072698534', 'https://openalex.org/W2077407690', 'https://openalex.org/W2085284704', 'https://openalex.org/W2088170171', 'https://openalex.org/W2094100452', 'https://openalex.org/W2094492452', 'https://openalex.org/W2095132285', 'https://openalex.org/W2108718991', 'https://openalex.org/W2109848222', 'https://openalex.org/W2117457769', 'https://openalex.org/W2117654562', 'https://openalex.org/W2119284644', 'https://openalex.org/W2120501335', 'https://openalex.org/W2124715997', 'https://openalex.org/W2127175247', 'https://openalex.org/W2131026689', 'https://openalex.org/W2131271579', 'https://openalex.org/W2136879569', 'https://openalex.org/W2137123184', 'https://openalex.org/W2139701562', 'https://openalex.org/W2141920662', 'https://openalex.org/W2145025818', 'https://openalex.org/W2149573313', 'https://openalex.org/W2159954944', 'https://openalex.org/W2166171121'], 'related_works': ['https://openalex.org/W4379373911', 'https://openalex.org/W4311309808', 'https://openalex.org/W2951076599', 'https://openalex.org/W2921468924', 'https://openalex.org/W2905479625', 'https://openalex.org/W2884918195', 'https://openalex.org/W2756362175', 'https://openalex.org/W2613050520', 'https://openalex.org/W2409976444', 'https://openalex.org/W2260059978'], 'abstract_inverted_index': {'Rapid': [0], 'advancements': [1], 'in': [2, 34, 62, 136, 158, 271, 298], 'sequencing': [3, 47, 88, 94, 117, 162, 210, 237, 244], 'technologies': [4], 'along': [5], 'with': [6, 185, 204, 283, 295], 'falling': [7], 'costs': [8], 'present': [9], 'widespread': [10], 'opportunities': [11], 'for': [12, 89, 132, 195, 220, 225, 242, 259], 'microbiome': [13, 159], 'studies': [14, 74], 'across': [15, 118], 'a': [16, 31, 167, 256], 'vast': [17], 'and': [18, 55, 70, 86, 108, 170, 212, 235], 'diverse': [19], 'array': [20], 'of': [21, 37, 73, 153, 191, 201, 214, 245], 'environments.': [22], 'These': [23, 96], 'impressive': [24], 'technological': [25], 'developments': [26], 'have': [27], 'been': [28], 'accompanied': [29], 'by': [30], 'considerable': [32], 'growth': [33], 'the': [35, 68, 71, 79, 90, 129, 133, 140, 145, 154, 177, 189, 221, 232, 252, 280], 'number': [36], 'methodological': [38, 275], 'variables,': [39], 'including': [40], 'sampling,': [41], 'storage,': [42], 'DNA': [43], 'extraction,': [44], 'primer': [45], 'pairs,': [46], 'technology,': [48], 'chemistry': [49], 'version,': [50], 'read': [51, 268], 'length,': [52], 'insert': [53], 'size,': [54], 'analysis': [56], 'pipelines,': [57], 'amongst': [58], 'others.': [59], 'This': [60], 'increase': [61], 'variability': [63], 'threatens': [64], 'to': [65, 99, 166, 199, 265, 273, 305], 'compromise': [66], 'both': [67, 84], 'reproducibility': [69], 'comparability': [72], 'conducted.': [75], 'Here': [76], 'we': [77, 126], 'perform': [78], 'first': [80], 'reported': [81], 'study': [82, 278], 'comparing': [83, 284], 'amplicon': [85, 116], 'shotgun': [87, 111, 196, 243], 'three': [91], 'leading': [92], 'next-generation': [93], 'technologies.': [95], 'were': [97, 183], 'applied': [98, 233], 'six': [100], 'human': [101], 'stool': [102, 246], 'samples': [103], 'using': [104, 287, 310], 'Illumina': [105], 'HiSeq,': [106], 'MiSeq': [107, 234], 'Ion': [109], 'PGM': [110, 236], 'sequencing,': [112], 'as': [113, 115], 'well': [114], 'two': [119], 'variable': [120], '16S': [121, 178], 'rRNA': [122, 179], 'gene': [123, 261], 'regions.': [124], 'Notably,': [125], 'found': [127], 'that': [128, 293], 'factor': [130], 'responsible': [131], 'greatest': [134], 'variance': [135], 'microbiota': [137], 'composition': [138], 'was': [139, 173, 223], 'chosen': [141], 'methodology': [142], 'rather': [143], 'than': [144, 209], 'natural': [146], 'inter-individual': [147], 'variance,': [148], 'which': [149], 'is': [150], 'commonly': [151], 'one': [152], 'most': [155], 'significant': [156], 'drivers': [157], 'studies.': [160], 'Amplicon': [161], 'suffered': [163], 'from': [164], 'this': [165, 171, 277], 'large': [168], 'extent,': [169], 'issue': [172], 'particularly': [174], 'apparent': [175], 'when': [176], 'V1-V2': [180], 'region': [181], 'amplicons': [182], 'sequenced': [184], 'MiSeq.': [186], 'Somewhat': [187], 'surprisingly,': [188], 'choice': [190, 213], 'taxonomic': [192], 'binning': [193], 'software': [194], 'sequences': [197], 'proved': [198, 239], 'be': [200], 'crucial': [202], 'importance': [203], 'even': [205], 'greater': [206], 'discriminatory': [207], 'power': [208], 'technology': [211], 'amplicon.': [215], 'Optimal': [216], 'N50': [217], 'assembly': [218], 'values': [219], 'HiSeq': [222], 'obtained': [224], '10': [226], 'million': [227], 'reads': [228], 'per': [229], 'sample,': [230], 'whereas': [231], 'depths': [238], 'less': [240], 'sufficient': [241], 'samples.': [247], 'The': [248], 'latter': [249], 'technologies,': [250], 'on': [251], 'other': [253], 'hand,': [254], 'provide': [255], 'better': [257], 'basis': [258], 'functional': [260], 'categorisation,': [262], 'possibly': [263], 'due': [264], 'their': [266, 303], 'longer': [267], 'lengths.': [269], 'Hence,': [270], 'addition': [272], 'highlighting': [274], 'biases,': [276], 'demonstrates': [279], 'risks': [281], 'associated': [282], 'data': [285], 'generated': [286], 'different': [288, 311], 'strategies.': [289], 'We': [290], 'also': [291], 'recommend': [292], 'laboratories': [294], 'particular': [296], 'interests': [297], 'certain': [299], 'microbes': [300], 'should': [301], 'optimise': [302], 'protocols': [304], 'accurately': [306], 'detect': [307], 'these': [308], 'taxa': [309], 'techniques.': [312]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2260059978', 'counts_by_year': [{'year': 2024, 'cited_by_count': 11}, {'year': 2023, 'cited_by_count': 25}, {'year': 2022, 'cited_by_count': 19}, {'year': 2021, 'cited_by_count': 37}, {'year': 2020, 'cited_by_count': 34}, {'year': 2019, 'cited_by_count': 39}, {'year': 2018, 'cited_by_count': 36}, {'year': 2017, 'cited_by_count': 22}, {'year': 2016, 'cited_by_count': 10}], 'updated_date': '2024-09-17T17:14:10.877830', 'created_date': '2016-06-24'}