Title: Effect of artesunate on osmotic fragility of red blood cells.
Abstract: Objective To research the effect of artesunate on the osmotic fragility of red blood cells and the clinical using safety of artesunate.Methods Different concentrations of artesunate were incubated with red blood cells for 12 h,the levels of hemolysis were detecte by visual observation and hemiglobincyanide;the red blood cell osmotic fragility test was used to detected the maximum and minimum osmotic fragility of red blood cells in each group treated by different concentration of artesunate.The hemolysis status of maximum osmotic fragility in every group was detected by the hemiglobincyanide.The statistics software SPSS16.0 was applied to compare the results of different groups.Results No significant hemolysis of red blood cells incubated with artsunate was observed by eyes,there were no conspicuous differences among the optical density of every group treated by artsunate when analyzed by the ANOVA(P0.05).Except two low concentration groups(the final concentration of artesunate was 12.5,6.25 μg/mL),the maximum and minimum osmotic fragilities of red blood cells in the remaining groups were higher than those of normal or the control group.With the drug concentration of the maximum osmotic fragility increased,the degree of hemolysis of the maximum osmotic fragility also increased when detected by the visual observation or the hemiglobincyanide.When the concentration of artesunate ≥ 25 μg/mL,the optical density of maximum osmotic fragility increased gradually,there was significant difference(P0.05) between each drug treated group and the control group.But when the concentration of artesunate ≤12.5 μg/mL,there was no difference between the experimental group and the control one(P0.05).Conclusion The current clinical dose of artesunate is relatively safe,though the high-dose of artesunate can not directly cause the hemolysis of red blood cells,but it can increase the osmotic fragility of RBC,the intravascular hemolysis must be vigilant when increasing dosage.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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