Title: Production of specific antibodies against protein A fusion proteins.
Abstract: Research Article1 September 1986free access Production of specific antibodies against protein A fusion proteins. B. Löwenadler B. Löwenadler Search for more papers by this author B. Nilsson B. Nilsson Search for more papers by this author L. Abrahmsén L. Abrahmsén Search for more papers by this author T. Moks T. Moks Search for more papers by this author L. Ljungqvist L. Ljungqvist Search for more papers by this author E. Holmgren E. Holmgren Search for more papers by this author S. Paleus S. Paleus Search for more papers by this author S. Josephson S. Josephson Search for more papers by this author L. Philipson L. Philipson Search for more papers by this author M. Uhlén M. Uhlén Search for more papers by this author B. Löwenadler B. Löwenadler Search for more papers by this author B. Nilsson B. Nilsson Search for more papers by this author L. Abrahmsén L. Abrahmsén Search for more papers by this author T. Moks T. Moks Search for more papers by this author L. Ljungqvist L. Ljungqvist Search for more papers by this author E. Holmgren E. Holmgren Search for more papers by this author S. Paleus S. Paleus Search for more papers by this author S. Josephson S. Josephson Search for more papers by this author L. Philipson L. Philipson Search for more papers by this author M. Uhlén M. Uhlén Search for more papers by this author Author Information B. Löwenadler, B. Nilsson, L. Abrahmsén, T. Moks, L. Ljungqvist, E. Holmgren, S. Paleus, S. Josephson, L. Philipson and M. Uhlén The EMBO Journal (1986)5:2393-2398https://doi.org/10.1002/j.1460-2075.1986.tb04509.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info The gene for Staphylococcal protein A was fused to the coding sequence of bacterial beta-galactosidase, alkaline phosphatase and human insulin-like growth factor I (IGF-I). The fusion proteins, expressed in bacteria, were purified by affinity chromatography on IgG-Sepharose and antibodies were raised in rabbits. All three fusion proteins elicited specific antibodies against both the inserted protein sequences and the protein A moiety. In the case of IGF-I, the protein A moiety in the fusion protein may act as an adjuvant since native IGF-I alone is a poor immunogen. The results suggest that the protein A fusion system can be used for efficient antibody production against peptides or proteins expressed from cloned or synthetic genes. To facilitate such gene fusions a set of optimized vectors have been constructed. Previous ArticleNext Article Volume 5Issue 91 September 1986In this issue RelatedDetailsLoading ...