Title: THE EFFECTS OF TIME, TEMPERATURE, SOLVENT: SOLID RATIO AND SOLVENT COMPOSITION ON EXTRACTION OF TOTAL PHENOLIC COMPOUND FROM DRIED OLIVE (Olea europaea L.) LEAVES
Abstract: The optimum extraction conditions for total phenolic compounds from dried olive leaves were determined by using response surface methodology. Central composite design was used to investigate the effects of four independent variables such as solvent composition (ethanol in water, 20 to 100%), extraction temperature (20 to 60 °C), extraction time (4-48 hours) and solvent/solid ratio (4 to 8). As a response total phenolic compound content of the extracts were chosen. The quadratic model is used for predicting the results and R 2 was found 0.8539. The olive leaves with the initial 43.94±0.25% moisture content were dried in vacuum oven at 60 oC to 2±0.07% moisture content before extraction. There are several solutions by choosing the target, in range, maximize or minimize the effective parameters in response surface methodology. In this study, recommended optimal conditions for the total phenolic compounds from olive leaves were found 43% ethanol in water (v/v), 50 oC, 15 hours and 7 times solvent/solid ratio. Under the chosen optimum conditions the corresponding predicted response value for total phenolic compounds was 4586.3 mg GAE/100 g dried leaves.
Publication Year: 2010
Publication Date: 2010-12-01
Language: en
Type: article
Access and Citation
Cited By Count: 14
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot