Title: In Vitro Clonal Propagation of Guava (Psidium guajava, L.)
Abstract: A method of micropropagatlon through in vitro shoot bud culture has been standardized in guava cv. Pant Prabhat. The stock plant was maintained at the field in open condition and pruned severally to encourage new growth. Around 3 cm long newly emerged shoots were taken as explant. These explants were collected in a solution of 100 ml containing 0.1% Bavistin, 20 mg PVP, 1.0 mg streptomycin and 2 ml Twin-20 and then kept under running tap water for 1 hour. The pretreated explants were further treated with various sterilizing agents followed by 4–5 washing with autoclaved distilled water. The best aseptic culture as well as survival of explant was obtained when explants were treated with mercuric chloride (O.l %) for 5 minutes and sodium hypochloride (1%) for 8 minutes In sequential order. Addition of 500 mg citric acid, an oxidant to the MS media and initial incubation of cultures In complete dark for 24 hours showed substantial reduction in phenolic browning of media and higher survival of explants. Transferrring of explants of MS media containing 4.0 mg/L BA and 0.2 mg/L IBA resulted in higher shoot proliferation, however, higher shoot growth was recorded with 3.0 mg/L BA and 0.2 mg/L IBA. Rooting was induced in half-strength MS medium supplemented with 0.4 mg/L IBA and 0.2 mg/L NAA.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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Cited By Count: 2
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