Title: Recovery and fertilization rates of goat spermatozoa as affected by different levels of egg yolk, dilution rates, freezing method and months of the year.
Abstract: ABSTRACTSemen from10 fertile 1/2 Damascus-1/2 Baladi crossbred bucks (3-5 years old)was collected by artificial vagina twice weekly for 12 months. Ejaculates with massmotility ≥80% were pooled to be used in series of five experiments. In the 1stexperiment, semen was diluted with Tris-citric acid-glucose-glycerol extender to selectthe best level of egg yolk among the levels 2.5, 5.0, 7.5, 10.0, 15.0 and 20% verifyingthe highest sperm post-thawing motility where it was found as 2.5%. Experiment II,was to select the best dilution rate among the rates 1:4, 1:5, 1:8, 1:10 and 1:16 whichwas found to be 1:5. Semen diluted with 2.5% egg yolk in Tris-based extender at a rateof 1:5 was used to compare freezing in straws or in pellets form (Exp. III). Inexperiment IV, post-thaw sperm motility and recovery rate were compared amongmonths of the year. Fertility rate by using frozen semen in pellets form with differentsperm cell doses (100, 200 and 300x106/cervical inseminate) was assessed (Exp. V).The results revealed that sperm motility percentage was the highest in postthawsemen diluted with Tris extender containing 2.5% egg yolk at a rate of 1: 5(54.5%, P<0.05) and the lowest (45.4%, P<0.05) at a rate of 1:16. Freezing goat semenshowed higher post-thaw motility when was in pellets compared to straws (49.04% vs.43.54%, P<0.05). Sperm motility in fresh, post-diluted and post-thaw semen showedthe highest values during autumn months (Sept.-Nov.) and the lowest during summer
months (June-Aug.). Fertility rate in does artificially inseminated with 100, 200 and 300x 106 motile spermatozoa in pellets form was 31.57, 42.10 and 50.00%, respectively.
Publication Year: 2010
Publication Date: 2010-04-01
Language: en
Type: article
Access and Citation
Cited By Count: 6
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot