Title: Effect of medium composition and fermentation conditions on α-galactosidase production by Aspergillus niger v. Tiegh.
Abstract: One-factor-at-a-time method was applied to investigate the medium composition and fermentation conditions for α-galactosidase production by a mutant strain Aspergillus niger v.Tiegh CGMCC1182 in solid-state fermentation,and then orthogonal Ln18(37) design was used to further optimize the seven significant factors.The optimal medium was composed of wheat bran(WB) and soybean meal(SM) with a ratio of 7∶3(W/W),2.0% of raffinose,2.5% of(NH4)2SO4 and 0.5% of MgSO4.The optimum temperature and moisture level for α-galactosidase production were 30.0 ℃ and 55%,respectively.Fourteen gram of medium with 10%(V/W) of inoculum(107 spores·mL-1) in a 250 mL flask was suitable for enzyme production,and the maximum α-galactosidase activity reached 308.5 U·g-1 at a period of 68 h cultivation.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot