Title: Correlation of salinity on previtellogenic development of female Pacific white shrimp (Litopenaeus vannamei Boone, 1931).
Abstract: Successful induced maturation of female shrimp consists of three stages of gonadal development, namely, previtellogensis, vitellogensis, and final maturation. Previous research in shrimp reproduction investigated mostly the final maturation stage and spawning process, and few studies were conducted on previtellogenic ovarian development. Several factors influence previtellogenesis of female Pacific white shrimp (Litopenaeus vannamei Boone, 1931). In this study, the effect of salinity, one of the most powerful factors, was investigated. Female L. vannamei, (45 g body weight, 120 days old) with previtellogenic ovaries were reared in cages (5x5 m 2 ) installed in two earthen ponds. Salinity levels were 15 and 30 ppt. Haemolymph, hepatopancreas and ovaries of the experimental shrimp were collected monthly for hormone analysis. P4, E2 and PGF 2a levels in haemolymph of the shrimp samples were measured by either RP-HPLC or RIA. Biological marker (Primer) specific for 357 by of Vg and 314 by of FAMeT gene modified from NCBI were developed from the hepatopancreas of female L. vannamei. Vg and FAMeT gene expression levels using l st cDNA synthesis from hepatopancreas and ovary tissues were determined by RT-PCR. Results showed that the levels of Vg and FAMeT gene expression in the ovaries and hepatopancreas of shrimp reared in 30 ppt were significantly higher than those of shrimp reared in 15 ppt (P<0.05). The mean expression level of Vg and FAMeT in shrimp hepatopancreas was higher than in the ovaries. Similarly, changes in P4, E2 and PGF 2a concentrations in haemolymph and GSI of shrimp reared in 30 ppt were significantly higher than those of shrimp reared in 15 ppt (P<0.05). In conclusion, the experiment showed that salinity played an important role in shrimp ovarian development at different levels of changes, namely morphological (GSI), hormonal and gene expression levels.
Publication Year: 2015
Publication Date: 2015-01-01
Language: en
Type: article
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