Title: BMR Biochemistry-Production of L-asparaginase By Solid State Fermentation Using Marine Fungus
Abstract: Beauveria bassiana (MSS18/41) was used in the present study for L-asparaginase production, as an extracellular enzyme by solid state fermentation (SSF). Initially Beauveria bassiana was evaluated for its ability to produce L-asparaginase by rapid plate assay. Various fermentation parameters such as types of agro material as carbon source, particle size, solid substrate concentration, inoculum level, moisture content, temperature and incubation period which influence the rate of enzyme production under SSF were optimized. Different substrates like rice husk, wheat bran, coconut oilcake, black gram husk, green gram husk were used as carbon source and fermentation studies were carried out separately for each agro material. The maximum yield of L-asparaginase (90U/gds) was achieved with the following optimized fermentation parameters: Solid substrate (wheat bran) concentration (10gms), particle size (3mm), inoculums concentration (20%), moisture content (50%v/w), alkaline pH 9.0, temperature (26oC), and incubation period (96 hrs).These results indicate that the marine fungal Beauveria bassiana strain (MSS18/41 ) utilized wheat bran for the production of L-asparaginase.
Publication Year: 2014
Publication Date: 2014-06-10
Language: en
Type: article
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Cited By Count: 1
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