Title: The effect of normalization, CT attenuation, and scatter corrections on quantitative rat brain imaging with FDG-PET
Abstract:2643 Objectives Identify the effects of normalization, CT attenuation, and scatter correction on quantitative analysis of FDG uptake in the rat brain. Methods FDG uptake was measured in adult male Spr...2643 Objectives Identify the effects of normalization, CT attenuation, and scatter correction on quantitative analysis of FDG uptake in the rat brain. Methods FDG uptake was measured in adult male Sprague-Dawley (SD) rats (N=24) using a Siemens Inveon PET/CT scanner. FDG (1.5-2.0 mCi) was injected via the tail vein of anesthetized rats, which were maintained under anesthesia during uptake and imaging. 45 minutes after uptake PET scan was acquired in list mode for 30 minutes. Prior to PET scan a CT scan was acquired and used for anatomical localization and attenuation correction. PET data was reconstructed with 1) both attenuation and scatter corrections (AC/SC), 2) attenuation correction only (AC), and 3) no corrections (NO). Cuboid regions of interest (ROI) were assessed in the midbrain and cerebellum using Inveon Research Workplace (IRW) software. The midbrain region was normalized using the standard uptake value (SUV) and cerebellum as reference region. Results Significant differences in activity concentration using AC/SC, AC and NO were demonstrated with a one-way ANOVA. Reference tissue normalization (cerebellum) provides improvement in precision as compared to SUV normalization or no normalization (Table). Precision of approximately 5% is demonstrated for reference tissue normalization. Conclusions Normalization significantly improved precision and minimized differences due to attenuation and scatter corrections for quantitative measurements. Use of the cerebellum as a reference region may facilitate detection of small changes in the rat brain by FDG PET. Research Support The study was supported by the Center for Neuroscience and Regenerative Medicine.Read More
Publication Year: 2014
Publication Date: 2014-05-01
Language: en
Type: article
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