Title: Abstract 2039: Trim21: A novel regulator of Par-4 and oncogenic signaling
Abstract: Abstract Prostate apoptosis response protein 4 (Par-4) is a tumor suppressor that induces apoptosis selectively in cancer cells. Although Par-4 levels have been shown to be regulated by ubiqutin-mediated proteolysis, it is not known whether Par-4 function is regulated by ubiquination. To identify novel protein interaction partners of Par-4, we used co-immunoprecipitation of Par-4, followed by in-solution trypsin digestion and mass spectrometry. We demonstrate that Trim21, an E3 ligase that acts as a negative regulator of inflammatory signaling partially through ubiquitination of various downstream transcription factors, is a novel Par-4 interacting protein, and may regulate the activity of Par-4. Furthermore, similar to Par-4, we found that Trim21 inhibits Akt activation. Our studies reveal that Trim21 is a novel E3 ligase of Par-4, and extends its role from regulation of immune signaling to regulation of apoptosis and oncogenic signaling in colon cancer cells. Citation Format: Jeffrey Nguyen, Rosalyn Irby. Trim21: A novel regulator of Par-4 and oncogenic signaling. [abstract]. In: Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18-22; Philadelphia, PA. Philadelphia (PA): AACR; Cancer Res 2015;75(15 Suppl):Abstract nr 2039. doi:10.1158/1538-7445.AM2015-2039
Publication Year: 2015
Publication Date: 2015-08-01
Language: en
Type: article
Indexed In: ['crossref']
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