Title: [132] Fluorometric analyses of riboflavin and its coenzymes
Abstract: The most widespread flavins of biological importance are riboflavin (Rb) and the so-called nucleotides: riboflavin 5′-phosphate (flavin mononucleotide, FMN) and the intramolecular complex of FMN with adenosine 5′-monophosphate (fiavin adenine dinucleotide, FAD). Riboflavin is polymorphic—three different crystal forms of different solubility in water are known. This chapter discusses focuses on the fluorometric analyses of riboflavin and its coenzymes. The fluorometric determination of flavins are carried out in two ways—by the measurement of the intensity of the natural fluorescence of flavins and the fluorescence of lumifiavin derived from flavins. Both these methods can be equally applied by using similar analytic procedures according to certain physicochemical properties of flavins. These properties, important as a basis for the fluorometric determination are described.
Publication Year: 1971
Publication Date: 1971-01-01
Language: en
Type: book-chapter
Indexed In: ['crossref']
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Cited By Count: 153
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