Title: MICROFLUIDIC COUNTERFLOW CENTRIFUGAL ELUTRIATION FOR CELL SEPARATION USING DENSITY-GRADIENT MEDIA
Abstract:This paper reports a microfluidic system for cell separation employing counterflow centrifugal elutriation (CCE), combined with the density-gradient media and the branching inlet structures. In conven...This paper reports a microfluidic system for cell separation employing counterflow centrifugal elutriation (CCE), combined with the density-gradient media and the branching inlet structures. In conventional CCE, the balance of centrifugal force and fluidic force is gradually changed by increasing the flow rate and/or by decreasing the rotation speed, achieving the separation and recovery of cells based on size, shape, and/or density. In the presented system, both the centrifugal force and the driving force to transport fluids are generated by the device rotation at the same time. We successfully separated microparticles, and observed the retention behaviors of cells in the separation chamber. The pumpintegrated rotor systems required for conventional CCE are dispensed with by employing the density-gradient media, which remarkably simplified the instruments and procedures.Read More
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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Cited By Count: 5
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