Title: In-Vitro Plantlet Regeneration Through Somatic Embryogenesis in Solanum melongena (Thengaithittu Variety)
Abstract: 3 Abstract: The present investigation relates to the study of In vitro regeneration of Solanum melongena (Thengaithittu variety), which is an agronomically important crop grown primarily for its large oval fruit and its medicinal property. The embryo from mature seeds, cotyledon and shoot explants were inoculated on MS medium supplemented with various concentrations and combinations of 1-naphthaleneacetic acid, Kinetin, 2, 4-dichloro phenoxy acetic acid, Thidiazuron and Benzyl amino purine. Cotyledon cultures were highly responsive for callus production on medium supplemented with NAA (10.6 mg/l) alone. However embryo explants cultures showed good responses for callus production and embryogenesis on MS medium supplemented with NAA (8.0mg/l) and Kinetin (0.1mg/l). Callusing response for shoot culture was low. In the presence of NAA, the embryo and cotyledon cultures showed callusing response of 40% and 80% respectively. Callusing response for embryos was comparatively better on 2-4D containing media (45%). Cotyledon explants responded in a similar manner in the presence of 2-4D and NAA (75%). The embryo development occurred in the same medium that contained NAA (8.0mg/l) and Kinetin (0.1mg/l) in two months and a total of 85 embryos were developed. In callus cultures derived from cotyledon explants, a total of 65 embryos were developed in the presence of high concentration of NAA (10.6mg/l) alone. All these embryos derived from cotyledon, embryo and shoot explants were transferred to hormone free medium for germination. After 3-4 weeks the regenerated plantlets were transferred to green house.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
Access and Citation
Cited By Count: 12
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot