Title: Rapid microsatellite development in Gekko japonicus using sequenced restriction-site associated DNA markers
Abstract: Twelve polymorphic microsatellite loci were isolated in the Japanese gecko, Gekko japonicus.We genotyped one population from Wenzhou, Zhejiang Province, China (N = 36).The mean number of observed alleles per locus was 7.3 (range 4 to 13).Observed and expected heterozygosity values ranged from 0.200 to 0.944 and from 0.395 to 0.797, respectively.One locus (GJ20) showed significant departure from Hardy-Weinberg equilibrium; no linkage disequilibrium was found between any two loci.These informative microsatellite markers will be useful for population genetic analyses of G. japonicus and other species in the genus Gekko.