Title: The expression of cell cycle regulators in HPV - induced cervical carcinogenesis
Abstract: HPV (human papillomavirus) infection is associated with cervical carcinogenesis through an interaction between HPV oncogenic proteins and cell cycle regulators. However, the exact pathogenic mechanisms are not determined yet and therefore in the present study we evaluated the possible role of A1, B1, D1, E1 cyclins in a cohort of cervical cancer and cervical intraepithelial neoplasia (CIN) patients with or without HPV infection. In order to evaluate their role in cervical neoplasia, cervical tissue specimens from 140 women were investigated. HPV genotyping was performed using Linear Array HPV Genotyping Test (Roche) according to the manufacturer's instructions. In order to evaluate the gene expression levels of A1, B1, D1, E1 cyclins, isolated RNAs were reversed-transcribed and assayed in Taq-Man qPCR (Applied Biosystems). All abnormal biopsies presented different levels of cyclins expression regardless of the lesion severity. There were statistically significant differences between the levels of cyclins expression in CIN1 HPV positive and negative cases. In HPV positive samples, cyclins E1 and B1 were upregulated compared with the HPV negative biopsies (p=0.001/p=0.0032). In SCC (squamous cell carcinoma) cases, cyclin D1 expression was significantly downregulated in HPV positive cases as compared with HPV negative cases (p=0.0002). Similarly, there was a significant difference (p=0.005) in the expression of cyclin B1. We conclude that infection of cervical mucosa by high-risk HPV leads to deregulation of the cell cycle via altering the expression level of certain genes. Our results also provide evidence that cyclin expression could help in early diagnosis of cervical carcinoma.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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Cited By Count: 5
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