Title: Comparison of Two Antigens for Diagnosis of Trichinellosis in Pigs
Abstract: Abstract: Due to its role in human Trichinellosis, there are increasing global requirements f or reliable diagnosticmethod for Trichinella spiralis infection in pigs. Consequently, there is a need for selection of potentdiagnostic antigen that could be successfully utilized in the diagnosis. Currently, two antigens (somatic andexcretory-secretory antigens of T. spiralis larvae) were evaluated for the diagnosis of pig trichinellosis usingELISA. The ES antigen exhibited highest diagnos tic potential (88.3%) as compared to somatic antigen (68.3%).The diagnostic potential of ES antigen is attributed to five immunogenic bands identified by infected pig serain immunoblot. These bands are of molecular weight 127, 58, 35, 20.5 and 12 KDa. While in somatic antigen siximmunogenic bands of molecular weight 230, 89, 58, 27, 15 and 6 KDa were identified. In conclusion, monitorin gfor T. spiralis antibodies in swine farms should be based on screening pig sera by means of ELISA inwhich larval ES antigen was utilized.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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Cited By Count: 6
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