Title: Determination of Aflatoxins in Sesame, Rice, Millet and Acha from Nigeria using HPLC
Abstract:A hundred and twenty (120) Nigerian food commodities including; rice (15), madidi (rice product) (15), millet (15), fura (millet dough) (15), sesame (30) and fonio (30) were collected, subjected to af...A hundred and twenty (120) Nigerian food commodities including; rice (15), madidi (rice product) (15), millet (15), fura (millet dough) (15), sesame (30) and fonio (30) were collected, subjected to aflatoxin extraction and clean up procedures and analyzed quantitatively for aflatoxins using High performance liquid chromatography.Five of the rice samples contained aflatoxin B 1 within the range of 37.26-113.2µg/kg, for madidi samples, 7 contained AFB 1 within the range of 0.20-112.5µg/kg, 3 contained AFB 2 within the range of 0.95-18.40µg/kg while 6.88 µg/kg and 1.60 µg/kg of the green fluorescing aflatoxins 1 and 2 respectively was found in one sample each.Analysis of sesame samples showed that eight contained AFB 1 within the range of 14.71-140.9µg/kg, while one sample contained 2.61 µg/kg of aflatoxin G 1 .The millet, millet dough and fonio samples all tested negative for the presence of aflatoxins indicating some degree of resistance.All contaminated rice and sesame samples were at ranges above the EU and Nigerian legislated limits for AFB 1 and aflatoxins in food.This portrays associated health consequence to consumers and negative impact on trade of these commodities both locally and internationally.Read More