Title: Molecular genetic characterization of some biosurfactant- producing bacteria isolated from Egyptian red sea mangrove forests
Abstract: Biosurfactant are important biotechnological products, with a wide range of applications in many industries. In the present work, four soil bacterial strains were isolated, purified and tested for their natural ability to produce biosurfactants. Results of this experiment showed that the tested bacterial isolates were capable of producing biosurfactant. Traditional microbial characterization methods revealed that these bacterial strains belong to Bacillus subtilis, Bacillus coagulans, Bacillus amyloliquefaciens and Pseudomonas pseudoalcaligene. Moreover, molecular genetic characterization of the tested bacterial strains has been carried out via polymerase chain reactionrestriction fragment length polymorphism (PCR-16S rRNARFLP) and random amplified polymorphic DNA- PCR (RAPD-PCR). No genetic variations among the strains has been detected by the use of PCR-RFLP analysis. However, genetic dissimilarities have been detected via RAPDPCR. In addition, phylogenetic tree has been constructed based on RAPD-PCR analysis in which results indicated the discrimination of the three bacillus strains from the pseudomonas strains.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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Cited By Count: 4
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