Title: Variability in the Distribution of Daidzein and Genistein in Legume Sprouts and Their Anticancer Activity with MCF-7 Breast Cancer Cells
Abstract:The possible growth inhibitory properties of isoflavones extract of legume sprouts on anticancer activity was measured in human breast cancer cells (MCF-7) using MTT assay and compared with isoflavone...The possible growth inhibitory properties of isoflavones extract of legume sprouts on anticancer activity was measured in human breast cancer cells (MCF-7) using MTT assay and compared with isoflavones of soy beans sprouts in vitro. HPLC analysis of legume sprouts revealed the presence of daidzein and genistein as the predominant isoflavones in Cajanus cajan (Pigeon pea), Cicer arietinum (Chick pea), Glycine max (Soybean), Macrotyloma uniflorum (Horse gram), Lens culinaris (Lentil) and Pisum sativum (Garden pea). The most abundant isoflavone in the legume sprouts was found as genistein followed by daidzein. MTT assay demonstrated the dose dependent inhibition activities of daidzein and genistein on MCF-7 breast cancer cells. Lower concentrations of the extract did not exert a significant effect on cell growth inhibition and cell proliferation inhibition was more at higher concentrations of the extracts. G. max has recorded significant IC value against MCF-7 breast cancer cells followed by M. uniflorum and C. arietinum. The results depicted 50 the use of other legumes especially M. uniflorum and C. arietinum at their sprouts stage for preventing breast cancer apart from the commonly used G. max products.Read More
Publication Year: 2014
Publication Date: 2014-01-01
Language: en
Type: article
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Cited By Count: 10
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