Title: Evaluation of Genetic Variation between Thymus Accessions Using Molecular Markers
Abstract: Thirteen accessions of Thyme medicinal plant belonging to Iranian endemic species of Thymus daenansis, T. migricus, T. pubsence and T. fedtschenkoi were studied in aspect of molecular marker. The genomic DNA extracted using modified medicinal CTAB protocol. Evaluation of DNA quality was examined with electrophoresis. 20 primers were used for PCR analysis and only 8 primer showed clear bands. Out of 97 bands 61% were polymorphic. The data were analyzed with NTSYS program and the dendrogram was drawn based on UPGMA. Cluster analysis based on RAPD data allowed separating thyme in two groups at genetic distance of 80%. Group 1 was formed by T. daenensis, T. pubsence and T. migricus. Group 2 was formed by T. fedtschenkoi and T. daenensis subs daenensis (No. 7). Finally investigation the genetic variation with RAPD markers indicated that this marker is suitable to determine the polymorphic loci and to estimate the genetic distance.
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
Access and Citation
Cited By Count: 8
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