Title: Silver Nanoparticles Synthesized by Penicillium citreonigrum and Fusarium moniliforme Isolated from El-Sharkia, Egypt
Abstract:Background: Although nanoparticles can be made using various physicochemical methods that remains expensive and involves the use of hazardous chemicals. Biological synthesis of nanoparticles appears a...Background: Although nanoparticles can be made using various physicochemical methods that remains expensive and involves the use of hazardous chemicals. Biological synthesis of nanoparticles appears as a suitable process since it requires less energy, is environmentally safe, emerges as an eco-friendly, scalability, exciting approach, it has low manufacture costs of scalability, and better nanoparticle stabilization, compared to chemically synthesized nanoparticles. This study illustrates simple, green synthesis of AgNPs in vitro using cell lysate supernatant (CLS) of fungal species and to investigate its potential antimicrobial, antiviral activities, and cytotoxic effects against some tumor cell lines. The production ability of silver nanoparticles was investigated by the means of UV-V spectroscopy, electron microscopy and Xray microanalysis. Results: The production of silver nanoparticles by Penicillium citreonigrum and Fusarium moniliforme is reported in this study. The biosynthesized nanoparticles exhibited typical plasmon absorption maximum of silver nanoparticles (420nm).Spherical silver nanoparticles were found to have size between 10 and 50 nm by electron microscope analysis.X-ray pattern revealed the crystalline nature of the silver nanoparticles. Silver nanoparticles have broad spectrum antimicrobial activity against Gram positive and negative bacteria and fungi. In the meantime, AgNPs exhibited promising cytoprotective efficacy towards Herpes simplex type 2 virus and pronounced cytotoxic activity against some tumor cell lines. Conclusions: The studies showed that the microbial susceptibility to Ag-NPs is different for each microorganism.Penicillium citreonigrum and Fusarium moniliforme have capacity to biosynthesize silver nanoparticles, which are intracellularly accumulated. This property is present in whole cells and in free cell extracts indicating that this process is probably enzymatically mediated, due to the requirement of NADH as cofactor for this biological transformation.Read More
Publication Year: 2014
Publication Date: 2014-01-01
Language: en
Type: article
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Cited By Count: 5
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