Publication Information

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'strategy': [7, 105, 142, 240, 982, 1031, 1317, 3988], 'using': [8, 143, 297, 392, 443, 1047, 1305, 1338, 1350, 1931, 2352, 2445, 2773, 2802, 2838, 2914, 3174, 3203, 3957, 4151, 4369, 4417], 'affinity-purified': [9, 144, 1304, 3147, 3947], 'antibodies': [10, 145, 792, 893, 926, 963, 1026, 1038, 1043, 1086, 1103, 1300, 1777, 2972, 3148, 3948], 'raised': [11, 31, 146, 166], 'against': [12, 147], 'recombinant': [13, 148, 845, 3920, 4001], 'human': [14, 49, 79, 149, 184, 214, 682, 848, 875, 1154, 1220, 1257, 1352, 1818, 1859, 2023, 2245, 2287, 2441, 2503, 2535, 2551, 2845, 3178, 3245, 3409, 3815, 3877, 3958, 4047, 4239, 4306, 4349], 'protein': [15, 22, 34, 67, 86, 150, 157, 169, 202, 221, 287, 298, 370, 441, 491, 528, 587, 640, 660, 757, 973, 991, 1001, 1006, 1019, 1050, 1077, 1097, 1109, 1134, 1325, 1404, 1448, 1545, 1795, 2374, 2399, 2474, 2486, 2771, 2818, 2839, 2866, 2873, 2886, 2931, 2949, 2964, 3033, 3175, 3236, 3410, 3417, 3444, 3474, 3714, 3921, 3954, 4002, 4027, 4032, 4043, 4054, 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129, 194, 204, 210, 223, 251, 264, 289, 396, 418, 482, 520, 527, 825, 891, 951, 964, 975, 1067, 1203, 1280, 1291, 1299, 1303, 1327, 1367, 1493, 1562, 1583, 1598, 1628, 1650, 1667, 1708, 1759, 1889, 1902, 1904, 1923, 1944, 1975, 2025, 2086, 2104, 2177, 2208, 2222, 2417, 2435, 2468, 2556, 2660, 2673, 2678, 2700, 2719, 2794, 2821, 2858, 2871, 2896, 2978, 2980, 2985, 3085, 3199, 3384, 3426, 3491, 3531, 3554, 3584, 3623, 3631, 3641, 3649, 3671, 3675, 3711, 3731, 3736, 3923, 3971, 4004, 4056, 4075, 4096, 4109, 4140, 4143, 4345, 4356], 'success': [61, 196, 842, 2600, 2767, 3144], 'rates': [62, 197, 843], 'de': [64, 199], 'novo': [65, 200], 'cloning,': [66, 201], 'production,': [68, 203, 3926], 'antibody': [70, 205, 976, 3925, 4009], 'generation': [71, 206, 790, 924, 989, 1024, 1035, 1064, 1323, 2472, 3993, 4006], '85,': [73, 208], '76,': [74, 209], '56%,': [76, 211], 'respectively.': [77, 212], 'Using': [78, 213, 2035, 3915, 4248], 'tissue': [80, 215, 906, 1969, 2005, 2058, 2066, 2910, 3134, 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[276, 515], 'utilize': [279], 'information': [282, 1172], 'better': [284], 'understanding': [285], 'function.': [290], 'Most': [291], 'frequently,': [292], 'has': [294, 708, 1039, 3653], 'been': [295, 409, 652, 709, 942, 1040, 1116], 'done': [296, 2195], 'separation': [299, 1823], 'techniques': [300, 4372], 'coupled': [301], 'with': [302, 489, 947, 1123, 1130, 1265, 1288, 1343, 1569, 1640, 1722, 1734, 1746, 1766, 1812, 1896, 1906, 2008, 2122, 2187, 2401, 2437, 2488, 2494, 2629, 2646, 2788, 2867, 2874, 2888, 2973, 3021, 3111, 3367, 3488, 3533, 3544, 3565, 3592, 3894, 4202], 'mass': [303, 359, 397, 593, 3211], 'spectrometry': [304, 540], 'analysis': [305, 437, 638, 730, 1147, 2238, 2332, 3136, 3159, 3250, 3380, 3405, 3413, 3438, 4346], 'methods': [306, 407], '(1Patterson': [307], 'S.D.': [308], 'Aebersold': [309, 384], 'R.': [310, 385, 472, 605, 777, 911, 3865, 3867, 4334], 'Mass': [311, 539], 'spectrometric': [312], 'approaches': [313, 414, 971], 'identification': [316, 330, 543, 585], 'gel-separated': [318], 'proteins.': [319, 733, 796, 930, 4189], 'Electrophoresis.': [320], '1995;': [321, 374, 1806], '16:': [322], '1791-1814Google': [323], 'Scholar,': [324, 345, 377, 466, 504, 553, 599, 741, 773, 2387, 3055, 3072, 3311, 4401], '2Mann': [325], 'M.': [326, 328, 538, 603, 609, 621, 625, 627, 664, 712, 720, 743, 751, 857, 1224, 1246, 1390, 1398, 1434, 1442, 1531, 1539, 1789, 2254, 2276, 2676, 3295, 3319, 3782, 3804, 3855, 4330], 'Wilm': [327], 'Error-tolerant': [329], 'peptides': [332, 363], 'sequence': [334, 338, 1255, 2285, 2701, 3221, 3756, 3813, 4017], 'databases': [335], 'peptide': [337], 'tags.': [339], 'Anal.': [340, 372], 'Chem.': [341, 373, 3062, 3097], '1994;': [342], '66:': [343], '4390-4399Google': [344], '3Yates': [346], '3rd,': [347], 'J.R.': [348], 'Eng': [349, 380], 'J.K.': [350, 4380, 4409], 'McCormack': [351], 'A.L.': [352], 'Schieltz': [353], 'D.': [354, 506, 4336], 'Method': [355], 'correlate': [357], 'tandem': [358], 'spectra': [360], 'modified': [362], 'amino': [365, 1272, 2319, 3121, 3219, 3902, 4132, 4311], 'acid': [366, 1273, 1830, 3122, 3220, 4133], 'sequences': [367, 1213, 1501, 3123], 'database.': [371], '67:': [375], '1426-1436Google': [376], '4Han': [378], 'D.K.': [379, 1242, 2272, 3800], 'J.': [381, 583, 613, 768, 1415, 1459, 1556, 1803, 2410, 2459, 3095, 3291, 3307, 3313, 3325, 4102, 4324, 4342], 'Zhou': [382], 'H.': [383, 629, 672, 865, 1230, 2260, 3788, 3869], 'Quantitative': [386], 'differentiation-induced': [389], 'microsomal': [390], 'isotope-coded': [393], 'spectrometry.': [398, 594], 'Nat.': [399, 3341], 'Biotechnol.': [400, 769, 797, 931, 1416, 1460, 1557], '2001;': [401, 550, 2413, 3308, 4352], '19:': [402], '946-951Google': [403], 'Scholar).': [404, 646, 694, 887, 937, 1420, 1464, 1809, 2294, 2484, 2691, 3101, 3346, 3888, 4426], 'Recently,': [405], 'several': [406, 830, 2904, 4367], 'have': [408, 434, 651, 941, 955, 965, 1115, 3747, 3832, 3928, 4161], 'described': [410, 1432, 1528, 1786, 3776], '"genome-based"': [412], 'aimed': [415, 788, 922], 'enumerate': [417], 'functionally': [419], 'catalogue': [420], 'all': [421, 1953, 2240, 2343, 2754, 3008, 3529], 'components': [423], 'gene-by-gene': [429], 'approach.': [430, 2540], 'Initially,': [431], 'these': [432, 699, 2345, 2418, 3946, 3962], 'studies': [433, 1184, 2953, 4118], 'involved': [435], 'networks': [439], 'interactions,': [442], 'genetic': [444, 456], 'tools': [445, 3951], 'such': [446, 833, 1092, 1121, 1200, 2014, 2449, 2933, 3668, 3720], 'two-hybrid': [448, 476], 'systems': [449], '(5Fields': [450], 'S.': [451, 474, 499, 508, 581, 670, 722, 745, 755, 775, 783, 863, 909, 917, 1252, 1392, 1402, 1436, 1446, 1533, 1543, 2282, 2680, 3323, 3810, 3847, 3863], 'Song': [452], 'O.': [453], 'novel': [455], 'system': [457, 727], 'detect': [459], 'protein-protein': [460], 'interactions.': [461], 'Nature.': [462, 595, 642, 1260, 2290, 3818, 3884, 4351], '1989;': [463, 4398, 4423], '340:': [464], '245-246Google': [465], '6Chien': [467], 'C.T.': [468], 'Bartel': [469], 'P.L.': [470, 3028], 'Sternglanz': [471], 'Fields': [473], 'system:': [477], 'method': [479], 'identify': [481], 'clone': [483, 2524, 3978, 4263], 'interact': [488], 'interest.': [493], 'Proc.': [494, 2376], 'Natl.': [495], 'Acad.': [496], 'Sci.': [497, 690, 883], 'U.': [498], 'A.': [500, 557, 559, 567, 611, 1226, 1236, 2256, 2266, 2364, 2389, 3317, 3784, 3794, 3851, 3861, 4340], '1991;': [501], '88:': [502], '9578-9582Google': [503], '7Auerbach': [505], 'Thaminy': [507], 'Hottiger': [509], 'M.O.': [510], 'Stagljar': [511], 'I.': [512, 579], 'interactive': [517], 'proteomics:': [518], 'facts': [519], 'perspectives.': [521], 'Proteomics.': [522, 549], '2002;': [523, 596, 643, 691, 800, 884, 934, 3885], '2:': [524], '611-623Google': [525], 'Scholar)': [526, 803, 1264, 1561, 3822, 4108], 'complex': [529, 1190], 'pull-outs': [530], '(8Kuster': [531], 'B.': [532, 2391, 3059, 4078, 4318], 'Mortensen': [533], 'P.': [534, 569, 607, 3321, 3859], 'Andersen': [535], 'J.S.': [536], 'Mann': [537], 'allows': [541, 4018], 'direct': [542], 'large': [547, 3356, 3931], 'genomes.': [548, 2409], '1:': [551], '641-650Google': [552], '9Ho': [554], 'Y.': [555, 1240, 1250, 2270, 2280, 3798, 3808, 3843], 'Gruhler': [556], 'Heilbut': [558], 'Bader': [560], 'G.D.': [561], 'Moore': [562], 'L.': [563, 575, 716, 747, 1394, 1438, 1535, 3315, 3853], 'Adams': [564], 'S.L.': [565], 'Millar': [566], 'Taylor': [568, 1227, 2257, 3785], 'Bennett': [570], 'K.': [571, 573, 1238, 2268, 3796, 4326, 4328, 4338], 'Boutilier': [572], 'Yang': [574], 'Wolting': [576], 'C.': [577, 623, 718, 753, 781, 915, 1400, 1444, 1541, 1791, 4320], 'Donaldson': [578], 'Schandorff': [580], 'Shewnarane': [582], 'Systematic': [584], 'complexes': [588], 'Saccharomyces': [590], 'cerevisiae': [591], '415:': [597, 644], '180-183Google': [598], '10Gavin': [600], 'A.C.': [601], 'Bosche': [602], 'Krause': [604], 'Grandi': [606], 'Marzioch': [608], 'Bauer': [610], 'Schultz': [612], 'Rick': [614], 'J.M.': [615], 'Michon': [616], 'A.M.': [617], 'Cruciat': [618], 'C.M.': [619], 'Remor': [620], 'Hofert': [622], 'Schelder': [624], 'Brajenovic': [626], 'Ruffner': [628], 'Functional': [630], 'organization': [631], 'yeast': [634], 'complexes.': [641], '141-147Google': [645], 'Furthermore,': [647, 3010], 'structural': [648], 'genomics': [649], 'strategies': [650], 'developed': [653], 'allow': [655, 1380, 1427, 3233, 3964, 4090], 'streamlined': [656], 'unit': [657], 'operations': [658], 'structure': [661], 'determination': [662], '(11Hammarström': [663, 856], 'Hellgren': [665, 858], 'N.': [666, 859, 3845], 'van': [667, 860], 'Den': [668, 861], 'Berg': [669, 862], 'Berglund': [671, 864], 'Härd': [673, 866], 'T.': [674, 867, 1232, 1248, 2262, 2278, 3790, 3806], 'Rapid': [675, 868], 'screening': [676, 869, 2828, 3983], 'improved': [678, 871], 'solubility': [679, 872], 'small': [681, 874, 3673], 'produced': [684, 877, 3261], 'fusion': [686, 879, 1698, 1781], 'proteinsEscherichia': [687, 880], 'coli.': [688, 881], 'Protein': [689, 734, 882], '11:': [692, 885], '313-321Google': [693, 886], 'As': [695, 1140, 2231, 2812], 'complement': [697], 'efforts,': [700, 4273], 'more': [702, 2733, 3391, 3436], 'general': [703, 724, 1165], 'tool': [704, 764, 1411, 1455, 1552], 'genome-based': [706], 'proposed': [710], '(12Larsson': [711], 'Brundell': [713, 748, 778, 912, 1395, 1439, 1536], 'E.': [714, 749, 779, 913, 1244, 1396, 1440, 1537, 2274, 2704, 3802], 'Nordfors': [715], 'Höög': [717, 752, 780, 914, 1399, 1443, 1540, 1790], 'Uhlén': [719, 750, 1397, 1441, 1538], 'Ståhl': [721, 754, 782, 916, 1401, 1445, 1542], 'bacterial': [725], 'functional': [729, 766, 824, 1413, 1457, 1554], 'cDNA-encoded': [732, 795, 929], 'Expr.': [735], 'Purif.': [736], '1996;': [737, 3098, 4105], '7:': [738, 3309], '447-457PubMed': [739], 'Google': [740, 2386, 3071], '13Larsson': [742], 'Gräslund': [744, 1391, 1435, 1532], 'Yuan': [746, 1393, 1437, 1534], 'High-throughput': [756, 1403, 1447, 1544], 'cDNA': [760, 1407, 1451, 1548], 'products': [761, 1152, 1408, 1452, 1469, 1549, 2593, 2696, 2835, 2995, 3106, 3168, 3470, 3480, 3703], 'genomics.': [767, 1414, 1458, 1555], '2000;': [770, 1261, 1417, 1461, 1558, 2291, 3065, 3819], '80:': [771, 1418, 1462, 1559], '143-157Google': [772, 1419, 1463, 1560], '14Gräslund': [774], 'Falk': [776, 910], 'high-stringency': [785, 919], 'concept': [787, 921], 'specific': [793, 927, 1072, 1171, 1377, 2804, 3153, 3206, 3552, 4008, 4026], 'Appl.': [798, 932], 'Biochem.': [799, 933], '35:': [801, 935], '75-82Google': [802, 936], 'which': [805, 811, 1112, 3996], 'protein-specific': [806], 'are': [809, 829, 852, 1009, 1044, 1087, 2326, 2862, 2988, 3471, 3484, 3704, 3949, 3997, 4036, 4049, 4067, 4071, 4110, 4135, 4215, 4280], 'generated,': [810], 'stepwise': [817], 'manner': [818], 'wide': [821, 1940], 'range': [822, 1187], 'biochemical': [826], 'studies.': [827, 3497], 'There': [828], 'challenges': [831], 'strategy,': [835, 4250], 'here': [836], 'called': [837], 'proteomics.': [839], 'First,': [840], 'bacteria': [851], 'normally': [853], 'relatively': [854, 1065], 'low': [855], 'Second,': [888, 4064], 'both': [889, 946, 1289, 2220, 2879, 3382, 3999], 'polyclonal': [890, 1037, 1056, 1102, 2796, 3941, 4199], 'monoclonal': [892, 1060, 1085], 'often': [894], 'give': [895], 'cross-reactivity': [896, 2429, 2938], 'or': [900, 1096, 1129, 1210, 2936, 2963, 3980], 'high': [902, 1028, 3690, 4100], 'background': [903], 'binding': [904, 2149, 2650, 3046, 4206], 'sections': [907, 2069, 2154, 2179], '(14Gräslund': [908], 'Third,': [938, 4127], 'high-throughput': [939, 3235, 3335], 'schemes': [940], 'difficult': [943, 4072], 'employ': [945], 'regard': [948], 'automation': [950], 'cost-efficiency.': [952], 'These': [953], 'problems': [954], 'seriously': [956], 'hampered': [957], 'whole': [958, 1168, 4015, 4271], 'applications': [960], 'generate': [962, 3970, 4183], 'prompted': [966], 'us': [967, 2565], 'try': [969], 'new': [970, 2471, 2581, 3299], 'generation.': [977], 'describe': [980, 1145], '(PrESTs).': [995], '1The': [996], 'abbreviation': [997], 'is:': [999], 'PrEST,': [1000], 'tag.': [1004], '(PrESTs)': [1008], 'designed': [1010, 1161, 2516], 'contain': [1012, 2311], 'epitopes': [1014, 4148, 4207, 4214], 'present': [1015, 4216], 'native': [1018, 4164], 'suitable': [1020, 2338], 'triggering': [1022], 'selectivity.': [1029], 'followed.': [1041], 'purified': [1046, 1512, 1724, 2167, 2792, 2801], 'target': [1049, 1076], 'ligand.': [1052], 'use': [1054, 1100, 3972, 4198, 4292, 4388], 'antibodies,': [1057, 1061, 2176, 2827], 'instead': [1058], 'makes': [1062, 2947, 4179], 'cost-effective': [1066, 3924], 'increases': [1068], 'probability': [1070, 4211], 'recognition': [1073], 'during': [1078, 1800, 4062, 4217], 'variety': [1080, 3243], 'denaturating': [1082], 'conditions.': [1083], 'Although': [1084, 1157], 'attractive': [1088, 4181], 'routine': [1090], 'use,': [1091], 'diagnostics,': [1094], 'therapeutics,': [1095], 'suited': [1106, 4115], 'profiling,': [1110, 3237], 'denaturated': [1117, 4177, 4188], 'different': [1119, 1519, 1817, 2030, 3362, 3394, 3569, 3701, 4222], 'conditions,': [1120], 'formalin': [1124], 'paraffin-imbedded': [1127], 'SDS': [1131], 'extracts': [1135, 2840, 3176], 'analyzed': [1136, 3748], 'Western': [1138, 1810, 2849, 3135, 3158, 3172], 'blots.': [1139], 'pilot': [1142, 3827], 'project,': [1143], '21.': [1156, 1259, 2289, 3759, 3817], 'project': [1159], 'provide': [1163, 4146], 'proof-of-concept': [1166], 'analysis,': [1170, 1298, 2820], 'gained': [1173], 'from': [1174, 1208, 1483, 1815, 1949, 1955, 1979, 1990, 2001, 2006, 2029, 2049, 2533, 2550, 2594, 2745, 2841, 2970, 3119, 3177, 3217, 3267, 3753, 4237, 4283], '21-encoded': [1177], 'may': [1179], 'valuable': [1181, 2562], 'diseases': [1191], 'map': [1193, 3875], 'chromosome,': [1196, 2304], 'also': [1197, 2033, 2955, 3115, 4434], 'including': [1198, 3581], 'disorders': [1199], 'cancer': [1202], 'Down': [1204], 'syndrome': [1205], 'result': [1207], 'deletion': [1209], 'duplication': [1211], 'chromosome.': [1216], 'All': [1217, 2692], '(15Hattori': [1223, 2253, 3781], 'Fujiyama': [1225, 2255, 3783], 'T.D.': [1228, 2258, 3786], 'Watanabe': [1229, 2259, 3787], 'Yada': [1231, 2261, 3789], 'Park': [1233, 2263, 3791], 'H.S.': [1234, 2264, 3792], 'Toyoda': [1235, 2265, 3793], 'Ishii': [1237, 2267, 3795], 'Totoki': [1239, 2269, 3797], 'Choi': [1241, 2271, 3799], 'Soeda': [1243, 2273, 3801], 'Ohki': [1245, 2275, 3803], 'Takagi': [1247, 2277, 3805], 'Sakaki': [1249, 2279, 3807], 'Taudien': [1251, 2281, 3809], 'DNA': [1254, 2284, 3812, 4294], '405:': [1262, 2292, 3820], '311-319Google': [1263, 2293, 3821], 'open': [1266, 2313, 3896], 'reading': [1267, 2314, 3897], 'frames': [1268], 'larger': [1269, 3836, 3899], 'than': [1270, 2317, 2734, 3392, 3900], '100': [1271, 1620, 1767, 2318, 3901], 'residues': [1274, 4134], 'subjected': [1276], 'PrEST': [1278, 1725, 2339, 3205, 4431], 'design': [1279], 'production.': [1281], 'set': [1283, 3932], 'PrESTs': [1285, 1307, 2793, 2805, 4158, 4360], 'representing': [1286], 'known': [1290, 2868, 2974, 2993, 3016, 3482], 'unknown': [1292, 2875, 2981, 3108, 3408, 3545], 'function': [1293, 2869, 2876, 2987, 3483, 3546], 'selected': [1295, 1954, 2054, 2520, 3468, 4030], 'further': [1297, 2952], 'generated': [1302, 3929], 'ligands.': [1309], 'profiles': [1326, 3013, 3475, 3716, 3956], 'data': [1330, 3024], 'genome-wide': [1333], 'level.': [1334], 'RT-PCR': [1335, 1342, 2539, 2607, 4246], 'performed': [1337, 1701, 1784, 2236, 2837, 4175, 4254], 'Superscript': [1339], 'One': [1340], 'Step': [1341], 'Platinum': [1344], 'Taq': [1345], '(Life': [1346], 'Technologies,': [1347], 'Rockville,': [1348], 'MD)': [1349], 'Total': [1353], 'RNA': [1354, 2532, 2545, 4235], 'Panel': [1355], 'IV': [1356], '(CLONTECH,': [1357], 'Palo': [1358], 'Alto,': [1359], 'CA)': [1360, 1837], 'template.': [1362], 'Flanking': [1363], 'restriction': [1364, 4390], 'sites': [1365], 'NotI': [1366], 'AscI,': [1368], 'respectively,': [1369], 'introduced': [1371], 'into': [1372, 1384, 1491, 1495, 1524, 2623], 'through': [1375, 4261], 'primers': [1378, 2582], '"in': [1381], 'frame"': [1382], 'cloning': [1383, 1429, 4139, 4252], 'vector': [1387, 2626], 'pAff8c': [1388], '(13Larsson': [1389, 1433, 1530], 'downstream': [1422], 'primer': [1423, 2513], 'biotinylated': [1425, 1467], 'solid-phase': [1428], 'previously': [1431, 1787, 3022], 'resulting': [1466], 'PCR': [1468, 2512, 2592], 'immobilized': [1471, 1682, 1689], 'onto': [1472, 2075], 'Dynabeads': [1473], 'M280-streptavidin': [1474], '(Dynal': [1475], 'Biotech,': [1476], 'Oslo,': [1477], 'Norway).': [1478], 'released': [1482], 'solid': [1485], 'support': [1486], 'NotI-AscI': [1488], 'digestion,': [1489], 'ligated': [1490], 'pAff8c,': [1492], 'transformed': [1494], 'Escherichia': [1496, 4085], 'coli': [1497, 2705], 'BL21(DE3)': [1498, 1515], 'cells.': [1499], 'clones': [1504, 2706, 4260], 'verified': [1506], 'dye-terminator': [1508], 'cycle': [1509], 'sequencing': [1510, 4344], 'plasmid': [1513], 'DNA.': [1514], 'cells,': [1516], 'harboring': [1517], 'constructs,': [1521], 'inoculated': [1523], 'culture': [1525], 'medium': [1526], 'before': [1529, 2857], 'induced': [1563], 'at': [1564, 1576, 1589, 1633, 1958, 2131, 2183, 2196, 4099], 'A600': [1565], 'nm': [1566], '≈': [1567], '1.0': [1568, 3371], 'isopropyl-β-d-thiogalactopyranoside': [1570], '(Sigma': [1571], 'Aldrich,': [1572], 'St.': [1573], 'Louis,': [1574], 'MO)': [1575], 'final': [1578, 2057], 'concentration': [1579], '1': [1581], 'mm,': [1582], 'incubation': [1585], 'continued': [1587], 'overnight': [1588, 2182], '25': [1590], '°C.': [1591, 1635, 2185], 'cells': [1593, 3422, 3430, 3530, 3590, 3634, 3684], 'harvested': [1595], 'centrifugation,': [1597, 1661], 'pellet': [1600], 'resuspended': [1602], '5': [1604], 'ml': [1605], 'lysis': [1606], 'buffer': [1607, 1831, 1852], '(7': [1608], 'm': [1609, 2099], 'guanidinium-HCl,': [1610], '47': [1611], 'mm': [1612, 1615, 1618, 1621, 1626, 3372], 'Na2HPO4,': [1613], '2.65': [1614], 'NaH2PO4,': [1616], '10': [1617, 2129], 'Tris-HCl,': [1619], 'NaCl,': [1622], 'pH': [1623, 2102], '8.0,': [1624], '20': [1625, 2144], 'β-mercaptoethanol)': [1627], 'incubated': [1629, 1895, 2181], '2': [1631], 'h': [1632], '37': [1634], 'solutions': [1637], 'sheared': [1639], 'bore': [1642], '0.8-mm': [1643], 'needle': [1644], '(Becton': [1645, 1654], 'Dickinson,': [1646], 'Franklin': [1647], 'Lakes,': [1648], 'NJ)': [1649], '10-ml': [1652], 'syringe': [1653], 'Dickinson)': [1655], 'reduce': [1657, 2147], 'viscosity.': [1659], 'After': [1660], 'supernatants,': [1663], 'containing': [1664, 1993, 3360], 'denatured': [1666], 'solubilized': [1668], 'products,': [1670], 'filtered': [1672], '(0.45': [1673], 'μm,': [1674], 'Sartorius': [1675], 'AG,': [1676], 'Goettingen,': [1677], 'Germany)': [1678], 'prior': [1679], 'metal': [1683, 1690, 1704], 'ion': [1684, 1691], 'chromatography': [1686, 1693], 'procedure.': [1687], 'purification': [1694, 2640, 2861, 2883], 'His6-tagged': [1697], 'TALON': [1703], '(Co2+)': [1705], 'resins': [1707], 'gravity': [1709], 'columns': [1710], '(CLONTECH)': [1711], 'recommended': [1713], 'manufacturer.': [1716], 'New': [1717, 2689], 'Zealand': [1718], 'rabbits': [1719, 1742, 2785], 'immunized': [1721, 1744, 2787], 'AgriSera': [1728], 'AB': [1729], '(Vännäs,': [1730], 'Sweden)': [1731], 'accordance': [1733, 3020, 3487], 'national': [1736], 'guidelines': [1737], '(Swedish': [1738], 'permit': [1739], 'no.A125/00).': [1740], 'subcutaneously': [1745], '200': [1747], 'μg': [1748, 1768], 'antigen': [1750, 1770, 2092], "Freund's": [1752, 1772], 'complete': [1753, 2408], 'adjuvant': [1754], 'primary': [1757, 2152, 2175], 'immunization': [1758], 'boosted': [1760], 'three': [1761, 2044], 'times': [1762], '3-week': [1764], 'intervals': [1765], 'incomplete': [1773], 'adjuvant.': [1774], 'Enrichment': [1775], 'reactive': [1778], '(16Starborg': [1788], 'murine': [1793], 'replication': [1794], 'P1': [1796], 'differentially': [1798], 'expressed': [1799, 2707, 3388, 3419, 3508, 3526, 3664], 'spermatogenesis.': [1801], 'Eur.': [1802], 'Cell': [1804], 'Biol.': [1805, 2382, 2412, 3063, 3096, 4104], '68:': [1807], '206-210Google': [1808], 'blots': [1811], 'extracted': [1814, 4236], '14': [1816], 'prepared': [1821], 'Novex': [1825], 'Nupage': [1826, 1850], 'gels': [1827], '(4–12%,': [1828], '4-morpholineethanesulfonic': [1829], 'system,': [1832, 1922], 'Novex,': [1833], 'Invitrogen,': [1834], 'San': [1835, 1936], 'Diego,': [1836], 'followed': [1838], 'transfer': [1840, 1851], 'polyvinylidene': [1842], 'difluoride': [1843], 'membranes': [1844, 1893], '(Immobilon-P,': [1845], 'Millipore,': [1846], 'Bedford,': [1847], 'MA)': [1848], '(according': [1853], "manufacturer's": [1856], 'protocol).': [1857], 'skin': [1862], '(SK),': [1863], 'breast': [1864], '(BR),': [1865], 'omental': [1866], 'fat': [1867], '(OF),': [1868], 'tonsil': [1869], '(TO),': [1870], 'placenta': [1871], '(PL),': [1872], 'lung': [1873], '(LU),': [1874], 'kidney,': [1875], '(KI),': [1876], 'liver': [1877, 1987, 1995, 1998, 2557, 3036], '(LI),': [1878], 'gallbladder': [1879], '(GB),': [1880], 'colon': [1881, 3425, 3670], '(CO),': [1882], 'ileum': [1883], '(IL),': [1884], 'duodenum': [1885], '(DU),': [1886], 'ventricle': [1887], '(VE),': [1888], 'esophagus': [1890], '(ES).': [1891], 'affinity-enriched': [1898], '(1:75)': [1901], 'washed': [1903], 'probed': [1905], 'horseradish': [1907], 'peroxidase-conjugated': [1908, 2189], 'anti-rabbit': [1909, 2188], 'IgG': [1910], '(Sigma).': [1911], 'SuperSignal': [1912], 'West': [1913], 'Dura': [1914], '(Pierce,': [1915], 'Rockford,': [1916], 'IL)': [1917], 'detection': [1921], 'digital': [1924], 'images': [1925], 'chemiluminescense': [1928], 'monitored': [1930], 'ChemiImager': [1933], '(Alpha': [1934], 'Innotech,': [1935], 'Leandro,': [1937], 'CA).': [1938, 2117], 'spectrum': [1941], 'neoplastic': [1945], 'chosen': [1948, 1989, 2018, 3763, 4136], '52': [1950, 3361], 'donor': [1951, 3271], 'blocks,': [1952], 'archives': [1957], 'Department': [1960], 'Pathology,': [1962], 'University': [1963], 'Hospital,': [1964], 'Uppsala.': [1965], 'Selection': [1966], 'morphological': [1973], 'criteria,': [1974], 'samples': [1976, 2028], 'taken': [1978], 'morphologically': [1980, 2002], 'areas': [1982, 2051], 'surrounding': [1983], 'tissue,': [1985], 'e.g.': [1986], 'block': [1992, 2060, 3283, 3349], 'outside': [1996], 'metastasis,': [1999], 'heart': [2000], 'myocardial': [2004], 'patient': [2007], 'cardiomyopathy,': [2009], 'etc.': [2010], 'In': [2011, 2728, 2852, 2878, 2966, 3452, 3680, 3693, 4154], 'all,': [2012], '43': [2013], 'representative': [2020], 'organs': [2024], 'Nine': [2027], 'tumors': [2031], 'included.': [2034], 'Tissue': [2037, 3296, 3332], 'arrayer': [2038], '(Beecher': [2039], 'Instruments,': [2040], 'Silver': [2041], 'Spring,': [2042], 'MD),': [2043], '1.0-mm': [2045], 'punches': [2046], 'acquired': [2048], 'defined': [2050], 'each': [2053, 2789, 3213], 'tissue.': [2055], 'array': [2059, 3377, 3404, 3460], 'contained': [2061], '156': [2062], '(12': [2063], '×': [2064], '13)': [2065], 'cylinders.': [2067], 'Paraffin': [2068], '4-μm': [2071], 'thickness': [2072], 'placed': [2074], 'Superfrost/plus®': [2076], 'slides': [2077, 2094, 2119, 2217, 3359], '(Roche': [2078], 'Applied': [2079], 'Science,': [2080], 'Basel,': [2081], 'Switzerland),': [2082], 'deparaffinized': [2083], 'xylene,': [2085], 'rehydrated': [2087], 'graded': [2089], 'alcohols.': [2090], 'For': [2091, 2990, 3102, 3498], 'retrieval,': [2093], 'immersed': [2096], '0.01': [2098], 'citrate': [2100], 'buffer,': [2101], '6.0,': [2103], 'boiled': [2105], '7': [2107], 'min': [2108, 2130], 'Decloacing': [2111], 'chamber®': [2112], '(Biocare': [2113], 'Medical,': [2114], 'Walnut': [2115], 'Creek,': [2116], 'Alternatively,': [2118], 'treated': [2121], '0.05%': [2123], 'protease': [2124], 'type': [2125], 'VIII': [2126], '(Sigma)': [2127, 2203, 2211], 'room': [2132, 2197], 'temperature.': [2133], 'Endogenous': [2134], 'peroxidase': [2135], 'blocked': [2137], '0.3%': [2139], 'H2O2': [2140], 'PBS': [2142], 'min.': [2145, 2201], 'To': [2146, 2427, 2523, 3232], 'nonspecific': [2148], 'antisera,': [2153], 'pre-incubated': [2156], '0.5%': [2158], 'BSA-c®': [2159], '(Aurion,': [2160], 'Wageningen,': [2161], 'Netherlands)': [2163], 'PBS.': [2165], 'reagents,': [2169, 2433], 'diluted': [2170], '1:400,': [2171], '4': [2184, 3397], 'Incubation': [2186], 'Envision®': [2190], '(DAKO,': [2191], 'Copenhagen,': [2192], 'Denmark)': [2193], 'temperature': [2198], '30': [2200], 'Diaminobenzidine': [2202], 'chromogen,': [2207], 'Harris': [2209, 4337], 'hematoxylin': [2210], 'counterstaining.': [2215], 'Parallel': [2216], 'immunostained': [2219], 'manually': [2221], 'autostainer': [2225], '(Ventana': [2226], 'Medical': [2227], 'Systems,': [2228], 'Tucson,': [2229], 'Arizona).': [2230], 'starting': [2233], 'point,': [2234], 'bioinformatics': [2237], 'found': [2248, 2309, 3575, 3601, 3628], 'Hattori': [2250, 3778], 'et': [2251, 3779], 'al.': [2252, 3780], 'Out': [2295], '225': [2298, 3774], 'mapped': [2301], '168': [2305, 2511, 2572, 2613], 'them': [2307], 'frame': [2315, 2628, 2645, 3898], 'longer': [2316], 'acids': [2320, 4312], '(300': [2321], 'base': [2322], 'pairs).': [2323], 'summarized': [2327], 'Fig.': [2329, 2763, 2853, 2967, 3140, 3190, 3396, 3453, 3694], '1.': [2330, 2764, 3141], 'Sequence': [2331], 'algorithms': [2333, 2357, 4035], 'select': [2337], 'coding': [2340], 'genes.': [2346, 2597, 4299], 'Transmembrane': [2347], 'spanning': [2348], 'transmembrane': [2353, 2371, 2398, 4065], 'hidden': [2354, 2366, 2403], 'Markow': [2355], 'model': [2356, 2368], '(17Sonnhammer': [2358], 'E.L.': [2359, 2396], 'von': [2360, 2392], 'Heijne': [2361, 2393], 'G.': [2362, 2394, 3329], 'Krogh': [2363], 'Markov': [2367, 2404], 'predicting': [2370], 'helices': [2372], 'sequences.': [2375], 'Int.': [2377], 'Conf.': [2378], 'Intell.': [2379], 'Syst.': [2380], 'Mol.': [2381, 2411, 4103], '1998;': [2383, 3052, 3343], '6:': [2384], '175-182PubMed': [2385], '18Krogh': [2388], 'Larsson': [2390], 'Sonnhammer': [2395], 'Predicting': [2397], 'topology': [2400], 'model:': [2405], 'Application': [2406], '305:': [2414], '567-580Google': [2415], 'Scholar),': [2416, 4355], 'avoided': [2421], 'selection': [2424], 'PrESTs.': [2426], 'minimize': [2428], 'domains': [2436, 4044], 'homology': [2438, 2446, 2497], 'omitted': [2444], 'search': [2447, 2476], 'programs,': [2448], 'BLAST': [2451, 2467], '(19Altschul': [2452], 'S.F.': [2453], 'Madden': [2454], 'T.L.': [2455], 'Schaffer': [2456], 'A.A.': [2457], 'Zhang': [2458, 2460], 'Z.': [2461], 'Miller': [2462], 'W.': [2463, 4332], 'Lipman': [2464], 'D.J.': [2465], 'Gapped': [2466], 'PSI-BLAST:': [2469], 'database': [2475], 'programs.': [2477], 'Nucleic': [2478], 'Acids': [2479], 'Res.': [2480], '1997;': [2481], '25:': [2482], '3389-3402Google': [2483], 'fragments,': [2487], 'size': [2490, 2742, 2946, 3004, 4302], '100–150': [2492, 4131], 'residues,': [2493], 'lowest': [2496], 'thus': [2506, 2609, 4057], 'selected.': [2507], 'total': [2509], 'pairs': [2514], 'flank': [2518], '(PrEST)': [2521], 'regions.': [2522], 'PrESTs,': [2526, 3995], 'pool': [2530, 2546, 4233], 'various': [2534, 2833, 4262], 'Use': [2541], 'single': [2544, 2775, 3280, 4232], 'consisting': [2547, 2634, 3240, 3273], 'material': [2549], 'brain,': [2552], 'spinal': [2553], 'cord,': [2554], 'placenta,': [2555], 'proved': [2558], 'allowing': [2564, 2639], 'amplify': [2567], '120': [2568], 'out': [2569, 2611, 2780], '(71%)': [2574], 'first': [2577, 2814], 'attempt.': [2578], 'By': [2579], 'designing': [2580], 'those': [2584], 'failed,': [2587], 'second': [2589], 'round': [2590], 'yielded': [2591], '22': [2595], 'additional': [2596], 'overall': [2599, 2766, 3143], 'rate': [2601, 2768, 3145], 'two': [2604, 3707], 'rounds': [2605], '142': [2610, 2617, 2782], '(85%).': [2615], 'amplified': [2618], 'cloned': [2622, 2699], 'dual': [2631], 'tag': [2633], 'hexahistidyl': [2637], 'tag,': [2638], 'nickel': [2642], 'columns,': [2643], 'immunopotentiating': [2648], 'albumin': [2649], 'domain': [2651], '(20Ståhl,': [2652], 'S.,': [2653, 2657], 'Nilsson': [2654], 'J.,': [2655], 'Hober,': [2656], 'Uhlén,': [2658], 'M.,': [2659], 'Nygren': [2661], 'PÅ.': [2662], '1999.': [2663], 'Affinity': [2664], 'fusions,': [2665], 'expression,': [2667, 3922], 'Bioprocess': [2669], 'Technology:': [2670], 'Fermentation,': [2671], 'Biocatalysis': [2672], 'Bioseparation': [2674], '(Flickinger,': [2675], 'C.,': [2677], 'Drew,': [2679], 'W.,': [2681], 'ed.)': [2682], 'pp.': [2683], '8–22,': [2684], 'John': [2685], 'Wiley': [2686], '&': [2687], 'Sons,': [2688], 'YorkGoogle': [2690], 'amplification': [2695, 3302], 'successfully': [2698], 'verified.': [2702], 'liquid': [2709], 'cultures': [2710], 'lysed': [2712], 'presence': [2715], 'guanidinium': [2717], 'chloride,': [2718], 'cleared': [2721], 'lysates': [2722], 'passed': [2724], 'over': [2725], 'nickel-containing': [2726], 'matrices.': [2727], 'majority': [2730, 3617], 'cases,': [2732, 2880, 4156], '0.5': [2735], 'mg': [2736], 'right': [2741], 'obtained': [2744, 2913, 3006, 4150], '50-ml': [2746], 'cultures.': [2747], 'summary': [2749, 3128], 'shown': [2761, 2863, 3138, 3705], 'production': [2772, 4358], 'event': [2777], '108': [2779], '(76%).': [2783], 'Two': [2784], 'antisera': [2797], 'individually': [2799], 'ligands': [2807, 3935], 'obtain': [2809], 'PrEST-specific': [2810], 'antibodies.': [2811, 3942], 'step': [2815], 'validate': [2823], 'functionality': [2824], 'panel': [2843], 'blotting': [2850], 'format.': [2851], '2,': [2854], 'after': [2859], '(SOD1)': [2870], '(KIAA0539).': [2877], 'yields': [2884], 'pattern': [2887, 3642, 3740], 'bands': [2889, 2906, 3110], 'expected': [2892, 3003, 3112], 'sizes': [2893, 3113], '(16': [2894], 'kDa': [2895], '252': [2897], 'kDa,': [2898], 'respectively).': [2899], 'It': [2900, 4190], 'noteworthy': [2902], 'smaller': [2905, 3906], 'displaying': [2907], 'same': [2909, 3187], 'KIAA0539': [2916, 3662, 3691], 'reagent.': [2918, 2942], 'Plausible': [2919], 'explanations': [2920], 'could': [2923, 3908], 'existence': [2926], 'splice': [2928, 2961], 'variants,': [2929], 'proteolysis,': [2930], 'modifications,': [2932], 'glycosylation,': [2935], 'simply': [2937], 'difference': [2944], 'interesting': [2950], 'analyze': [2956, 3892], 'possibility': [2958], 'mRNA': [2960], 'variants': [2962], 'modifications.': [2965], '3,': [2968, 2997, 3125], 'examples': [2969, 3456], 'eight': [2971, 3467], '(CCT8,': [2975], 'SOD1,': [2976, 3193], 'CBR1,': [2977, 3194], 'ATP5O)': [2979], '(KIAA0539,': [2982], 'SAMSN-1,': [2983, 3197], 'WDR9,': [2984, 3198], 'ZNF294)': [2986], 'shown.': [2989, 3472], 'four': [2992, 3104, 3478, 4238], '(Fig.': [2996, 3124, 3373], 'A–D),': [2998], 'band': [3000, 3154], 'cases.': [3009], 'published': [3023], '(21Golden': [3025], 'T.R.': [3026], 'Pedersen': [3027], 'oligomycin': [3030], 'sensitivity': [3031], 'conferring': [3032], 'rat': [3035], 'mitochondrial': [3037, 3522], 'ATP': [3038, 3518], 'synthase:': [3039], 'Arginine': [3040], '94': [3041], 'important': [3043, 4227], 'OSCP': [3048], 'F1.': [3050], 'Biochemistry.': [3051], '37:': [3053], '13871-13881Google': [3054], '22Forrest': [3056], 'G.L.': [3057], 'Gonzalez': [3058], 'Carbonyl': [3060], 'reductase.': [3061], 'Interact.': [3064], '129:': [3066], '21-40Crossref': [3067], 'PubMed': [3068], 'Scopus': [3069], '(209)': [3070], '23Brown': [3073], 'C.R.': [3074], 'Doxsey': [3075], 'S.J.': [3076], 'Hong-Brown': [3077], 'L.Q.': [3078], 'Martin': [3079], 'R.L.': [3080, 3287], 'Welch': [3081], 'W.J.': [3082], 'Molecular': [3083], 'chaperones': [3084], 'centrosome.': [3087], 'role': [3089], 'TCP-1': [3091], 'microtubule': [3093], 'nucleation.': [3094], '271:': [3099], '824-832Google': [3100], 'function,': [3109], 'detected,': [3116], 'deduced': [3118, 3216], 'E–H).': [3126], 'determined': [3150, 3170], '54/96': [3161], '(56%).Fig.': [3162], '3Tissue': [3163], 'blot': [3173], 'order': [3181], '2.': [3191], 'CCT8,': [3192], 'ATP5O,': [3195, 3513], 'KIAA0539,': [3196], 'ZNF294': [3200, 3594, 3730], 'detected': [3202], 'reagents.': [3208, 3596, 4010, 4126], 'molecular': [3210, 3336], 'product': [3215], 'given.View': [3223], 'Large': [3224], 'Image': [3225], 'Figure': [3226], 'ViewerDownload': [3227], 'Hi-res': [3228], 'image': [3229], 'Download': [3230], '(PPT)': [3231], 'arrays': [3239, 3259], 'constructed': [3248], 'cellular': [3252, 3383], 'transferring': [3263], 'cylinders': [3264], 'multitude': [3269], 'blocks': [3272], 'archival': [3275], 'paraffin-embedded': [3276], 'recipient': [3281, 3348], 'paraffin': [3282], '(24Rimm': [3284], 'D.L.': [3285], 'Camp': [3286], 'Charette': [3288], 'L.A.': [3289], 'Costa': [3290], 'Olsen': [3292], 'D.A.': [3293], 'Reiss': [3294], 'microarray:': [3297], 'technology': [3300], 'resources.': [3305], 'Cancer': [3306], '24-31Google': [3310], '25Kononen': [3312], 'Bubendorf': [3314], 'Kallioniemi': [3316, 3330], 'Barlund': [3318], 'Schraml': [3320], 'Leighton': [3322], 'Torhorst': [3324], 'Mihatsch': [3326], 'M.J.': [3327], 'Sauter': [3328], 'O.P.': [3331], 'microarrays': [3333], 'tumor': [3339], 'specimens.': [3340], 'Med.': [3342], '4:': [3344], '844-847Google': [3345], 'subsequently': [3351], 'number': [3357], 'triplicate': [3365], 'spots': [3366], 'diameter': [3369], '4).': [3374], 'permitted': [3378], 'simultaneous': [3379], '50': [3393], 'shows': [3398, 3560], 'example': [3400], 'KIAA0539.': [3411], 'showed': [3414, 3689], 'glandular': [3421], 'smooth': [3428, 3682], 'muscle': [3429, 3683], 'beneath': [3431], 'colonic': [3433], 'mucosa.': [3434, 3679], 'detailed': [3437], 'crypts': [3440], 'revealed': [3441], 'localized': [3446], 'Goblet': [3449], 'cell': [3450], 'vacuoles.': [3451], '5,': [3454], 'illustrative': [3455], 'having': [3481], 'intracellular': [3490], 'suggested': [3494], 'earlier': [3496], 'example,': [3499, 3559], 'detoxification': [3501], 'enzyme,': [3502], 'superoxide': [3503], 'dismutase': [3504], '(SOD1),': [3505], 'highly': [3507, 4041], 'liver,': [3511], 'whereas': [3512], 'subunit': [3515], 'synthetase': [3519], 'F1': [3523], 'complex,': [3524], 'virtually': [3528], 'visualized': [3532], 'idiosyncratic': [3535], 'microgranular': [3536], 'staining': [3537, 3549, 3564], 'pattern.': [3538], 'recognizing': [3542], 'gave': [3547], 'distinct': [3548, 3624], 'patterns': [3550, 3698], 'yielding': [3551], 'profiles.': [3556], 'ZNF294,': [3557], 'predominantly': [3562], 'cytoplasmic': [3563], 'variable': [3566], 'strong': [3572], 'immunostaining': [3573], 'testis,': [3577], 'where': [3578], 'seminiferous': [3579], 'tubules': [3580], 'germinative': [3582], 'epithelia': [3583], 'Sertoli': [3585], 'Leydig': [3589], 'reacted': [3591], 'crescent-shaped': [3598], 'immunoreactivity': [3599, 3626, 3644], 'spermatids,': [3603], 'although': [3604, 3829], 'mature': [3605], 'sperms': [3606], 'appeared': [3607], 'negative.': [3608], 'WDR9': [3610, 3652], 'reagent': [3612], 'negative': [3614], 'tissues,': [3619, 3667], 'but': [3620, 3904], 'focal': [3622], 'granular': [3625], 'hepatocytes': [3630], 'zona': [3632], 'reticulosa': [3633], 'adrenal': [3637], 'gland.': [3638], 'resemble': [3645], 'lipofuscin': [3648], 'some': [3654, 4093, 4213], 'relation': [3655], 'lysosomal': [3658], 'deposition': [3659], 'lipofuscin.': [3661], 'mucus-producing': [3666], 'intestine': [3674], 'uterine': [3677], 'cervical': [3678], 'addition,': [3681], 'prostatic': [3687], 'gland': [3688], 'expression.': [3692, 4033], '6,': [3695], '12': [3700], 'brain': [3708], '(cerebrum': [3710], 'cerebellum).': [3712], 'Distinct': [3713], 'observed,': [3719], 'dendritic/axonal': [3723], 'positivity': [3724], 'MCM3,': [3726], 'C21orf33': [3732], 'Bergman': [3734], 'astrocytes,': [3735], 'mosaic': [3738], 'like': [3739], 'GABPA': [3742], 'cerebrum.': [3744], 'predicted': [3752, 4282], 'Chromosome': [3760], 'establish': [3765], 'applicability': [3767], 'study.': [3771, 3914], 'original': [3773], 'included': [3824, 3910, 4059], 'study,': [3828], 'recent': [3830], 'predictions': [3831], 'indicated': [3833], 'slightly': [3835], 'content': [3838], '238': [3840], '(26Gitton': [3842], 'Dahmane': [3844], 'Baik': [3846], 'Ruiz': [3848], 'i': [3849], 'Altaba': [3850], 'Neidhardt': [3852], 'Scholze': [3854], 'Herrmann': [3856], 'B.G.': [3857], 'Kahlem': [3858], 'Benkahla': [3860], 'Schrinner': [3862], 'Yildirimman': [3864], 'Herwig': [3866], 'Lehrach': [3868], 'Yaspo': [3870], 'M.L.': [3871], 'orthologues': [3880], 'mouse.': [3883], '420:': [3886], '586-590Google': [3887], 'We': [3889, 3943], 'chose': [3890], 'acids,': [3903], 'obviously': [3905], 'extended': [3913], 'combination': [3917], 'bioinformatics,': [3919], 'form': [3938], 'monospecific': [3940], 'useful': [3950], 'explore': [3953], 'arrays.': [3960], 'Together,': [3961], 'methodologies': [3963], 'without': [3975, 4255, 4386], 'dependence': [3976, 4256], 'repositories': [3979], 'cumbersome': [3981, 4364], 'procedures.': [3984, 4225], 'amenable': [3998], 'availability': [4012, 4258], 'most': [4020, 4155], '(nonhomologous)': [4022], 'region': [4023], 'within': [4024], 'Comparative': [4034], 'ensure': [4039], 'homologous': [4042], 'not': [4050, 4058, 4111, 4149, 4160], 'being': [4051], 'immunogens': [4061], 'immunization.': [4063], 'omitted,': [4068], 'because': [4069, 4274], 'they': [4070], 'express': [4074], 'purify': [4076], '(27Miroux': [4077], 'Walker': [4079], 'J.E.': [4080], 'Over-production': [4081], 'coli:': [4086], 'Mutant': [4087], 'hosts': [4088], 'synthesis': [4091], 'membrane': [4094], 'globular': [4097], 'levels.': [4101], '260:': [4106], '289-298Google': [4107], 'likely': [4112], 'immunolocalization': [4117], 'due': [4119], 'less': [4121], 'accessibility': [4122], 'facilitate': [4138], 'possibly': [4145], 'conformational': [4147], 'shorter': [4152], 'peptides.': [4153], 'will': 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