Publication Information

{'id': 'https://openalex.org/W2168277561', 'doi': 'https://doi.org/10.1074/jbc.m504070200', 'title': 'Architecture of the Human Ndc80-Hec1 Complex, a Critical Constituent of the Outer Kinetochore', 'display_name': 'Architecture of the Human Ndc80-Hec1 Complex, a Critical Constituent of the Outer Kinetochore', 'publication_year': 2005, 'publication_date': '2005-06-17', 'ids': {'openalex': 'https://openalex.org/W2168277561', 'doi': 'https://doi.org/10.1074/jbc.m504070200', 'mag': '2168277561', 'pmid': 'https://pubmed.ncbi.nlm.nih.gov/15961401'}, 'language': 'en', 'primary_location': {'is_oa': True, 'landing_page_url': 'https://doi.org/10.1074/jbc.m504070200', 'pdf_url': 'http://www.jbc.org/article/S0021925820564715/pdf', 'source': {'id': 'https://openalex.org/S140251998', 'display_name': 'Journal of Biological Chemistry', 'issn_l': '0021-9258', 'issn': ['0021-9258', '1067-8816', '1083-351X'], 'is_oa': True, 'is_in_doaj': True, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320990', 'host_organization_name': 'Elsevier BV', 'host_organization_lineage': ['https://openalex.org/P4310320990'], 'host_organization_lineage_names': ['Elsevier BV'], 'type': 'journal'}, 'license': 'cc-by', 'license_id': 'https://openalex.org/licenses/cc-by', 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, 'type': 'article', 'type_crossref': 'journal-article', 'indexed_in': ['crossref', 'pubmed'], 'open_access': {'is_oa': True, 'oa_status': 'hybrid', 'oa_url': 'http://www.jbc.org/article/S0021925820564715/pdf', 'any_repository_has_fulltext': True}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5056320459', 'display_name': 'Claudio Ciferri', 'orcid': 'https://orcid.org/0000-0002-0804-2411'}, 'institutions': [{'id': 'https://openalex.org/I4210114103', 'display_name': 'FIRC Institute of Molecular Oncology', 'ror': 'https://ror.org/02hcsa680', 'country_code': 'IT', 'type': 'facility', 'lineage': ['https://openalex.org/I4210114103']}, {'id': 'https://openalex.org/I6141010', 'display_name': 'European Institute of Oncology', 'ror': 'https://ror.org/02vr0ne26', 'country_code': 'IT', 'type': 'healthcare', 'lineage': ['https://openalex.org/I4210153126', 'https://openalex.org/I6141010']}, {'id': 'https://openalex.org/I4210123579', 'display_name': 'Ripamonti', 'ror': 'https://ror.org/03vcgp521', 'country_code': 'IT', 'type': 'company', 'lineage': ['https://openalex.org/I4210123579']}], 'countries': ['IT'], 'is_corresponding': False, 'raw_author_name': 'Claudio Ciferri', 'raw_affiliation_strings': ['Department of Experimental Oncology, European Institute of Oncology, Via Ripamonti 435, 20141 Milan, Italy, the', 'Italian Foundation for Cancer Research (FIRC) Institute of Molecular Oncology Foundation, Via Adamello 16, 20139 Milan, Italy, the'], 'affiliations': [{'raw_affiliation_string': 'Italian Foundation for Cancer Research (FIRC) Institute of Molecular Oncology Foundation, Via Adamello 16, 20139 Milan, Italy, the', 'institution_ids': ['https://openalex.org/I4210114103']}, {'raw_affiliation_string': 'Department of Experimental Oncology, European Institute of Oncology, Via Ripamonti 435, 20141 Milan, Italy, the', 'institution_ids': ['https://openalex.org/I6141010', 'https://openalex.org/I4210123579']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5032742732', 'display_name': 'Jennifer De Luca', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I114027177', 'display_name': 'University of North Carolina at Chapel Hill', 'ror': 'https://ror.org/0130frc33', 'country_code': 'US', 'type': 'education', 'lineage': ['https://openalex.org/I114027177']}], 'countries': ['US'], 'is_corresponding': False, 'raw_author_name': 'Jennifer De Luca', 'raw_affiliation_strings': ['Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27599, the'], 'affiliations': [{'raw_affiliation_string': 'Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27599, the', 'institution_ids': ['https://openalex.org/I114027177']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5070838119', 'display_name': 'Silvia Monzani', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I6141010', 'display_name': 'European Institute of Oncology', 'ror': 'https://ror.org/02vr0ne26', 'country_code': 'IT', 'type': 'healthcare', 'lineage': ['https://openalex.org/I4210153126', 'https://openalex.org/I6141010']}, {'id': 'https://openalex.org/I4210123579', 'display_name': 'Ripamonti', 'ror': 'https://ror.org/03vcgp521', 'country_code': 'IT', 'type': 'company', 'lineage': ['https://openalex.org/I4210123579']}], 'countries': ['IT'], 'is_corresponding': False, 'raw_author_name': 'Silvia Monzani', 'raw_affiliation_strings': ['Department of Experimental Oncology, European Institute of Oncology, Via Ripamonti 435, 20141 Milan, Italy, the'], 'affiliations': [{'raw_affiliation_string': 'Department of Experimental Oncology, European Institute of Oncology, Via Ripamonti 435, 20141 Milan, Italy, the', 'institution_ids': ['https://openalex.org/I6141010', 'https://openalex.org/I4210123579']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5034028854', 'display_name': 'Karin Johanna Ferrari', 'orcid': 'https://orcid.org/0000-0002-9877-632X'}, 'institutions': [{'id': 'https://openalex.org/I4210123579', 'display_name': 'Ripamonti', 'ror': 'https://ror.org/03vcgp521', 'country_code': 'IT', 'type': 'company', 'lineage': ['https://openalex.org/I4210123579']}, {'id': 'https://openalex.org/I6141010', 'display_name': 'European Institute of Oncology', 'ror': 'https://ror.org/02vr0ne26', 'country_code': 'IT', 'type': 'healthcare', 'lineage': ['https://openalex.org/I4210153126', 'https://openalex.org/I6141010']}], 'countries': ['IT'], 'is_corresponding': False, 'raw_author_name': 'Karin J. Ferrari', 'raw_affiliation_strings': ['Department of Experimental Oncology, European Institute of Oncology, Via Ripamonti 435, 20141 Milan, Italy, the'], 'affiliations': [{'raw_affiliation_string': 'Department of Experimental Oncology, European Institute of Oncology, Via Ripamonti 435, 20141 Milan, Italy, the', 'institution_ids': ['https://openalex.org/I4210123579', 'https://openalex.org/I6141010']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5103618522', 'display_name': 'Dejan Ristić', 'orcid': None}, 'institutions': [], 'countries': [], 'is_corresponding': False, 'raw_author_name': 'Dejan Ristic', 'raw_affiliation_strings': ['Department of Radiation Oncology, Daniel Den Hoed Cancer Center and the'], 'affiliations': [{'raw_affiliation_string': 'Department of Radiation Oncology, Daniel Den Hoed Cancer Center and the', 'institution_ids': []}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5013000014', 'display_name': 'Claire Wyman', 'orcid': 'https://orcid.org/0000-0003-2549-6893'}, 'institutions': [{'id': 'https://openalex.org/I2801952686', 'display_name': 'Erasmus MC', 'ror': 'https://ror.org/018906e22', 'country_code': 'NL', 'type': 'healthcare', 'lineage': ['https://openalex.org/I2801952686']}], 'countries': ['NL'], 'is_corresponding': False, 'raw_author_name': 'Claire Wyman', 'raw_affiliation_strings': ['Department of Cell Biology and Genetics, Erasmus Medical Center, P. O. Box 1738, 3000 DR, Rotterdam, The Netherlands, the', 'Department of Radiation Oncology, Daniel Den Hoed Cancer Center and the'], 'affiliations': [{'raw_affiliation_string': 'Department of Radiation Oncology, Daniel Den Hoed Cancer Center and the', 'institution_ids': []}, {'raw_affiliation_string': 'Department of Cell Biology and Genetics, Erasmus Medical Center, P. O. Box 1738, 3000 DR, Rotterdam, The Netherlands, the', 'institution_ids': ['https://openalex.org/I2801952686']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5016614839', 'display_name': 'Holger Stark', 'orcid': 'https://orcid.org/0000-0002-6161-1118'}, 'institutions': [{'id': 'https://openalex.org/I4210131661', 'display_name': 'Max Planck Institute for Biophysical Chemistry', 'ror': 'https://ror.org/03e76ya46', 'country_code': 'DE', 'type': 'facility', 'lineage': ['https://openalex.org/I149899117', 'https://openalex.org/I4210131661']}], 'countries': ['DE'], 'is_corresponding': False, 'raw_author_name': 'Holger Stark', 'raw_affiliation_strings': ['Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Goettingen, Germany, and the'], 'affiliations': [{'raw_affiliation_string': 'Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Goettingen, Germany, and the', 'institution_ids': ['https://openalex.org/I4210131661']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5066605730', 'display_name': 'John V. Kilmartin', 'orcid': 'https://orcid.org/0000-0002-4520-2365'}, 'institutions': [{'id': 'https://openalex.org/I170203145', 'display_name': 'MRC Laboratory of Molecular Biology', 'ror': 'https://ror.org/00tw3jy02', 'country_code': 'GB', 'type': 'facility', 'lineage': ['https://openalex.org/I170203145', 'https://openalex.org/I4210087105', 'https://openalex.org/I90344618']}, {'id': 'https://openalex.org/I90344618', 'display_name': 'Medical Research Council', 'ror': 'https://ror.org/03x94j517', 'country_code': 'GB', 'type': 'government', 'lineage': ['https://openalex.org/I4210087105', 'https://openalex.org/I90344618']}], 'countries': ['GB'], 'is_corresponding': False, 'raw_author_name': 'John Kilmartin', 'raw_affiliation_strings': ['Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, United Kingdom'], 'affiliations': [{'raw_affiliation_string': 'Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, United Kingdom', 'institution_ids': ['https://openalex.org/I170203145', 'https://openalex.org/I90344618']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5112374864', 'display_name': 'Edward D. Salmon', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I114027177', 'display_name': 'University of North Carolina at Chapel Hill', 'ror': 'https://ror.org/0130frc33', 'country_code': 'US', 'type': 'education', 'lineage': ['https://openalex.org/I114027177']}], 'countries': ['US'], 'is_corresponding': False, 'raw_author_name': 'Edward D. Salmon', 'raw_affiliation_strings': ['Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27599, the'], 'affiliations': [{'raw_affiliation_string': 'Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27599, the', 'institution_ids': ['https://openalex.org/I114027177']}]}, {'author_position': 'last', 'author': {'id': 'https://openalex.org/A5073374028', 'display_name': 'Andrea Musacchio', 'orcid': 'https://orcid.org/0000-0003-2362-8784'}, 'institutions': [{'id': 'https://openalex.org/I4210114103', 'display_name': 'FIRC Institute of Molecular Oncology', 'ror': 'https://ror.org/02hcsa680', 'country_code': 'IT', 'type': 'facility', 'lineage': ['https://openalex.org/I4210114103']}, {'id': 'https://openalex.org/I6141010', 'display_name': 'European Institute of Oncology', 'ror': 'https://ror.org/02vr0ne26', 'country_code': 'IT', 'type': 'healthcare', 'lineage': ['https://openalex.org/I4210153126', 'https://openalex.org/I6141010']}, {'id': 'https://openalex.org/I4210123579', 'display_name': 'Ripamonti', 'ror': 'https://ror.org/03vcgp521', 'country_code': 'IT', 'type': 'company', 'lineage': ['https://openalex.org/I4210123579']}], 'countries': ['IT'], 'is_corresponding': True, 'raw_author_name': 'Andrea Musacchio', 'raw_affiliation_strings': ['Department of Experimental Oncology, European Institute of Oncology, Via Ripamonti 435, 20141 Milan, Italy, the', 'Italian Foundation for Cancer Research (FIRC) Institute of Molecular Oncology Foundation, Via Adamello 16, 20139 Milan, Italy, the'], 'affiliations': [{'raw_affiliation_string': 'Italian Foundation for Cancer Research (FIRC) Institute of Molecular Oncology Foundation, Via Adamello 16, 20139 Milan, Italy, the', 'institution_ids': ['https://openalex.org/I4210114103']}, {'raw_affiliation_string': 'Department of Experimental Oncology, European Institute of Oncology, Via Ripamonti 435, 20141 Milan, Italy, the', 'institution_ids': ['https://openalex.org/I6141010', 'https://openalex.org/I4210123579']}]}], 'institution_assertions': [], 'countries_distinct_count': 5, 'institutions_distinct_count': 8, 'corresponding_author_ids': ['https://openalex.org/A5073374028'], 'corresponding_institution_ids': ['https://openalex.org/I4210114103', 'https://openalex.org/I6141010', 'https://openalex.org/I4210123579'], 'apc_list': {'value': 2500, 'currency': 'USD', 'value_usd': 2500, 'provenance': 'doaj'}, 'apc_paid': {'value': 2500, 'currency': 'USD', 'value_usd': 2500, 'provenance': 'doaj'}, 'fwci': 5.576, 'has_fulltext': True, 'fulltext_origin': 'pdf', 'cited_by_count': 177, 'citation_normalized_percentile': {'value': 0.99998, 'is_in_top_1_percent': True, 'is_in_top_10_percent': True}, 'cited_by_percentile_year': {'min': 98, 'max': 99}, 'biblio': {'volume': '280', 'issue': '32', 'first_page': '29088', 'last_page': '29095'}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T10303', 'display_name': 'Photosynthetic Processes and Mechanisms', 'score': 0.9961, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, 'topics': [{'id': 'https://openalex.org/T10303', 'display_name': 'Photosynthetic Processes and Mechanisms', 'score': 0.9961, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T12094', 'display_name': 'Hemoglobin structure and function', 'score': 0.995, 'subfield': {'id': 'https://openalex.org/subfields/1307', 'display_name': 'Cell Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T10301', 'display_name': 'Mitochondrial Function and Pathology', 'score': 0.9933, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}], 'keywords': [], 'concepts': [{'id': 'https://openalex.org/C192838845', 'wikidata': 'https://www.wikidata.org/wiki/Q908912', 'display_name': 'Kinetochore', 'level': 4, 'score': 0.53569955}, {'id': 'https://openalex.org/C86803240', 'wikidata': 'https://www.wikidata.org/wiki/Q420', 'display_name': 'Biology', 'level': 0, 'score': 0.20243442}, {'id': 'https://openalex.org/C54355233', 'wikidata': 'https://www.wikidata.org/wiki/Q7162', 'display_name': 'Genetics', 'level': 1, 'score': 0.06585917}, {'id': 'https://openalex.org/C30481170', 'wikidata': 'https://www.wikidata.org/wiki/Q37748', 'display_name': 'Chromosome', 'level': 3, 'score': 0.052414298}, {'id': 'https://openalex.org/C104317684', 'wikidata': 'https://www.wikidata.org/wiki/Q7187', 'display_name': 'Gene', 'level': 2, 'score': 0.0}], 'mesh': [{'descriptor_ui': 'D018386', 'descriptor_name': 'Kinetochores', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': True}, {'descriptor_ui': 'D009687', 'descriptor_name': 'Nuclear Proteins', 'qualifier_ui': 'Q000737', 'qualifier_name': 'chemistry', 'is_major_topic': True}, {'descriptor_ui': 'D018797', 'descriptor_name': 'Cell Cycle Proteins', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D002845', 'descriptor_name': 'Chromatography', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D003001', 'descriptor_name': 'Cloning, Molecular', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D003598', 'descriptor_name': 'Cytoskeletal Proteins', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D018076', 'descriptor_name': 'DNA, Complementary', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D018076', 'descriptor_name': 'DNA, Complementary', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': False}, {'descriptor_ui': 'D019281', 'descriptor_name': 'Dimerization', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D004591', 'descriptor_name': 'Electrophoresis, Polyacrylamide Gel', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D005982', 'descriptor_name': 'Glutathione Transferase', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D005982', 'descriptor_name': 'Glutathione Transferase', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': False}, {'descriptor_ui': 'D006367', 'descriptor_name': 'HeLa Cells', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D006801', 'descriptor_name': 'Humans', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D018386', 'descriptor_name': 'Kinetochores', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D018625', 'descriptor_name': 'Microscopy, Atomic Force', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D008869', 'descriptor_name': 'Microtubule-Associated Proteins', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D008869', 'descriptor_name': 'Microtubule-Associated Proteins', 'qualifier_ui': 'Q000737', 'qualifier_name': 'chemistry', 'is_major_topic': False}, {'descriptor_ui': 'D008938', 'descriptor_name': 'Mitosis', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D009687', 'descriptor_name': 'Nuclear Proteins', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D009687', 'descriptor_name': 'Nuclear Proteins', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': False}, {'descriptor_ui': 'D011485', 'descriptor_name': 'Protein Binding', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D011487', 'descriptor_name': 'Protein Conformation', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D017434', 'descriptor_name': 'Protein Structure, Tertiary', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D011994', 'descriptor_name': 'Recombinant Proteins', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D011994', 'descriptor_name': 'Recombinant Proteins', 'qualifier_ui': 'Q000737', 'qualifier_name': 'chemistry', 'is_major_topic': False}], 'locations_count': 3, 'locations': [{'is_oa': True, 'landing_page_url': 'https://doi.org/10.1074/jbc.m504070200', 'pdf_url': 'http://www.jbc.org/article/S0021925820564715/pdf', 'source': {'id': 'https://openalex.org/S140251998', 'display_name': 'Journal of Biological Chemistry', 'issn_l': '0021-9258', 'issn': ['0021-9258', '1067-8816', '1083-351X'], 'is_oa': True, 'is_in_doaj': True, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320990', 'host_organization_name': 'Elsevier BV', 'host_organization_lineage': ['https://openalex.org/P4310320990'], 'host_organization_lineage_names': ['Elsevier BV'], 'type': 'journal'}, 'license': 'cc-by', 'license_id': 'https://openalex.org/licenses/cc-by', 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, {'is_oa': True, 'landing_page_url': 'https://doi.org/10.17615/qne7-db42', 'pdf_url': 'https://cdr.lib.unc.edu/downloads/6d570603k', 'source': {'id': 'https://openalex.org/S4306401075', 'display_name': 'Carolina Digital Repository (University of North Carolina at Chapel Hill)', 'issn_l': None, 'issn': None, 'is_oa': True, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I114027177', 'host_organization_name': 'University of North Carolina at Chapel Hill', 'host_organization_lineage': ['https://openalex.org/I114027177'], 'host_organization_lineage_names': ['University of North Carolina at Chapel Hill'], 'type': 'repository'}, 'license': 'cc-by', 'license_id': 'https://openalex.org/licenses/cc-by', 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, {'is_oa': False, 'landing_page_url': 'https://pubmed.ncbi.nlm.nih.gov/15961401', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4306525036', 'display_name': 'PubMed', 'issn_l': None, 'issn': None, 'is_oa': False, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}], 'best_oa_location': {'is_oa': True, 'landing_page_url': 'https://doi.org/10.1074/jbc.m504070200', 'pdf_url': 'http://www.jbc.org/article/S0021925820564715/pdf', 'source': {'id': 'https://openalex.org/S140251998', 'display_name': 'Journal of Biological Chemistry', 'issn_l': '0021-9258', 'issn': ['0021-9258', '1067-8816', '1083-351X'], 'is_oa': True, 'is_in_doaj': True, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320990', 'host_organization_name': 'Elsevier BV', 'host_organization_lineage': ['https://openalex.org/P4310320990'], 'host_organization_lineage_names': ['Elsevier BV'], 'type': 'journal'}, 'license': 'cc-by', 'license_id': 'https://openalex.org/licenses/cc-by', 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, 'sustainable_development_goals': [], 'grants': [], 'datasets': [], 'versions': [], 'referenced_works_count': 42, 'referenced_works': ['https://openalex.org/W1937702437', 'https://openalex.org/W1977907334', 'https://openalex.org/W1986092065', 'https://openalex.org/W1989054182', 'https://openalex.org/W2016373415', 'https://openalex.org/W2017914952', 'https://openalex.org/W2024645073', 'https://openalex.org/W2026443391', 'https://openalex.org/W2027329338', 'https://openalex.org/W2031284400', 'https://openalex.org/W2031349427', 'https://openalex.org/W2034700471', 'https://openalex.org/W2037607661', 'https://openalex.org/W2041052562', 'https://openalex.org/W2045369188', 'https://openalex.org/W2050911321', 'https://openalex.org/W2054673627', 'https://openalex.org/W2062117947', 'https://openalex.org/W2074286944', 'https://openalex.org/W2075393111', 'https://openalex.org/W2079195903', 'https://openalex.org/W2079741946', 'https://openalex.org/W2082708594', 'https://openalex.org/W2084567406', 'https://openalex.org/W2085165779', 'https://openalex.org/W2087625931', 'https://openalex.org/W2088006036', 'https://openalex.org/W2089844705', 'https://openalex.org/W2090336138', 'https://openalex.org/W2091239938', 'https://openalex.org/W2094533427', 'https://openalex.org/W2096509200', 'https://openalex.org/W2099009321', 'https://openalex.org/W2102673682', 'https://openalex.org/W2111644689', 'https://openalex.org/W2115960102', 'https://openalex.org/W2124362426', 'https://openalex.org/W2131863700', 'https://openalex.org/W2137141545', 'https://openalex.org/W2156482618', 'https://openalex.org/W2157980476', 'https://openalex.org/W2170499370'], 'related_works': ['https://openalex.org/W4399302834', 'https://openalex.org/W3200292899', 'https://openalex.org/W3025330221', 'https://openalex.org/W2944505386', 'https://openalex.org/W2799688312', 'https://openalex.org/W2357120530', 'https://openalex.org/W2313433448', 'https://openalex.org/W2168582956', 'https://openalex.org/W2142011899', 'https://openalex.org/W1988902633'], 'abstract_inverted_index': {'The': [0, 30, 84, 155, 178, 208, 262, 333, 412, 516, 1047, 1287, 2142, 2288, 2893, 2959, 3010, 3209, 3265, 3284, 3400, 3532, 3625, 3789, 3960, 4238], 'Ndc80': [1, 31, 45, 76, 90, 176, 179, 209, 223, 254, 268, 354, 1114, 1129, 1134, 1291, 1337, 1459, 1538, 1778, 1825, 2101, 2157, 2292, 2314, 2637, 2946, 3141, 3282, 3806, 3855, 3921, 3932], 'complex': [2, 32, 91, 101, 157, 180, 210, 269, 279, 335, 824, 1460, 1779, 1826, 2102, 2158, 2171, 2293, 2644, 2921, 2961, 3020, 3681, 3690, 3736, 3740, 3856], 'is': [3, 33, 49, 168, 181, 211, 227, 346, 571, 1130, 1202, 1292, 1539, 2149, 2159, 3329, 3336, 3343, 3352, 4185, 4234], 'a': [4, 15, 106, 114, 127, 170, 182, 193, 284, 292, 305, 348, 425, 430, 468, 849, 864, 869, 1119, 1332, 1542, 1560, 1656, 1662, 1678, 1792, 1800, 1803, 1828, 1835, 2104, 2118, 2295, 2335, 2623, 2646, 2686, 2702, 2750, 2811, 2944, 2982, 3086, 3092, 3129, 3186, 3200, 3396, 3451, 3489, 3619, 3769, 3785, 3813, 3824, 3853, 3881, 3890, 3905, 3935, 3950, 3954, 3971, 4055, 4150, 4251, 4354], 'constituent': [5, 183], 'of': [6, 10, 73, 87, 96, 109, 141, 147, 173, 184, 188, 251, 265, 274, 287, 319, 325, 351, 360, 364, 414, 467, 562, 568, 838, 1050, 1107, 1132, 1290, 1334, 1347, 1355, 1455, 1457, 1469, 1474, 1537, 1548, 1562, 1573, 1586, 1651, 1668, 1786, 1791, 1809, 1813, 1823, 1834, 1837, 2117, 2120, 2144, 2161, 2290, 2313, 2344, 2592, 2595, 2600, 2615, 2617, 2634, 2659, 2667, 2760, 2792, 2856, 2867, 2875, 2903, 2910, 2935, 2938, 3018, 3023, 3071, 3102, 3140, 3163, 3218, 3280, 3339, 3346, 3357, 3391, 3403, 3407, 3440, 3514, 3627, 3677, 3771, 3787, 3852, 3883, 3918, 3930, 4044, 4058, 4064, 4070, 4134, 4212, 4226, 4230, 4250, 4254, 4258, 4265, 4271, 4305, 4320, 4349, 4356], 'the': [7, 11, 20, 38, 71, 74, 88, 97, 110, 142, 148, 174, 185, 189, 198, 216, 249, 252, 266, 275, 288, 320, 326, 352, 358, 365, 464, 512, 566, 844, 858, 880, 886, 1003, 1101, 1105, 1113, 1133, 1205, 1335, 1344, 1352, 1458, 1478, 1529, 1567, 1571, 1584, 1587, 1594, 1666, 1672, 1777, 1784, 1807, 1821, 1824, 1844, 1848, 1853, 1858, 2100, 2109, 2115, 2125, 2129, 2145, 2152, 2156, 2169, 2291, 2311, 2317, 2342, 2345, 2593, 2598, 2613, 2630, 2635, 2642, 2660, 2798, 2919, 2932, 2939, 2954, 2965, 3034, 3063, 3068, 3137, 3216, 3261, 3276, 3281, 3290, 3301, 3337, 3344, 3347, 3353, 3358, 3370, 3377, 3381, 3388, 3404, 3441, 3512, 3515, 3516, 3734, 3738, 3870, 3919, 3924, 3927, 3945, 3976, 3987, 4008, 4021, 4062, 4131, 4207, 4223, 4227, 4248, 4255, 4266, 4303, 4315, 4347], 'outer': [8, 186, 872, 887, 1004, 1345, 1658, 1673, 1850, 2131], 'plate': [9, 187, 888, 1005, 1346], 'kinetochore': [12, 160, 190, 338, 636, 846, 1356, 1479, 1674, 1804, 1830, 1849, 1886, 2130, 2299, 2596, 2606], 'and': [13, 36, 44, 61, 102, 113, 121, 144, 150, 167, 191, 214, 222, 239, 280, 291, 299, 322, 328, 345, 471, 510, 565, 861, 1141, 1207, 1325, 1472, 1502, 1583, 1676, 1857, 1867, 1873, 1880, 2112, 2172, 2178, 2306, 2632, 2652, 2665, 2678, 2696, 2755, 2776, 2844, 2870, 2889, 2924, 2929, 2979, 3007, 3028, 3047, 3056, 3110, 3123, 3180, 3247, 3256, 3296, 3350, 3364, 3373, 3434, 3475, 3483, 3500, 3507, 3597, 3613, 3616, 3630, 3696, 3722, 3746, 3801, 3878, 3994, 4024, 4048, 4089, 4115, 4123, 4136, 4155, 4170, 4182, 4198, 4214, 4217, 4232, 4325], 'plays': [14, 192, 2103], 'critical': [16, 194, 1121], 'role': [17, 195, 1794, 1801, 2106, 2289], 'in': [18, 28, 164, 196, 206, 342, 437, 481, 623, 632, 857, 1061, 1123, 1204, 1302, 1466, 1481, 1544, 1555, 1559, 1604, 1665, 1671, 1795, 1802, 1843, 2107, 2113, 2124, 2137, 2601, 2625, 2701, 2717, 2749, 2757, 2964, 3213, 3294, 3602, 3618, 3666, 3699, 3899, 3902, 4051, 4195, 4236], 'establishing': [19, 197], 'stable': [21, 199, 507, 1599, 1787, 3998], 'kinetochore-microtubule': [22, 200, 1545, 1574, 1595, 1600, 1788, 2110, 2618], 'interactions': [23, 201, 1601, 3925], 'required': [24, 202, 1816], 'for': [25, 203, 573, 1058, 1299, 1783, 1817, 2301, 2673, 2732, 2746, 2807, 2912, 3053, 3062, 3193, 3523, 3797, 3805], 'chromosome': [26, 204, 1059, 1470, 1531], 'segregation': [27, 205], 'mitosis.': [29, 207], 'evolutionarily': [34, 212, 2163], 'conserved': [35, 213, 639, 1203, 2164], 'contains': [37, 215, 1138], 'four': [39, 53, 111, 217, 231, 289, 3928], 'subunits': [40, 54, 112, 218, 232, 290, 1456, 2680, 3929], 'Spc24,': [41, 219, 1140, 2675, 2693, 4047], 'Spc25,': [42, 220, 2676, 2694], 'Nuf2,': [43, 221, 1139, 2677, 2695, 4045], '(whose': [46, 224], 'human': [47, 75, 225, 253, 1208, 1288, 1336, 2636, 2674, 2687, 3931], 'homologue': [48, 226, 1289], 'called': [50, 228], 'Hec1).': [51, 229], 'All': [52, 230, 2671, 2711], 'are': [55, 92, 103, 233, 270, 281, 472, 638, 833, 1053, 1358, 2655], 'predicted': [56, 234, 4022, 4208, 4316], 'to': [57, 94, 235, 272, 362, 463, 474, 535, 642, 836, 868, 996, 1055, 1104, 1294, 1329, 1340, 1343, 1527, 1564, 1566, 1661, 1677, 1680, 1847, 2128, 2327, 2611, 2707, 2952, 2970, 3127, 3274, 3300, 3369, 3445, 3450, 3880, 3943, 3953, 3958, 3981, 4006, 4018, 4066, 4073, 4096, 4109, 4117, 4164, 4203, 4243, 4261, 4279, 4289, 4330], 'contain': [58, 236, 475], 'globular': [59, 237, 4023, 4210, 4318], 'domains': [60, 238, 4010], 'extensive': [62, 240], 'coiled': [63, 130, 152, 241, 308, 330, 4025, 4228], 'coil': [64, 242, 4026], 'regions.': [65, 243], 'To': [66, 244, 3510], 'gain': [67, 245], 'an': [68, 139, 246, 317, 853, 1883, 2162, 3716, 3966, 4103, 4128, 4299, 4343], 'insight': [69, 247], 'into': [70, 248, 3838], 'organization': [72, 250, 1201], 'complex,': [77, 255, 1135, 2947], 'we': [78, 256, 1326, 2621, 3031, 3933, 3990, 4060, 4139, 4148, 4201, 4246, 4313], 'reconstituted': [79, 257, 2605, 2643, 3857], 'it': [80, 258, 4184], 'using': [81, 259, 2604, 2685, 2810, 2848, 3091, 3812], 'recombinant': [82, 89, 156, 260, 267, 334, 3947], 'methods.': [83, 261], 'hydrodynamic': [85, 263], 'properties': [86, 264, 1049, 2654], 'identical': [93, 271], 'those': [95, 273, 2658], 'endogenous': [98, 175, 276, 353, 2661], 'HeLa': [99, 165, 277, 343, 1348, 1556, 3730], 'cell': [100, 278, 1349, 2332, 3675, 3832], 'consistent': [104, 282], 'with': [105, 283, 852, 2334, 2725, 2781, 2864, 2872, 2900, 2931, 2987, 3226, 3528, 3545, 3673, 3708, 3733, 3743, 3749, 3761, 3777, 3874, 3904, 3986, 4020, 4078, 4087, 4102, 4142, 4190], '1:1:1:1': [107, 285], 'stoichiometry': [108, 286], 'very': [115, 293], 'elongated': [116, 294], 'shape.': [117, 295], 'Two': [118, 296], 'tight': [119, 297, 4151], 'Hec1-Nuf2': [120, 149, 298, 327], 'Spc24-Spc25': [122, 151, 300, 329, 2894, 3679, 3995], 'subcomplexes,': [123, 301, 3517], 'each': [124, 302, 4012], 'stabilized': [125, 303, 4186], 'by': [126, 304, 529, 863, 1461, 1541, 1552, 2151, 2331, 2341, 2629, 2683, 2741, 2802, 2973, 3158, 3222, 3386, 3457, 3467, 3505, 3537, 4014, 4100, 4187, 4302, 4346], 'parallel': [128, 306], 'heterodimeric': [129, 307], 'coil,': [131, 309], 'maintain': [132, 310], 'this': [133, 311, 1124, 2626, 4004, 4296, 4339], 'organization.': [134, 312], 'These': [135, 313, 459, 2321, 3049], 'subcomplexes': [136, 314, 483, 505, 3485, 3999], 'tetramerize': [137, 315], 'via': [138, 316], 'interaction': [140, 318, 3464, 3513, 4129, 4152, 4189], 'C-': [143, 321], 'N-terminal': [145, 323, 4132, 4209, 4256], 'portions': [146, 324], 'coils,': [153, 331], 'respectively.': [154, 332], 'displays': [158, 336], 'normal': [159, 337, 1885], 'localization': [161, 339, 1585, 1887, 2116], 'upon': [162, 340, 4362], 'injection': [163, 341], 'cells': [166, 344, 1304, 1557, 1606, 1649, 3599, 3629, 3660, 3731, 3754, 3774, 3800, 3834], 'therefore': [169, 347], 'faithful': [171, 349], 'copy': [172, 350], 'complex.': [177, 355, 1338, 2638, 2662, 2941, 3283], 'Kinetochores': [356], 'mediate': [357], 'attachment': [359], 'chromosomes': [361, 525, 1563], 'microtubules': [363, 536, 993, 1357, 1669, 1683], 'mitotic': [366], 'spindle': [367, 513, 1568, 1859, 2318], '(1Cleveland': [368, 576, 1006], 'D.W.': [369, 577, 915, 1007], 'Mao': [370, 578, 1008], 'Y.': [371, 579, 670, 674, 702, 1009, 1307, 1430, 1754, 2019, 2061, 2189, 2476, 2518, 2581], 'Sullivan': [372, 580, 1010], 'K.F.': [373, 581, 896, 1011, 1033], 'Cell.': [374, 582, 653, 679, 726, 1012, 1315, 1443, 1767, 2032, 2084, 2213, 2489, 2541], '2003;': [375, 451, 495, 583, 654, 727, 769, 793, 815, 1013, 1152, 1219, 1248, 1393, 1616, 1717, 1900, 1929, 1982, 2069, 2214, 2256, 2280, 2357, 2386, 2439, 2526], '112:': [376, 584, 1014, 3315], '407-421Abstract': [377, 585, 1015], 'Full': [378, 380, 402, 404, 586, 588, 610, 612, 657, 659, 683, 685, 1016, 1018, 1222, 1224, 1277, 1279, 1396, 1398, 1619, 1621, 1720, 1722, 1903, 1905, 1958, 1960, 1985, 1987, 2088, 2090, 2360, 2362, 2415, 2417, 2442, 2444, 2545, 2547, 3584, 3586], 'Text': [379, 381, 403, 405, 587, 589, 611, 613, 658, 660, 684, 686, 1017, 1019, 1223, 1225, 1278, 1280, 1397, 1399, 1620, 1622, 1721, 1723, 1904, 1906, 1959, 1961, 1986, 1988, 2089, 2091, 2361, 2363, 2416, 2418, 2443, 2445, 2546, 2548, 3585, 3587], 'PDF': [382, 406, 590, 614, 661, 687, 1020, 1226, 1281, 1400, 1623, 1724, 1907, 1962, 1989, 2092, 2364, 2419, 2446, 2549, 3588], 'PubMed': [383, 407, 454, 498, 552, 591, 615, 662, 688, 711, 730, 755, 772, 796, 818, 905, 922, 935, 948, 967, 984, 1021, 1042, 1077, 1095, 1155, 1170, 1194, 1227, 1251, 1282, 1320, 1374, 1401, 1422, 1447, 1497, 1516, 1624, 1639, 1698, 1725, 1746, 1771, 1908, 1932, 1963, 1990, 2011, 2036, 2051, 2072, 2093, 2198, 2217, 2242, 2259, 2283, 2365, 2389, 2420, 2447, 2468, 2493, 2508, 2529, 2550, 2567, 2586, 3317, 3422, 3589, 3652, 4038], 'Scopus': [384, 408, 455, 499, 553, 592, 616, 663, 689, 712, 731, 756, 773, 797, 819, 906, 923, 936, 949, 968, 985, 1022, 1043, 1078, 1096, 1156, 1171, 1195, 1228, 1252, 1283, 1321, 1375, 1402, 1423, 1448, 1498, 1517, 1625, 1640, 1699, 1726, 1747, 1772, 1909, 1933, 1964, 1991, 2012, 2037, 2052, 2073, 2094, 2199, 2218, 2243, 2260, 2284, 2366, 2390, 2421, 2448, 2469, 2494, 2509, 2530, 2551, 2568, 2587, 3318, 3423, 3590, 3653, 4039], '(825)': [385, 593, 1023], 'Google': [386, 410, 457, 501, 555, 594, 618, 665, 691, 714, 733, 758, 775, 799, 821, 908, 925, 938, 951, 970, 987, 1024, 1045, 1080, 1098, 1158, 1173, 1197, 1230, 1254, 1285, 1323, 1377, 1404, 1425, 1450, 1500, 1519, 1627, 1642, 1701, 1728, 1749, 1774, 1911, 1935, 1966, 1993, 2014, 2039, 2054, 2075, 2096, 2201, 2220, 2245, 2262, 2286, 2368, 2392, 2423, 2450, 2471, 2496, 2511, 2532, 2553, 2570, 2589, 3320, 3425, 3592, 3655, 4041], 'Scholar,': [387, 595, 666, 692, 715, 734, 759, 776, 800, 909, 926, 939, 952, 971, 1025, 1081, 1159, 1174, 1231, 1255, 1378, 1405, 1426, 1628, 1702, 1729, 1750, 1912, 1936, 1967, 1994, 2015, 2040, 2055, 2076, 2202, 2221, 2246, 2263, 2369, 2393, 2424, 2451, 2472, 2497, 2512, 2533, 2554, 2571], '2Amor': [388, 596], 'D.J.': [389, 597, 1243, 1924, 2381], 'Kalitsis': [390, 598], 'P.': [391, 599, 762, 1145, 1311, 1432, 1756, 2021, 2078, 2249, 2478, 2535], 'Sumer': [392, 600], 'H.': [393, 601, 1064, 1215, 1484, 1612, 1896, 2063, 2353, 2520, 3416], 'Choo': [394, 602], 'K.H.': [395, 603], 'Trends': [396, 604], 'Cell': [397, 448, 492, 547, 605, 706, 791, 900, 917, 962, 1037, 1072, 1090, 1165, 1369, 1417, 1492, 1511, 1693, 1741, 2006, 2067, 2193, 2278, 2463, 2524, 2562, 3594, 3647], 'Biol.': [398, 450, 494, 548, 606, 707, 725, 792, 901, 918, 963, 1038, 1091, 1166, 1217, 1273, 1316, 1370, 1392, 1418, 1442, 1512, 1614, 1694, 1716, 1742, 1766, 1898, 1954, 1981, 2007, 2031, 2194, 2212, 2279, 2355, 2411, 2438, 2464, 2488, 2563, 3580, 3648], '2004;': [399, 607, 680, 708, 752, 1074, 1274, 1494, 1955, 2085, 2195, 2239, 2412, 2542, 2564], '14:': [400, 608, 728, 1275, 1956, 2215, 2413], '359-368Abstract': [401, 609], '(140)': [409, 617], 'Scholar).': [411, 458, 502, 556, 619, 822, 988, 1046, 1099, 1198, 1286, 1451, 1520, 1643, 1775, 2097, 2287, 2590, 3426, 3593, 3656], 'kinetochores': [413, 460, 532, 825, 990, 1350], 'Saccharomyces': [415], 'cerevisiae': [416], 'assemble': [417], 'on': [418, 2316, 3085, 3199, 3376, 3488, 3526, 3663, 3823, 3835, 3863, 3889], '∼150-base': [419], 'pair-long': [420], 'centromeric': [421, 569, 859], 'DNA': [422], 'wrapped': [423], 'around': [424], 'specialized': [426], 'nucleosome': [427], 'containing': [428, 2168, 3185, 3608], 'Cse4,': [429], 'histone': [431], 'H3': [432], 'variant': [433], 'known': [434, 878, 3117, 3262], 'as': [435, 848, 879, 1118, 1295, 1331, 1526, 1877, 2176, 2294, 2926, 3059, 3411, 3436, 3539, 3554, 3843, 3858, 3949, 3965, 3970], 'CENP-A': [436], 'higher': [438], 'eukaryotes': [439], '(3McAinsh': [440, 484], 'A.D.': [441, 485, 764, 1147, 2251], 'Tytell': [442, 486], 'J.D.': [443, 487], 'Sorger': [444, 488, 765, 1148, 2081, 2252, 2538, 2559], 'P.K.': [445, 489, 766, 1149, 2082, 2253, 2539, 2560], 'Annu.': [446, 490], 'Rev.': [447, 491, 545, 930], 'Dev.': [449, 493, 652, 751, 768, 814, 1151, 1247, 1928, 2083, 2238, 2255, 2385, 2540], '19:': [452, 496, 946], '519-539Crossref': [453, 497], '(180)': [456, 500], 'become': [461], 'attached': [462, 534], 'plus': [465, 999, 1353, 1589], 'end': [466], 'single': [469], 'microtubule': [470, 1588, 1796, 1818], 'estimated': [473, 3014], 'at': [476, 1477, 1593, 1841, 2122, 2735, 2743, 2804, 2858, 2915, 3015, 3076, 3196, 3206, 3468, 3758, 3768, 3784, 4352], 'least': [477, 3469, 4353], '60': [478], 'different': [479, 624, 4068], 'proteins': [480, 637, 1815, 1838, 1855, 1862, 1872, 1875, 2121, 2174, 3393, 3431, 3444, 3989], 'several': [482, 1462], 'Together,': [503], 'these': [504, 1605, 1889, 2138], 'produce': [506], 'DNA-microtubule': [508], 'bridges': [509], 'control': [511], 'assembly': [514, 1805, 1860, 2166, 2319, 2648], 'checkpoint.': [515, 2320], 'latter': [517], 'delays': [518], 'anaphase': [519], 'sister': [520, 531], 'chromatid': [521], 'separation': [522], 'until': [523], 'all': [524], 'have': [526, 626], 'achieved': [527], 'bio-orientation': [528], 'their': [530, 574, 3116, 3257, 3395, 3446, 3501], 'becoming': [533], 'from': [537, 640, 829, 885, 1535, 2324, 2657, 2786, 2898, 2957, 3135, 3289, 3297, 3912, 4360], 'opposite': [538], 'poles': [539], '(4Musacchio': [540], 'A.': [541, 749, 802, 808, 810, 1087, 1182, 1508, 2236, 2573, 3558, 3578, 4028], 'Hardwick': [542], 'K.G.': [543], 'Nat.': [544, 705, 2192], 'Mol.': [546, 724, 1314, 1441, 1765, 2030, 2211, 2487], '2002;': [549, 1419, 1636, 1743, 2008, 2048, 2465, 2505], '3:': [550], '731-741Crossref': [551], '(474)': [554], 'Human': [557, 3920], 'centromeres': [558], 'extend': [559], 'over': [560], 'millions': [561], 'base': [563], 'pairs,': [564], 'compaction': [567], 'chromatin': [570, 860], 'essential': [572, 1782], 'viability': [575], 'Recent': [620, 1644], 'proteomic': [621], 'efforts': [622], 'organisms': [625], 'clarified': [627], 'that': [628, 832, 837, 1576, 1598, 1648, 2155, 2337, 2641, 3067, 3992, 4176, 4183], 'despite': [629], 'significant': [630], 'differences': [631], 'centromere': [633], 'complexity,': [634], 'most': [635], 'yeast': [641, 1128], 'humans': [643], '(5Pinsky': [644], 'B.A.': [645], 'Tatsutani': [646], 'S.Y.': [647], 'Collins': [648], 'K.A.': [649], 'Biggins': [650], 'S.': [651, 721, 738, 740, 778, 782, 804, 839, 1085, 1506, 1630, 2042, 2208, 2225, 2227, 2265, 2269, 2499], '5:': [655], '735-745Abstract': [656], '(88)': [664], '6Hayashi': [667], 'T.': [668, 698, 806, 2057, 2059, 2065, 2185, 2514, 2516, 2522, 2575, 2579], 'Fujita': [669], 'Iwasaki': [671, 695, 2182], 'O.': [672, 696, 2183], 'Adachi': [673], 'Takahashi': [675], 'K.': [676, 747, 812, 2234], 'Yanagida': [677, 703, 2190], 'M.': [678, 704, 1361, 1685, 2191, 3570, 3574, 4031], '118:': [681], '715-729Abstract': [682], '(329)': [690], '7Obuse': [693], 'C.': [694, 1176, 1186, 2181, 2577], 'Kiyomitsu': [697, 2184], 'Goshima': [699, 2186], 'G.': [700, 789, 1388, 1712, 1977, 2187, 2276, 2434, 3641], 'Toyoda': [701, 2188], '6:': [709, 2196], '1135-1141Crossref': [710, 2197], '(214)': [713, 2200], '8Nekrasov': [716, 2203], 'V.S.': [717, 2204], 'Smith': [718, 2205], 'M.A.': [719, 2206], 'Peak-Chew': [720, 2207], 'Kilmartin': [722, 1162, 1412, 1736, 2001, 2209, 2458], 'J.V.': [723, 1163, 1413, 1737, 2002, 2210, 2459], '4931-4946Crossref': [729, 2216], '(77)': [732, 2219], '9Cheeseman': [735, 2222], 'I.M.': [736, 780, 2223, 2267], 'Niessen': [737, 2224], 'Anderson': [739, 781, 912, 2226, 2268], 'Hyndman': [741, 2228], 'F.': [742, 2229], 'Yates': [743, 783, 2230, 2270], 'III,': [744, 784, 2231, 2271], 'J.R.': [745, 785, 1239, 1920, 2232, 2272, 2377], 'Oegema': [746, 811, 2233], 'Desai': [748, 1086, 1507, 2235], 'Genes': [750, 767, 813, 1150, 1246, 1927, 2237, 2254, 2384], '18:': [753, 2240], '2255-2268Crossref': [754, 2241], '(337)': [757, 2244], '10De': [760, 2247], 'Wulf': [761, 1144, 2248], 'McAinsh': [763, 1146, 2250], '17:': [770, 816, 1093, 1153, 1249, 1318, 1514, 1930, 2257, 2387], '2902-2921Crossref': [771, 1154, 2258], '(236)': [774, 1157, 2261], '11Westermann': [777, 2264], 'Cheeseman': [779, 2266], 'Drubin': [786, 2273], 'D.G.': [787, 1365, 1689, 2274], 'Barnes': [788, 2275], 'J.': [790, 899, 916, 942, 958, 961, 1036, 1066, 1071, 1164, 1178, 1180, 1190, 1216, 1265, 1368, 1416, 1434, 1486, 1491, 1613, 1692, 1740, 1758, 1897, 1946, 2005, 2023, 2066, 2277, 2354, 2403, 2462, 2480, 2523, 2561, 3646, 4033], '163:': [794, 2281], '215-222Crossref': [795, 2282], '(184)': [798, 2285], '12Desai': [801], 'Rybina': [803], 'Muller-Reichert': [805], 'Shevchenko': [807, 1181], 'Hyman': [809], '2421-2435Crossref': [817], '(185)': [820], 'Thus,': [823, 1776], 'might': [826, 1780, 2308, 2338, 4340], 'be': [827, 1056, 1781, 2339, 2609, 4298, 4342], 'assembled': [828], 'building': [830], 'blocks': [831], 'structurally': [834], 'related': [835], 'cerevisiae.': [840], 'By': [841], 'electron': [842], 'microscopy': [843], 'vertebrate': [845], 'appears': [847], 'multilayered': [850], 'structure': [851, 877], 'inner': [854], 'core': [855], 'embedded': [856, 1670], 'connected': [862], '40-nm': [865, 870], 'fibrous': [866, 881], 'gap': [867], 'thick': [871], 'plate.': [873], 'A': [874, 3112, 3230], '150-Å-thick': [875], 'dynamic': [876, 1048], 'corona': [882, 1845, 2126], 'extends': [883], 'outward': [884], '(13McEwen': [889], 'B.F.': [890, 954, 1027, 1440, 1764, 2029, 2486], 'Heagle': [891, 1028], 'A.B.': [892, 1029], 'Cassels': [893, 1030], 'G.O.': [894, 1031], 'Buttle': [895, 1032], 'Rieder': [897, 959, 1034], 'C.L.': [898, 928, 960, 1035], '1997;': [902, 919, 981, 1039, 1317], '137:': [903, 1040], '1567-1580Crossref': [904, 1041], '(176)': [907, 1044], '14Yao': [910], 'X.': [911], 'K.L.': [913, 1363, 1687], 'Cleveland': [914], '139:': [920], '435-447Crossref': [921], '(189)': [924], '15Rieder': [927], 'Int.': [929], 'Cytol.': [931], '1982;': [932], '79:': [933], '1-58Crossref': [934], '(298)': [937], '16Jokelainen': [940], 'P.T.': [941, 1245, 1271, 1926, 1952, 2383, 2409], 'Ultrastruct.': [943], 'Res.': [944], '1967;': [945], '19-44Crossref': [947], '(133)': [950, 1284, 1965, 2422], '17McEwen': [953], 'Arena': [955], 'J.T.': [956], 'Frank': [957], '1993;': [964], '120:': [965], '301-312Crossref': [966], '(73)': [969], '18Cooke': [972], 'C.A.': [973, 1263, 1944, 2401], 'Schaar': [974], 'B.': [975, 1409, 1733, 1998, 2455], 'Yen': [976], 'T.J.': [977], 'Earnshaw': [978, 1069, 1489], 'W.C.': [979, 1070, 1490], 'Chromosoma.': [980, 2582], '106:': [982], '446-455Crossref': [983], '(130)': [986, 1403, 1727, 1992, 2449], 'Vertebrate': [989], 'bind': [991, 4067, 4262], 'multiple': [992], '(from': [994], '15': [995, 2747], '30)': [997], 'whose': [998, 2649], 'ends': [1000, 1354], 'terminate': [1001], 'within': [1002, 4011], '13McEwen': [1026], 'microtubule-kinetochore': [1051, 1108, 2147], 'interfaces': [1052, 1109], 'thought': [1054], 'responsible': [1057], 'movement': [1060], 'mitosis': [1062], '(19Maiato': [1063], 'Deluca': [1065, 1485], 'Salmon': [1067, 1389, 1414, 1437, 1487, 1713, 1738, 1761, 1978, 2003, 2026, 2435, 2460, 2483, 3575, 3644], 'E.D.': [1068, 1390, 1415, 1438, 1488, 1714, 1739, 1762, 1979, 2004, 2027, 2436, 2461, 2484, 3576, 3645], 'Sci.': [1073, 1493, 2068, 2525], '117:': [1075, 1495], '5461-5477Crossref': [1076, 1496], '(323)': [1079, 1499], '20Kline-Smith': [1082, 1503], 'S.L.': [1083, 1504], 'Sandall': [1084, 1505], 'Curr.': [1088, 1272, 1391, 1509, 1715, 1953, 1980, 2410, 2437, 3579], 'Opin.': [1089, 1510], '2005;': [1092, 1444, 1513, 1768, 2033, 2490, 3581], '35-46Crossref': [1094, 1515], '(89)': [1097, 1518], 'Although': [1100], 'mechanisms': [1102], 'leading': [1103], 'formation': [1106, 1785, 2616], 'remain': [1110], 'poorly': [1111], 'understood,': [1112], 'protein': [1115, 1591, 2165, 3054, 3680, 3968, 4094], 'has': [1116, 1522], 'emerged': [1117], 'potentially': [1120], 'player': [1122], 'process.': [1125], 'In': [1126, 2098, 3983, 4054, 4221], 'budding': [1127], 'part': [1131, 2160], 'which': [1136, 1297, 1810, 3136, 3219, 4307], 'also': [1137, 2167, 2309, 3032, 3254, 4174, 4286, 4341], 'Spc25': [1142, 1551, 4049, 4259, 4321], '(10De': [1143], '21Wigge': [1160], 'P.A.': [1161], '2001;': [1167, 1191, 1371, 1695, 2583], '152:': [1168], '349-360Crossref': [1169], '(274)': [1172], '22Janke': [1175], 'Ortiz': [1177], 'Lechner': [1179], 'Magiera': [1183], 'M.M.': [1184], 'Schramm': [1185], 'Schiebel': [1187], 'E.': [1188, 2793], 'EMBO': [1189], '20:': [1192], '777-791Crossref': [1193], '(157)': [1196], 'This': [1199, 4125, 4172], 'quaternary': [1200, 2647, 2945, 2960, 3064], 'Xenopus': [1206], 'complexes': [1209, 2607], '(23Bharadwaj': [1210, 1607, 1891, 2348], 'R.': [1211, 1608, 1892, 2349], 'Qi': [1212, 1609, 1893, 2350], 'W.': [1213, 1313, 1610, 1894, 2351], 'Yu': [1214, 1611, 1895, 2352], 'Chem.': [1218, 1615, 1899, 2356], '279:': [1220, 1617, 1901, 2358], '13076-13085Abstract': [1221, 1618, 1902, 2359], '(97)': [1229, 1626, 1910, 2367], '24McCleland': [1232, 1913, 2370], 'M.L.': [1233, 1257, 1914, 1938, 2371, 2395], 'Gardner': [1234, 1915, 2372], 'R.D.': [1235, 1916, 2373], 'Kallio': [1236, 1258, 1917, 1939, 2374, 2396], 'M.J.': [1237, 1259, 1918, 1940, 2375, 2397], 'Daum': [1238, 1919, 2376], 'Gorbsky': [1240, 1268, 1921, 1949, 2378, 2406], 'G.J.': [1241, 1269, 1922, 1950, 2379, 2407], 'Burke': [1242, 1923, 2380], 'Stukenberg': [1244, 1270, 1925, 1951, 2382, 2408], '101-114Crossref': [1250, 1931, 2388], '(213)': [1253, 1934, 2391], '25McCleland': [1256, 1937, 2394], 'Barrett-Wilt': [1260, 1941, 2398], 'G.A.': [1261, 1942, 2399], 'Kestner': [1262, 1943, 2400], 'Shabanowitz': [1264, 1945, 2402], 'Hunt': [1266, 1947, 2404], 'D.F.': [1267, 1948, 2405], '131-137Abstract': [1276, 1957, 2414], 'referred': [1293], 'Hec1,': [1296, 4046, 4215], 'stands': [1298], 'highly': [1300], 'enhanced': [1301], 'cancer': [1303], '1': [1305, 2773, 2777, 2886, 2890, 3001, 3004, 3174, 3177], '(26Chen': [1306], 'Riley': [1308], 'D.': [1309, 3562], 'Chen': [1310], 'Lee': [1312], '6049-6056Crossref': [1319], '(115)': [1322], 'Scholar),': [1324, 3321], 'will': [1327, 2608], 'refer': [1328], 'Hec1': [1330, 1341, 1549, 1652, 2668, 2679, 2697, 3689, 3762, 4092, 4231], 'subunit': [1333], 'Antibodies': [1339], 'localize': [1342], 'where': [1351, 3322], 'anchored': [1359], '(27Howe': [1360, 1684], 'McDonald': [1362, 1686], 'Albertson': [1364, 1688], 'Meyer': [1366, 1690], 'B.J.': [1367, 1382, 1691, 1706, 1971, 2428, 3637], '153:': [1372, 1696], '1227-1238Crossref': [1373, 1697], '(102)': [1376, 1700, 2588], '28DeLuca': [1379, 1703, 1968, 2425], 'J.G.': [1380, 1407, 1428, 1704, 1731, 1752, 1969, 1996, 2017, 2426, 2453, 2474], 'Howell': [1381, 1705, 1970, 2427], 'Canman': [1383, 1707, 1972, 2429, 3563], 'J.C.': [1384, 1708, 1973, 2430, 3564], 'Hickey': [1385, 1410, 1435, 1709, 1734, 1759, 1974, 1999, 2024, 2431, 2456, 2481], 'J.M.': [1386, 1411, 1436, 1710, 1735, 1760, 1975, 2000, 2025, 2432, 2457, 2482], 'Fang': [1387, 1711, 1976, 2433, 3640], '13:': [1394, 1718, 1983, 2440], '2103-2109Abstract': [1395, 1719, 1984, 2441], '29DeLuca': [1406, 1730, 1995, 2452], 'Moree': [1408, 1732, 1997, 2454], '159:': [1420, 1744, 2009, 2466], '549-555Crossref': [1421, 1745, 2010, 2467], '(229)': [1424, 1748, 2013, 2470], '30DeLuca': [1427, 1751, 2016, 2473], 'Dong': [1429, 1753, 2018, 2475], 'Hergert': [1431, 1755, 2020, 2477], 'Strauss': [1433, 1757, 2022, 2479], 'McEwen': [1439, 1763, 2028, 2485], '16:': [1445, 1769, 2034, 2491], '519-531Crossref': [1446, 1770, 2035, 2492], '(204)': [1449, 1773, 2038, 2495], 'Depletion': [1452, 1547], 'or': [1453, 1550, 1580, 1653, 1832, 1842, 2123, 2297, 3519, 3530, 3687, 3737, 3957, 3969, 4081, 4119, 4145, 4166, 4268, 4274], 'inactivation': [1454, 2326], 'methods': [1463], 'invariably': [1464], 'results': [1465], 'severe': [1467, 4179, 4309], 'problems': [1468], 'congression': [1471, 1532], 'lack': [1473], 'poleward': [1475], 'forces': [1476], '(reviewed': [1480], 'Refs.': [1482], '19Maiato': [1483], 'Scholar': [1501], 'There': [1521], 'been': [1523], 'some': [1524], 'controversy': [1525], 'whether': [1528], 'defective': [1530], 'phenotype': [1533, 2347], 'resulting': [1534, 3266], 'depletion': [1536, 1831, 2315, 2346], 'caused': [1540, 4301, 4345], 'defect': [1543], 'attachment.': [1546], 'RNA': [1553], 'interference': [1554], 'resulted': [1558], 'failure': [1561, 1679], 'congress': [1565], 'equator,': [1569], 'but': [1570, 4295], 'existence': [1572], 'connections': [1575], 'resisted': [1577], 'short': [1578], 'cold': [1579], 'calcium': [1581], 'treatments': [1582], 'end-binding': [1590], 'EB1': [1592], 'interface': [1596, 2111, 2148], 'suggested': [1597], 'may': [1602, 4297], 'form': [1603, 3996], '31Martin-Lluesma': [1629, 2041, 2498], 'Stucke': [1631, 2043, 2500], 'V.M.': [1632, 2044, 2080, 2501, 2537], 'Nigg': [1633, 2045, 2502], 'E.A.': [1634, 2046, 2503], 'Science.': [1635, 2047, 2504, 4034], '297:': [1637, 2049, 2506], '2267-2270Crossref': [1638, 2050, 2507], '(377)': [1641, 2053, 2510], 'studies,': [1645], 'however,': [1646], 'concluded': [1647], 'depleted': [1650], 'Nuf2': [1654, 4071, 4097, 4135, 4213, 4233], 'display': [1655], 'damaged': [1657], 'kinetochore,': [1659], 'coupled': [1660], 'dramatic': [1663], 'decrease': [1664], 'number': [1667, 3331], 'surface': [1675], 'accumulate': [1681], 'cold-stable': [1682], 'interactions,': [1789], 'suggestive': [1790], 'direct': [1793, 2105, 2296], 'binding': [1797, 3026, 4161, 4205], 'or,': [1798], 'alternatively,': [1799], 'pathway,': [1806], 'impairment': [1808], 'abrogates': [1811], 'recruitment': [1812], 'other': [1814, 2173], 'binding.': [1819], 'Indeed,': [1820], 'depletion/inactivation': [1822], 'causes': [1827], 'corresponding': [1829], 'reduction': [1833], 'group': [1836], 'normally': [1839], 'localized': [1840], 'peripheral': [1846, 1870, 2127], 'plate,': [1851, 2132], 'including': [1852], 'motor': [1854, 1874], 'dynein/dynactin': [1856], 'checkpoint': [1861, 1871], 'Mps1,': [1863, 2302], 'Mad1,': [1864, 2303], 'Mad2,': [1865, 2304], 'Zw10,': [1866, 2305], 'Rod.': [1868], 'However,': [1869, 4351], 'such': [1876, 2175], 'Bub1,': [1878], 'BubR1,': [1879], 'CENP-E': [1881], 'retain': [1882], 'essentially': [1884, 3058], 'under': [1888], 'conditions': [1890], '32Hori': [2056, 2513], 'Haraguchi': [2058, 2515, 2578], 'Hiraoka': [2060, 2517, 2580], 'Kimura': [2062, 2519], 'Fukagawa': [2064, 2521], '116:': [2070, 2527], '3347-3362Crossref': [2071, 2528], '(125)': [2074, 2531], '33Meraldi': [2077, 2534], 'Draviam': [2079, 2536], '7:': [2086, 2543], '45-60Abstract': [2087, 2544], '(393)': [2095, 2552], 'summary,': [2099, 4222], 'stabilizing': [2108], 'controlling': [2114], 'subset': [2119], 'although': [2133], 'its': [2134, 4188], 'precise': [2135], 'function': [2136], 'processes': [2139], 'remains': [2140], 'unclear.': [2141], 'complexity': [2143, 2594], 'putative': [2146], 'underscored': [2150], 'recent': [2153], 'discovery': [2154], 'Mtw1-Mis12': [2170], 'Zwint-1': [2177], 'Spc105': [2179], '(7Obuse': [2180], 'indirect': [2298], 'receptor': [2300], 'Rod': [2307], 'explain': [2310], 'effects': [2312, 2322], 'range': [2323], 'complete': [2325], 'sustained': [2328], 'activation': [2329], 'followed': [2330], 'death,': [2333], 'discrepancy': [2336], 'explained': [2340, 4337], 'penetrance': [2343], '34Gillett': [2555], 'E.S.': [2556], 'Espelin': [2557], 'C.W.': [2558], '164:': [2565], '535-546Crossref': [2566], '(153)': [2569], '35Nabetani': [2572], 'Koujin': [2574], 'Tsutsumi': [2576], '110:': [2584], '322-334Crossref': [2585], 'Because': [2591, 3362], 'structure,': [2597], 'development': [2599], 'vitro': [2602], 'approaches': [2603], 'invaluable': [2610], 'elucidate': [2612], 'mechanism': [2614], 'interfaces.': [2619], 'Here,': [2620], 'describe': [2622], 'step': [2624], 'direction': [2627], 'represented': [2628], 'reconstitution': [2631], 'characterization': [2633], 'We': [2639, 4002], 'show': [2640], 'forms': [2645], 'biophysical,': [2650], 'biochemical,': [2651], 'biological': [2653], 'indistinguishable': [2656], 'Cloning,': [2663], 'Expression,': [2664], 'Purification': [2666], 'Complex': [2669], 'Constructs—': [2670], 'constructs': [2672, 2712, 4016], 'were': [2681, 2699, 2713, 2739, 2800, 2846, 2862, 3051, 3073, 3113, 3149, 3253, 3432, 3486, 3498, 3503, 3521, 3535, 3543, 3600, 3633, 3661, 3671, 3741, 3755, 3775, 3793, 3810, 3841, 3887, 3911, 3941, 4085], 'generated': [2682], 'PCR': [2684], 'fetal': [2688, 3610, 3713], 'thymus': [2689], 'cDNA': [2690], 'library': [2691], '(Invitrogen).': [2692], 'sequences': [2698], 'subcloned': [2700], 'modified': [2703, 3604], 'pGEX-6P': [2704], 'vector': [2705], '(pGEX-6P-2RBS)': [2706], 'support': [2708], 'dicistronic': [2709], 'expression.': [2710], 'sequenced.': [2714], 'Protein': [2715], 'expression': [2716, 3055], 'Escherichia': [2718], 'coli': [2719, 2794], 'strain': [2720], 'BL21': [2721], '(DE3)': [2722], 'was': [2723, 2730, 2896, 2922, 2950, 2962, 3013, 3083, 3142, 3191, 3211, 3220, 3272, 3287, 3384, 3409, 3455, 3465, 3861, 3872, 3963, 3979, 4076, 4098, 4162, 4241, 4358], 'induced': [2724], '400': [2726], 'μm': [2727, 3684, 3693], 'isopropyl-β-d-thiogalactopyranoside.': [2728], 'Expression': [2729], 'continued': [2731], '∼12–16': [2733], 'h': [2734, 2829, 2855, 2914, 3195, 3525], '18–20': [2736], '°C.': [2737, 2917, 3208], 'Cells': [2738, 3670], 'harvested': [2740], 'centrifugation': [2742, 2803], '4,000': [2744], 'rpm': [2745, 2806, 3198], 'min': [2748, 2809], 'Beckman': [2751, 3201], 'JLA': [2752], '10.5': [2753], 'rotor': [2754, 3205], 'resuspended': [2756], '30–40': [2758], 'ml': [2759], 'lysis': [2761, 2868], 'buffer': [2762, 2869, 2877, 2992, 3165, 3371], '(50': [2763, 2878, 2993, 3166], 'mm': [2764, 2769, 2774, 2778, 2879, 2884, 2887, 2891, 2994, 2999, 3002, 3005, 3078, 3167, 3172, 3175, 3178, 3182, 3710], 'Tris-HCl,': [2765, 2880, 3168], 'pH': [2766, 2881, 2996, 3169, 3704], '7.6,': [2767, 2882, 2997, 3170], '600': [2768, 2998], 'NaCl,': [2770, 2885, 3000, 3173], '10%': [2771, 3609, 3712], 'glycerol,': [2772], 'EDTA,': [2775, 2888, 3003, 3179], 'dithiothreitol': [2779], 'supplemented': [2780, 3707], 'Complete': [2782], 'protease': [2783, 2905, 2949], 'inhibitor': [2784], 'mixture': [2785], 'Roche': [2787], 'Molecular': [2788], 'Biochemicals)': [2789], 'per': [2790, 2908, 3021], 'liter': [2791, 3022], 'culture.': [2795], 'After': [2796, 2853, 2942, 3867], 'sonication,': [2797], 'lysates': [2799, 4084], 'cleared': [2801], '40,000': [2805, 3197], '45–60': [2808], '55.1': [2812], 'Ti': [2813], 'rotor.': [2814], 'Glutathione': [2815], 'S-transferase': [2816], '(GST)': [2817], '1The': [2818], 'abbreviations': [2819], 'used': [2820, 3052, 3273, 3792, 3934], 'are:': [2821], 'GST,': [2822, 4079, 4143], 'glutathione': [2823], 'S-transferase;': [2824], 'Alexa,': [2825], 'Alexa': [2826, 3476], 'Fluor': [2827, 3477], '488;': [2828], '(prefix),': [2830], 'human;': [2831], 'SEC,': [2832], 'size': [2833, 2988], 'exclusion': [2834, 2989], 'chromatography;': [2835], 'SFM,': [2836], 'scanning': [2837], 'force': [2838], 'microscopy;': [2839], 'WB,': [2840], 'Western': [2841, 3460], 'blotting.': [2842], '-Spc24-Spc25': [2843], 'GST-Nuf2-Hec1': [2845, 2920], 'purified': [2847, 2925, 3435], 'glutathione-agarose': [2849], 'beads': [2850, 2861], '(Amersham': [2851, 2906, 3089, 3098], 'Biosciences).': [2852, 3099], '2–3': [2854], 'agitation': [2857], '4': [2859, 2916, 3207, 3759], '°C,': [2860, 3621], 'washed': [2863, 3873], '30': [2865, 2873], 'volumes': [2866, 2874, 3101], 'equilibrated': [2871, 2986], 'cleavage': [2876, 2937], '150': [2883, 3171], 'dithiothreitol).': [2892], 'subcomplex': [2895, 4013], 'cleaved': [2897, 3865], 'GST': [2899, 2955, 3427, 3442, 4118, 4165], '10': [2901, 3181], 'units': [2902], 'PreScission': [2904, 2936, 2948], 'Biosciences)': [2907, 3090], 'milligram': [2909], 'substrate': [2911], '16': [2913], 'Simultaneously,': [2918], 'expressed': [2923, 3433, 3964], 'described': [2927, 3060, 3412, 3437, 3540, 3555, 3634, 3844, 3859], 'above': [2928, 3061, 3860], 'incubated': [2930, 3522, 3756, 3776, 4086], 'flow': [2933, 2966], 'through': [2934], 'GST-Spc24-Spc25': [2940], 'forming': [2943], 'added': [2951], 'remove': [2953], 'moiety': [2956], 'Nuf2.': [2958], 'collected': [2963], 'through,': [2967], 'concentrated': [2968], 'up': [2969], '5': [2971], 'mg/ml': [2972], 'Vivaspin': [2974], '(molecular': [2975], 'weight': [2976, 3286, 3304], 'cut-off': [2977], '30,000)': [2978], 'loaded': [2980, 3150], 'onto': [2981, 3152], 'Superose': [2983], '6': [2984, 4194], 'column': [2985, 3097], 'chromatography': [2990], '(SEC)': [2991], 'Tris-HCl': [2995], 'dithiothreitol,': [3006, 3176], '5%': [3008, 3622], 'glycerol).': [3009], 'final': [3011], 'yield': [3012], '∼0.1': [3016], 'mg': [3017], 'pure': [3019], 'bacteria.': [3024], 'For': [3025, 3144, 3657, 3830], 'assays': [3027], 'analytical': [3029], 'SEC': [3030, 3082, 3494, 3538], 'created': [3033], 'vectors': [3035, 3050, 3940], 'pGEX-6P-Nuf2-(1–137),': [3036], 'pGEX-6P-Nuf2-(1–386),': [3037], 'pGEX-6P-Nuf2-(387–464),': [3038], 'pAM3-His6-Hec1-(1–230),': [3039], 'pAM3-His6-Hec1-(1–445),': [3040], 'pAM3-His6-Hec1-(446–642),': [3041], 'pGEX-6P-2RBS-Spc24-(1–131)·Spc25-(1–144),': [3042], 'pGEX-6P-2RBS-hSpc24-(1–131)·hSpc25-(145–224),': [3043], 'pGEX-6P-2RBS-hSpc24-(132–197)·hSpc25-(1–144),': [3044], 'pGEX-6P-2RBS-hSpc24-(132–197)·hSpc25-(145–224),': [3045], 'pGEX-6P-2RBS-GST-Nuf2-(1–386)·Hec1-(1–445),': [3046], 'pGEX-6P-2RBS-GST-Nuf2-(387–464)·Hec1-(446–642).': [3048], 'purification': [3057], 'Ndc80,': [3065], 'except': [3066], 'salt': [3069], 'concentrations': [3070], 'buffers': [3072], 'usually': [3074], 'kept': [3075], '300': [3077], 'NaCl.': [3079], 'Hydrodynamic': [3080], 'Analysis—Analytical': [3081], 'performed': [3084], 'SMART': [3087], 'system': [3088], 'Superdex': [3093], '200': [3094, 3491], 'PC': [3095, 3492], '3.2/30': [3096, 3493], 'Elution': [3100], 'bovine': [3103, 3235, 3611, 3714], 'thyroglobulin,': [3104], 'rabbit': [3105], 'aldolase,': [3106], 'hen': [3107], 'egg': [3108], 'albumin,': [3109], 'ribonuclease': [3111], 'plotted': [3114, 3259], 'against': [3115, 3260, 3394, 4323], 'Stokes': [3118, 3138, 3291], 'radii': [3119], '(82.55,': [3120], '46.15,': [3121], '30.5,': [3122], '16.4': [3124], 'Å,': [3125], 'respectively)': [3126], 'generate': [3128], 'calibration': [3130, 3267], 'curve': [3131, 3268], '(R2': [3132, 3269], '=': [3133, 3270, 3305, 3324], '0.98)': [3134], 'radius': [3139, 3292], 'determined.': [3143], 'glycerol': [3145, 3155, 3188], 'velocity': [3146], 'gradients,': [3147], 'samples': [3148], 'directly': [3151], '12-ml': [3153], '5–40%': [3154], 'gradients': [3156], 'prepared': [3157], 'layering': [3159], 'eight': [3160], '1.5-ml': [3161], 'aliquots': [3162], 'gradient': [3164, 3210], 'phenylmethylsulfonyl': [3183], 'fluoride)': [3184], 'decreasing': [3187], 'concentration.': [3189], 'Centrifugation': [3190], 'protracted': [3192], '48': [3194], 'SW41Ti': [3202], 'swinging': [3203], 'bucket': [3204], 'fractionated': [3212], '300-μl': [3214], 'fractions,': [3215], 'content': [3217], 'visualized': [3221], 'SDS-PAGE': [3223, 3506], 'after': [3224], 'precipitation': [3225], 'trichloroacetic': [3227], 'acid.': [3228], 'Chymotrypsinogen': [3229], '(25-kDa': [3231], 'monomer,': [3232, 3239], '2.58': [3233], 'S),': [3234, 3241, 3246], 'serum': [3236, 3612, 3779], 'albumin': [3237], '(60-kDa': [3238], '4.22': [3240], 'aldolase': [3242], '(158-kDa': [3243], 'tetramer,': [3244, 3250], '7.4': [3245], 'catalase': [3248], '(240-kDa': [3249], '11.3': [3251], 'S)': [3252], 'analyzed': [3255, 3359, 3487, 3504, 3536], 'elution': [3258], 'Svedberg': [3263], 'coefficient.': [3264], '0.97)': [3271], 'determine': [3275], 'sedimentation': [3277], 'coefficient': [3278], '(s)': [3279], 'molecular': [3285, 3303, 3389], 'calculated': [3288, 3410], '(a)': [3293], 'Ångstroms': [3295], 's': [3298, 3398, 3869], 'according': [3299, 3368], 'equation': [3302], 'αas': [3306], '(36Siegel': [3307], 'L.M.': [3308], 'Monty': [3309], 'K.J.': [3310], 'Biochim.': [3311], 'Biophys.': [3312], 'Acta.': [3313], '1966;': [3314], '346-362Crossref': [3316], '(1547)': [3319], 'α': [3323, 3382], '(6πη0N)/(1': [3325], '-': [3326], 'νρ),': [3327], 'N': [3328], "Avogadro's": [3330], '(6.02': [3332], '×': [3333, 3397], '1023),': [3334], 'η0': [3335, 3363], 'viscosity': [3338], 'medium': [3340, 3348, 3606], '(g/(cm·s)),': [3341], 'ρ': [3342, 3365], 'density': [3345], '(g/ml),': [3349], 'ν': [3351, 3374], 'partial': [3354], 'specific': [3355], 'volume': [3356, 3676], 'particle': [3360], '(ml/g).': [3361], 'values': [3366], 'change': [3367], 'composition': [3372], 'depends': [3375], 'amino': [3378], 'acidic': [3379], 'composition,': [3380], 'value': [3383], 'determined': [3385, 3991], 'plotting': [3387], 'weights': [3390], 'standard': [3392], 'values.': [3399], 'axial': [3401], 'ratio': [3402], 'prolate': [3405], 'ellipsoid': [3406], 'rotation': [3408], '(37Harding': [3413], 'S.E.': [3414], 'Colfen': [3415], 'Anal.': [3417], 'Biochem.': [3418], '1995;': [3419], '228:': [3420], '131-142Crossref': [3421], '(49)': [3424], 'Pull-down': [3428], 'Experiments—GST': [3429], 'fusion': [3430, 3443, 3952, 3974, 4093], 'above.': [3438, 3541, 3845], 'Binding': [3439, 3473], 'interacting': [3447], 'partners': [3448], 'fused': [3449, 3980, 4072], 'hexahistidine': [3452], '(His6)': [3453], 'tag': [3454, 3459, 3956, 3973], 'tested': [3456, 4061, 4247, 4314], 'anti-His': [3458], 'blotting': [3461], '(WB).': [3462], 'Each': [3463], 'verified': [3466], 'three': [3470], 'independent': [3471], 'experiments.': [3472], 'Assay': [3474], '488': [3478], '(Alexa)': [3479], 'Labeling—Hec1-(1–445)·Nuf2-(1–386),': [3480], 'Hec1-(446–642)·Nuf2-(387–464),': [3481], 'Alexa-Spc24-(1–197)·Spc25-(1–224),': [3482], 'Alexa-Spc24-(70–197)·Spc25-(57–224)': [3484], 'Superdex™': [3490], 'column.': [3495], '35-μl': [3496], 'fractions': [3497], 'collected,': [3499], 'contents': [3502], 'Coomassie': [3508], 'staining.': [3509], 'test': [3511], 'Hec1-(1–445)·Nuf2-(1–386)': [3518], 'Hec1-(446–642)·Nuf2-(387–464)': [3520], '2': [3524], 'ice': [3527], 'Alexa-Spc24-(1–197)·Spc25-(1–224)': [3529], 'Alexa-Spc24-(70–197)·Spc25-(57–224).': [3531], 'reaction': [3533], 'mixes': [3534], 'Proteins': [3542], 'labeled': [3544], 'Alexa-488': [3546], 'succimidyl': [3547], 'ester': [3548], 'reactive': [3549], 'dye': [3550], '(Molecular': [3551], 'Probes,': [3552], 'Inc.)': [3553], '(38De': [3556], 'Antoni': [3557], 'Pearson': [3559], 'C.G.': [3560], 'Cimini': [3561], 'Sala': [3565], 'V.': [3566], 'Nezi': [3567], 'L.': [3568, 3572], 'Mapelli': [3569], 'Sironi': [3571], 'Faretta': [3573], 'Musacchio': [3577], '15:': [3582], '214-225Abstract': [3583], '(335)': [3591], 'Culture,': [3595], 'Injections,': [3596], 'Microscopy—HeLa': [3598], 'maintained': [3601], "Dulbecco's": [3603], "Eagle's": [3605], '(Invitrogen)': [3607], 'antibiotic/antimycotic': [3614, 3717], 'solution': [3615], 'cultured': [3617], '37': [3620], 'CO2': [3623], 'incubator.': [3624], 'culture': [3626], 'PtK1': [3628], 'microinjection': [3631, 3658], 'techniques': [3632], 'previously': [3635], '(39Howell': [3636], 'Hoffman': [3638], 'D.B.': [3639], 'Murray': [3642], 'A.W.': [3643], '2000;': [3649], '150:': [3650], '1233-1250Crossref': [3651], '(283)': [3654], 'experiments,': [3659], 'grown': [3662], 'acid-washed': [3664], 'coverslips': [3665, 3836], '35-mm': [3667], 'Petri': [3668], 'dishes.': [3669], 'microinjected': [3672], '∼1–5%': [3674], 'Alexa-labeled': [3678, 3688], '(at': [3682, 3691], '86': [3683], 'needle': [3685, 3694], 'concentration)': [3686, 3695], '26': [3692], 'then': [3697], 'placed': [3698], "Leibovitz's": [3700], 'dye-free': [3701], 'L-15': [3702], 'medium,': [3703], '7.2': [3705], '(Sigma),': [3706], '7': [3709], 'Hepes,': [3711], 'serum,': [3715], 'solution,': [3718], '4.5': [3719], 'g/liter': [3720], 'glucose,': [3721], '0.45': [3723], 'units/ml': [3724], 'Oxyrase': [3725], '(Oxyrase': [3726], 'Inc.,': [3727, 3781], 'Mansfield,': [3728], 'OH).': [3729], 'injected': [3732, 3799], 'Alexa-Spc24-Spc25': [3735, 3798], 'Alexa-Ndc80': [3739], 'fixed': [3742], '4%': [3744], 'paraformaldehyde': [3745], 'subsequently': [3747], 'permeabilized': [3748], '1%': [3750], 'Triton': [3751], 'X-100.': [3752], 'Alexa-Spc24–25-injected': [3753], 'overnight': [3757], '°C': [3760], 'primary': [3763], 'antibodies': [3764, 3791], '(Abcam,': [3765], 'Cambridge,': [3766], 'UK)': [3767], 'dilution': [3770, 3786], '1:750;': [3772], 'Alexa-Ndc80-injected': [3773], 'CREST': [3778], '(Antibodies': [3780], 'Davis,': [3782], 'CA)': [3783, 3897], '1:2000.': [3788], 'secondary': [3790], 'rhodamine': [3794, 3802], 'Red-X': [3795, 3803], 'anti-mouse': [3796], 'anti-human': [3804], 'complex-injected': [3807], 'cells.': [3808], 'Images': [3809, 3886], 'obtained': [3811, 3888], 'Nikon': [3814], '100×,': [3815], '1.4': [3816], 'numerical': [3817], 'aperture': [3818], 'planapochromat': [3819], 'oil': [3820], 'immersion': [3821], 'lens': [3822], 'spinning': [3825], 'disc': [3826], 'confocal': [3827], 'fluorescence': [3828], 'microscope.': [3829], 'live': [3831], 'imaging,': [3833, 3849], 'mounted': [3837], 'Rose': [3839], 'chambers': [3840], 'imaged': [3842], 'Scanning': [3846], 'Force': [3847], 'Microscopy—For': [3848], '20': [3850], 'μl': [3851], 'diluted': [3854], 'deposited': [3862], 'freshly': [3864], 'mica.': [3866], '∼30': [3868], 'mica': [3871], 'water': [3875], '(glass-distilled;': [3876], 'Sigma)': [3877], 'exposed': [3879], 'stream': [3882], 'filtered': [3884], 'air.': [3885], 'NanoScope': [3891], 'IV': [3892], '(Digital': [3893], 'Instruments;': [3894], 'Santa': [3895], 'Barbara,': [3896], 'operating': [3898], 'tapping': [3900], 'mode': [3901], 'air': [3903], 'type': [3906], 'E': [3907], 'scanner.': [3908], 'Silicon': [3909], 'nanotips': [3910], 'Digital': [3913], 'Instruments': [3914], '(Nanoprobes).': [3915], 'Domain': [3916], 'Structure': [3917], 'Complex—To': [3922], 'investigate': [3923], 'connecting': [3926], 'bacterial': [3936], 'co-expression': [3937, 3984, 4363], 'strategy.': [3938], 'Dicistronic': [3939], 'designed': [3942], 'express': [3944], 'first': [3946, 3977, 4056], 'cistron': [3948, 3962, 3978], 'C-terminal': [3951, 4269, 4317], 'His6': [3955, 3972], 'GST.': [3959, 4074], 'second': [3961], 'untagged': [3967], '(when': [3975], 'GST).': [3982], 'experiments': [3985, 4059], 'full-length': [3988], 'Nuf2-Hec1': [3993], 'two': [3997], '(see': [4000], 'below).': [4001], 'exploited': [4003], 'observation': [4005], 'map': [4007], 'dimerization': [4009], 'creating': [4015], 'expected': [4017], 'coincide': [4019], '(40Lupas': [4027], 'Van': [4029], 'Dyke': [4030], 'Stock': [4032], '1991;': [4035], '252:': [4036], '1162-1164Crossref': [4037], '(3483)': [4040], 'Scholar)': [4042], 'regions': [4043, 4069, 4133, 4270], 'shown': [4050], 'Fig.': [4052], '1A.': [4053], 'set': [4057], 'ability': [4063, 4249], 'His-Hec1-(1–445)': [4065, 4075, 4106, 4191], 'co-expressed': [4077, 4140], 'GST-Nuf2-(1–385),': [4080, 4144], 'GST-hNuf2-(386–464).': [4082, 4146], 'Bacterial': [4083], 'GSH-Sepharose,': [4088], 'any': [4090], 'His-tagged': [4091, 4252], 'bound': [4095, 4107, 4277, 4287, 4328], 'detected': [4099], 'WB': [4101], 'anti-His-tagged': [4104], 'antibody.': [4105], 'tightly': [4108, 4278], 'GST-Nuf2-(1–385)': [4110, 4167, 4177], '(Fig.': [4111, 4281, 4291, 4332], '1B,': [4112], 'lane': [4113, 4283, 4293, 4334], '6)': [4114], 'not': [4116], 'GST-hNuf2-(386–464)': [4120, 4156], '(lanes': [4121, 4168], '3': [4122, 4169], '9).': [4124, 4335], 'result': [4126], 'identifies': [4127], 'between': [4130, 4153, 4206], 'Hec1.': [4137], 'Next,': [4138, 4312], 'His-Hec1-(446–642)': [4141, 4154], 'Interestingly,': [4147], 'found': [4149], '(lane': [4157], '9),': [4158, 4294], 'whereas': [4159], 'no': [4160], 'observed': [4163], '6).': [4171, 4284], 'experiment': [4173], 'indicated': [4175], 'undergoes': [4178, 4308], 'proteolytic': [4180, 4310], 'degradation,': [4181], '(compare': [4192], 'lanes': [4193], 'panels': [4196], 'B': [4197], 'C).': [4199], 'Finally,': [4200], 'failed': [4202], 'detect': [4204], 'heads': [4211], 'GST-Nuf2-(1–137)': [4216], 'His-Hec1-(1–230)': [4218], '(not': [4219], 'shown).': [4220], 'entire': [4224], 'length': [4225], 'coils': [4229], 'engaged': [4235], 'dimerization.': [4237], 'same': [4239], 'approach': [4240], 'applied': [4242], 'Spc24-Spc25.': [4244], 'First,': [4245], 'version': [4253], 'region': [4257], '(His-Spc25-(1–144))': [4260], 'GST-tagged': [4263], 'versions': [4264], 'N-': [4267], 'Spc24': [4272], '(GST-Spc24-(1–131)': [4273], 'GST-Spc24-(132–197)).': [4275], 'His-Spc25-(1–144)': [4276, 4285], 'GST-Spc24-(1–131)': [4280, 4324], '1D,': [4282, 4292], 'reproducibly': [4288], 'GST-Spc24-(132–197)': [4290, 4331, 4357], 'artifact': [4300, 4344], 'instability': [4304, 4348], 'GST-Spc24-(132–197),': [4306], 'degradation.': [4311], 'domain': [4319], '(His-Spc25-(145–224))': [4322], 'GST-Spc24-(132–197).': [4326, 4350], 'His-Spc25-(145–224)': [4327], 'weakly': [4329], '1E,': [4333], 'As': [4336], 'above,': [4338], 'fraction': [4355], 'protected': [4359], 'degradation': [4361], 'wi': [4364]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2168277561', 'counts_by_year': [{'year': 2024, 'cited_by_count': 4}, {'year': 2023, 'cited_by_count': 5}, {'year': 2022, 'cited_by_count': 8}, {'year': 2021, 'cited_by_count': 4}, {'year': 2020, 'cited_by_count': 5}, {'year': 2019, 'cited_by_count': 10}, {'year': 2018, 'cited_by_count': 7}, {'year': 2017, 'cited_by_count': 10}, {'year': 2016, 'cited_by_count': 10}, {'year': 2015, 'cited_by_count': 8}, {'year': 2014, 'cited_by_count': 9}, {'year': 2013, 'cited_by_count': 7}, {'year': 2012, 'cited_by_count': 13}], 'updated_date': '2024-12-10T00:40:30.009587', 'created_date': '2016-06-24'}