Title: Cytotoxic and anti-inflammatory activity of some Thai medicinal plants
Abstract:In order to search for new anti-inflammatory agent for use as an adjunctive treatment for periodontal diseases, we have studied crude extracts from plants of family Rutaceae, Asteraceae and Maliaceae ...In order to search for new anti-inflammatory agent for use as an adjunctive treatment for periodontal diseases, we have studied crude extracts from plants of family Rutaceae, Asteraceae and Maliaceae which have been claimed to possess anti-microbial and anti-inflammatory activities. The cytotoxic effect of these plant crude extracts on human gingival fibroblasts (HGFs) and monocytes was determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Then, a non-cytotoxic dose was chosen for each plant extract and used to determine the anti-inflammatory activity. The enzyme linked to immunosorbent assay was used to assay tumor necrosis factor-alpha (TNF-a) and interleukin-1beta (IL-1b) released from cells treated with bacterial lipopolysaccharides (LPS). The results showed that the half maximal inhibitory concentration (IC50) of plant extracts for HGFs were in the range of 308.12 to 1168.66 µg/ml while the IC50 for macrophages were in the range of 54.6 to 90.92 µg/ml. Stem bark of Cratoxylum formosumdemonstrated a comparable inhibitory activity to dexamethasone (DM) on TNF-a production by both cells, but showed detectable reduction of IL-1b only in macrophages. Other plant extracts showed no significant difference for reduction of inflammatory cytokines from macrophages. Moreover, leaf and branch of Murraya paniculata showed greater inhibitory activity than DM on the production of TNF-a from stimulated HGFs. Therefore, these plant extracts may be good candidates for further drug development for adjuctive treatment of periodontal diseases.
Key words: Cytotoxic effect, anti-inflammatory activity, Rutaceae, Asteraceae, Maliaceae.Read More
Publication Year: 2012
Publication Date: 2012-06-21
Language: en
Type: article
Indexed In: ['crossref']
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Cited By Count: 7
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