Title: Research Note: Simple molecular discrimination of cultivated <i>Porphyra</i> species (<i>Porphyra yezoensis</i> and <i>Porphyra tenera</i>) and related wild species (Bangiales, Rhodophyta)
Abstract:SUMMARY To discriminate between cultivated Porphyra species ( Porphyra yezoensi s and Porphyra tenera ) and closely related wild Porphyra species, we developed a polymerase chain reaction‐restriction ...SUMMARY To discriminate between cultivated Porphyra species ( Porphyra yezoensi s and Porphyra tenera ) and closely related wild Porphyra species, we developed a polymerase chain reaction‐restriction fragment length polymorphism (PCR‐RFLP) analysis of the rbc L gene using five restriction enzymes. Although our previous PCR‐RFLP analyses of internal transcribed spacer (ITS) rDNA and plastid RuBisCO spacer regions could not always discriminate wild P. yezoensis , wild P. tenera , and closely related wild species, the PCR‐RFLP profiles of the rbc L gene were useful in discriminating samples collected from natural habitats. Therefore, PCR‐RFLP analysis of the rbc L gene will help in the simple identification of a large number of samples, not only for the establishment of reliable cultures as breeding material, but also for the taxonomic investigations of species that are closely related to cultivated Porphyra .Read More
Publication Year: 2009
Publication Date: 2009-12-01
Language: en
Type: article
Indexed In: ['crossref']
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Cited By Count: 8
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