Get quick answers to your questions about the article from our AI researcher chatbot
{'id': 'https://openalex.org/W2163119877', 'doi': 'https://doi.org/10.1085/jgp.107.2.195', 'title': 'Identification of channel-lining residues in the M2 membrane-spanning segment of the GABA(A) receptor alpha1 subunit.', 'display_name': 'Identification of channel-lining residues in the M2 membrane-spanning segment of the GABA(A) receptor alpha1 subunit.', 'publication_year': 1996, 'publication_date': '1996-02-01', 'ids': {'openalex': 'https://openalex.org/W2163119877', 'doi': 'https://doi.org/10.1085/jgp.107.2.195', 'mag': '2163119877', 'pmid': 'https://pubmed.ncbi.nlm.nih.gov/8833341', 'pmcid': 'https://www.ncbi.nlm.nih.gov/pmc/articles/2219269'}, 'language': 'en', 'primary_location': {'is_oa': True, 'landing_page_url': 'https://doi.org/10.1085/jgp.107.2.195', 'pdf_url': 'https://rupress.org/jgp/article-pdf/107/2/195/1143778/195.pdf', 'source': {'id': 'https://openalex.org/S168548653', 'display_name': 'The Journal of General Physiology', 'issn_l': '0022-1295', 'issn': ['0022-1295', '1540-7748'], 'is_oa': True, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310315676', 'host_organization_name': 'Rockefeller University Press', 'host_organization_lineage': ['https://openalex.org/P4310315676'], 'host_organization_lineage_names': ['Rockefeller University Press'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, 'type': 'article', 'type_crossref': 'journal-article', 'indexed_in': ['crossref', 'pubmed'], 'open_access': {'is_oa': True, 'oa_status': 'bronze', 'oa_url': 'https://rupress.org/jgp/article-pdf/107/2/195/1143778/195.pdf', 'any_repository_has_fulltext': True}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5012806956', 'display_name': 'Ming Xu', 'orcid': 'https://orcid.org/0000-0002-3996-4304'}, 'institutions': [{'id': 'https://openalex.org/I78577930', 'display_name': 'Columbia University', 'ror': 'https://ror.org/00hj8s172', 'country_code': 'US', 'type': 'education', 'lineage': ['https://openalex.org/I78577930']}], 'countries': ['US'], 'is_corresponding': False, 'raw_author_name': 'M Xu', 'raw_affiliation_strings': ['Center for Molecular Recognition, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA.'], 'affiliations': [{'raw_affiliation_string': 'Center for Molecular Recognition, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA.', 'institution_ids': ['https://openalex.org/I78577930']}]}, {'author_position': 'last', 'author': {'id': 'https://openalex.org/A5050960288', 'display_name': 'Myles H. Akabas', 'orcid': 'https://orcid.org/0000-0001-8781-7846'}, 'institutions': [{'id': 'https://openalex.org/I78577930', 'display_name': 'Columbia University', 'ror': 'https://ror.org/00hj8s172', 'country_code': 'US', 'type': 'education', 'lineage': ['https://openalex.org/I78577930']}], 'countries': ['US'], 'is_corresponding': False, 'raw_author_name': 'M H Akabas', 'raw_affiliation_strings': ['Center for Molecular Recognition, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA.'], 'affiliations': [{'raw_affiliation_string': 'Center for Molecular Recognition, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA.', 'institution_ids': ['https://openalex.org/I78577930']}]}], 'institution_assertions': [], 'countries_distinct_count': 1, 'institutions_distinct_count': 1, 'corresponding_author_ids': [], 'corresponding_institution_ids': [], 'apc_list': None, 'apc_paid': None, 'fwci': 4.755, 'has_fulltext': True, 'fulltext_origin': 'pdf', 'cited_by_count': 193, 'citation_normalized_percentile': {'value': 0.941894, 'is_in_top_1_percent': False, 'is_in_top_10_percent': True}, 'cited_by_percentile_year': {'min': 98, 'max': 99}, 'biblio': {'volume': '107', 'issue': '2', 'first_page': '195', 'last_page': '205'}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T10077', 'display_name': 'Neuroscience and Neuropharmacology Research', 'score': 0.9996, 'subfield': {'id': 'https://openalex.org/subfields/2804', 'display_name': 'Cellular and Molecular Neuroscience'}, 'field': {'id': 'https://openalex.org/fields/28', 'display_name': 'Neuroscience'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, 'topics': [{'id': 'https://openalex.org/T10077', 'display_name': 'Neuroscience and Neuropharmacology Research', 'score': 0.9996, 'subfield': {'id': 'https://openalex.org/subfields/2804', 'display_name': 'Cellular and Molecular Neuroscience'}, 'field': {'id': 'https://openalex.org/fields/28', 'display_name': 'Neuroscience'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T10493', 'display_name': 'Ion channel regulation and function', 'score': 0.9989, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T11818', 'display_name': 'Nicotinic Acetylcholine Receptors Study', 'score': 0.9979, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}], 'keywords': [{'id': 'https://openalex.org/keywords/cys-loop-receptors', 'display_name': 'Cys-loop receptors', 'score': 0.5839721}, {'id': 'https://openalex.org/keywords/gamma-aminobutyric-acid', 'display_name': 'Gamma-Aminobutyric Acid', 'score': 0.5238804}], 'concepts': [{'id': 'https://openalex.org/C50254741', 'wikidata': 'https://www.wikidata.org/wiki/Q62536', 'display_name': 'Ion channel', 'level': 3, 'score': 0.6424364}, {'id': 'https://openalex.org/C104292427', 'wikidata': 'https://www.wikidata.org/wiki/Q899781', 'display_name': 'Protein subunit', 'level': 3, 'score': 0.61569685}, {'id': 'https://openalex.org/C12554922', 'wikidata': 'https://www.wikidata.org/wiki/Q7100', 'display_name': 'Biophysics', 'level': 1, 'score': 0.5948041}, {'id': 'https://openalex.org/C19876734', 'wikidata': 'https://www.wikidata.org/wiki/Q5201170', 'display_name': 'Cys-loop receptors', 'level': 5, 'score': 0.5839721}, {'id': 'https://openalex.org/C185592680', 'wikidata': 'https://www.wikidata.org/wiki/Q2329', 'display_name': 'Chemistry', 'level': 0, 'score': 0.55332565}, {'id': 'https://openalex.org/C2777771985', 'wikidata': 'https://www.wikidata.org/wiki/Q210021', 'display_name': 'gamma-Aminobutyric acid', 'level': 3, 'score': 0.5238804}, {'id': 'https://openalex.org/C2779201268', 'wikidata': 'https://www.wikidata.org/wiki/Q186474', 'display_name': 'Cysteine', 'level': 3, 'score': 0.5181245}, {'id': 'https://openalex.org/C2779535977', 'wikidata': 'https://www.wikidata.org/wiki/Q1342298', 'display_name': 'Xenopus', 'level': 3, 'score': 0.4904003}, {'id': 'https://openalex.org/C170493617', 'wikidata': 'https://www.wikidata.org/wiki/Q208467', 'display_name': 'Receptor', 'level': 2, 'score': 0.48460612}, {'id': 'https://openalex.org/C194544171', 'wikidata': 'https://www.wikidata.org/wiki/Q21105679', 'display_name': 'Gating', 'level': 2, 'score': 0.4632486}, {'id': 'https://openalex.org/C515207424', 'wikidata': 'https://www.wikidata.org/wiki/Q8066', 'display_name': 'Amino acid', 'level': 2, 'score': 0.44465852}, {'id': 'https://openalex.org/C55493867', 'wikidata': 'https://www.wikidata.org/wiki/Q7094', 'display_name': 'Biochemistry', 'level': 1, 'score': 0.42578965}, {'id': 'https://openalex.org/C86803240', 'wikidata': 'https://www.wikidata.org/wiki/Q420', 'display_name': 'Biology', 'level': 0, 'score': 0.27819073}, {'id': 'https://openalex.org/C80161118', 'wikidata': 'https://www.wikidata.org/wiki/Q408520', 'display_name': 'Acetylcholine receptor', 'level': 3, 'score': 0.14184943}, {'id': 'https://openalex.org/C181199279', 'wikidata': 'https://www.wikidata.org/wiki/Q8047', 'display_name': 'Enzyme', 'level': 2, 'score': 0.06346929}, {'id': 'https://openalex.org/C2779570518', 'wikidata': 'https://www.wikidata.org/wiki/Q412256', 'display_name': 'Nicotinic acetylcholine receptor', 'level': 4, 'score': 0.0}, {'id': 'https://openalex.org/C104317684', 'wikidata': 'https://www.wikidata.org/wiki/Q7187', 'display_name': 'Gene', 'level': 2, 'score': 0.0}], 'mesh': [{'descriptor_ui': 'D015640', 'descriptor_name': 'Ion Channel Gating', 'qualifier_ui': 'Q000502', 'qualifier_name': 'physiology', 'is_major_topic': True}, {'descriptor_ui': 'D007473', 'descriptor_name': 'Ion Channels', 'qualifier_ui': 'Q000737', 'qualifier_name': 'chemistry', 'is_major_topic': True}, {'descriptor_ui': 'D011963', 'descriptor_name': 'Receptors, GABA-A', 'qualifier_ui': 'Q000737', 'qualifier_name': 'chemistry', 'is_major_topic': True}, {'descriptor_ui': 'D002731', 'descriptor_name': '4-Chloromercuribenzenesulfonate', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D002731', 'descriptor_name': '4-Chloromercuribenzenesulfonate', 'qualifier_ui': 'Q000494', 'qualifier_name': 'pharmacology', 'is_major_topic': False}, {'descriptor_ui': 'D000818', 'descriptor_name': 'Animals', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D002729', 'descriptor_name': 'Chloromercuribenzoates', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D002729', 'descriptor_name': 'Chloromercuribenzoates', 'qualifier_ui': 'Q000494', 'qualifier_name': 'pharmacology', 'is_major_topic': False}, {'descriptor_ui': 'D005020', 'descriptor_name': 'Ethyl Methanesulfonate', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D005020', 'descriptor_name': 'Ethyl Methanesulfonate', 'qualifier_ui': 'Q000031', 'qualifier_name': 'analogs & derivatives', 'is_major_topic': False}, {'descriptor_ui': 'D005020', 'descriptor_name': 'Ethyl Methanesulfonate', 'qualifier_ui': 'Q000494', 'qualifier_name': 'pharmacology', 'is_major_topic': False}, {'descriptor_ui': 'D005260', 'descriptor_name': 'Female', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D007202', 'descriptor_name': 'Indicators and Reagents', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D007202', 'descriptor_name': 'Indicators and Reagents', 'qualifier_ui': 'Q000494', 'qualifier_name': 'pharmacology', 'is_major_topic': False}, {'descriptor_ui': 'D007461', 'descriptor_name': 'Iodoacetates', 'qualifier_ui': 'Q000494', 'qualifier_name': 'pharmacology', 'is_major_topic': False}, {'descriptor_ui': 'D007461', 'descriptor_name': 'Iodoacetates', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D019807', 'descriptor_name': 'Iodoacetic Acid', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D015640', 'descriptor_name': 'Ion Channel Gating', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D007473', 'descriptor_name': 'Ion Channels', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D007473', 'descriptor_name': 'Ion Channels', 'qualifier_ui': 'Q000187', 'qualifier_name': 'drug effects', 'is_major_topic': False}, {'descriptor_ui': 'D007473', 'descriptor_name': 'Ion Channels', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': False}, {'descriptor_ui': 'D016297', 'descriptor_name': 'Mutagenesis, Site-Directed', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D016297', 'descriptor_name': 'Mutagenesis, Site-Directed', 'qualifier_ui': 'Q000502', 'qualifier_name': 'physiology', 'is_major_topic': False}, {'descriptor_ui': 'D009153', 'descriptor_name': 'Mutagens', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D009153', 'descriptor_name': 'Mutagens', 'qualifier_ui': 'Q000494', 'qualifier_name': 'pharmacology', 'is_major_topic': False}, {'descriptor_ui': 'D009865', 'descriptor_name': 'Oocytes', 'qualifier_ui': 'Q000502', 'qualifier_name': 'physiology', 'is_major_topic': False}, {'descriptor_ui': 'D009865', 'descriptor_name': 'Oocytes', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D018408', 'descriptor_name': 'Patch-Clamp Techniques', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D051381', 'descriptor_name': 'Rats', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D011963', 'descriptor_name': 'Receptors, GABA-A', 'qualifier_ui': 'Q000187', 'qualifier_name': 'drug effects', 'is_major_topic': False}, {'descriptor_ui': 'D011963', 'descriptor_name': 'Receptors, GABA-A', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': False}, {'descriptor_ui': 'D011963', 'descriptor_name': 'Receptors, GABA-A', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D013439', 'descriptor_name': 'Sulfhydryl Reagents', 'qualifier_ui': 'Q000494', 'qualifier_name': 'pharmacology', 'is_major_topic': False}, {'descriptor_ui': 'D013439', 'descriptor_name': 'Sulfhydryl Reagents', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D014981', 'descriptor_name': 'Xenopus', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D020245', 'descriptor_name': 'p-Chloromercuribenzoic Acid', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}], 'locations_count': 4, 'locations': [{'is_oa': True, 'landing_page_url': 'https://doi.org/10.1085/jgp.107.2.195', 'pdf_url': 'https://rupress.org/jgp/article-pdf/107/2/195/1143778/195.pdf', 'source': {'id': 'https://openalex.org/S168548653', 'display_name': 'The Journal of General Physiology', 'issn_l': '0022-1295', 'issn': ['0022-1295', '1540-7748'], 'is_oa': True, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310315676', 'host_organization_name': 'Rockefeller University Press', 'host_organization_lineage': ['https://openalex.org/P4310315676'], 'host_organization_lineage_names': ['Rockefeller University Press'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, {'is_oa': True, 'landing_page_url': 'https://europepmc.org/articles/pmc2219269', 'pdf_url': 'https://europepmc.org/articles/pmc2219269?pdf=render', 'source': {'id': 'https://openalex.org/S4306400806', 'display_name': 'Europe PMC (PubMed Central)', 'issn_l': None, 'issn': None, 'is_oa': True, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1303153112', 'host_organization_name': 'European Bioinformatics Institute', 'host_organization_lineage': ['https://openalex.org/I1303153112'], 'host_organization_lineage_names': ['European Bioinformatics Institute'], 'type': 'repository'}, 'license': 'cc-by-nc-sa', 'license_id': 'https://openalex.org/licenses/cc-by-nc-sa', 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, {'is_oa': True, 'landing_page_url': 'https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2219269', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S2764455111', 'display_name': 'PubMed Central', 'issn_l': None, 'issn': None, 'is_oa': True, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, {'is_oa': False, 'landing_page_url': 'https://pubmed.ncbi.nlm.nih.gov/8833341', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4306525036', 'display_name': 'PubMed', 'issn_l': None, 'issn': None, 'is_oa': False, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}], 'best_oa_location': {'is_oa': True, 'landing_page_url': 'https://doi.org/10.1085/jgp.107.2.195', 'pdf_url': 'https://rupress.org/jgp/article-pdf/107/2/195/1143778/195.pdf', 'source': {'id': 'https://openalex.org/S168548653', 'display_name': 'The Journal of General Physiology', 'issn_l': '0022-1295', 'issn': ['0022-1295', '1540-7748'], 'is_oa': True, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310315676', 'host_organization_name': 'Rockefeller University Press', 'host_organization_lineage': ['https://openalex.org/P4310315676'], 'host_organization_lineage_names': ['Rockefeller University Press'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, 'sustainable_development_goals': [], 'grants': [], 'datasets': [], 'versions': [], 'referenced_works_count': 49, 'referenced_works': ['https://openalex.org/W1479710013', 'https://openalex.org/W1560588976', 'https://openalex.org/W1570185216', 'https://openalex.org/W1574744729', 'https://openalex.org/W1597869519', 'https://openalex.org/W1915376905', 'https://openalex.org/W1978854127', 'https://openalex.org/W1986315354', 'https://openalex.org/W1991039749', 'https://openalex.org/W1994522080', 'https://openalex.org/W1997631508', 'https://openalex.org/W2004491592', 'https://openalex.org/W2014853483', 'https://openalex.org/W2021656295', 'https://openalex.org/W2023468172', 'https://openalex.org/W2024011126', 'https://openalex.org/W2025440222', 'https://openalex.org/W2025810192', 'https://openalex.org/W2029610734', 'https://openalex.org/W2031226921', 'https://openalex.org/W2031368977', 'https://openalex.org/W2036513882', 'https://openalex.org/W2037992892', 'https://openalex.org/W2038591883', 'https://openalex.org/W2048046166', 'https://openalex.org/W2051308032', 'https://openalex.org/W2053718517', 'https://openalex.org/W2057165567', 'https://openalex.org/W2057264061', 'https://openalex.org/W2058896100', 'https://openalex.org/W2064487331', 'https://openalex.org/W2073286007', 'https://openalex.org/W2078615673', 'https://openalex.org/W2079977484', 'https://openalex.org/W2081025398', 'https://openalex.org/W2088814430', 'https://openalex.org/W2125828036', 'https://openalex.org/W2127420531', 'https://openalex.org/W2129488948', 'https://openalex.org/W2138937351', 'https://openalex.org/W2139584976', 'https://openalex.org/W2176076159', 'https://openalex.org/W2407659316', 'https://openalex.org/W2417577490', 'https://openalex.org/W3001179380', 'https://openalex.org/W3088697837', 'https://openalex.org/W4237680261', 'https://openalex.org/W4249054529', 'https://openalex.org/W4295682975'], 'related_works': ['https://openalex.org/W2443450049', 'https://openalex.org/W2427495887', 'https://openalex.org/W2410643920', 'https://openalex.org/W2232251872', 'https://openalex.org/W2124669621', 'https://openalex.org/W2047273628', 'https://openalex.org/W1975539866', 'https://openalex.org/W1966343975', 'https://openalex.org/W1855660343', 'https://openalex.org/W1518135840'], 'abstract_inverted_index': {'The': [0, 19, 246, 274], 'gamma-aminobutyric': [1, 22], 'acid': [2, 23, 73], 'type': [3], 'A': [4], '(GABA(A))': [5], 'receptors': [6, 13, 28], 'are': [7, 198], 'the': [8, 15, 26, 30, 49, 52, 56, 71, 77, 97, 102, 110, 125, 128, 151, 170, 177, 201, 208, 219, 230, 241, 256, 260, 264, 277, 285, 292], 'major': [9], 'inhibitory,': [10], 'postsynaptic,': [11], 'neurotransmitter': [12], 'in': [14, 94, 120, 145, 200, 221, 240, 255], 'central': [16], 'nervous': [17], 'system.': [18], 'binding': [20], 'of': [21, 32, 41, 51, 101, 127, 176, 218, 224, 243, 248, 259, 276, 287], '(GABA)': [24], 'to': [25, 69, 106, 131, 156, 283], 'GABA(A)': [27, 53], 'induces': [29], 'opening': [31], 'an': [33, 222, 234], 'anion-selective': [34], 'channel': [35, 152, 261], 'that': [36, 75, 142, 162, 174, 229, 237, 263, 291], 'remains': [37], 'open': [38], 'for': [39], 'tens': [40], 'milliseconds': [42], 'before': [43], 'it': [44], 'closes.': [45], 'To': [46, 81], 'understand': [47], 'how': [48], 'structure': [50], 'receptor': [54], 'determines': [55], 'functional': [57], 'properties': [58], 'such': [59, 163], 'as': [60, 250, 252, 269, 271, 298, 300], 'ion': [61, 63, 78], 'conduction,': [62], 'selectivity': [64], 'and': [65, 95, 117, 161, 195], 'gating': [66], 'we': [67, 84], 'sought': [68], 'identify': [70], 'amino': [72], 'residues': [74, 88, 144, 249], 'line': [76], 'conducting': [79], 'channel.': [80, 171, 202], 'accomplish': [82], 'this': [83, 301], 'mutated': [85], '26': [86], 'consecutive': [87], '(250-275),': [89], 'one': [90, 216], 'at': [91, 267, 296], 'a': [92, 204], 'time,': [93], 'flanking': [96], 'M2': [98, 231], 'membrane-spanning': [99, 146], 'segment': [100, 232], 'rat': [103], 'alpha1': [104, 112, 179, 181, 183, 185, 187, 189, 191, 193, 196, 212, 244, 253, 272, 288], 'subunit': [105, 113], 'cysteine.': [107], 'We': [108, 123, 140, 172, 227], 'expressed': [109], 'mutant': [111], 'with': [114], 'wild-type': [115], 'beta1': [116], 'gamma2': [118], 'subunits': [119], 'Xenopus': [121], 'oocytes.': [122], 'probed': [124], 'accessibility': [126], 'engineered': [129], 'cysteine': [130], 'covalent': [132], 'modification': [133, 157, 164, 247], 'by': [134, 158], 'charged,': [135], 'sulfhydryl-specific': [136], 'reagents': [137, 160], 'added': [138], 'extracellularly.': [139], 'assume': [141], 'among': [143], 'segments,': [147], 'only': [148], 'those': [149], 'lining': [150], 'would': [153, 165], 'be': [154], 'susceptible': [155], 'polar': [159], 'irreversibly': [166], 'alter': [167], 'conduction': [168], 'through': [169], 'infer': [173, 228], 'nine': [175], 'residues,': [178, 210], 'Val257,': [180], 'Thr26l,': [182], 'Thr262,': [184, 213], 'Leu264,': [186], 'Thr265,': [188], 'Thr268,': [190], 'Ile27l,': [192], 'Ser272': [194], 'Asn275': [197], 'exposed': [199, 209], 'On': [203], 'helical': [205], 'wheel': [206], 'plot,': [207], 'except': [211], 'lie': [214], 'on': [215], 'side': [217], 'helix': [220, 236], 'arc': [223], '120': [225], 'degrees.': [226], 'forms': [233], 'alpha': [235], 'is': [238, 266, 295], 'interrupted': [239], 'region': [242], 'Thr262.': [245], 'cytoplasmic': [251, 270, 299], 'Val257': [254], 'closed': [257], 'state': [258], 'suggests': [262, 290], 'gate': [265], 'least': [268, 297], 'Val257.': [273], 'ability': [275], 'positively': [278], 'charged': [279], 'reagent': [280], 'methanethiosulfonate': [281], 'ethylammonium': [282], 'reach': [284], 'level': [286], 'Thr261': [289], 'charge-selectivity': [293], 'filter': [294], 'residue.': [302]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2163119877', 'counts_by_year': [{'year': 2022, 'cited_by_count': 2}, {'year': 2021, 'cited_by_count': 1}, {'year': 2020, 'cited_by_count': 1}, {'year': 2019, 'cited_by_count': 1}, {'year': 2018, 'cited_by_count': 2}, {'year': 2017, 'cited_by_count': 1}, {'year': 2016, 'cited_by_count': 4}, {'year': 2015, 'cited_by_count': 4}, {'year': 2014, 'cited_by_count': 4}, {'year': 2013, 'cited_by_count': 2}, {'year': 2012, 'cited_by_count': 3}], 'updated_date': '2024-12-09T01:01:28.659416', 'created_date': '2016-06-24'}