Title: Biochemical and functional effects of nucleoside transport inhibition in the isolated cat heart
Abstract: With increasing periods of normothermic ischemia, increasing amounts of mainly inosine and hypoxanthine are released in reperfusates of isolated working cat hearts. Nucleoside transport inhibition (soluflazine; 1 x 10(-7) M) markedly reduces the total release, but increases the release of adenosine. Tissue levels of adenine nucleotides are reduced by 50% after 32 min of ischemia with an almost equivalent, parallel, rise in inosine and hypoxanthine. Subsequent reperfusion leads to a complete removal of the catabolites and a restoration of the energy charge, but not to any recovery of the sum of nucleotides. Nucleoside transport inhibition has no effect on the changes in the nucleotides, but induces a marked accumulation of adenosine during ischemia and prevents the rapid escape of the nucleosides--not of hypoxanthine--upon reperfusion. Cardiac function markedly deteriorates after 20 and 32 min of ischemia. Transport inhibition completely prevents the decrease in cardiac output and pressure-rate product without any effect on normoxic performance. It is suggested that the prolonged presence of adenosine and of inosine may exert a protective effect.
Publication Year: 1989
Publication Date: 1989-08-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
Access and Citation
Cited By Count: 34
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