The Prodrug Activator EtaA from Mycobacterium tuberculosis Is a Baeyer-Villiger Monooxygenase

Name: Work Video:
Duration: 3 min 30 s
Marco W. Fraaije; Nanne M. Kamerbeek; Annelies J. Heidekamp; R. Fortin; Dick B. Janssen
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463, 561, 605, 782, 808, 1111, 1204, 1244, 1318, 1374, 1452, 1511, 2746, 2788, 2807, 2837, 2857, 2922, 3010, 3051, 3152, 3203, 3231, 3240, 3252, 3277, 3309, 3453, 3546, 3645, 3775, 3804, 3837, 3921, 3933, 4012, 4160, 4217, 4307, 4318], 'responsible': [9, 220, 607], 'for': [10, 221, 518, 551, 608, 859, 1056, 1378, 1384, 1952, 2321, 2507, 2907, 2984, 2999, 3067, 3287, 3449, 3460, 3499, 3588, 3711, 4148, 4210, 4268], 'activation': [11, 222, 793, 1166, 3551], 'of': [12, 39, 58, 72, 94, 117, 120, 122, 133, 155, 161, 175, 186, 223, 250, 269, 283, 305, 328, 331, 333, 344, 366, 372, 386, 397, 428, 439, 458, 468, 474, 529, 574, 576, 612, 693, 699, 727, 732, 786, 1002, 1134, 1140, 1255, 1286, 1321, 1339, 1363, 1365, 1402, 1444, 1567, 1606, 1611, 1674, 1699, 1786, 1798, 1810, 1872, 1934, 1939, 1976, 1982, 2135, 2194, 2208, 2255, 2266, 2278, 2286, 2334, 2346, 2360, 2367, 2393, 2460, 2470, 2496, 2521, 2620, 2654, 2676, 2682, 2686, 2706, 2717, 2724, 2741, 2752, 2803, 2864, 2895, 2909, 2954, 3001, 3021, 3032, 3038, 3059, 3076, 3091, 3129, 3141, 3176, 3181, 3196, 3279, 3316, 3377, 3387, 3390, 3400, 3429, 3447, 3470, 3476, 3485, 3492, 3573, 3652, 3663, 3693, 3704, 3771, 3810, 3822, 3846, 3855, 3879, 3890, 3919, 3955, 3974, 3981, 3989, 4017, 4051, 4060, 4062, 4069, 4077, 4084, 4103, 4115, 4123, 4136, 4172, 4181, 4194, 4212, 4220, 4238, 4271, 4281, 4286, 4290, 4300, 4330], 'several': [13, 224, 536, 1438, 3934, 4026], 'thioamide': [14, 225, 537, 577, 1386, 1396, 3379], 'prodrugs': [15, 226, 1070], 'in': [16, 45, 167, 227, 256, 378, 501, 703, 707, 724, 834, 842, 890, 1017, 1137, 1160, 1282, 1504, 1532, 1732, 1795, 1820, 1841, 1857, 1893, 2052, 2390, 2412, 2421, 2590, 2607, 2673, 2700, 2732, 2784, 2853, 2977, 3028, 3103, 3157, 3186, 3272, 3558, 3567, 3638, 3842, 3881, 3896, 4066, 4142, 4201, 4313, 4322], 'Mycobacterium': [17, 228, 601, 704, 838], 'tuberculosis.': [18, 210, 229, 421], 'It': [19, 62, 230, 273, 1240, 3200, 3642, 3801, 3930], 'was': [20, 63, 231, 274, 603, 615, 665, 695, 1168, 1645, 1679, 1730, 1771, 1813, 1876, 1924, 1945, 1963, 1990, 2009, 2022, 2042, 2079, 2102, 2130, 2174, 2259, 2280, 2326, 2362, 2418, 2429, 2465, 2473, 2515, 2532, 2545, 2622, 2668, 2835, 2914, 2988, 2993, 3096, 3134, 3190, 3201, 3206, 3424, 3562, 3748, 3802, 3931, 4010, 4019, 4263, 4296, 4305], 'found': [21, 232, 666, 1170, 1373, 2836, 2994, 3202, 3563, 3587, 3803, 3932, 4011, 4166, 4209, 4306], 'purified': [23, 234, 1619, 1877, 2043, 2669, 2693, 2877, 2915, 2956, 2986, 3053], 'displays': [25, 236, 810], 'remarkably': [27, 238, 3457], 'low': [28, 239, 2734, 3324, 3467, 3483], 'activity': [29, 179, 240, 390, 1326, 1520, 2252, 2888, 3327], 'with': [30, 129, 241, 340, 443, 686, 719, 844, 1437, 1518, 1776, 1851, 1861, 1931, 1947, 1972, 1995, 2031, 2121, 2187, 2221, 2253, 2426, 2566, 2738, 2800, 2874, 3143, 3193, 3220, 3222, 3228, 3249, 3274, 3306, 3332, 3649, 3850, 3948, 4278], 'the': [31, 37, 46, 66, 75, 92, 123, 156, 168, 173, 242, 248, 257, 277, 286, 303, 334, 367, 379, 384, 424, 502, 527, 544, 572, 582, 609, 671, 677, 700, 714, 783, 845, 891, 998, 1053, 1065, 1069, 1138, 1141, 1151, 1205, 1245, 1271, 1283, 1319, 1337, 1366, 1375, 1400, 1431, 1442, 1505, 1512, 1562, 1568, 1607, 1621, 1634, 1702, 1734, 1742, 1769, 1783, 1787, 1796, 1823, 1879, 1921, 1942, 1948, 1960, 1970, 1988, 1993, 2003, 2005, 2020, 2073, 2077, 2095, 2098, 2200, 2224, 2256, 2264, 2284, 2287, 2319, 2332, 2347, 2365, 2391, 2458, 2471, 2579, 2665, 2674, 2714, 2718, 2725, 2728, 2739, 2747, 2785, 2858, 2886, 2910, 2955, 2985, 2997, 3002, 3017, 3022, 3033, 3039, 3043, 3052, 3060, 3073, 3153, 3158, 3182, 3188, 3204, 3210, 3214, 3223, 3232, 3241, 3253, 3275, 3280, 3310, 3378, 3384, 3398, 3401, 3427, 3439, 3461, 3554, 3650, 3661, 3676, 3687, 3700, 3705, 3753, 3768, 3815, 3825, 3831, 3838, 3843, 3851, 3856, 3888, 3897, 3959, 3972, 3979, 4045, 4049, 4052, 4063, 4067, 4070, 4075, 4085, 4100, 4108, 4116, 4124, 4127, 4137, 4143, 4151, 4163, 4179, 4192, 4213, 4218, 4230, 4235, 4239, 4258, 4266, 4269, 4276, 4283, 4301, 4308, 4314, 4323, 4328, 4332, 4340], 'antitubercular': [32, 243, 531, 553, 610, 784, 1060, 1201, 1380], 'prodrug': [33, 206, 244, 417, 701, 715, 814, 1571, 3462, 3550], 'ethionamide.': [34, 245, 4149], 'Hinted': [35, 246], 'by': [36, 183, 188, 247, 394, 399, 584, 670, 684, 697, 794, 801, 1077, 1086, 1174, 1275, 1336, 1688, 1748, 1815, 1889, 1965, 2024, 2144, 2163, 2262, 2282, 2364, 2424, 2431, 2517, 2541, 2599, 2624, 2754, 2845, 3024, 3099, 3136, 3209, 3226, 3396, 3617, 3758, 3779, 3848, 3887, 3928, 3945, 3967, 4021, 4113, 4224], 'presence': [38, 249, 1443, 2392, 2675, 3889, 4270], 'Baeyer-Villiger': [41, 82, 89, 252, 293, 300, 1457, 3885, 3991, 4042, 4104], 'sequence': [43, 254, 1427, 1433, 1448, 1507, 1516, 2787, 2797, 3590, 3894, 3899], 'motif': [44, 255, 3895], 'sequence,': [48, 259], 'we': [49, 260, 3766, 3915], 'have': [50, 261, 539, 1738, 3922], 'been': [51, 262, 540, 857, 888, 1006, 1109, 1242, 1316, 1372, 1528, 1739, 1746, 2758, 2905, 2933, 3330, 3615, 3923], 'able': [52, 112, 263, 323, 3244], 'to': [53, 74, 113, 264, 285, 324, 470, 565, 667, 740, 792, 822, 831, 849, 1008, 1157, 1171, 1258, 1560, 1926, 2011, 2148, 2587, 2604, 2703, 2713, 2811, 2840, 2871, 2995, 3112, 3245, 3490, 3506, 3544, 3564, 3585, 3698, 3750, 3824, 3925, 4001, 4032, 4155, 4343], 'identify': [54, 265], 'large': [56, 267, 2866], 'number': [57, 268, 457, 2802], 'novel': [59, 270], 'substrates.': [61, 272], 'discovered': [64, 275], 'enzyme': [67, 278, 828, 1989, 2007, 2021, 2086, 2100, 2129, 2464, 2694, 2748, 2859, 2897, 2958, 3023, 3054, 3154, 3189, 3205, 3233, 3242, 3254, 3311, 3561, 4240, 4277, 4309], 'converts': [68, 279], 'wide': [70, 281], 'range': [71, 282, 3503, 3918, 4016, 4145], 'ketones': [73, 96, 101, 284, 307, 312, 2258, 3920, 3950, 4018, 4036, 4064], 'corresponding': [76, 287, 672, 1142, 2702, 3612, 4046], 'esters': [77, 288], 'or': [78, 289, 472, 1341, 1853, 2188, 2814, 3962], 'lactones': [79, 290], 'via': [80, 291, 4040], 'reaction,': [83, 294], 'indicating': [84, 295, 1203, 2921, 3009, 4317], 'represents': [87, 298], 'monooxygenase.': [90, 301, 1523], 'With': [91, 302, 4048], 'exception': [93, 304, 3651, 4050], 'aromatic': [95, 306], '(phenylacetone': [97, 308], 'and': [98, 104, 195, 309, 315, 406, 452, 507, 556, 748, 1072, 1391, 1394, 1530, 1557, 1565, 1582, 1596, 1600, 1624, 1633, 1672, 1693, 1701, 1713, 1778, 1780, 1807, 1832, 1855, 1865, 1891, 1919, 1950, 1978, 2036, 2070, 2146, 2161, 2165, 2202, 2247, 2313, 2339, 2378, 2408, 2437, 2463, 2501, 2534, 2538, 2557, 2577, 2610, 2651, 2660, 2884, 2970, 2981, 3046, 3124, 3147, 3247, 3257, 3369, 3473, 3611, 3940, 4057, 4080, 4294], 'benzylacetone),': [99, 310], 'long-chain': [100, 311, 4027, 4053], '(e.g.': [102, 313], '2-hexanone': [103, 314, 4031], '2-dodecanone)': [105, 316], 'also': [106, 111, 317, 322, 563, 789, 820, 1052, 1169, 3329, 3655, 4037, 4092], 'are': [107, 318, 461, 559, 562, 1074, 2808, 3284, 3709, 3760, 4038, 4141], 'converted.': [108, 319], 'catalyze': [114, 325], 'enantioselective': [115, 326, 2618, 3808], 'sulfoxidation': [116, 327, 2619, 3376, 3755], 'methyl-p-tolylsulfide.': [118, 329], 'Conversion': [119, 330, 2277, 3389], 'all': [121, 332, 2254, 2809, 2908, 3000, 3180, 3278, 3694, 3703, 4061, 4135, 4211, 4299], 'substrates': [125, 336, 3610, 3937, 4215], 'relatively': [127, 338, 2865, 3374, 3466], 'slow': [128, 339, 3314, 3375, 4227], 'typical': [130, 341, 2961, 3935], 'kcat': [131, 149, 342, 360, 3468, 4139, 4207], 'values': [132, 343, 4140, 4153, 4208], 'around': [134, 345, 2968], '0.02': [135, 346], 's–1.': [136, 347], 'The': [137, 348, 515, 690, 2047, 2085, 2128, 2513, 2528, 2617, 2692, 2795, 2975, 3063, 3238, 3442, 3560, 3820, 4186, 4205], 'best': [138, 349, 4164], 'substrate': [139, 196, 350, 407, 1563, 3459, 3784, 4165], 'so': [141, 352, 4167], 'far': [142, 353], 'phenylacetone': [144, 162, 355, 373, 2325, 2338, 3150, 4161], '(Km': [145, 356], '=': [146, 150, 357, 361, 2273, 2295, 2402, 3082, 3118], '61': [147, 358], 'μm,': [148, 359, 2118], '0.017': [151, 362], 's–1).': [152, 363], 'Redox': [153, 364], 'monitoring': [154, 365, 2263, 2283], 'flavin': [157, 368, 2479, 2928, 2962, 3011, 3034, 3061, 3262, 3857], 'cofactor': [158, 369, 3035, 3045, 3858, 4183, 4196], 'during': [159, 370, 543, 3161, 4184, 4197, 4241, 4288], 'turnover': [160, 371, 3163, 4175, 4188, 4256], 'indicates': [163, 374, 807, 3251, 4178, 4191], 'step': [166, 377, 1167, 4321], 'reductive': [169, 380, 4324, 4333], 'half-reaction': [170, 381, 4248, 4325], 'limiting': [172, 383, 3426, 4327], 'rate': [174, 385, 2207, 3428, 3484, 3980, 4219, 4329], 'catalysis.': [176, 387], 'Intriguingly,': [177, 388], 'could': [180, 391, 716, 2087, 2481, 2689, 2781, 2789, 3069, 3264, 3380, 3392, 3958, 3995, 4091], 'be': [181, 392, 465, 668, 717, 790, 832, 1009, 1158, 1172, 1334, 2088, 2482, 2690, 2782, 2812, 2996, 3070, 3265, 3381, 3393, 3491, 3565, 3926, 3965, 3996, 4093, 4245], 'increased': [182, 393], 'one': [184, 395, 471, 803], 'order': [185, 396], 'magnitude': [187, 398], 'adding': [189, 400, 2432, 2518, 3137], 'bovine': [190, 401, 2234], 'serum': [191, 402, 1467, 1479], 'albumin.': [192, 403], 'This': [193, 404, 806, 840, 1145, 1164, 1264, 1357, 1524, 2709, 2991, 3270, 3581, 3998], 'reactivity': [194, 405, 1566], 'acceptance-profiling': [197, 408], 'study': [198, 409, 1561, 2855], 'provides': [199, 410], 'valuable': [200, 411], 'information': [201, 412], 'concerning': [202, 413], 'this': [203, 414, 534, 835, 1197, 2854, 2901, 3764, 3811, 4250], 'activator': [207, 418, 702, 1572, 3463], 'from': [208, 419, 430, 600, 799, 1328, 1389, 1586, 1593, 1603, 1627, 1639, 1773, 1883, 1992, 2223, 2229, 2585, 2602, 2670, 2727, 2869, 3504, 3680, 4030, 4339], 'M.': [209, 420, 623, 734, 758, 850, 860, 892, 966, 1032, 1222, 3720, 3724], 'Tuberculosis': [422], "world's": [425], 'leading': [426], 'cause': [427], 'death': [429], 'single': [432, 2698], 'infectious': [433], 'organism.': [434], 'Standard': [435], 'tuberculosis': [436, 459, 602, 735], 'chemotherapy': [437], 'consists': [438], 'lengthy': [441], 'treatment': [442], 'at': [444, 1869, 1955, 2038, 2168, 2204, 2268, 2290, 2352, 2475, 2484, 2510, 2595, 2614, 2664, 2967, 2979, 3116, 3404, 4260], 'least': [445], 'two': [446, 741, 1078, 1251, 1704], 'so-called': [447], 'first-line': [448], 'drugs,': [449, 746], 'e.g.': [450], 'isoniazid': [451, 1073, 1083, 1269], 'rifampicin.': [453], 'Unfortunately,': [454], 'an': [455, 811, 883, 1011, 1212, 2026, 2567, 3194, 3548, 3807], 'increasing': [456], 'cases': [460], 'encountered': [462, 560], 'cannot': [464, 1333], 'treated': [466], 'because': [467, 796, 817, 3432, 3495], 'resistance': [469, 588, 712], 'more': [473, 555, 557, 4003], 'these': [475, 590, 3949, 4254], 'drugs': [476, 538, 591], '(1Dye': [477], 'C.': [478, 654, 912, 944, 1024, 1689, 3362], 'Williams': [479], 'B.G.': [480, 982], 'Espinal': [481], 'M.A.': [482, 954, 956], 'Raviglione': [483], 'M.C.': [484], 'Science.': [485, 1308, 1589], '2002;': [486, 1046, 1128, 1192, 1496, 2772, 2829, 2948, 3300, 3418, 3632, 3735, 3911], '295:': [487], '2042-2046Google': [488], 'Scholar,': [489, 640, 987, 3356, 3524], '2WHO/IUATLD': [490], 'Global': [491], 'Project': [492], 'on': [493, 526, 1324, 2199, 2547, 3016, 3686], 'Anti-Tuberculosis': [494], 'Drug': [495, 499], 'Resistance': [496, 500], 'Surveillance': [497], 'Antituberculosis': [498], 'World.': [503], 'Report': [504], '2:': [505], 'Prevalence': [506], 'Trends.': [508], 'World': [509], 'Health': [510], 'Organization,': [511], 'Geneva,': [512], 'Switzerland2002Google': [513], 'Scholar).': [514, 663, 775, 871, 1049, 1131, 1195, 1239, 1312, 1356, 1499, 1766, 2386, 2775, 2832, 2951, 3303, 3421, 3538, 3635, 3738, 3800], 'last': [516, 545], 'resort': [517], 'combating': [519], 'such': [520, 3547, 3669], '(multi)-drug-resistant': [521], 'mycobacterial': [522], 'infections': [523, 558], 'relies': [524], 'mainly': [525, 3156, 3814, 4311], 'action': [528, 575, 1364], 'second-line': [530], 'drugs.': [532], 'For': [533, 1721, 1843, 2317, 2330, 3674, 3763, 4121, 4253, 4298], 'reason,': [535, 3765], 'successfully': [541], 'employed': [542], 'decades': [546], '(Fig.': [547, 2973, 3198], '1).': [548], 'However,': [549, 1196, 2776, 3970], 'as': [550, 579, 581, 1178, 1211, 1331, 1647, 1684, 2328, 2696, 2791, 2843, 2861, 2916, 2935, 3371, 3578, 3783, 4146, 4265], 'other': [552, 742, 1379, 1608, 2804, 3333, 3589, 3706, 3780], 'agents,': [554], 'resistant': [564], 'thioamide-based': [566], 'treatment.': [567], 'Despite': [568], 'their': [569], 'widespread': [570], 'use,': [571], 'mode': [573, 1362], 'antibiotics': [578], 'well': [580, 1058], 'mechanism': [583], 'which': [585, 614, 676, 1733, 2930, 4247], 'mycobacteria': [586], 'acquire': [587], 'against': [589], 'has': [592, 737, 856, 887, 1005, 1107, 1156, 1199, 1241, 1315, 1371, 1526, 1745, 2757, 2904, 2931, 3328, 3614], 'remained': [593], 'obscure.A': [594], 'few': [595], 'years': [596], 'ago': [597], 'gene': [599, 721, 885, 1678, 1770], 'effect': [611, 683, 785, 1320, 2333, 3556], 'ethionamide,': [613, 4291], 'named': [616], 'etaA': [617, 720, 884, 1677, 1683], '(3DeBarber': [618, 753, 1217], 'A.E.': [619, 754, 1218], 'Mdluli': [620, 755, 1219], 'K.': [621, 756, 898, 916, 928, 942, 952, 962, 974, 976, 1220, 1344], 'Bosman': [622, 757, 1221], 'Bekker': [624, 759, 1223], 'L.G.': [625, 760, 1224], 'Barry': [626, 761, 1225], 'C.E.': [627, 762, 1226], 'II': [628, 763, 1227, 2554], 'I': [629, 764, 1228], 'Proc.': [630, 765, 1229], 'Natl.': [631, 766, 1230], 'Acad.': [632, 767, 1231], 'Sci.': [633, 768, 1232], 'U.': [634, 769, 1233], 'S.': [635, 648, 770, 930, 938, 948, 960, 964, 972, 978, 1100, 1234, 2374], 'A.': [636, 771, 934, 1120, 1184, 1235, 1289, 1293, 1694, 2764, 2940, 3410, 3624, 3716, 3718], '2000;': [637, 660, 772, 1236], '97:': [638, 773, 1237], '9677-9682Google': [639, 774, 1238], '4Baulard': [641], 'A.R.': [642, 1409], 'Betts': [643], 'J.C.': [644, 1040], 'Engohang-Ndong': [645], 'J.': [646, 657, 900, 946, 968, 1125, 1189, 1349, 1551, 1666, 2769, 2945, 3351, 3415, 3629, 3732], 'Quan': [647], 'McAdam': [649], 'R.A.': [650], 'Brennan': [651, 1418], 'P.J.': [652, 1419], 'Locht': [653], 'Besra': [655, 1027, 1416], 'G.S.': [656, 1028, 1417], 'Biol.': [658, 1126, 1190, 2770, 2946, 3416, 3630, 3733], 'Chem.': [659, 1127, 1191, 1351, 2771, 2947, 3417, 3631, 3734], '275:': [661], '28326-28331Google': [662], 'Ethionamide': [664], 'activated': [669, 678, 1076, 1085, 1159, 1268, 1322, 1367], 'protein': [673, 1014, 1506, 2688, 2720, 2920, 2987, 3047], 'EtaA,': [674, 2876], 'after': [675, 1250, 3167, 3217], 'product': [679, 1147, 1180, 1215, 1248, 2289, 2488, 3403], 'exerts': [680], 'lethal': [682], 'interacting': [685], 'final': [688, 1000, 1054, 1067, 1179, 1272, 1376], 'target.': [689], 'activating': [691, 827, 3385], 'role': [692, 3386, 3878], 'confirmed': [696], 'overexpression': [698], 'smegmatis,': [705], 'resulting': [706, 723, 1840, 1922], 'ethionamide-hypersensitive': [708], 'mycobacteria.': [709, 3559], 'Clinically': [710], 'observed': [711, 858, 1317, 2759, 3005, 3088, 3331, 3557, 3754, 3832], 'toward': [713, 2889], 'associated': [718], 'mutations': [722], 'functional': [725], 'impairment': [726], 'EtaA.': [728, 3092, 3388, 3946], 'A': [729, 3127, 3323], 'thorough': [730], 'analysis': [731, 873, 2544, 2953], 'ethionamide-resistant': [733], 'isolates': [736], 'revealed': [738, 1434, 1510, 3308], 'cross-resistance': [739, 852], 'widely': [743], 'used': [744, 821, 1462, 1474, 1731, 2327, 2906, 3710], 'thiocarbamide-containing': [745], 'thiacetazone': [747], 'thiocarlide': [749, 1393], '(see': [750, 2891], 'Fig.': [751], '1)': [752], 'In': [776, 1500, 2072, 2094, 3539, 3659, 4074], 'addition,': [777], 'it': [778, 797, 1106, 1314, 1370, 2834, 2923, 3541, 3747, 4009, 4243, 4304], 'very': [780, 2733, 2899], 'likely': [781], 'prothionamide': [787], 'can': [788, 3552, 3648, 3667, 4244], 'linked': [791], 'differs': [798, 1327], 'ethionamide': [800, 818, 1004, 1071, 1135, 1256, 1323, 1390, 2279, 2890, 3368, 3391, 3430, 3450, 3454, 3577], 'only': [802, 3689], 'methyl': [804, 2396], 'group.': [805], 'exceptional': [812], 'broad': [813, 4015], 'acceptance.': [815], 'Furthermore,': [816, 1369, 1441], 'treat': [823], 'leprosy,': [824], 'similar': [826, 848, 2881, 3584, 4206], 'expected': [830, 3960, 4071], 'present': [833, 4312], 'related': [836, 1395], 'pathogenic': [837], 'leprae.': [839], 'line': [843, 3273], 'observation': [846, 3276], 'that,': [847, 876], 'tuberculosis,': [851], 'among': [853], 'above-mentioned': [854], 'thioamides': [855, 1381], 'leprae': [861, 893], '(5Pattyn': [862], 'S.R.': [863], 'Colston': [864], 'M.J.': [865, 1538, 1653, 3338], 'Lepr.': [866, 992], 'Rev.': [867, 993], '1978;': [868], '49:': [869], '324-326Google': [870], 'Genome': [872], 'indeed': [874], 'shows': [875, 2798, 3151, 3230], 'despite': [877], 'dramatically': [879], 'reduced': [880, 3098, 3131, 3208], 'genome': [881, 894], 'size,': [882], 'ortholog': [886], 'conserved': [889], '(6Cole': [895], 'S.T.': [896, 991], 'Eiglmeier': [897], 'Parkhill': [899], 'James': [901], 'K.D.': [902], 'Thomson': [903], 'N.R.': [904], 'Wheeler': [905], 'P.R.': [906, 1124, 1188, 2768, 2944, 3414, 3628], 'Honore': [907], 'N.': [908, 936], 'Garnier': [909], 'T.': [910, 922, 932, 940, 1299], 'Churcher': [911], 'Harris': [913], 'D.': [914, 918, 920, 1038, 1098, 1301], 'Mungall': [915], 'Basham': [917], 'Brown': [919], 'Chillingworth': [921], 'Connor': [923], 'R.': [924, 970], 'Davies': [925], 'R.M.': [926], 'Devlin': [927], 'Duthoy': [929], 'Feltwell': [931], 'Fraser': [933], 'Hamlin': [935], 'Holroyd': [937], 'Hornsby': [939], 'Jagels': [941], 'Lacroix': [943], 'Maclean': [945], 'Moule': [947], 'Murphy': [949], 'L.': [950, 1026, 1030, 1034], 'Oliver': [951], 'Quail': [953], 'Rajandream': [955], 'Rutherford': [957], 'K.M.': [958], 'Rutter': [959], 'Seeger': [961], 'Simon': [963], 'Simmonds': [965], 'Skelton': [967], 'Squares': [969, 971], 'Stevens': [973], 'Taylor': [975], 'Whitehead': [977], 'Woodward': [979], 'J.R.': [980], 'Barrell': [981], 'Nature.': [983, 1101], '2001;': [984, 994, 1553, 1668, 3353, 3535], '409:': [985], '1007-1011Google': [986], '7Grosset': [988], 'J.H.': [989], 'Cole': [990, 1099], '72:': [995], '429-440Google': [996], 'Scholar).Recently,': [997], 'primary': [999], 'target': [1001, 1055, 1273, 1377], 'EtaA-activated': [1003], 'InhA,': [1010], 'enoyl-acyl': [1012], 'carrier': [1013], 'reductase': [1015], 'involved': [1016], 'fatty': [1018, 1404], 'acid': [1019], 'synthesis': [1020, 1401], '(8Larsen': [1021], 'M.H.': [1022, 1036], 'Vilcheze': [1023], 'Kremer': [1025], 'Parsons': [1029], 'Salfinger': [1031], 'Heifets': [1033], 'Hazbon': [1035], 'Alland': [1037], 'Sacchettini': [1039], 'Jacobs': [1041, 1305], 'Jr.,': [1042, 1306], 'W.R.': [1043, 1307], 'Mol.': [1044], 'Microbiol.': [1045, 1762, 2646], '46:': [1047], '453-466Google': [1048], 'InhA': [1050, 1274, 1287, 1325], 'another': [1057], 'known': [1059, 1519, 2810, 3074, 3644], 'prodrug,': [1061], 'isoniazid.': [1062], 'Although': [1063], 'sharing': [1064], 'same': [1066, 2887, 2998, 4144], 'target,': [1068], 'specifically': [1075, 1398], 'unrelated': [1079], 'redox': [1080, 2358, 4236, 4284], 'enzymes.': [1081], 'While': [1082], 'heme-containing': [1088], 'catalase-peroxidase': [1089], '(KatG)': [1090], '(9Zhang': [1091], 'Y.': [1092], 'Heym': [1093], 'B.': [1094, 1096, 1407, 2372, 3531, 3731], 'Allen': [1095], 'Young': [1097], '1992;': [1102], '358:': [1103], '591-593Google': [1104], 'Scholar),': [1105, 3874, 3914], 'recently': [1108, 1527, 1569], 'shown': [1110, 1711, 1719, 1830, 1838, 3924], 'flavin-containing': [1115, 1439, 1456, 2805, 3646], '(10Vannelli': [1117, 1181, 2761, 2937, 3407, 3621], 'T.A.': [1118, 1182, 2762, 2938, 3408, 3622], 'Dykman': [1119, 1183, 2763, 2939, 3409, 3623], 'Ortiz': [1121, 1185, 2765, 2941, 3411, 3625], 'de': [1122, 1186, 1302, 2381, 2766, 2942, 3412, 3626], 'Montellano': [1123, 1187, 2767, 2943, 3413, 3627], '277:': [1129, 1193, 2773, 2949, 3419, 3633, 3736], '12824-12829Google': [1130, 1194, 2774, 2950, 3420, 3634], 'EtaA-mediated': [1132, 1253, 3769, 4097], 'conversion': [1133, 3431, 3440, 3486, 3496, 3770, 4059, 4289], 'results': [1136, 1794, 3835], 'formation': [1139, 2285, 3714, 4068, 4083], 'sulfoxide': [1143, 1146, 3402], 'product.': [1144, 1368, 4073], 'does': [1148, 3487], 'not': [1149, 3014, 3425, 3437, 3488, 3829, 3943, 3952, 3977], 'represent': [1150], 'cytotoxic': [1152], 'species': [1153, 1208, 3004], 'but': [1154], 'rather': [1155], 'subsequent': [1162], 'reaction.': [1163], 'second': [1165, 2096], 'catalyzed': [1173, 3757], 'yielding': [1176, 3813], '2-ethyl-4-amidopyridine': [1177], 'amide': [1198], 'no': [1200, 2777], 'activity,': [1202], 'key': [1206], 'toxic': [1207, 1262], 'formed': [1210, 1249], 'unstable': [1213], 'reactive': [1214], 'intermediate': [1216, 1265], 'suggested': [1243], 'initial': [1246], 'sulfinate': [1247], 'consecutive': [1252], 'sulfoxidations': [1254], 'decomposes': [1257], 'form': [1259, 2841, 3160, 3854], 'labile': [1261], 'intermediate.': [1263], 'would': [1266], 'resemble': [1267], 'inhibiting': [1270], 'forming': [1276], 'covalently': [1278], 'modified': [1279], 'NADH': [1280, 1340], 'derivative': [1281], 'active': [1284, 2157, 3844], 'site': [1285, 1709, 1717, 1744, 1751, 1828, 1836, 3845], '(11Banerjee': [1288], 'Dubnau': [1290], 'E.': [1291, 1690, 1695, 1845], 'Quemard': [1292], 'Balasubramanian': [1294], 'V.': [1295, 2370], 'Um': [1296], 'K.S.': [1297], 'Wilson': [1298], 'Collins': [1300], 'Lisle': [1303], 'G.': [1304, 2376], '1994;': [1309], '263:': [1310], '227-230Google': [1311], 'Nevertheless,': [1313], 'isoniazid-based': [1329], 'inhibition': [1330, 1332], 'prevented': [1335], 'addition': [1338, 2740, 3662, 3821, 3973], 'NAD+': [1342], '(12Johnsson': [1343], 'King': [1345], 'D.S.': [1346], 'Schultz': [1347], 'P.G.': [1348], 'Am.': [1350], 'Soc.': [1352], '1995;': [1353], '117:': [1354], '5009-5010Google': [1355], 'finding': [1358], 'suggests': [1359, 1451], 'different': [1361], 'may': [1382], 'differ': [1383], 'each': [1385], 'prodrug.': [1387], 'Different': [1388], 'isoniazid,': [1392], 'compounds': [1397], 'affect': [1399], 'short-chain': [1403], 'acids': [1405], '(13Phetsuksiri': [1406], 'Baulard': [1408], 'Cooper': [1410], 'A.M.': [1411], 'Minnikin': [1412], 'D.E.': [1413], 'Douglas': [1414], 'J.D.': [1415], 'Antimicrob.': [1420], 'Agents': [1421], 'Chemother.': [1422], '1999;': [1423], '43:': [1424], '1042-1051Google': [1425], 'Scholar).A': [1426], 'homology': [1428, 1436, 2799], 'search': [1429, 1503], 'using': [1430, 1620, 1681, 1792, 1822, 1878, 2025, 2044, 2090, 2132, 2176, 2185, 2216, 2355, 2440, 2578, 2626, 3072, 3268, 3433, 3576], 'significant': [1435, 3261, 4157], 'monooxygenases.': [1440], 'fingerprint': [1449], '(FXGXXXHXXXW(P/D))': [1450], '(BVMO)': [1459], '1The': [1460], 'abbreviations': [1461, 1473], 'are:': [1463, 1475], 'BVMOBaeyer-Villiger': [1464, 1476], 'monooxygenaseEtaAethionamide': [1465, 1477], 'monooxygenaseBSAbovine': [1466, 1478], 'albuminGC-MSgas': [1468, 1480], 'chromatography-mass': [1469, 1481], 'spectrometryFADflavin': [1470, 1482], 'adenine': [1471, 1483], 'dinucleotide.1The': [1472], 'dinucleotide.': [1484], '(14Fraaije': [1485, 2818, 3289, 3900], 'M.W.': [1486, 1547, 1662, 1757, 2641, 2819, 3290, 3347, 3517, 3600, 3793, 3867, 3901], 'Kamerbeek': [1487, 2820, 3291, 3902], 'N.M.': [1488, 1536, 1651, 1753, 2637, 2821, 3292, 3336, 3510, 3593, 3786, 3860, 3903], 'van': [1489, 1539, 1543, 1654, 1658, 2822, 3293, 3339, 3343, 3513, 3527, 3596, 3727, 3789, 3863, 3904], 'Berkel': [1490, 1544, 1659, 2823, 3294, 3344, 3514, 3597, 3728, 3790, 3864, 3905], 'W.J.H.': [1491, 1545, 1660, 2824, 3295, 3345, 3515, 3598, 3729, 3791, 3865, 3906], 'Janssen': [1492, 1548, 1663, 1758, 2642, 2825, 3296, 3348, 3511, 3594, 3787, 3861, 3907], 'D.B.': [1493, 1549, 1664, 1759, 2643, 2826, 3297, 3349, 3512, 3595, 3788, 3862, 3908], 'FEBS': [1494, 2827, 3298, 3909], 'Lett.': [1495, 2828, 3299, 3910], '518:': [1497, 2830, 3301, 3912], '43-47Google': [1498, 2831, 3302, 3913], 'fact,': [1501, 3540, 3660], 'data': [1508], 'base': [1509], 'closest': [1513], 'homolog': [1514], '(24%': [1515], 'identity)': [1517], '4-hydroxyacetophenone': [1522, 1643], 'BVMO': [1525, 3892, 3936], 'cloned': [1529], 'expressed': [1531], 'our': [1533], 'laboratory': [1534], '(15Kamerbeek': [1535, 1650, 3335], 'Moonen': [1537, 1652, 3337], 'der': [1540, 1655, 3340], 'Ven': [1541, 1656, 3341], 'J.G.': [1542, 1657, 3342], 'Fraaije': [1546, 1661, 1756, 2640, 3346, 3516, 3599, 3792, 3866], 'Eur.': [1550, 1665, 3350], 'Biochem.': [1552, 1667, 3352], '268:': [1554, 1669, 3354], '2547-2557Google': [1555, 1670, 3355], 'Scholar)': [1556], 'prompted': [1558, 3999], 'us': [1559, 4000], 'specificity': [1564], 'EtaA.EXPERIMENTAL': [1573], 'PROCEDURESChemicals—Restriction': [1574], 'enzymes,': [1575], 'NADH,': [1576], 'NADPH,': [1577, 2312, 2322, 2500], 'glucose': [1578], 'oxidase': [1579, 2436, 3326, 3672], '(grade': [1580], 'II),': [1581], 'catalase,': [1583], 'were': [1584, 1591, 1602, 1610, 1618, 1637, 1849, 1887, 2050, 2142, 2159, 2197, 2214, 2343, 2350, 2453, 2505, 2573, 3114, 3942, 4130], 'obtained': [1585, 1638, 1646, 3135, 3166, 3225], 'Roche': [1587], 'Applied': [1588], 'Ketones': [1590], 'purchased': [1592], 'Acros,': [1594], 'Aldrich,': [1595], 'Lancaster.': [1597], 'NADP+,': [1598], '3-acetyl-NADP+,': [1599], '3-amino-NADP+': [1601], 'Fluka.': [1604], 'All': [1605, 2345], 'chemicals': [1609], 'commercially': [1612], 'available': [1613], 'analytical': [1614], 'grade.': [1615], 'DNA': [1616], 'samples': [1617, 2490], 'QIAQuick': [1622], 'gel': [1623, 2846, 3007], 'purification': [1625, 2729, 2894], 'kit': [1626, 2228], 'Qiagen.': [1628], 'Escherichia': [1629], 'coli': [1630, 1846], 'TOP10-competent': [1631], 'cells': [1632, 1848, 2049], 'pBAD/myc-HisA': [1635, 1724], 'vector': [1636], 'Invitrogen.': [1640], 'Purified': [1641], 'recombinant': [1642, 2687, 2719, 2957], 'described': [1648, 2912], 'previously': [1649, 2760, 2932], 'Scholar).Expression': [1671], 'Purification': [1673], 'Recombinant': [1675, 2655], 'EtaA—The': [1676], 'amplified': [1680], 'pMH29-containing': [1682], 'template': [1685], '(kindly': [1686], 'provided': [1687], 'Barry,': [1691], 'III': [1692], 'DeBarber,': [1696], 'National': [1697], 'Institutes': [1698], 'Health)': [1700], 'following': [1703, 1880, 2580], 'primers:': [1705], 'PETA1': [1706], '(5′-GGCACGGCATATGACCGAGCACCTCGACGTTGTC-3′,': [1707], 'NdeI': [1708, 1736, 1750, 1777], 'underlined)': [1712, 1831, 1839], 'PETA2': [1714], '(5′-GGCACGGAAGCTTAACCCCCACCGGGGCAGGCCTTTTGG-3′,': [1715], 'HindIII': [1716, 1827, 1835], 'underlined).': [1720], 'expression,': [1722, 1844], '(Invitrogen)': [1725], 'derived': [1726], 'expression': [1727], 'vector,': [1728], 'pBADNK,': [1729], 'original': [1735], 'sites': [1737], 'removed,': [1740], 'whereas': [1741, 2478, 3702], 'NcoI': [1743], 'replaced': [1747], '(16Kamerbeek': [1752, 2636], 'Olsthoorn': [1754, 2638], 'A.J.J.': [1755, 2639], 'Appl.': [1760, 2644], 'Environ.': [1761, 2645], '2003;': [1763, 2647, 3521, 3604, 3797, 3871], '69:': [1764, 2648], '419-426Google': [1765, 2649], 'After': [1767, 1917, 1958], 'amplification,': [1768], 'isolated': [1772], 'gel,': [1774], 'digested': [1775], 'HindIII,': [1779], 'ligated': [1781], 'behind': [1782], 'araBAD': [1784], 'promoter': [1785], 'NdeI/HindIII-digested': [1788], 'pBADNK-yielding': [1789], 'pBETA1.': [1790], 'Expression': [1791, 1809], 'pBETA1': [1793, 1821, 1852], 'production': [1797], 'containing': [1800, 1912, 1984, 1997, 2493, 2524, 2657], '25': [1801, 1873, 2353, 2448, 2511], 'additional': [1802], 'C-terminal': [1803], 'residues': [1804], '(c-myc': [1805], 'epitope': [1806, 2662], 'His6).': [1808], 'native': [1811, 2878], 'achieved': [1814], 'introducing': [1816], 'stop': [1818], 'codon': [1819], 'primers': [1824], 'PETA3': [1825], '(5′-GCCCCGGTGGGGGTTTGAAAGCTTGGGCCCGAAC-3′,': [1826], 'PETA4': [1833], '(5′-GTTCGGGCCCAAGCTTTCAAACCCCCACCGGGGC-3′,': [1834], 'pBETA2.': [1842], 'TOP10': [1847], 'transformed': [1850], 'pBETA2': [1854], 'grown': [1856], 'LB': [1858], 'medium': [1859], 'supplemented': [1860], '100': [1862, 1898, 2195], 'μg/ml': [1863, 2434], 'ampicillin': [1864], '0.02%': [1866], '(w/v)': [1867, 2410], 'arabinose': [1868], 'temperature': [1871, 2581], '°C.His-tagged': [1874], 'procedure.': [1881], 'Cells': [1882], '2-liter': [1885], 'culture': [1886], 'harvested': [1888, 2048], 'centrifugation': [1890], 'resuspended': [1892, 1946, 2051], 'TMAG': [1894, 1977, 1983, 1996, 2186], '(50': [1895], 'mm': [1896, 1899, 1902, 1905, 1986, 1999, 2055, 2059, 2062, 2092, 2123, 2137, 2306, 2311, 2324, 2337, 2341, 2414, 2495, 2499, 2526, 3105], 'Tris,': [1897], 'KCl,': [1900], '1': [1901, 1904, 1953, 2058, 2061, 2680, 3505], 'β-mercaptoethanol,': [1903, 2060], 'NaN3,': [1906, 2063], '10%': [1907, 2064, 2409], 'v/v': [1908, 2065], 'glycerol,': [1909, 2066], 'pH': [1910, 2067, 2126, 2308, 2416, 3107], '7.5,': [1911, 2417], '1%': [1913], '(v/v)': [1914], 'Triton': [1915, 2677, 2743, 2755], 'X-100).': [1916], 'sonication': [1918], 'centrifugation,': [1920], 'supernatant': [1923], 'added': [1925], 'nickel-agarose': [1927], 'affinity': [1928], 'column': [1929, 1943, 1961, 1971, 1974, 1980, 1994, 2016, 2075, 2108, 2153, 2182, 2201, 2630], 'material': [1930, 1944, 1962], 'capacity': [1933], '50': [1935, 2305, 2413, 2586, 3104], 'mg.': [1936], 'By': [1937, 4006, 4095, 4233, 4274], 'means': [1938], 'end-over-end': [1940], 'rotation,': [1941], 'extract': [1949, 2078], 'incubated': [1951, 2506], 'h': [1954, 2509], '4': [1956], '°C.': [1957, 2512, 2616], 'incubation,': [1959], 'washed': [1964, 1991], 'gravitational': [1966], 'flow.': [1967], 'Upon': [1968], 'washing': [1969], '10': [1973, 1979, 2591, 3574], 'volumes': [1975, 1981], '20': [1985, 2117, 2122], 'imidazole,': [1987, 2004], '400': [1998, 2291, 3405], 'imidazole.': [2000], 'To': [2001], 'remove': [2002], 'collected': [2006, 2099, 2533], 'fraction': [2008, 2101], 'applied': [2010, 2080, 2103, 2147], 'preequilibrated': [2013], 'Hi-prep': [2014], '26/10-desalting': [2015], '(Amersham': [2017, 2154, 2183], 'Biosciences).': [2018, 2155], 'Finally,': [2019], 'concentrated': [2023, 2143, 2162], 'Amicon': [2027], 'ultrafiltration': [2028, 2145, 2164], 'unit': [2029], 'equipped': [2030, 2565], '100-kDa': [2033], 'cut-off': [2034], 'filter': [2035], 'stored': [2037, 2167], '–80': [2039, 2169], '°C.Native': [2040], 'three': [2045], 'columns.': [2046], 'buffer': [2053, 2730], '(20': [2054], 'potassium': [2056, 2124, 2138], 'phosphate,': [2057, 2125], '7.5),': [2068], 'sonicated,': [2069], 'centrifuged.': [2071], 'first': [2074], 'step,': [2076, 2097], 'onto': [2081, 2104], 'Q-Sepharose': [2083], 'column.': [2084, 2569], 'eluted': [2089, 2131, 2203], '250': [2091], 'KCl.': [2093], 'ceramic': [2106], 'hydroxyapatite': [2107], '(2.5': [2109], '×': [2110, 2633], '12': [2111], 'cm,': [2112], 'Type': [2113], 'I,': [2114], 'particle': [2115], 'size': [2116], 'Bio-Rad)': [2119], 'equilibrated': [2120], '7.0.': [2127], 'gradient': [2134, 2584, 2601], '20–300': [2136], 'phosphate.': [2139], 'Active': [2140], 'fractions': [2141, 2158], '26/60': [2150], 'Superdex': [2151, 2178], '200': [2152, 2179, 2405, 2870], 'Again,': [2156], 'pooled': [2160], 'subsequently': [2166, 2438, 2539], '°C.Analytical': [2170], 'Methods—Analytical': [2171], 'size-exclusion': [2172], 'chromatography': [2173], 'performed': [2175, 2351, 2546, 2852], 'HR': [2180], '10/30': [2181], 'Biosciences)': [2184], 'without': [2189, 2575], '0.5': [2190, 2336, 2433], 'mg/ml': [2191], 'BSA.': [2192], 'Aliquots': [2193], 'μl': [2196], 'loaded': [2198], 'flow': [2206], '0.8': [2209], 'ml/min.': [2210], 'Apparent': [2211], 'molecular': [2212, 2225], 'masses': [2213], 'determined': [2215, 2260, 2363, 3044, 3443, 4131, 4147, 4223], 'calibration': [2218], 'curve': [2219], 'made': [2220, 2419], 'standards': [2222], 'mass': [2226, 2563, 2705, 2716], 'marker': [2227], 'Bio-Rad:': [2230], 'thyroglobulin': [2231], '(670': [2232], 'kDa);': [2233, 2238, 2242, 2246], 'γ': [2235], 'globulin': [2236], '(158': [2237], 'chicken': [2239], 'ovalbumin': [2240], '(44': [2241], 'equine': [2243], 'myoglobin': [2244], '(17': [2245], 'vitamin': [2248], 'B12': [2249], '(1.35': [2250], 'kDa).Enzyme': [2251], 'tested': [2257, 3916, 4214, 4302], 'spectrophotometrically': [2261], 'decrease': [2265], 'NADPH': [2267, 2342, 3102, 3146, 3197, 3221, 3250, 3288, 3307, 3317, 3325, 3370, 3568, 3671, 3696, 3956, 3982, 4341], '340': [2269, 3477], 'nm': [2270, 2272, 2292, 2294, 2972, 2983, 3080, 3406, 4262], '(ϵ340': [2271], '6.22': [2274], 'mm–1': [2275, 2297], 'cm–1).': [2276, 2298], 'measured': [2281, 2474, 3266, 4138], 'ethionamide-oxide': [2288], '(ϵ400': [2293], '1.0': [2296, 2314, 2323, 2519, 2525], 'Reaction': [2299], 'mixtures': [2300], '(1.0': [2301, 2491], 'ml)': [2302, 2492], 'typically': [2303, 2989, 3502, 4111], 'contained': [2304], 'Tris/HCl,': [2307, 2415, 3106], '8.5,': [2309], '0.2': [2310, 2340], 'μm': [2315, 2395, 2399, 2406, 2503, 3101, 3145, 3149, 3478, 3575], 'enzyme.': [2316, 4273], 'determining': [2318, 4234], 'Km': [2320, 3475, 3572, 4152], 'substrate.': [2329, 3580], 'measuring': [2331, 3397], 'additives,': [2335], 'used.': [2344], 'kinetic': [2348, 3445, 4170], 'measurements': [2349], '°C': [2354, 2589, 2597, 2606], 'air-saturated': [2356], 'buffers.The': [2357], 'potential': [2359], 'method': [2366], 'Massey': [2368], '(17Massey': [2369], 'Curti': [2371], 'Ronchi': [2373], 'Zanetti': [2375], 'Flavins': [2377], 'Flavoproteins.': [2379], 'Walter': [2380], 'Gruyter,': [2382], 'Berlin,': [2383], 'Germany1990:': [2384], '59-66Google': [2385], 'Enzyme': [2387], '(5': [2388], 'μm)': [2389, 3095], '2': [2394, 2502], 'viologen,': [2397], '5': [2398, 2494], 'phenosafranin': [2400], '(Em': [2401], '–252': [2403], 'mV),': [2404], 'xanthine,': [2407], 'glycerol': [2411], 'anaerobic': [2420], 'cuvette': [2423], 'flushing': [2425], 'nitrogen.': [2427], 'Reduction': [2428, 2469], 'initiated': [2430], 'xanthine': [2435], 'monitored': [2439, 2623, 3395], 'PerkinElmer': [2442], 'Lambda': [2443], 'Bio': [2444], '40': [2445], 'spectrophotometer': [2446], '(at': [2447], '°C).': [2449], 'Spectra': [2450, 3109], '(300–700': [2451], 'nm)': [2452], 'recorded': [2454, 3115], 'every': [2455], 'minute': [2456], 'until': [2457], 'reduction': [2459, 2480, 3090, 3227, 3263], 'both': [2461], 'dye': [2462, 2472], 'complete': [2466], '(∼1.5': [2467], 'h).': [2468], '520': [2476], 'nm,': [2477], 'followed': [2483, 2598], '404': [2485], 'nm.Product': [2486], 'Identification—For': [2487], 'identification,': [2489], 'substrate,': [2497, 3688, 3701, 4282], '2.5': [2498], '24': [2508], 'reaction': [2514, 4043], 'quenched': [2516], 'ml': [2520], 'ethyl': [2522, 2529], 'acetate': [2523, 2530], 'hexadecane.': [2527], 'layer': [2531], 'dried': [2535], 'over': [2536], 'MgSO4': [2537], 'analyzed': [2540], 'GC-MS.': [2542], 'GC-MS': [2543, 3968, 4007], 'Hewlett': [2549, 2559], 'Packard': [2550, 2560], 'HP': [2551, 2561], '5890': [2552], 'series': [2553], 'gas': [2555], 'chromatograph': [2556], '5971': [2562], 'spectrometer': [2564], 'HP-5': [2568], 'Samples': [2570], '(1': [2571], 'μl)': [2572], 'injected': [2574], 'derivatization': [2576], 'program:': [2582], '150': [2588, 2596, 2603, 3100], 'min,': [2592, 2609], '4-min': [2593], 'isothermal': [2594, 2613], '300': [2605, 2615], '3': [2608], 'finally': [2611], '12-min': [2612], 'methyl-p-tolylsulfide': [2621], 'GC': [2625], 'Chiraldesc': [2628], 'G-TA': [2629], '(30': [2631], 'm': [2632], '0.25': [2634], 'mm)': [2635], 'Scholar).RESULTSPurification': [2650], 'Spectral': [2652, 2952], 'Characterization': [2653], 'EtaA—EtaA': [2656], 'His6': [2659], 'c-myc': [2661], 'tag': [2663], 'C': [2666], 'terminus': [2667], 'cell': [2671], 'extracts': [2672], 'X-100.': [2678], 'From': [2679], 'liter': [2681], 'culture,': [2683], '∼15': [2684], 'mg': [2685], 'purified.': [2691], 'migrated': [2695], 'band': [2699], 'SDS-PAGE': [2701], '∼56': [2707], 'kDa.': [2708], 'value': [2710, 3075, 3469, 3582, 4177, 4190], 'close': [2712], 'theoretical': [2715], '(58,216': [2721], 'Da).': [2722], 'Elimination': [2723], 'detergent': [2726], 'resulted': [2731, 3027, 4065], 'yields': [2735], '(<10%': [2736], 'compared': [2737], '0.1%': [2742], 'X-100),': [2744], 'suggesting': [2745], 'membrane-associated.': [2750], 'Solubilization': [2751], 'X-100': [2756], 'clear': [2778], 'hydrophobic': [2779], 'segments': [2780], 'serve': [2790], 'membrane': [2793], 'anchor.': [2794], 'monooxygenases': [2806, 3283, 3501, 3647, 3782], 'monomeric': [2813], 'dimeric': [2815], 'soluble': [2816], 'proteins': [2817], 'Nonetheless,': [2833], 'tends': [2839], 'oligomers': [2842, 2867], 'evidenced': [2844], 'filtration': [2847, 2850], 'experiments.': [2848], 'Gel': [2849], 'experiments': [2851, 2911], 'showed': [2856, 2885, 2959, 3050], 'exists': [2860], 'mixture': [2863, 3827], 'ranging': [2868, 4029], '600': [2872], 'kDa.Compared': [2873], 'His-tagged': [2875, 2896], 'displayed': [2880], 'hydrodynamic': [2882], 'properties': [2883], 'below).': [2892], 'As': [2893], 'efficient,': [2900], 'variant': [2903], 'below.EtaA': [2913], 'brightly': [2918], 'yellow': [2919], 'contains': [2924, 3055], 'tightly': [2926], 'bound': [2927], 'cofactor,': [2929], 'FAD': [2936, 3078, 3184], 'spectrum': [2963, 3128, 3215, 3224], 'displaying': [2964, 3464, 3570], 'absorbance': [2965, 2978, 3399, 4259], 'maxima': [2966], '367': [2969], '451': [2971, 2982], '2).': [2974, 3199], 'ratio': [2976, 2992], '280': [2980], '16.': [2990], 'oligomeric': [3003, 3018], 'upon': [3006, 3089], 'filtration,': [3008], 'content': [3012], 'dependent': [3015], 'state.': [3019], 'Unfolding': [3020], '0.05%': [3025], 'SDS': [3026], 'rapid': [3030], 'release': [3031], 'allowing': [3036], 'quantification': [3037], 'released': [3040], 'cofactor.': [3041, 3062], 'Combining': [3042], 'subunit': [3048], 'concentrations': [3049], 'stoichiometric': [3056], '(1:1)': [3057], 'amounts': [3058, 4280], 'molar': [3064], 'extinction': [3065], 'coefficient': [3066], 'calculated': [3071], 'free': [3077], '(ϵ451': [3079], '11,600': [3083], 'm–1': [3084], 'cm–1).Fig.': [3085], '2Spectral': [3086], 'changes': [3087], '(6.3': [3094], 'anaerobically': [3097, 3192], '8.5.': [3108], '(from': [3110], 'top': [3111], 'bottom)': [3113], 't': [3117], '0,': [3119], '5,': [3120], '10,': [3121], '15,': [3122], '20,': [3123], '30': [3125, 3507], 'min.': [3126], 'fully': [3130, 3236], '(—)': [3133], 'dithionite.': [3138], 'Aerobic': [3139, 3304], 'incubation': [3140, 3219, 3826], '440': [3144, 4261], '430': [3148], 'oxidized': [3159, 3841, 4182, 4195, 4272, 4315], 'steady-state': [3162, 3444, 4128], '(·····,': [3164], 'spectra': [3165], '2-min': [3168], 'incubation).View': [3169], 'Large': [3170], 'Image': [3171], 'Figure': [3172], 'ViewerDownload': [3173], '(PPT)Catalytic': [3174], 'Properties': [3175], 'EtaA—To': [3177], 'probe': [3178], 'whether': [3179, 3752], 'EtaA-bound': [3183], 'participates': [3185], 'catalysis,': [3187], 'mixed': [3191], 'excess': [3195], 'slowly': [3207], 'nicotinamide': [3211], 'coenzyme.': [3212], 'Comparing': [3213], 'reached': [3216], '30-min': [3218], 'dithionite': [3229], 'almost': [3235], 'reduced.': [3237], 'fact': [3239], 'bind': [3246], 'react': [3248], 'folded': [3256], 'catalytically': [3258], 'competent.': [3259], 'No': [3260], 'when': [3267], 'NADH.': [3269], 'characterized': [3281], 'sequence-related': [3282], 'highly': [3285], 'specific': [3286], 'incubations': [3305], 'catalyzes': [3312, 3806], 'oxidation': [3315, 3451, 3992, 4098, 4105], '(0.0045': [3318], 's–1)': [3319], 'generating': [3320], 'hydrogen': [3321, 3657, 3712, 3740], 'peroxide.': [3322, 3658], 'BVMOs': [3334], '19Ryerson': [3357], 'C.C.': [3358], 'Ballou': [3359], 'D.P.': [3360], 'Walsh': [3361], 'Biochemistry.': [3363], '1982;': [3364], '21:': [3365], '2644-2655Google': [3366], 'Scholar).Using': [3367], 'substrates,': [3372, 4126, 4303], 'observed,': [3382], 'confirming': [3383, 3875], 'directly': [3394], 'Oxygen': [3422], 'concentration': [3423], 'oxygen-saturated': [3434], 'solutions': [3435], 'did': [3436, 3828, 3951, 3976], 'increase': [3438, 3953, 3978], 'rate.': [3441], 'parameters': [3446, 4129, 4171], 'suggest': [3452, 4216], 'poor': [3458], '0.027': [3471], 's–1': [3472, 3508], '(Table': [3479, 4023, 4132], 'I).': [3480, 4024], 'Such': [3481], 'seem': [3489], 'physiological': [3493], 'importance': [3494], 'rates': [3497], '(kcat)': [3498], '(18Kamerbeek': [3509, 3592, 3785, 3859], 'Adv.': [3518, 3601, 3794, 3868], 'Synth.': [3519, 3602, 3795, 3869], 'Cat.': [3520, 3603, 3796, 3870], '345:': [3522, 3605, 3798, 3872], '667-678Google': [3523, 3606, 3799, 3873], '20Duetz': [3525], 'W.A.': [3526], 'Beilen': [3528], 'J.B.': [3529], 'Witholt': [3530, 3730], 'Curr.': [3532], 'Opin.': [3533], 'Biotechnol.': [3534], '12:': [3536], '419-425Google': [3537], 'difficult': [3543], 'envisage': [3545], 'inefficient': [3549], 'initiate': [3553], 'bactericidal': [3555], 'efficient': [3566], 'recognition': [3569], 'oxygenating': [3579], 'those': [3586], 'related-monooxygenases': [3591], 'Scholar).Table': [3607], 'IIdentified': [3608], 'productsaProduct': [3613], 'Vannelli': [3618], 'et': [3619], 'al.': [3620], 'Open': [3636, 4199], 'table': [3637, 4200], 'new': [3640, 4203], 'tab': [3641, 4204], 'catalyzing': [3653], 'oxygenations': [3654], 'generate': [3656], 'certain': [3664], 'effector': [3665], 'molecules': [3666, 3697], 'accelerate': [3668], 'activity.': [3673], 'example,': [3675], '2-hydroxybiphenyl': [3678], '3-monooxygenase': [3679], 'Pseudomonas': [3681], 'azelaica': [3682], 'HBP1': [3683], 'uses,': [3684], 'depending': [3685], 'small': [3691, 3985], 'part': [3692], 'consumed': [3695], 'hydroxylate': [3699], 'electron': [3707], 'equivalents': [3708], 'peroxide': [3713, 3741], '(21Meyer': [3715], 'Schmid': [3717], 'Held': [3719], 'Westphal': [3721], 'A.H.': [3722], 'Rothlisberger': [3723], 'Kohler': [3725], 'H.P.': [3726], '5575-5582Google': [3737], 'Because': [3739], 'potent': [3744], 'oxidative': [3745], 'agent,': [3746], 'imperative': [3749], 'test': [3751, 4002], 'reactions': [3756, 4106], 'truly': [3761], 'enzyme-mediated.': [3762], 'examined': [3767], 'methyl-p-tolylsulfide,': [3772], 'sulfide': [3774, 3839, 3882], 'readily': [3777], 'accepted': [3778, 3944], 'flavin-dependent': [3781], 'oxygenation': [3809, 4221], 'sulfide,': [3812], 'S-sulfoxide': [3816], '(55%': [3817], 'enantiomeric': [3818], 'excess).': [3819], 'catalase': [3823], 'influence': [3830], 'enantioselectivity.': [3833], 'These': [3834], 'indicate': [3836], 'reacting': [3849], 'electrophilic': [3852], 'hydroperoxy': [3853], 'direct': [3877], 'oxidation.EtaA': [3883], 'Catalyzes': [3884], 'Reactions—Hinted': [3886], 'identifying': [3893], 'converted': [3927, 4020, 4039], 'BVMOs.': [3929], '(cyclohexanone,': [3938], 'acetophenone,': [3939], '4-hydroxyacetophenone)': [3941], 'Incubations': [3947], 'consumption': [3954], 'nor': [3957], 'lactone': [3961], 'ester': [3963, 4072, 4089], 'products': [3964, 4090], 'detected': [3966], 'analysis.': [3969], 'although': [3971], 'bicyclohept-2-en-6-one': [3975], 'oxidation,': [3983], 'amount': [3986], '(∼1%': [3987], 'conversion)': [3988], 'product,': [3993], '3-oxabicyclo[3.3.0]oct-6-en-2-one,': [3994], 'detected.': [3997, 4094], 'aliphatic': [4004], 'ketones.': [4005], 'analysis,': [4008], 'surprisingly': [4014], 'Besides': [4025], 'alkanones': [4028, 4054], '2-dodecanone,': [4033, 4058, 4081], 'phenyl': [4034], 'substituent-bearing': [4035], 'into': [4044], 'esters.': [4047], '2-octanone,': [4055], '2-decanone,': [4056, 4079], 'case': [4076], '3-octanone,': [4078], 'some': [4082, 4122], 'chemically': [4086], 'less': [4087], 'favored': [4088], 'this,': [4096], 'obeys': [4099], 'stereochemical': [4101], 'rule': [4102], 'where': [4107], 'oxygen': [4109], 'inserted': [4112], 'migration': [4114], 'most': [4117], 'substituted': [4118], 'alkyl': [4119], 'chain.': [4120], 'II).': [4133], 'Remarkably,': [4134], 'Only': [4150], 'varied': [4154], 'extent,': [4158], 'revealing': [4159], 'far.Table': [4168], 'IISteady-state': [4169], 'EtaASubstrateKmkcatFMTaThe': [4173], 'flavin-monitored': [4174, 4187, 4255], '(FMT)': [4176, 4189], 'percentage': [4180, 4193], 'turnover.μms-1%Ethionamide3400.02788Phenylacetone610.01790Benzylacetone5200.021912-Dodecanone2000.02394a': [4185], 'turnover.': [4198], 'shared': [4226], 'event': [4228], 'along': [4229], 'catalytic': [4231, 4320], 'cycle.': [4232], 'state': [4237, 4285], 'turnover,': [4242], 'established': [4246], 'comprises': [4249], 'rate-limiting': [4251], 'event.': [4252], 'experiments,': [4257], 'taken': [4264], 'indicator': [4267], 'incubating': [4275], 'saturating': [4279], 'benzylacetone,': [4292], 'phenylacetone,': [4293], '2-dodecanone': [4295], 'determined.': [4297], 'state,': [4316], 'catalysis.During': [4331], 'half-reaction,': [4334], 'hydride': [4336], 'transferred': [4338], 'coenzyme': [4342], 'th': [4344]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2160143801', 'counts_by_year': [{'year': 2024, 'cited_by_count': 4}, {'year': 2023, 'cited_by_count': 3}, {'year': 2022, 'cited_by_count': 4}, {'year': 2021, 'cited_by_count': 7}, {'year': 2020, 'cited_by_count': 7}, {'year': 2019, 'cited_by_count': 1}, {'year': 2018, 'cited_by_count': 4}, {'year': 2017, 'cited_by_count': 6}, {'year': 2016, 'cited_by_count': 5}, {'year': 2015, 'cited_by_count': 7}, {'year': 2014, 'cited_by_count': 12}, {'year': 2013, 'cited_by_count': 4}, {'year': 2012, 'cited_by_count': 8}], 'updated_date': '2024-12-13T08:49:44.912727', 'created_date': '2016-06-24'}
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