Simple Method for Reduction of Autofluorescence in Fluorescence Microscopy

Name: Work Video:
Duration: 3 min 30 s
Description: Autofluorescence of aldehyde-fixed neural tissue often complicates the use of fluorescence microscopy. Background fluorescence can be notably reduced or eliminated by irradiation with light before treatment with fluorescence probes, resulting in a higher contrast without adversely affecting the staining probabilities.
Michael Neumann; Detlef Gabel
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