Title: Influence of glutamine on transient and stable recombinant protein production in CHO and HEK-293 cells
Abstract: BackgroundGlutamine is an essential component in culture mediafor most of the mammalian cell lines. It is often used asanalternativesourceofenergybycells,alongwithglu-cose. Glutamine metabolism induces ammonia accumu-lation in cell culture. Elevated ammonia concentrationabove 2 mM has been shown to have negative impacton both cell growth and recombinant protein productiv-ity [1-4]. In this study we investigated the effects ofdecreased glutamine concentration in the medium forCHO-DG44 and HEK-293E cells during transient geneexpression (TGE). The rationale was to reduce ammoniaaccumulation in the culture, and consequently, improvecell viability and recombinant protein productivity.Materials and methods