Title: IDENTIFICATION, ISOLATION, AND DETERMINATION OF FLAVONES IN <i>ORIGANUM VULGARE</i> FROM MACEDONIAN FLORA
Abstract: Abstract Assay of flavone aglycones in extracts of Origanum vulgare from Macedonia including identification and isolation is performed. Hydrolyzed extracts were analyzed by thin layer chromatography (TLC) and high performance liquid chromatography (HPLC). Three aglycones were identified and then isolated by column chromatography and TLC. Further TLC, HPLC, and UV/VIS spectrophotometric analysis showed that the isolated compounds are luteolin (the most abundant), apigenin, and diosmetin. A new and rapid HPLC method was developed only for determination of the content of luteolin, which can serve as an appropriate criterion for evaluation of the flavonoid content in the drug. The accuracy of the method was proven by the method of standard additions, which gave satisfactory values for the recovery (98.2–100.3 %). The quantity of luteolin found in the drug ranged from 0.40–0.50 % (m/m). Acknowledgments
Publication Year: 2001
Publication Date: 2001-01-12
Language: en
Type: article
Indexed In: ['crossref']
Access and Citation
Cited By Count: 19
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot