Abstract: Angewandte Chemie International EditionVolume 50, Issue 50 p. 11942-11946 Communication Solution NMR Structure of Proteorhodopsin† Sina Reckel, Sina Reckel Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max-von-Laue Str. 9, 60438 Frankfurt (Germany) These authors contributed equally to this work.Search for more papers by this authorDaniel Gottstein, Daniel Gottstein Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max-von-Laue Str. 9, 60438 Frankfurt (Germany) These authors contributed equally to this work.Search for more papers by this authorJochen Stehle, Jochen Stehle Institute for Organic Chemistry and Chemical Biology and Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max-von-Laue Str. 7–9, 60438 Frankfurt (Germany)Search for more papers by this authorDr. Frank Löhr, Dr. Frank Löhr Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max-von-Laue Str. 9, 60438 Frankfurt (Germany)Search for more papers by this authorDr. Mirka-Kristin Verhoefen, Dr. Mirka-Kristin Verhoefen Institute of Physical and Theoretical Chemistry, Goethe University Frankfurt, Max-von-Laue Str. 7, 60438 Frankfurt (Germany)Search for more papers by this authorDr. Mitsuhiro Takeda, Dr. Mitsuhiro Takeda Structural Biology Research Center, Nagoya University, Furo-cho, Chikusa-ku, 464-8601 (Japan)Search for more papers by this authorRobert Silvers, Robert Silvers Institute for Organic Chemistry and Chemical Biology and Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max-von-Laue Str. 7–9, 60438 Frankfurt (Germany)Search for more papers by this authorProf. Dr. Masatsune Kainosho, Prof. Dr. Masatsune Kainosho Structural Biology Research Center, Nagoya University, Furo-cho, Chikusa-ku, 464-8601 (Japan) Center for Priority Areas, Tokyo Metropolitan University, Hachioji, Tokyo 192-0397 (Japan)Search for more papers by this authorProf. Dr. Clemens Glaubitz, Prof. Dr. Clemens Glaubitz Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max-von-Laue Str. 9, 60438 Frankfurt (Germany)Search for more papers by this authorProf. Dr. Josef Wachtveitl, Prof. Dr. Josef Wachtveitl Institute of Physical and Theoretical Chemistry, Goethe University Frankfurt, Max-von-Laue Str. 7, 60438 Frankfurt (Germany)Search for more papers by this authorDr. Frank Bernhard, Dr. Frank Bernhard Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max-von-Laue Str. 9, 60438 Frankfurt (Germany)Search for more papers by this authorProf. Dr. Harald Schwalbe, Prof. Dr. Harald Schwalbe Institute for Organic Chemistry and Chemical Biology and Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max-von-Laue Str. 7–9, 60438 Frankfurt (Germany)Search for more papers by this authorProf. Dr. Peter Güntert, Prof. Dr. Peter Güntert Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max-von-Laue Str. 9, 60438 Frankfurt (Germany) Frankfurt Institute for Advanced Studies, Goethe University Frankfurt, Ruth-Moufang-Str.1, 60438 Frankfurt am Main (Germany) Center for Priority Areas, Tokyo Metropolitan University, Hachioji, Tokyo 192-0397 (Japan)Search for more papers by this authorProf. Dr. Volker Dötsch, Corresponding Author Prof. Dr. Volker Dötsch [email protected] Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max-von-Laue Str. 9, 60438 Frankfurt (Germany)Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max-von-Laue Str. 9, 60438 Frankfurt (Germany)Search for more papers by this author Sina Reckel, Sina Reckel Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max-von-Laue Str. 9, 60438 Frankfurt (Germany) These authors contributed equally to this work.Search for more papers by this authorDaniel Gottstein, Daniel Gottstein Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max-von-Laue Str. 9, 60438 Frankfurt (Germany) These authors contributed equally to this work.Search for more papers by this authorJochen Stehle, Jochen Stehle Institute for Organic Chemistry and Chemical Biology and Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max-von-Laue Str. 7–9, 60438 Frankfurt (Germany)Search for more papers by this authorDr. Frank Löhr, Dr. Frank Löhr Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max-von-Laue Str. 9, 60438 Frankfurt (Germany)Search for more papers by this authorDr. Mirka-Kristin Verhoefen, Dr. Mirka-Kristin Verhoefen Institute of Physical and Theoretical Chemistry, Goethe University Frankfurt, Max-von-Laue Str. 7, 60438 Frankfurt (Germany)Search for more papers by this authorDr. Mitsuhiro Takeda, Dr. Mitsuhiro Takeda Structural Biology Research Center, Nagoya University, Furo-cho, Chikusa-ku, 464-8601 (Japan)Search for more papers by this authorRobert Silvers, Robert Silvers Institute for Organic Chemistry and Chemical Biology and Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max-von-Laue Str. 7–9, 60438 Frankfurt (Germany)Search for more papers by this authorProf. Dr. Masatsune Kainosho, Prof. Dr. Masatsune Kainosho Structural Biology Research Center, Nagoya University, Furo-cho, Chikusa-ku, 464-8601 (Japan) Center for Priority Areas, Tokyo Metropolitan University, Hachioji, Tokyo 192-0397 (Japan)Search for more papers by this authorProf. Dr. Clemens Glaubitz, Prof. Dr. Clemens Glaubitz Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max-von-Laue Str. 9, 60438 Frankfurt (Germany)Search for more papers by this authorProf. Dr. Josef Wachtveitl, Prof. Dr. Josef Wachtveitl Institute of Physical and Theoretical Chemistry, Goethe University Frankfurt, Max-von-Laue Str. 7, 60438 Frankfurt (Germany)Search for more papers by this authorDr. Frank Bernhard, Dr. Frank Bernhard Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max-von-Laue Str. 9, 60438 Frankfurt (Germany)Search for more papers by this authorProf. Dr. Harald Schwalbe, Prof. Dr. Harald Schwalbe Institute for Organic Chemistry and Chemical Biology and Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max-von-Laue Str. 7–9, 60438 Frankfurt (Germany)Search for more papers by this authorProf. Dr. Peter Güntert, Prof. Dr. Peter Güntert Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max-von-Laue Str. 9, 60438 Frankfurt (Germany) Frankfurt Institute for Advanced Studies, Goethe University Frankfurt, Ruth-Moufang-Str.1, 60438 Frankfurt am Main (Germany) Center for Priority Areas, Tokyo Metropolitan University, Hachioji, Tokyo 192-0397 (Japan)Search for more papers by this authorProf. Dr. Volker Dötsch, Corresponding Author Prof. Dr. Volker Dötsch [email protected] Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max-von-Laue Str. 9, 60438 Frankfurt (Germany)Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max-von-Laue Str. 9, 60438 Frankfurt (Germany)Search for more papers by this author First published: 27 October 2011 https://doi.org/10.1002/anie.201105648Citations: 153 † This work was supported by the Deutsche Forschungsgemeinschaft (grant number DO545/7-1 and SFB 807), the European Drug Initiative on Channels and Transporters (EDICT) contract number HEALTH-F4-2007-201924, the NIH (grant number U54 GM094608), the Center for Biomolecular Magnetic Resonance (BMRZ) and the Cluster of Excellence Frankfurt (Macromolecular Complexes). The [11,20-13C2]retinal was a kind gift of Johan Lugtenburg, Peter Verdegem (Leiden University), Neville McLean, Malcolm H. Levitt and Richard C. D. Brown (Southampton University). P.G. gratefully acknowledges financial support by the Lichtenberg program of the Volkswagen Foundation and by a Grant-in-Aid for Scientific Research of the Japan Society for the Promotion of Science. Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Graphical Abstract A solved puzzle: The structure of the seven-transmembrane-helix proton pump proteorhodopsin obtained by solution NMR spectroscopy is based on NOE data combined with distance restraints derived from paramagnetic relaxation enhancement (see picture). Restraints from residual dipolar couplings improved the structural accuracy. Supporting Information Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Filename Description anie_201105648_sm_miscellaneous_information.pdf1.1 MB miscellaneous_information Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. 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