Publication Information

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5340, 5403, 5411, 5478, 5494, 5504, 5570, 5577, 5735, 5748, 5757, 5780, 5834, 5999, 6010, 6017, 6035], 'amyloid': [4, 21, 201, 218, 396, 417, 449], 'β-peptide': [5, 16, 202, 213, 418, 450], 'is': [6, 17, 64, 151, 191, 203, 214, 261, 348, 388, 508, 583, 621, 986, 1017, 1196, 2192, 2422, 2466, 2560, 2582, 2608, 2632, 2677, 2882, 2899, 2907, 2921, 2928, 2938, 2946, 2970, 3005, 3080, 3108, 3159, 3202, 3211, 4082, 4198, 4695, 4794, 4943, 5082, 5096, 5194, 5206, 5225, 5237, 5249, 5261, 5267, 5305, 5497, 5750, 5761, 5774, 5830], 'an': [7, 192, 204, 389, 514, 987, 1018, 1197, 1251, 1738, 1987, 2067, 2468, 2575, 4944, 5226, 5310], 'early': [8, 205, 490, 515], 'and': [9, 32, 34, 105, 112, 128, 138, 179, 206, 229, 231, 302, 309, 325, 335, 376, 433, 506, 516, 631, 653, 692, 706, 745, 1005, 1008, 1027, 1092, 1110, 1130, 1159, 1176, 1250, 1334, 1390, 1449, 1542, 1650, 1744, 1782, 1791, 1818, 1847, 1879, 1906, 1967, 2028, 2042, 2154, 2255, 2348, 2351, 2671, 2685, 2842, 2858, 2891, 2923, 3025, 3114, 3130, 3275, 3298, 3307, 3368, 3529, 3556, 3588, 3594, 3683, 3868, 3874, 3892, 3955, 4010, 4019, 4078, 4134, 4157, 4205, 4216, 4219, 4236, 4277, 4283, 4317, 4352, 4403, 4491, 4524, 4623, 4627, 4702, 4724, 4751, 4769, 4936, 5198, 5279, 5499, 5710, 5915], 'critical': [10, 207, 517], 'feature': [11, 208], "Alzheimer's": [13, 210, 394, 426, 6036], 'disease.': [14, 211], 'Amyloid': [15, 212], 'released': [18, 215, 2265], 'from': [19, 154, 216, 351, 1032, 1041, 1048, 1053, 1060, 1067, 1523, 1807, 1821, 2054, 2266, 2490, 2566, 2691, 2717, 2730, 2755, 2793, 3370, 3627, 3704, 3765, 3858, 3865, 3904, 3985, 4180, 4359, 4413, 4434, 4459, 4625, 4631, 4820], 'the': [20, 25, 54, 75, 90, 119, 131, 134, 139, 148, 164, 168, 173, 180, 217, 222, 251, 272, 287, 316, 328, 331, 336, 345, 361, 365, 370, 377, 447, 470, 482, 577, 591, 594, 648, 657, 662, 693, 701, 704, 740, 756, 763, 979, 998, 1003, 1009, 1022, 1093, 1111, 1189, 1228, 1241, 1264, 1271, 1335, 1394, 1443, 1450, 1554, 1585, 1593, 1693, 1718, 1745, 1863, 1915, 1919, 1940, 1971, 2078, 2094, 2105, 2110, 2124, 2185, 2358, 2426, 2432, 2438, 2456, 2459, 2491, 2567, 2572, 2595, 2606, 2615, 2625, 2640, 2663, 2668, 2672, 2741, 2748, 2765, 2772, 2777, 2800, 2819, 2824, 2835, 2846, 2855, 2861, 2878, 2896, 2924, 2941, 2957, 2966, 2974, 2985, 3003, 3018, 3027, 3035, 3041, 3046, 3068, 3075, 3083, 3089, 3103, 3112, 3115, 3121, 3134, 3144, 3151, 3167, 3191, 3205, 3208, 3278, 3300, 3308, 3323, 3327, 3331, 3342, 3347, 3371, 3429, 3436, 3447, 3461, 3530, 3623, 3642, 3655, 3679, 3684, 3689, 3847, 3859, 3878, 3881, 3884, 3889, 3893, 3896, 3905, 3917, 3939, 3978, 3986, 3998, 4007, 4011, 4029, 4044, 4063, 4090, 4099, 4102, 4125, 4142, 4153, 4160, 4165, 4181, 4185, 4209, 4220, 4244, 4251, 4288, 4314, 4328, 4365, 4370, 4379, 4393, 4398, 4406, 4435, 4460, 4501, 4504, 4509, 4514, 4534, 4537, 4543, 4548, 4608, 4611, 4637, 4651, 4669, 4690, 4729, 4732, 4736, 4774, 4783, 4786, 4790, 4799, 4805, 4815, 4834, 4873, 4903, 4907, 4914, 4925, 4932, 4940, 5071, 5090, 5175, 5203, 5219, 5276, 5288, 5291, 5295, 5301, 5337, 5401, 5412, 5475, 5488, 5495, 5501, 5580, 5586, 5720, 5728, 5745, 5771, 5787, 5835, 5955, 5996, 6000, 6011, 6018, 6033, 6039], 'precursor': [22, 219, 397, 579], 'protein': [23, 68, 220, 265, 398, 580, 991, 1520, 1532, 1536, 1636, 1719, 1865, 2087, 2187, 2197, 2457, 2471, 2934, 3020, 3078, 3168, 3197, 3280, 3293, 3329, 3359, 3375, 3406, 3647, 3950, 4186, 4436, 4461, 4911, 4942, 4947, 5193, 5224], 'by': [24, 221, 610, 627, 1123, 1423, 1426, 1562, 1620, 1749, 1764, 1843, 1926, 2098, 2120, 2140, 2174, 2180, 2354, 2740, 2764, 2780, 2978, 3053, 3353, 3374, 3380, 3411, 4270, 4305, 4343, 4427, 4452, 4580, 4661, 4828, 4867, 4921, 5812], 'sequential': [26, 223], 'action': [27, 224], 'two': [29, 226, 633, 3348, 4075, 4154, 4233, 4280, 4349, 4848, 4887, 5772], 'proteases,': [30, 147, 227, 344], 'β-secretase': [31, 228, 643, 765, 3304, 4910, 5188, 5505, 5994, 6022], 'γ-secretase,': [33, 230], 'these': [35, 232, 615, 4107, 4138, 4681], 'proteases': [36, 233, 5908, 5919], 'are': [37, 234, 430, 637, 1073, 3351, 4067, 4226, 4432, 4457, 4633, 4658, 4776, 5825], 'prime': [38, 235, 6030], 'targets': [39, 236], 'for': [40, 93, 237, 290, 639, 1117, 1133, 1147, 1182, 1255, 1387, 1393, 1613, 1643, 1665, 1731, 1885, 1930, 2086, 2178, 2796, 2839, 3064, 3099, 3288, 3846, 3911, 3922, 3928, 3938, 4061, 4212, 4610, 5315, 5585, 6021, 6032, 6048], 'therapeutic': [41, 238], 'intervention.': [42, 239], 'We': [43, 78, 240, 275, 982, 1001, 2338, 2951, 3171, 3876, 4495, 4528, 4605, 4917, 5211, 5730], 'have': [44, 79, 241, 276, 617, 696, 972, 3172, 4606, 4685, 4747, 4809, 5901], 'recently': [45, 242, 739, 754, 4908], 'cloned': [46, 243], 'a': [47, 81, 94, 176, 244, 278, 291, 373, 571, 606, 624, 1155, 1160, 1401, 1417, 1430, 1543, 1566, 1627, 1684, 1707, 1769, 1804, 1946, 1991, 2019, 2047, 2193, 2484, 2635, 2653, 2699, 2703, 2830, 2873, 2999, 3007, 3054, 3163, 3175, 3215, 3339, 3391, 3415, 3419, 3579, 3652, 3664, 3966, 4085, 4112, 4258, 4470, 4557, 4591, 4711, 4852, 4891, 4897, 5086, 5231, 5713, 5832, 5902, 5964, 6014], 'novel': [48, 245, 757], 'aspartic': [49, 146, 158, 246, 343, 355, 759, 5907, 5966], 'protease,': [50, 247], 'BACE,': [51, 248, 5479, 5742], 'with': [52, 144, 249, 341, 687, 1107, 1207, 1260, 1531, 1597, 1658, 1687, 1737, 1803, 1855, 1881, 1895, 1914, 1974, 2002, 2018, 2077, 2152, 2271, 2357, 2441, 2571, 2610, 2634, 2655, 2724, 2860, 2956, 2973, 3034, 3082, 3088, 3096, 3110, 3123, 3277, 3418, 3428, 3446, 3458, 3546, 3583, 3602, 3654, 3953, 4017, 4130, 4189, 4364, 4593, 4668, 5949, 5993], 'all': [53, 250, 485, 3982, 3993, 4177, 4645, 5799, 5821], 'known': [55, 145, 252, 342, 486], 'properties': [56, 253, 702, 4923], 'β-secretase.': [58, 255], 'Here': [59, 256, 976], 'we': [60, 257, 752, 977, 2828, 3282, 3335, 4140, 4169, 4648, 4937, 5408, 5739, 5805, 5818, 5941], 'demonstrate': [61, 258, 1014], 'that': [62, 69, 88, 155, 189, 259, 266, 285, 352, 386, 510, 563, 619, 632, 984, 992, 1015, 2190, 2260, 2420, 2464, 2496, 2624, 2906, 2935, 2945, 3017, 3026, 3079, 3107, 3139, 3180, 3204, 3563, 3992, 4101, 4650, 4666, 4680, 4804, 4930, 4939, 5080, 5259, 5265, 5280, 5287, 5718, 5744, 5756, 5820, 5898, 5943], 'BACE': [63, 86, 190, 260, 283, 387, 761, 980, 985, 1016, 1086, 1195, 1232, 1249, 1274, 1446, 2186, 2191, 2347, 2359, 2421, 2465, 2477, 2559, 2644, 2690, 2722, 2729, 2754, 2778, 2792, 2822, 2920, 3019, 3155, 3179, 3209, 3564, 3702, 3863, 3923, 3999, 4626, 4657, 4912, 4929, 4941, 5074, 5081, 5182, 5192, 5236, 5248, 5260, 5272, 5341, 5404, 5571, 5781, 5804, 5829], 'anN-glycosylated': [65, 262], 'integral': [66, 263, 989, 1198, 2469, 4945], 'membrane': [67, 264, 990, 1157, 1201, 2267, 2352, 2427, 2439, 2470, 4946], 'undergoes': [70, 267, 993, 3021], 'constitutive': [71, 268, 994, 3022], 'N-terminal': [72, 269, 995, 1252, 3023, 3028, 3128, 3852, 3860, 3975], 'processing': [73, 270, 596, 996, 1004, 2684, 3024, 3029, 3129, 5095, 5180, 5285, 5303], 'in': [74, 118, 271, 315, 519, 586, 602, 605, 997, 1101, 1168, 1405, 1414, 1577, 1591, 1601, 1668, 1724, 1869, 1898, 1961, 2425, 2431, 2745, 2769, 2799, 2823, 2833, 2845, 2948, 2995, 3031, 3045, 3067, 3143, 3156, 3214, 3319, 3345, 3362, 3620, 3997, 4069, 4164, 4200, 4228, 4405, 4469, 4556, 4778, 4796, 4896, 5075, 5174, 5294, 5405, 5954, 5960, 5995], 'Golgi': [76, 273, 999, 3047, 3145, 5296], 'apparatus.': [77, 274, 1000, 3048, 3146, 5297], 'used': [80, 277, 1408], 'secreted': [82, 279], 'Fc': [83, 280, 1451, 1548, 1555, 3309, 3333, 4166, 4380, 4407], 'fusion-form': [84, 281], '(BACE-IgG)': [87, 284], 'contains': [89, 106, 286, 303, 3299, 3565, 4931, 5275, 5727, 5782], 'entire': [91, 288, 5476], 'ectodomain': [92, 120, 289, 317, 1444, 3210, 4000, 5477, 5779], 'detailed': [95, 292, 6015], 'analysis': [96, 293, 2039, 2062, 2074, 2084, 2103, 2137, 2179, 3845, 3961, 4204, 4307, 4344, 4643, 4731, 4920], 'posttranslational': [98, 295, 3152], 'modifications.': [99, 296], 'This': [100, 160, 297, 357, 749, 2648, 3049, 3291, 3432, 3989, 4264, 4291, 4900], 'molecule': [101, 298, 3340], 'starts': [102, 299, 3832], 'at': [103, 300, 623, 1127, 1136, 1150, 1178, 1409, 1610, 1662, 1734, 1836, 1845, 1888, 2007, 2104, 2481, 2579, 2618, 2854, 3006, 3394, 3466, 3534, 3675, 3686, 3834, 4299, 4833, 4872, 4913, 5215, 5716, 6038], 'Glu46': [104, 301, 5581], 'fourN-glycosylation': [107, 304], 'sites': [108, 305, 1007, 2092, 3567, 4619, 4655, 4683, 4825, 4864], '(Asn153,': [109, 306, 4620], 'Asn172,': [110, 307, 4621], 'Asn223,': [111, 308, 4622], 'Asn354).': [113, 310], 'six': [115, 312, 5783], 'Cys': [116, 313, 1253, 4477], 'residues': [117, 137, 314, 334, 1590, 3039, 3866, 3944, 3996, 4423, 4448], 'form': [121, 318, 473, 2905, 2927, 2944, 3106, 3177, 4001, 4503, 4536, 4927, 5088, 5493, 5588, 5737, 5800], 'three': [122, 319, 5822], 'intramolecular': [123, 320, 5826], 'disulfide': [124, 149, 182, 321, 346, 379, 1010, 3355, 4002, 4008, 4126, 4144, 4178, 4214, 4222, 4265, 4338, 4377, 4505, 4538, 4853, 4892, 5801, 5823, 5956], 'linkages': [125, 322, 4127, 4215, 4400], '(Cys216–Cys420,': [126, 323], 'Cys278–Cys443,': [127, 324], 'Cys330–Cys380).': [129, 326], 'Despite': [130, 327], 'conservation': [132, 329], 'active': [135, 332, 2261, 3898, 5498], 'site': [136, 333, 4629], '30–37%': [140, 337], 'amino': [141, 338, 1245, 1457, 2065, 2492, 3312, 4091, 4671], 'acid': [142, 339, 405, 472, 1976, 2005, 2493, 4092, 4672], 'homology': [143, 340, 5948], 'motif': [150, 347], 'fundamentally': [152, 349], 'different': [153, 350, 634, 5764], 'other': [157, 354, 5768, 5906, 5950], 'proteases.': [159, 356], 'difference': [161, 358, 4312], 'may': [162, 359, 2498, 4319, 4684, 4808, 6006, 6042], 'affect': [163, 360, 6007], 'substrate': [165, 362, 713, 6008], 'specificity': [166, 363, 5339, 5503, 6009], 'enzyme.': [169, 366, 3644], 'Taken': [170, 367, 613], 'together,': [171, 368, 614], 'both': [172, 369, 743, 3111, 3127, 4122], 'presence': [174, 371, 2801, 3940, 4161], 'transmembrane': [177, 374, 575, 758, 2195, 3192, 4934, 5489, 5587], 'domain': [178, 375, 2196, 3302, 3861], 'unusual': [181, 378, 1019], 'bond': [183, 380, 4223, 4266], 'structure': [184, 381, 3206, 3343, 3437, 3449, 4793, 5903, 6019], 'lead': [185, 382], 'us': [186, 383, 4294], 'to': [187, 384, 480, 646, 661, 698, 1140, 1218, 1227, 1552, 1583, 1640, 1681, 1683, 1702, 1811, 1825, 1909, 2109, 2639, 3149, 3161, 3169, 3284, 3337, 3871, 3909, 3924, 4027, 4083, 4089, 4175, 4255, 4295, 4322, 4334, 4384, 4507, 4541, 4636, 4728, 4782, 4814, 5085, 5186, 5566, 5763, 5786, 5905, 6045], 'conclude': [188, 385], 'atypical': [193, 390], 'pepsin': [194, 391, 1023, 1759, 4190, 5316, 5836, 5951], 'family': [195, 392, 5837, 5952], 'member.': [196, 393], 'disease': [395, 427, 6037], 'β-site': [399], 'APP-cleaving': [400], 'enzyme': [401, 2095, 2262, 3885, 5304, 5496], 'fluorescein': [402], '5-maleimide': [403, 1037], 'α-cyano-4-hydroxycinnamic': [404], 'high': [406, 1075, 4810], 'performance': [407], 'liquid': [408], 'chromatography': [409], 'matrix-assisted': [410], 'laser': [411, 1934], 'desorption': [412, 1935], 'ionization': [413, 1936], 'trypsin-endoproteinase': [414, 4414], 'Asp-N': [415, 1029, 1689, 4415], '(3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate': [416], 'polyacrylamide': [419], 'gel': [420, 1563, 3381, 3581], 'electrophoresis': [421], 'phenylthiohydantoin': [422], 'hallmarks': [424], '(AD)1': [428], 'pathology': [429], 'brain': [431, 3767], 'plaques': [432], 'vascular': [434], 'deposits': [435], '(1.Alzheimer': [436], 'A.': [437, 541, 1351, 3321, 5977, 5979], 'Centralbl.': [438], 'Nervenheilk.': [439], 'Psychiatr.': [440], '1907;': [441], '30:': [442], '177-179Google': [443], 'Scholar)': [444, 562, 735, 971, 1333, 2418, 3274, 3763, 3831, 5473, 5709], 'consisting': [445, 4151], '4-kDa': [448], '(Aβ)': [451], '(2.Glenner': [452], 'G.G.': [453], 'Wong': [454], 'C.W.': [455], 'Biochem.': [456], 'Biophys.': [457, 5321, 5322, 5928, 5929], 'Res.': [458], 'Commun.': [459], '1984;': [460], '120:': [461], '885-890Crossref': [462], 'PubMed': [463, 501, 558, 681, 731, 818, 864, 928, 967, 1329, 1368, 1514, 2165, 2250, 2333, 2414, 2553, 3270, 3524, 3759, 3827, 5000, 5064, 5167, 5327, 5394, 5469, 5558, 5641, 5705, 5890, 5934, 5985], 'Scopus': [464, 502, 559, 682, 732, 819, 865, 929, 968, 1330, 1369, 1515, 2166, 2251, 2334, 2415, 2554, 3271, 3525, 3760, 3828, 5001, 5065, 5168, 5328, 5395, 5470, 5559, 5642, 5706, 5891, 5935, 5986], '(4225)': [465], 'Google': [466, 504, 561, 684, 734, 821, 867, 931, 970, 1332, 1371, 1517, 2168, 2253, 2336, 2417, 2556, 3273, 3527, 3762, 3830, 5003, 5067, 5170, 5330, 5397, 5472, 5561, 5644, 5708, 5893, 5937, 5988], 'Scholar).': [467, 685, 822, 1372, 1518, 2169, 2337, 2557, 5068, 5171, 5398, 5938], 'Overproduction': [468], '42-amino': [471], 'Aβ,': [475, 652], 'Aβ42,': [476], 'has': [477, 527, 599, 2257, 2889, 2987, 3294, 4735, 5104, 5719, 5945], 'been': [478, 600, 2258, 3295, 5105], 'suggested': [479], 'be': [481, 2264, 2499, 3186, 3983, 4084, 4320, 4385, 5482, 6046], 'cause': [483], 'cases': [487], 'familial': [489], 'onset': [491], 'AD': [492, 521], '(3.Younkin': [493], 'S.G.': [494], 'J.': [495, 537, 791, 795, 809, 888, 896, 906, 918, 923, 1302, 1306, 1320, 1487, 1491, 1505, 2223, 2227, 2241, 2293, 2301, 2311, 2323, 2328, 2387, 2391, 2405, 2526, 2530, 2544, 3243, 3247, 3261, 3497, 3501, 3515, 3732, 3736, 3750, 3787, 3795, 3805, 3817, 3822, 4973, 4977, 4991, 5024, 5032, 5042, 5054, 5059, 5127, 5135, 5145, 5157, 5162, 5367, 5371, 5385, 5442, 5446, 5460, 5531, 5535, 5549, 5614, 5618, 5632, 5665, 5673, 5683, 5695, 5700, 5863, 5867, 5881, 5969, 5973, 5981], 'Physiol.': [496], '(Paris).': [497], '1998;': [498, 2162], '92:': [499], '289-292Crossref': [500], '(252)': [503], 'Scholar),': [505, 2254, 3528, 5331, 5562, 5894, 5989], 'it': [507, 533, 2256, 2497, 3158, 3201, 5563, 5790, 5899, 5944], 'assumed': [509, 3203], 'Aβ42': [511], 'plays': [513], 'role': [518], 'sporadic': [520], 'as': [522, 762, 1239, 1400, 1979, 2667, 2901, 2972, 3317, 3469, 3668, 3880, 3895, 3926, 4128, 4146, 4595, 5103, 5208, 5244, 5313, 5753, 5755], 'well.': [523, 5245], 'Therefore,': [524], 'Aβ': [525, 566, 620, 640, 665], 'metabolism': [526], 'attracted': [528], 'considerable': [529], 'interest.': [530], 'In': [531, 1550, 1581, 3092, 3147], '1987': [532], 'was': [534, 747, 1039, 1047, 1051, 1065, 1099, 1145, 1233, 1376, 1407, 1459, 1521, 1559, 1595, 1638, 1679, 1722, 1747, 1834, 1841, 1867, 1924, 1942, 1959, 1999, 2013, 2044, 2071, 2075, 2138, 3051, 3360, 3408, 3576, 3649, 3661, 3919, 3936, 3951, 4015, 4025, 4053, 4187, 4247, 4267, 4302, 4330, 4341, 4362, 4382, 4578, 4588], 'shown': [535, 618, 2259, 3318, 4199, 4227, 5959], '(4.Kang': [536], 'Lemaire': [538], 'H.-G.': [539], 'Unterbeck': [540], 'Salbaum': [542], 'J.M.': [543], 'Masters': [544], 'C.L.': [545], 'Grzeschik': [546], 'K.-H.': [547], 'Multhaup': [548], 'G.': [549, 811, 857, 904, 1322, 1507, 2243, 2309, 2407, 2546, 3263, 3517, 3752, 3803, 4993, 5040, 5143, 5387, 5462, 5551, 5634, 5681, 5883], 'Beyreuther': [550], 'K.': [551, 890, 2295, 3789, 5026, 5129, 5667], 'Muller-Hill': [552], 'B.': [553], 'Nature.': [554, 924, 963, 1364, 2329, 3823, 5060, 5163, 5701], '1987;': [555], '325:': [556], '733-736Crossref': [557], '(3951)': [560], 'formation': [564], 'requires': [567], 'proteolytic': [568, 595, 635, 1641], 'cleavage': [569, 3141, 3887, 5289, 5747], 'large': [572, 3164], 'type': [573], 'I': [574], 'protein,': [576, 1202], 'β-amyloid': [578], '(APP),': [581], 'which': [582, 2834, 3346, 4499, 4532, 5273, 5480], 'constitutively': [584, 5083], 'expressed': [585, 3361], 'most': [587, 628, 2983], 'cell': [588, 1266, 2340, 2826], 'types.': [589], 'Over': [590], 'next': [592], 'decade': [593], 'APP': [598, 645, 691], 'studied': [601], 'great': [603], 'detail': [604], 'variety': [607], 'systems': [609, 1779], 'many': [611], 'groups.': [612], 'studies': [616, 827], 'generated': [622], 'low': [625, 1544, 5240], 'rate': [626, 1833], 'cells': [629, 689, 1080, 1083, 1094, 1112, 1383, 1530, 2344, 2593, 2658, 2693, 2711, 2719, 2734, 2759, 2794, 2836, 3094, 3122, 3367, 3706, 5078, 5177], 'analyzed': [630, 1422, 1985, 2119, 3662, 4221, 4607, 5740], 'activities': [636], 'required': [638], 'generation.': [641], 'First,': [642], 'cleaves': [644, 656], 'generate': [647], 'N': [649], 'terminus': [650], 'second,': [654], 'γ-secretase': [655, 746], 'C': [658], 'terminus,': [659], 'leading': [660, 4813], 'release': [663, 1219, 2455], '(for': [666, 5920], 'review': [667, 5921], 'see': [668, 2992, 5922], 'Ref.': [669, 717, 5923], '5.Haass': [670], 'C.': [671, 839, 855, 1343, 1349], 'Selkoe': [672, 722, 1362], 'D.J.': [673, 723, 1363], 'Cell.': [674, 859], '1993;': [675], '75:': [676], '1039-1042Abstract': [677], 'Full': [678, 728], 'Text': [679, 729], 'PDF': [680, 730], '(740)': [683], 'Studies': [686], 'intact': [688, 1651, 4926], 'expressing': [690, 1085, 2713, 2721], 'endogenous': [694], 'secretases': [695], 'led': [697], 'conclusions': [699], 'about': [700], 'β-': [705, 744], 'γ-secretases,e.g.': [707], 'their': [708], 'tissue': [709], 'distribution,': [710], 'subcellular': [711], 'localization,': [712], 'requirements': [714], '(see': [715, 3930, 4273, 4389, 4438, 4463, 4517, 4551], 'e.g.': [716, 4715], '6.Citron': [718], 'M.': [719, 813, 882, 900, 1324, 1509, 2245, 2287, 2305, 2409, 2548, 3265, 3519, 3754, 3781, 3799, 4995, 5018, 5036, 5121, 5139, 5389, 5464, 5553, 5636, 5659, 5677, 5885], 'Teplow': [720, 778, 1289, 1360, 1474, 2210, 2374, 2513, 3230, 3484, 3719, 4960, 5354, 5429, 5518, 5601, 5850], 'D.B.': [721, 779, 954, 1290, 1361, 1475, 2211, 2375, 2514, 3231, 3485, 3720, 4961, 5355, 5430, 5519, 5602, 5851], 'Neuron.': [724], '1995;': [725], '14:': [726, 862], '661-670Abstract': [727], '(233)': [733], 'etc.,': [736], 'but': [737, 2429, 2661, 3086, 3184, 3439, 3568, 4114, 4430, 4455, 4798, 5309, 5725, 5797, 5816], 'until': [738], 'identity': [741], 'unknown.': [748], 'changed': [750], 'when': [751, 5738], 'very': [753], 'identified': [755, 2097, 4909], 'protease': [760, 1108, 4046, 5967], 'major': [764, 3216, 5232], '(7.Vassar': [766, 1277, 1462, 2198, 2362, 2501, 3218, 3472, 3707, 4948, 5342, 5417, 5506, 5589, 5838], 'R.': [767, 785, 874, 878, 934, 1278, 1296, 1463, 1481, 2199, 2217, 2279, 2283, 2363, 2381, 2502, 2520, 3219, 3237, 3473, 3491, 3708, 3726, 3773, 3777, 4949, 4967, 5010, 5014, 5113, 5117, 5343, 5361, 5418, 5436, 5507, 5525, 5590, 5608, 5651, 5655, 5839, 5857], 'Bennett': [768, 1279, 1464, 2200, 2364, 2503, 3220, 3474, 3709, 4950, 5344, 5419, 5508, 5591, 5840], 'B.D.': [769, 1280, 1465, 2201, 2365, 2504, 3221, 3475, 3710, 4951, 5345, 5420, 5509, 5592, 5841], 'Babu-Khan': [770, 1281, 1466, 2202, 2366, 2505, 3222, 3476, 3711, 4952, 5346, 5421, 5510, 5593, 5842], 'S.': [771, 773, 781, 789, 793, 870, 914, 1282, 1284, 1292, 1300, 1304, 1467, 1469, 1477, 1485, 1489, 2203, 2205, 2213, 2221, 2225, 2275, 2319, 2367, 2369, 2377, 2385, 2389, 2506, 2508, 2516, 2524, 2528, 3223, 3225, 3233, 3241, 3245, 3477, 3479, 3487, 3495, 3499, 3712, 3714, 3722, 3730, 3734, 3769, 3813, 4953, 4955, 4963, 4971, 4975, 5006, 5050, 5109, 5153, 5347, 5349, 5357, 5365, 5369, 5422, 5424, 5432, 5440, 5444, 5511, 5513, 5521, 5529, 5533, 5594, 5596, 5604, 5612, 5616, 5647, 5691, 5843, 5845, 5853, 5861, 5865], 'Kahn': [772, 1283, 1468, 2204, 2368, 2507, 3224, 3478, 3713, 4954, 5348, 5423, 5512, 5595, 5844], 'Mendiaz': [774, 1285, 1470, 2206, 2370, 2509, 3226, 3480, 3715, 4956, 5350, 5425, 5514, 5597, 5846], 'E.A.': [775, 1286, 1471, 2207, 2371, 2510, 3227, 3481, 3716, 4957, 5351, 5426, 5515, 5598, 5847], 'Denis': [776, 1287, 1472, 2208, 2372, 2511, 3228, 3482, 3717, 4958, 5352, 5427, 5516, 5599, 5848], 'P.': [777, 783, 894, 910, 1288, 1294, 1473, 1479, 2209, 2215, 2299, 2315, 2373, 2379, 2512, 2518, 3229, 3235, 3483, 3489, 3718, 3724, 3793, 3809, 4959, 4965, 5030, 5046, 5133, 5149, 5353, 5359, 5428, 5434, 5517, 5523, 5600, 5606, 5671, 5687, 5849, 5855], 'Ross': [780, 1291, 1476, 2212, 2376, 2515, 3232, 3486, 3721, 4962, 5356, 5431, 5520, 5603, 5852], 'Amarante': [782, 1293, 1478, 2214, 2378, 2517, 3234, 3488, 3723, 4964, 5358, 5433, 5522, 5605, 5854], 'Loeloff': [784, 1295, 1480, 2216, 2380, 2519, 3236, 3490, 3725, 4966, 5360, 5435, 5524, 5607, 5856], 'Luo': [786, 1297, 1482, 2218, 2382, 2521, 3238, 3492, 3727, 4968, 5362, 5437, 5526, 5609, 5858], 'Y.': [787, 1298, 1483, 2219, 2383, 2522, 3239, 3493, 3728, 4969, 5363, 5438, 5527, 5610, 5859], 'Fisher': [788, 1299, 1484, 2220, 2384, 2523, 3240, 3494, 3729, 4970, 5364, 5439, 5528, 5611, 5860], 'Fuller': [790, 1301, 1486, 2222, 2386, 2525, 3242, 3496, 3731, 4972, 5366, 5441, 5530, 5613, 5862], 'Edenson': [792, 1303, 1488, 2224, 2388, 2527, 3244, 3498, 3733, 4974, 5368, 5443, 5532, 5615, 5864], 'Lile': [794, 1305, 1490, 2226, 2390, 2529, 3246, 3500, 3735, 4976, 5370, 5445, 5534, 5617, 5866], 'Jarosinski': [796, 1307, 1492, 2228, 2392, 2531, 3248, 3502, 3737, 4978, 5372, 5447, 5536, 5619, 5868], 'M.A.': [797, 1308, 1493, 2229, 2393, 2532, 3249, 3503, 3738, 4979, 5373, 5448, 5537, 5620, 5869], 'Biere': [798, 1309, 1494, 2230, 2394, 2533, 3250, 3504, 3739, 4980, 5374, 5449, 5538, 5621, 5870], 'A.L.': [799, 1310, 1495, 2231, 2395, 2534, 3251, 3505, 3740, 4981, 5375, 5450, 5539, 5622, 5871], 'Curran': [800, 1311, 1496, 2232, 2396, 2535, 3252, 3506, 3741, 4982, 5376, 5451, 5540, 5623, 5872], 'E.': [801, 1312, 1497, 2233, 2397, 2536, 3253, 3507, 3742, 4983, 5377, 5452, 5541, 5624, 5873], 'Burgess': [802, 1313, 1498, 2234, 2398, 2537, 3254, 3508, 3743, 4984, 5378, 5453, 5542, 5625, 5874], 'T.': [803, 1314, 1499, 2235, 2399, 2538, 3255, 3509, 3744, 4985, 5379, 5454, 5543, 5626, 5875], 'Louis': [804, 1315, 1500, 2236, 2400, 2539, 3256, 3510, 3745, 4986, 5380, 5455, 5544, 5627, 5876], 'J.-C.': [805, 1316, 1501, 2237, 2401, 2540, 3257, 3511, 3746, 4987, 5381, 5456, 5545, 5628, 5877], 'Collins': [806, 1317, 1502, 2238, 2402, 2541, 3258, 3512, 3747, 4988, 5382, 5457, 5546, 5629, 5878], 'F.': [807, 1318, 1503, 2239, 2403, 2542, 2726, 3259, 3513, 3748, 4989, 5383, 5458, 5547, 5630, 5879], 'Treanor': [808, 1319, 1504, 2240, 2404, 2543, 3260, 3514, 3749, 4990, 5384, 5459, 5548, 5631, 5880], 'Rogers': [810, 1321, 1506, 2242, 2406, 2545, 3262, 3516, 3751, 4992, 5386, 5461, 5550, 5633, 5882], 'Citron': [812, 1323, 1508, 2244, 2408, 2547, 3264, 3518, 3753, 4994, 5388, 5463, 5552, 5635, 5884], 'Science.': [814, 1325, 1510, 2246, 2410, 2549, 3266, 3520, 3755, 4996, 5390, 5465, 5554, 5637, 5886], '1999;': [815, 861, 925, 964, 1326, 1511, 2247, 2330, 2411, 2550, 3267, 3521, 3756, 3824, 4997, 5061, 5164, 5391, 5466, 5555, 5638, 5702, 5887, 5982], '286:': [816, 1327, 1512, 2248, 2412, 2551, 3268, 3522, 3757, 4998, 5392, 5467, 5556, 5639, 5888], '735-741Crossref': [817, 1328, 1513, 2249, 2413, 2552, 3269, 3523, 3758, 4999, 5393, 5468, 5557, 5640, 5889], '(3307)': [820, 1331, 1516, 2252, 2416, 2555, 3272, 3526, 3761, 5002, 5396, 5471, 5560, 5643, 5892], 'Three': [823], 'subsequently': [824, 3409], 'published': [825], 'independent': [826], '(8.Hussain': [828], 'I.': [829, 898, 1355, 2303, 3797, 4071, 5034, 5137, 5675], 'Powell': [830], 'D.': [831, 880, 908, 916, 1359, 2285, 2313, 2321, 3779, 3807, 3815, 5016, 5044, 5052, 5119, 5147, 5155, 5657, 5685, 5693], 'Howlett': [832], 'D.R.': [833, 5318, 5925], 'Tew': [834], 'D.G.': [835], 'Meek': [836], 'T.D.': [837], 'Chapman': [838], 'Gloger': [840], 'I.S.': [841], 'Murphy': [842], 'K.E.': [843], 'Southan': [844], 'C.D.': [845], 'Ryan': [846], 'D.M.': [847], 'Smith': [848], 'T.S.': [849], 'Simmons': [850], 'D.L.': [851], 'Walsh': [852], 'F.S.': [853], 'Dingwall': [854], 'Christie': [856], 'Mol.': [858], 'Neurosci.': [860], '419-427Crossref': [863], '(1001)': [866], 'Scholar,': [868, 932, 5004, 5645], '9.Sinha': [869, 5005, 5646], 'Anderson': [871, 2276, 3770, 5007, 5110, 5648], 'J.P.': [872, 2277, 3771, 5008, 5111, 5649], 'Barbour': [873, 2278, 3772, 5009, 5112, 5650], 'Basi': [875, 2280, 3774, 5011, 5114, 5652], 'G.S.': [876, 2281, 3775, 5012, 5115, 5653], 'Caccavello': [877, 2282, 3776, 5013, 5116, 5654], 'Davis': [879, 2284, 3778, 5015, 5118, 5656], 'Doan': [881, 2286, 3780, 5017, 5120, 5658], 'Dovey': [883, 2288, 3782, 5019, 5122, 5660], 'H.F.': [884, 2289, 3783, 5020, 5123, 5661], 'Frigon': [885, 2290, 3784, 5021, 5124, 5662], 'N.': [886, 892, 2291, 2297, 3785, 3791, 5022, 5028, 5125, 5131, 5663, 5669], 'Hong': [887, 2292, 3786, 5023, 5126, 5664], 'Jacobson-Croak': [889, 2294, 3788, 5025, 5128, 5666], 'Jewett': [891, 2296, 3790, 5027, 5130, 5668], 'Keim': [893, 2298, 3792, 5029, 5132, 5670], 'Knops': [895, 2300, 3794, 5031, 5134, 5672], 'Lieberburg': [897, 1354, 2302, 3796, 5033, 5136, 5674], 'Power': [899, 2304, 3798, 5035, 5138, 5676], 'Tan': [901, 2306, 3800, 5037, 5140, 5678], 'H.': [902, 940, 2307, 3801, 5038, 5141, 5679], 'Tatsuno': [903, 2308, 3802, 5039, 5142, 5680], 'Tung': [905, 2310, 3804, 5041, 5144, 5682], 'Schenk': [907, 1358, 2312, 3806, 5043, 5146, 5684], 'Seubert': [909, 2314, 3808, 5045, 5148, 5686], 'Suomensaari': [911, 2316, 3810, 5047, 5150, 5688], 'S.M.': [912, 2317, 3811, 5048, 5151, 5689], 'Wang': [913, 2318, 3812, 5049, 5152, 5690], 'Walker': [915, 2320, 3814, 5051, 5154, 5692], 'Zhao': [917, 2322, 3816, 5053, 5156, 5694], 'Mc': [919, 2324, 3818, 5055, 5158, 5696], 'Conlogue': [920, 2325, 3819, 5056, 5159, 5697], 'L.': [921, 2326, 3820, 5057, 5160, 5698], 'Varghese': [922, 2327, 3821, 5058, 5161, 5699], '402:': [926, 965, 2331, 3825, 5062, 5165, 5703], '537-540Crossref': [927, 2332, 3826, 5063, 5166, 5704], '(1482)': [930, 2335, 3829, 5066, 5169, 5707], '10.Yan': [933], 'Bienkowski': [935], 'M.J.': [936], 'Shuck': [937], 'M.E.': [938, 962], 'Miao': [939], 'Tory': [941], 'M.C.': [942], 'Pauley': [943], 'A.M.': [944], 'Brashier': [945], 'J.R.': [946], 'Stratman': [947], 'N.C.': [948], 'Mathews': [949], 'W.R.': [950], 'Buhl': [951], 'A.E.': [952], 'Carter': [953], 'Tomasselli': [955], 'A.G.': [956], 'Parodi': [957], 'L.A.': [958], 'Heinrikson': [959], 'R.L.': [960], 'Gurney': [961], '533-537Crossref': [966], '(1339)': [969], 'confirmed': [973, 2419, 3052, 4124, 4240, 4938], 'this': [974, 1557, 2601, 2887, 3195, 4241, 4744, 5094, 5216, 5492, 5573, 5736, 5760, 6029], 'conclusion.': [975], 'characterize': [978, 3150], 'protein.': [981, 5210], 'show': [983, 2600, 2652, 3073, 3102, 4513, 4547, 4839, 4878, 5258, 5942], 'N-glycosylated': [988, 2880, 2926, 2933], 'determine': [1002, 1193, 4176, 4296, 4498, 4531], 'N-glycosylation': [1006, 4217, 4618, 4654, 4682], 'bonds.': [1011, 3356, 4003, 5802], 'Our': [1012, 5256], 'results': [1013, 3015, 4066, 4139, 5257], 'member': [1020, 5833], 'family.': [1024], 'Trypsin,': [1025], 'pepsin,': [1026, 5910], 'endoproteinase': [1028, 1688, 3956, 4020, 4048], 'were': [1030, 1059, 1087, 1095, 1113, 1121, 1166, 1205, 1268, 1384, 1421, 1618, 1655, 1699, 1762, 1784, 1801, 1853, 1893, 1907, 1912, 1983, 2031, 2096, 2117, 2150, 2172, 2837, 2852, 3281, 3626, 3856, 4104, 4424, 4449, 4493, 4526, 4674, 4826, 4865], 'obtained': [1031, 1052, 2633, 3410], 'Roche': [1033, 1068], 'Molecular': [1034, 1042, 1069], 'Biochemicals.': [1035, 1070], 'Fluorescein': [1036], '(FM)': [1038], 'purchased': [1040, 1066], 'Probes': [1043], '(Eugene,': [1044], 'OR).': [1045], '4-HCCA': [1046, 1978, 4594], 'Sigma.': [1049], 'Sialidase': [1050], 'Glyko': [1054], '(Novato,': [1055], 'CA).': [1056, 2060], 'N-and': [1057], 'O-glycanases': [1058], 'Genzyme': [1061], '(Cambridge,': [1062], 'MA).N-Glycosidase': [1063], 'F': [1064, 1374, 2612, 2681], 'Other': [1071], 'chemicals': [1072], 'quality': [1076], 'grade.': [1077], 'Untransfected': [1078], '293': [1079, 1082, 2343, 2569, 2692, 2710, 2718, 2733, 2758, 2949, 3366, 5077, 5176], 'or': [1081, 1199, 1212, 1758, 1851, 1951, 1977, 2145, 2446, 2702, 2803, 3629, 3764, 4032, 4047, 5229, 5767, 5913], 'stably': [1084, 1527, 2345, 2712, 2720, 2731, 2756], 'scraped': [1088], 'into': [1089, 2458, 2940, 4697], 'phosphate-buffered': [1090, 1578], 'saline,': [1091], 'precipitated.': [1096], 'pellet': [1098, 1158], 'resuspended': [1100], '25': [1102, 1169], 'mm': [1103, 1170, 1214, 1403, 1599, 1871, 1900], 'HEPES,': [1104, 1171], 'pH': [1105, 1172, 1216, 1608, 1673, 1727, 1875, 1904, 2451], '7.2,': [1106, 1173], 'inhibitors,': [1109], 'swollen': [1114], 'on': [1115, 1429, 1970, 2046, 2101, 2479, 3383, 4677], 'ice': [1116], '60': [1118, 1148, 3012], 'min.': [1119, 1184, 1831], 'Cells': [1120, 3062], 'lysed': [1122], '3': [1124, 2797, 2885, 3065, 3100, 3443, 3932, 4440, 4465], 'freeze-thaw': [1125], 'cycles': [1126, 3842], '−80': [1128], '°C': [1129, 1664, 1736], 'then': [1131, 1391, 1968, 2843], 'centrifuged': [1132, 1146, 1177], '15': [1134], 'min': [1135, 1149, 1389, 1817, 2564, 2696, 2737, 2841], '1,000': [1137], '×': [1138, 1152, 1180, 1574, 1632, 1712, 1774], 'g': [1139, 1181], 'precipitate': [1141], 'nuclei.': [1142], 'supernatant': [1144, 1161, 1187], '100,000': [1151], 'gto': [1153], 'give': [1154], 'crude': [1156, 1203], 'containing': [1162, 1440, 4224, 4348, 4693, 5474], 'cytosolic': [1163], 'proteins.': [1164, 1191, 1222], 'Membranes': [1165], 'solubilized': [1167], '2%': [1174, 1808], 'CHAPS': [1175], '20,000': [1179], '10': [1183, 1598, 1830, 3912], 'resulting': [1186], 'contained': [1188, 4232, 4847, 4886], 'membrane-bound': [1190, 5741], 'To': [1192, 2817, 4004, 5399], 'if': [1194, 5334], 'peripheral': [1200], 'membranes': [1204], 'washed': [1206], 'either': [1208, 1945], '0.5': [1209, 2022, 2442], 'm': [1210, 1605, 1670, 2443, 2448], 'NaCl': [1211], '100': [1213], 'Na2CO3,': [1215], '11,': [1217, 2452], 'peripherally': [1220], 'bound': [1221], 'A': [1223, 1533, 1537, 1781, 1787, 3056, 3072, 3098, 3125, 3376, 5282], 'polyclonal': [1224], 'antibody': [1225, 2637, 2649, 2863, 2959, 3085], 'specific': [1226], 'propeptide': [1229, 1336, 2641, 2704, 2773, 2783, 2958, 3090, 3116, 3140, 5091, 5277, 5292, 5302, 5746], 'region': [1230, 2642], 'raised': [1234, 2638], 'following': [1235, 1338], 'standard': [1236, 2029], 'procedures': [1237], 'using': [1238, 1270, 1565, 1626, 1706, 1768, 1944, 1986, 1990, 2033, 2142, 2698, 2747, 2771, 3663, 3945, 4583, 5572], 'immunogen': [1240], 'peptide': [1242, 1644, 1840, 2131, 3891, 3959, 4035, 4051, 4149, 4196, 4271, 4292, 4329, 4346, 4360, 4387, 4395, 4691, 4745, 4845, 4884, 5722], 'CGIRLPLRSGLGGAPLGLRLPR': [1243], '(comprising': [1244], 'acids': [1246], '25–45': [1247], 'residue': [1254, 4087, 4260, 4300], 'coupling).': [1256], 'After': [1257, 4641], 'metabolic': [1258], 'labeling': [1259, 2565, 2697, 2738, 2762, 2872], '[35S]methionine': [1261], 'aliquots': [1262], 'same': [1265, 1694, 1920, 2596, 2630, 2664, 2673, 2967, 4670], 'lysates': [1267, 2341, 2851], 'immunoprecipitated': [1269, 2561, 2859], 'previously': [1272, 1461, 3173, 3471, 5413, 5583], 'described': [1273, 1340, 1460, 3174, 3470, 3669, 5414, 5584], 'C-terminal': [1275, 2360, 2573, 2669, 2700, 2749, 2787, 2862, 2975, 3084, 3113, 6001], 'antiserum': [1276, 1337, 2361, 2701], 'protocols': [1339], 'before': [1341, 5107], '(11.Haass': [1342], 'Schlossmacher': [1344], 'M.G.': [1345], 'Hung': [1346], 'A.Y.': [1347], 'Vigo-Pelfrey': [1348], 'Mellon': [1350], 'Ostaszewski': [1352], 'B.L.': [1353], 'Koo': [1356], 'E.H.': [1357], '1992;': [1365], '359:': [1366], '322-325Crossref': [1367], '(1763)': [1370], 'N-Glycosidase': [1373], 'treatment': [1375, 2270, 3057, 3119, 3454, 3545, 5283, 6034], 'performed': [1377, 1723, 1943, 2045, 2076, 2139, 2829, 2953, 4026], 'after': [1378, 2129, 2269, 2562, 2694, 2735, 2760, 2871, 3452, 3544, 3600, 3886], 'immunoprecipitation.': [1379], 'For': [1380, 1858, 2061, 4688], 'pulse-chase': [1381, 2831, 2954], 'experiments': [1382, 2955, 5789], 'metabolically': [1385], 'labeled': [1386, 2838], '20': [1388, 1614, 1666, 1732, 1870, 1886, 1899, 2563, 2695, 2736, 2840], 'chased': [1392, 2795, 2844, 3063, 3095], 'indicated': [1395, 2742, 2766, 2856, 3965, 4129], 'times.': [1396], 'Brefeldin': [1397, 2807, 3055, 3071, 3097, 3124, 5281], 'A,': [1398, 2586, 2687, 4566], 'dissolved': [1399, 1868, 1960], '30': [1402, 1410, 3536], 'stock': [1404], 'methanol,': [1406], 'μm': [1411], 'final': [1412], 'concentration': [1413], 'medium': [1415], 'during': [1416], '3-h': [1418], 'chase.': [1419], 'Immunoprecipitates': [1420], 'SDS-PAGE': [1424, 3388, 3430, 3451, 3543], 'followed': [1425, 2739, 2763, 3379], 'quantitative': [1427], 'imaging': [1428], 'STORM': [1431], '860': [1432], 'phosphorimaging': [1433], 'system': [1434, 2053, 2070], '(Molecular': [1435], 'Dynamics).': [1436], 'BACE-IgG': [1438, 1519, 1541, 1652, 3297, 3389, 3457, 3463, 3596, 3646, 3849, 3935, 4014, 5415, 5578, 5749], 'construct': [1439, 5416], 'cDNA': [1441], 'encoding': [1442], '(residues': [1447, 3305], '1–460)': [1448, 3306], 'portion': [1452, 3001, 3310, 3325, 4381], 'human': [1454, 3315, 3363, 3766, 5916], 'IgG1': [1455], '(230': [1456, 3311], 'acids)': [1458, 3313], 'purified': [1522, 1561, 1925, 2115, 2173, 3189, 3369, 3456, 3572, 3643, 3848, 5483], 'conditioned': [1524, 3372], 'media': [1525, 3373], 'transfected': [1528, 2732, 2757, 3705], '293T': [1529], 'columns.': [1534], 'eluate': [1538], 'consisted': [1539], 'level': [1545, 5241, 6041], 'clipped': [1547], 'fragment.': [1549], 'order': [1551, 1582, 3148], 'remove': [1553], 'contaminant,': [1556], 'material': [1558, 2898, 3004, 3287, 5205], 'further': [1560, 2118, 3131], 'filtration': [1564, 3382], 'Sephacryl': [1567, 3384], 'S-300HR': [1568], '(Amersham': [1569], 'Pharmacia': [1570], 'Biotech)': [1571], 'column': [1572, 1630, 1710, 1772], '(3.2': [1573], '46': [1575], 'cm)': [1576], 'saline': [1579], 'buffer.': [1580], 'examine': [1584], 'existence': [1586], 'free': [1588, 3942, 5795], 'sulfhydryl': [1589, 3943], 'BACE-IgG,': [1592], 'sample': [1594, 1649, 1678, 1866, 1923, 1958, 1972, 3575, 3648, 4510, 4544, 4587], 'treated': [1596, 1854, 2594, 2723], 'FM': [1600, 3946], '50': [1602, 2621], 'mmTris-HCl,': [1603], '4': [1604], 'guanidine': [1606], 'HCl,': [1607, 1726], '7.5,': [1609], 'room': [1611], 'temperature': [1612], 'h.': [1615], 'Excess': [1616], 'reagents': [1617], 'removed': [1619], 'passing': [1621], 'through': [1622, 4851, 4890], 'reversed': [1623, 1703, 1753, 1765, 1927, 2175], 'phase': [1624, 1704, 1754, 1766, 1928, 2176, 2461], 'HPLC': [1625, 1705, 1767, 1929, 2177, 4678, 4713], 'Vydac': [1628, 1708, 1770], 'C18': [1629, 1709, 1771], '(2.1': [1631, 1711, 1773], '150': [1633, 1713, 1775], 'mm).': [1634, 1714, 1776], 'fraction': [1637, 2353, 2434, 2440], 'subjected': [1639, 1701, 1908], 'digestion': [1642, 1686, 1716, 1746, 2144, 4024, 4417], 'mapping.': [1645], 'above': [1647, 1916], 'FM-modified': [1648], '(∼50': [1653, 1720], 'μg)': [1654, 1661, 1691, 1721], 'initially': [1656], 'digested': [1657, 1697, 3952, 4016, 4188], 'trypsin': [1659, 3954, 4018], '(1': [1660, 1690], '37': [1663, 1735, 1889], 'h': [1667, 1733, 1887, 2798, 2886, 2980, 3066, 3101], '0.1': [1669, 2447], 'Tris': [1671], 'buffer,': [1672, 1874, 1903], '7.5': [1674], '(200': [1675, 1729], 'μl).': [1676], 'allowed': [1680], 'proceed': [1682], 'second': [1685, 1819, 2636], 'under': [1692, 1918, 3670, 4191, 5100, 5239, 5253, 5307], 'conditions.': [1695, 1921, 4193, 5255], 'materials': [1698], 'directly': [1700, 4006], 'Peptic': [1715], '0.02n': [1725], '2': [1728, 2585, 2646, 2865, 2916, 2961, 2979, 3060, 3593], 'μl),': [1730, 1878], 'enzyme:substrate': [1739], 'ratio': [1740, 3915], '1:50': [1742], '(w/w),': [1743], 'terminated': [1748], 'direct': [1750], 'injection': [1751], 'onto': [1752, 3578, 3651, 4590], 'HPLC.': [1755], 'Trypsin-endoproteinase-Asp-N': [1756], '(TD)-': [1757], '(P)-generated': [1760], 'peptides': [1761, 1800, 1941, 1982, 2041, 4031, 4057, 4103, 4123, 4183, 4211, 4225, 4315, 4412, 4478, 4489, 4522, 4849, 4888], 'separated': [1763, 2349, 4696], 'Two': [1777, 3854, 4520], 'solvent': [1778, 1786, 1792, 1809, 1813, 1823, 1827, 1998], '(solvents': [1780], 'B)': [1783], 'utilized,': [1785], '(0.1%': [1788, 1794], 'trifluoroacetic': [1789, 1795, 1963, 2004], 'acid)': [1790], 'B': [1793, 1810, 1814, 1824, 1828], 'acid,': [1796, 1862, 1964], '90%': [1797], 'acetonitrile).': [1798], 'eluted': [1802, 4676], 'linear': [1805], 'gradient': [1806, 1820], '40%': [1812, 1822], 'over': [1815, 1829], '40': [1816], '60%': [1826], 'Flow': [1832], 'constant': [1835], '0.25': [1837], 'ml/min.': [1838], 'detected': [1842, 2583, 2971, 4304, 4426, 4451], 'absorbances': [1844], '215': [1846], '280': [1848], 'nm.': [1849], 'Glycoprotein': [1850], 'glycopeptides': [1852, 2116, 4609, 4667, 4775], 'several': [1856, 4056, 4698, 4718], 'enzymes.': [1857, 6012], 'removal': [1859], 'sialic': [1861], 'dried': [1864], 'sodium': [1872, 1901, 2155, 2444, 2449], 'acetate': [1873, 1902], '5': [1876, 2008, 3595], '(50': [1877], 'incubated': [1880, 1913], 'sialidase': [1882, 3553, 3557, 3616], '(0.1': [1883], 'unit)': [1884], '°C.': [1890], 'Protein': [1891, 3659], 'samples': [1892, 2716], 'deglycosylated': [1894], 'N-': [1896], 'andO-glycanases': [1897], '5,': [1905], 'SDS-PAGE.': [1910], 'Glycopeptides': [1911], 'enzymes': [1917], 'mass': [1931, 1953, 2016, 2026, 2125, 2181, 2487, 3009, 3407, 3421, 3434, 3660, 3666, 3674, 3685, 4206, 4246, 4356, 4367, 4443, 4484, 4516, 4550, 4567, 4572, 4577, 4708, 4719, 4753, 4762, 4770, 4800, 4855], 'spectrometry.': [1932, 2182], 'Matrix-assisted': [1933], '(MALDI)-mass': [1937], 'spectrometry': [1938, 3640, 4097, 4120, 4207, 4239, 4326, 4562, 4709], 'Kratos': [1947, 4584], 'IV': [1948], '(Kratos': [1949], 'Analytical)': [1950], 'Voyager': [1952, 3665], 'spectrometer': [1954, 3667, 4582], '(PerSeptive': [1955], 'Biosystems).': [1956], '0.1%': [1962, 2003], '50%': [1965, 2000], 'acetonitrile': [1966], 'spotted': [1969], 'plate': [1973], 'sinapinic': [1975, 3657], 'matrix.': [1980], 'Cys-containing': [1981, 4030, 4411, 4564], 'also': [1984, 2952, 5711], 'ion-spray': [1988], 'interface': [1989], 'MichromeBIOSOURCE': [1992], 'Ultrafast': [1993], 'Microprotein': [1994], 'Analyzer.': [1995], 'carrier': [1997], 'acetonitrile:water': [2001], 'flowing': [2006], 'μl/min.': [2009], 'scan': [2011], 'range': [2012], '300–2400': [2014], 'atomic': [2015, 2023, 6040], 'units': [2017, 2027], 'step': [2020], 'unit.': [2024], 'deviation': [2030], 'calculated': [2032], 'Sciex': [2034], 'hypermass': [2035], 'software.': [2036], 'N-Terminal': [2037], 'sequence': [2038, 2112, 2188, 3844, 3902, 4093, 4117, 4306, 4428, 4437, 4453, 4462, 4642, 4673, 4730, 4737, 5338, 5502], 'proteins': [2043], 'model': [2048, 2081], '494': [2049], 'ABI': [2050, 2068], 'Procise': [2051], 'sequencer': [2052], 'Perkin-Elmer/Applied': [2055], 'Biosystems': [2056, 2080], 'Inc.': [2057], '(Foster': [2058], 'City,': [2059], 'PTH': [2064, 2102], 'acids,': [2066], '140': [2069], 'used.': [2072], 'Data': [2073], 'Applied': [2079], '610': [2082], 'data': [2083, 4763], 'program': [2085], 'sequencing,': [2088], 'version': [2089], '2.1.': [2090], 'N-Glycosylation': [2091, 4863], 'negative': [2099], 'response': [2100], 'corresponding': [2106, 3870, 3908, 4835, 4874], 'Asn': [2107, 4298], 'cycle': [2108], 'consensus': [2111, 4638, 4840, 4879], 'NX(S/T).': [2113, 4640], 'MALDI-mass': [2121, 3412, 4581], 'spectrometry,': [2122, 3413], 'indicating': [2123, 2623, 3138], 'carbohydrate': [2127, 2136, 3038, 4323, 4614, 4662, 4686, 4766, 4792], 'moiety': [2128], 'subtracting': [2130], 'mass.': [2132], 'Another': [2133, 4376], 'strategy': [2134], 'deglycosylation': [2141], 'N-glycanase': [2143, 3547], 'hydrazinolysis.': [2146], 'sugar': [2148], 'components': [2149, 3918], 'derivatized': [2151], '2-aminobenzamide': [2153], 'borohydride': [2156], '(12.Anumula': [2157], 'K.R.': [2158], 'Dhume': [2159], 'S.T.': [2160], 'Glycobiology.': [2161], '8:': [2163], '685-694Crossref': [2164], '(242)': [2167], 'derivatives': [2171], 'Analysis': [2183, 5069], 'suggests': [2189, 3435, 3991, 4679, 4803], 'single': [2194, 3392, 3416, 4712], 'can': [2263, 3185, 5481], 'fractions': [2268], '0.2%': [2272], 'Triton': [2273], '(9.Sinha': [2274, 3768, 5108], 'prepared': [2339, 2853], 'overexpressing': [2346, 5076], 'cytoplasm': [2350], 'ultracentrifugation.': [2355], 'Immunoprecipitation': [2356], 'present': [2423], 'only': [2424, 3074, 3276, 5962], '(M),': [2428], 'not': [2430, 2454, 2599, 2651, 3087, 3212, 3440, 3963, 4038, 4171, 4303, 4331, 4425, 4450, 4497, 4512, 4530, 4546, 5184, 5213, 5751, 5776, 5792, 5807], 'cytoplasmic': [2433], '(C)': [2435], '(Fig.1).': [2436], 'Washing': [2437], 'chloride': [2445], 'carbonate,': [2450], 'does': [2453, 2650, 5183, 5791], 'wash': [2460], '(P),': [2462], 'demonstrating': [2463], 'indeed': [2467, 4659], '(I).': [2472], 'As': [2473, 2589, 5958], 'noted': [2474], 'before,': [2475], 'mature': [2476, 2902, 2942, 3076, 3897, 5087, 5209], 'migrates': [2478], 'gels': [2480], '∼70': [2482], 'kDa,': [2483, 2622, 3468], 'higher': [2485, 3010, 4249], 'molecular': [2486, 2674, 3008, 3420, 3433, 3590], 'than': [2488, 2620, 2893, 3011, 3190, 4250, 5200, 5487], 'predicted': [2489, 4366, 4634, 4806, 4933], 'sequence,': [2494, 4639], 'suggesting': [2495, 3837, 5743], 'glycosylated': [2500, 2903, 2943, 5271], 'When': [2558], 'stable': [2568, 2825, 2947, 5251], 'line': [2570, 2994], 'antibody,': [2574, 2670], 'immature': [2576, 2626, 2879, 2925, 2932, 3042, 3104, 3135, 5191, 5223, 5235], 'species': [2577, 2627, 2666, 2881, 5714], 'running': [2578, 3465, 3533], '∼60': [2580], 'kDa': [2581, 3397, 3424, 3537, 3677, 3688], '(Fig.': [2584, 2645, 2864, 2960, 2989, 3059, 3398, 3425, 3538, 4261, 4373, 4705], 'lane': [2587, 3400, 3540, 3598, 3604, 3607, 3610, 3614, 3621], 'B).': [2588, 2866, 3444, 3933, 4375], 'expected,': [2590], 'nontransfected': [2591, 2706], 'control': [2592, 2657, 2707], 'way': [2597, 3217], 'do': [2598, 5212], 'band': [2602, 2616, 2654, 2876, 2888, 2969, 3393], '(lane': [2603, 2613, 2659, 3548, 3551, 3554, 3560], 'C).': [2604, 2962], 'If': [2605], 'immunoprecipitate': [2607], 'pretreated': [2609], 'N-glycosidase': [2611, 2680, 2725], 'BF),': [2614], 'runs': [2617], 'less': [2619, 2892, 5199], 'isN-glycosylated.': [2628], 'result': [2631, 3990], 'A).': [2647, 4095, 4263, 4466], 'non-transfected': [2656], 'C)': [2660], 'recognizes': [2662], '60-kDaN-glycosylated': [2665], 'weight': [2675, 3591], 'shift': [2676], 'observed': [2678, 4245, 4355, 4816, 5731], 'upon': [2679], 'treatment.Figure': [2682], '2BACE': [2683], 'glycosylation.': [2686, 4335], 'immunoprecipitation': [2688, 2752, 2790], 'antiserum.C,': [2705], 'cells;': [2708], 'B,': [2709, 4571], 'BACE;': [2714], 'BF,': [2715], 'B,immunoprecipitation': [2727], 'chase': [2743, 2767, 2981], 'times': [2744, 2768, 2857], 'hours': [2746, 2770], 'antiserum.': [2750], 'C,': [2751], 'brief': [2761], 'antiserum.D,': [2774], 'quantitation': [2775, 2991], 'signal': [2779, 2986, 3890, 5721], 'phosphorimaging.Solid': [2781], 'line,': [2782, 2786, 2827], 'signal;': [2784], 'broken': [2785], 'signal.': [2788], 'E,': [2789, 5914], '(+)': [2802], 'absence': [2804, 2847, 3069], '(−)': [2805], 'A.View': [2808], 'Large': [2809, 3631, 3693, 4597], 'Image': [2810, 3632, 3694, 4598], 'Figure': [2811, 3633, 3695, 4560, 4599], 'ViewerDownload': [2812, 3634, 3696, 4600], 'Hi-res': [2813, 3635, 3697, 4601], 'image': [2814, 3636, 3698, 4602], 'Download': [2815, 3637, 3699, 4603], '(PPT)': [2816, 3700, 4604], 'analyze': [2818, 4005, 5400], 'turnover': [2820, 5072], 'experiment': [2832, 3058], 'label.': [2849], 'Cell': [2850], 'At': [2867, 2884, 2963, 5172], 'time': [2868, 2964, 2982], '0': [2869, 2965], 'immediately': [2870], 'strong': [2874], '60-kDa': [2875, 2968, 3105, 3136], 'representing': [2877], 'detectable.': [2883], 'disappeared,': [2890], 'half': [2894, 5201], 'original': [2897], 'recovered': [2900, 5207], '70-kDa': [2904, 3077], 'degraded': [2908, 5238], 'slowly': [2909], '(broken': [2910], 'line,T': [2911], '12': [2912], '>9': [2913], 'h,': [2914], 'Fig.': [2915, 3442, 3931, 4201, 4439, 4464], 'D).': [2917, 2997], 'Thus,': [2918, 3118, 3977, 4756], 'overexpressed': [2919], 'glycosylated,': [2922], 'rapidly': [2929, 5195], 'degraded.': [2930], 'escapes': [2936], 'degradation': [2937], 'turned': [2939, 5196], 'cells.': [2950], 'antibody;': [2976], 'however,': [2977], 'disappeared': [2988], '2C,': [2990], 'solid': [2993], 'Fig.2': [2996], 'Only': [2998], 'minor': [3000], 'kDa.': [3013], 'These': [3014, 6003], 'indicate': [3016, 5286], 'occurs': [3030], 'temporal': [3032], 'proximity': [3033], 'trimming/adding': [3036], 'form,': [3043, 3137], 'i.e.': [3044], 'finding': [3050], 'E).': [3061], 'detectable': [3081, 3109], 'antibody.': [3091, 3117], 'contrast,': [3093], 'blocks': [3126, 5284], 'glycosylation': [3132, 4012], 'happens': [3142, 5293], 'modifications': [3153], 'detail,': [3157, 5407], 'necessary': [3160], 'purify': [3162], 'quantity': [3165], 'homogeneity.': [3170], 'soluble': [3176, 5574], 'retains': [3181], 'enzymatic': [3182, 3199], 'activity': [3183], 'more': [3187, 5406, 5485], 'easily': [3188], 'form.': [3193, 5490, 5575], 'Because': [3194, 3322, 5491], 'fusion': [3196, 3279, 3292, 3328, 3358, 3464], 'shows': [3198, 3460, 3562], 'activity,': [3200], 'compromised': [3213], 'able': [3283], 'get': [3285], 'sufficient': [3286, 4173], 'biochemical': [3289, 4915], 'characterization.': [3290], 'termed': [3296], 'extracellular': [3301], 'γ-immunoglobulin': [3316], 'Fig.3': [3320], 'IgG': [3324, 3349, 3531, 3906, 4632], 'forms': [3330, 5773, 5810], 'homodimeric': [3332], 'piece,': [3334], 'expected': [3336, 3927, 4252, 4515, 4549], 'find': [3338], '(BACE)2-(IgG)2': [3344], 'molecules': [3350], 'connected': [3352], 'intermolecular': [3354, 4399, 5814], 'embryonic': [3364], 'kidney': [3365], 'affinity': [3377], 'chromatography,': [3378], 'S-300-HR.': [3385], 'On': [3386], 'non-reducing': [3387], 'showed': [3390, 3850, 4111, 4279, 5990], 'approximately': [3395, 3535], '116': [3396, 3423, 3676], '4,': [3399, 3539, 3587], '2).': [3401], 'An': [3402], 'exact': [3403, 4791], 'measure': [3404], 'revealing': [3414], 'component': [3417], '5),': [3426], 'consistent': [3427, 4363], 'result.': [3431], 'BACE-(IgG)2': [3438, 3929], '(BACE)2-(IgG)2(see': [3441], 'Consistent': [3445], 'proposed': [3448], 'BACE-(IgG)2,': [3450], 'reducing': [3453], 'β-mercaptoethanol': [3459], 'monomeric': [3462], '90': [3467], 'piece': [3532], '3).': [3541], 'Nonreducing': [3542], '6),': [3549], 'O-glycanase': [3550, 3559, 3628], '8),': [3552], '9),': [3555], '+': [3558], '10)': [3561], 'multipleN-glycosylation': [3566], 'insignificantO-glycosylation.Figure': [3569], '4SDS-PAGE': [3570], 'BACE-IgG.': [3573], 'loaded': [3577, 3650, 4589], 'nonreducing': [3580], '(4–20%)': [3582], 'SDS': [3584], 'buffer.Lanes': [3585], '1,': [3586], '7,': [3589], 'markers;lanes': [3592], 'untreated;': [3597], '3,': [3599], 'reduction': [3601], 'β-mercaptoethanol;': [3603], '6,N-glycanase-treated': [3605], 'sample;': [3606, 3609, 3613], '8,O-glycanase-treated': [3608], '9,': [3611], 'sialidase-treated': [3612], '10,': [3615], '+O-glycanase-treated': [3617], 'sample.': [3618], 'Bands': [3619], '8–10around': [3622], '55-kDa': [3624], 'marker': [3625], 'sialidase.View': [3630], '(PPT)Figure': [3638], '5MALDI-mass': [3639], 'slide': [3653, 4592], 'matrix': [3656], 'acid.': [3658], '"Experimental': [3671], 'Procedures."': [3672], 'represents': [3678], 'singly': [3680], 'charged': [3681, 3691], 'ion': [3682], '58': [3687], 'doubly': [3690], 'ion.View': [3692], 'Full-length': [3701], 'isolated': [3703], 'predominantly': [3833], 'position': [3835], '46,': [3836, 3869], 'efficient': [3838, 5754], 'proprotein': [3839], 'processing.': [3840], 'Ten': [3841], 'multiple': [3851, 4765], 'sequences.': [3853, 3976], 'sequences': [3855, 3872, 4234, 4841, 4880], 'derived': [3857, 3984, 4433, 4458], 'starting': [3864, 5715], '22': [3867], 'TQHGIRLPLR-(22–31)': [3873], 'ETDEEPEEPG-(46–55).': [3875], 'interpret': [3877], '22-form': [3879], 'pro-form': [3882], '46-form': [3894], 'species.': [3899], 'third': [3901, 4337], 'comes': [3903], 'portion,': [3907], 'AVTDKTHTXP-(461–470)': [3910], 'residues.': [3913], 'roughly': [3920], '1:1': [3921], 'IgG,': [3925], 'Purified': [3934], 'examined': [3937], 'labeling.': [3947], 'FM-labeled': [3949], 'Asp-N.': [3957], 'mapping': [3960], '(data': [3962, 4037], 'shown)': [3964, 4039], 'few': [3967], 'fluorescent-positive': [3968], 'peaks.': [3969], 'However,': [3970, 5939], 'none': [3971], 'them': [3973], 'gave': [3974, 4058, 4074, 4717], 'FM-positive': [3979], 'peaks': [3980], 'might': [3981, 5896], 'fluorescent': [3987], 'reagent.': [3988], '6': [3994], 'cysteine': [3995, 4086, 4289], 'bonds': [4009, 4179, 5824], 'sites,': [4013, 4218], 'AspN.': [4021], 'double': [4023, 4416], 'obtain': [4028, 4172], 'glycopeptides.': [4033], 'map': [4036, 4197], 'demonstrated': [4040, 5079, 5819], 'significant': [4041, 5947], 'resistance': [4042], 'against': [4043], 'serine': [4045], 'Asp-N,': [4049], 'so': [4050], 'recovery': [4052], 'insufficient.': [4054], 'Nevertheless,': [4055], 'useful': [4059], 'information': [4060, 4174], 'elucidating': [4062], 'structure.': [4064, 5957], 'summarized': [4068], 'Table': [4070, 4390, 4473, 4518, 4552, 4779], 'Peptide': [4072, 4109, 4230, 4275, 4576], 'TD22.8': [4073, 4272], 'sequences,': [4076, 4281, 4350], 'DXK-(277–279)': [4077], 'DXGYNIPQT-(442–450),': [4079], 'where': [4080, 4285], 'X': [4081, 4286], 'according': [4088, 4635], '(Fig.3': [4094], 'Mass': [4096, 4119, 4238, 4325], 'supported': [4098], 'conclusion': [4100], 'linked': [4105], 'between': [4106, 4313, 4401, 5770], 'cysteines.': [4108, 5784], 'TD27.5a': [4110], 'similar,': [4113], 'C-terminally': [4115], 'extended': [4116], 'DXKEYNY-(277–283).': [4118], 'masses': [4131], '1376.5': [4133], '1946.1,': [4135], 'respectively.': [4136, 4726, 4755], 'From': [4137], 'assign': [4141], 'first': [4143, 4904], 'linkage': [4145, 4339], 'Cys278–Cys443.': [4147], 'Finally,': [4148, 4392], 'TD27.5b': [4150], 'peptides,': [4155, 4647], 'XK-(564–565)': [4156], 'TPEVTXVVVD-(499–508),': [4158], 'indicates': [4159, 4287], 'Cys504–Cys564': [4163], 'region.': [4167, 5092], 'Since': [4168, 5828], 'could': [4170, 5900], 'TD-digested': [4182], 'alone,': [4184], 'acidic': [4192], 'pepsin-generated': [4195, 4476], '6.': [4202], 'Sequence': [4203, 4768], 'revealed': [4208], 'key': [4210], 'determining': [4213], 'TableII.': [4229], 'P33.7': [4231], 'LKMDXKEY-(274–281)': [4235], 'DMEDXGYNIPQT-(439–450).': [4237], 'assignment': [4242], 'although': [4243], 'slightly': [4248], 'probably': [4253], 'due': [4254, 4321, 4333, 5762], 'oxidation': [4256], 'methionine': [4259], '7': [4262, 4374], 'already': [4268], 'assigned': [4269], 'above).': [4274], 'P37.7': [4276, 4316], 'P39': [4278, 4318], 'FSLQLXGAGFPLNQSEVL-(211–228)': [4282], 'AVSAXHVHDEF-(416–426),': [4284], 'residue.': [4290], 'permitted': [4293], 'Cys216–Cys420.': [4297], '223': [4301], 'because': [4308], 'theN-glycosylation.': [4310], 'heterogeneity.': [4324], 'successful': [4332], 'Cys330–Cys380': [4340], 'determined': [4342, 4383, 4579, 4827, 4866], 'P44.9,': [4347], 'LVXWQAGTTPWNIF-(328–341)': [4351], 'VATSQDDXYKF-(373–383).': [4353], '2915': [4358], 'P44.9': [4361], '2914.3': [4368], 'within': [4369], 'experimental': [4371, 4759], 'errors': [4372], 'Cys610–Cys668from': [4386], 'P34.6': [4388], 'II).': [4391], 'dimerized': [4394], 'P30.5': [4396], 'demonstrates': [4397], 'Cys469': [4402], 'Cys472': [4404], 'portion.Table': [4408], 'ISequences': [4409], 'BACE-IgGPeptideSequence': [4419, 4480, 4821], '(residue': [4420, 4481, 4822], 'no.)': [4421], 'aCysteine': [4422], 'analysis,': [4429, 4454], 'they': [4431, 4456, 5798], 'A).Observed': [4441], 'massCalculated': [4442, 4483], '(MH+)Disulfide': [4444, 4485], 'bondTD': [4445], '22.8DCK-(277–279)13751376.5Cys278–Cys443DCGYNIPQT-(442–450)TD27.5aDCKEYNY-(277–283)19421946.1Cys278–Cys443DCGYNIPQT-(442–450)TD27.5bTPEVTCVVVD-(499–508)11941197.4Cys504–Cys564CK-(564–565)a': [4446], 'Cysteine': [4447], 'Open': [4467, 4554, 4894], 'table': [4468, 4555, 4895], 'new': [4471, 4558, 4898], 'tab': [4472, 4559, 4899], 'IISequences': [4474], 'no.)Observed': [4482], 'bondsP30.5AVTDKTHTCPPCPAPEL(LG)-(461–477/461–479)': [4486], 'aTwo': [4487], 'similar': [4488, 4521, 5904], '(461–477': [4490, 4523], '461–479)': [4492, 4525], 'cross-linked.': [4494, 4527], 'did': [4496, 4511, 4529, 4545, 5806], 'cysteines': [4502, 4535], 'bonds.37303734.4Cys469–Cys472P33.7LKMDCKEY-(274–281)25522532.8Cys278–Cys443DMEDCGYNIPQT-(439–450)P34.6TCLVKGFYPSD-(609–619)39023903.1Cys610–Cys668SCSVMHEALHNHYTQKS-(667–683)P39.0FSLQLCGAGFPLNQSEVL-(211–228)bDue': [4506], 'N-glycosylation,': [4508, 4542], 'III).3139.5Cys216–Cys420AVSACHVHDEF-(416–426)P44.9LVCWQAGTTPWNIF-(328–341)29152914.3Cys330–Cys380VATSQDDCYKF-(373–383)a': [4519], 'bonds.b': [4539], 'Due': [4540, 4781], 'III).': [4553], '7Mass': [4561], 'peptides.': [4565], 'spectrum': [4568, 4573], 'P33.7.': [4570], 'P44.9.': [4575], 'IV.': [4585], 'matrix.View': [4596], 'identification': [4612], 'attachments': [4615], '(TableIII).': [4616], 'Four': [4617], 'Asn354)': [4624], 'one': [4628, 5335, 5895], '(Asn540)': [4630], 'peptic': [4646], 'found': [4649], 'four': [4652], 'potential': [4653], 'occupied': [4660], 'moieties.': [4663], 'fact': [4665], 'separately': [4675], 'heterogeneities.': [4687], 'example,': [4689], 'FINGSN-(170–175)': [4692], 'Asn172': [4694], 'peaks,': [4699], 'P14.4,': [4700], 'P14.9,': [4701], 'P15.3,': [4703], 'respectively': [4704], '6).': [4706], 'Moreover,': [4707], 'peak,': [4714], 'P27,': [4716], 'units,': [4720, 4754, 4812], '3158.5,': [4721], '3320.7,': [4722], '3524.1,': [4723], '3686.2,': [4725], 'According': [4727, 5785], 'glycopeptide': [4733], 'P27': [4734], 'VSIPHGPNVTVRA-(146–158)': [4738], '(mass': [4739], '=': [4740], '1347.5)': [4741], 'andN-glycans': [4742], 'should': [4746], '1811.0,': [4748], '1973.2,': [4749], '2176.6,': [4750], '2338.7': [4752], 'even': [4757, 5099, 5252], 'considering': [4758], 'errors,': [4760], 'our': [4761, 4919], 'predict': [4764], 'structures.': [4767], 'spectral': [4771, 4801], 'analyses': [4772], 'listed': [4777], 'III.': [4780], 'complexity': [4784], 'problem,': [4787], 'determination': [4788], 'still': [4795, 5274, 5726], 'progress,': [4797], 'fragmentation': [4802], 'carbohydrates': [4807], 'hexose': [4811], 'structural': [4817, 5568, 6004], 'heterogeneity.Table': [4818], 'IIIGlycopeptides': [4819], 'no.)Sites': [4823], 'aN-Glycosylation': [4824], 'no': [4829, 4868, 5269, 5946], 'detection': [4830, 4869], 'PTH-Asn': [4832, 4871], 'cycle.': [4836, 4875], 'Boldface': [4837, 4876], 'letters': [4838, 4877], 'NX(S/T)': [4842, 4881], 'forN-glycosylation.': [4843, 4882], 'P39.0': [4846, 4885], 'cross-linking': [4850, 4889], 'bond.Observed': [4854], '(Calculated)P14.8FINGSN-(170–175)Asn172ND': [4856], '(651.7)P19.0EVTNQSF-(351–357)Asn3543511.9': [4857], '(824.8)3674.0P24.0YVDGVEVHNAKTKPREEQYNST-(521–542)Asn5404148.2': [4858], '(2565.7)4309.84471.6P27.0VSIPHGPNVTVRA-(146–158)Asn1533158.5': [4859], '(1347.5)3320.73524.13686.2P39.0FSLQLCGAGFPLNQSEVL-(211–228)Asn2234120': [4860], '(3139.5)AVSACHVHDEF': [4861], '(416–426)4552a': [4862], 'bond.': [4893], 'study': [4901, 5567], 'provides': [4902], 'characterization': [4905], 'level.': [4916], 'began': [4918], 'addressing': [4922], 'domain,': [4935], 'processed': [5084], 'lacking': [5089], 'Apparently,': [5093], 'quite': [5097, 5250, 5752], 'efficient,': [5098], 'overexpression': [5101, 5227, 5254], 'conditions,': [5102], 'reported': [5106, 5314], 'least': [5173], 'tested': [5178], 'here': [5179, 5940], 'appear': [5185], 'limit': [5187], 'activity.': [5189], 'over,': [5197], 'initial': [5204], 'know': [5214], 'point': [5217], 'whether': [5218, 5230], 'massive': [5220], 'loss': [5221], 'artifact': [5228], 'proportion': [5233], 'expression': [5242], 'conditions': [5243], 'Once': [5246], 'processed,': [5247], 'glycosylated.': [5262], 'findings': [5264], 'there': [5266], 'almost': [5268], 'fully': [5270], 'epitope': [5278], 'nature': [5299], 'currently': [5306, 5775], 'investigation,': [5308], 'autocatalytic': [5311], 'mechanism,': [5312], '(13.Davies': [5317], 'Annu.': [5319, 5926], 'Rev.': [5320, 5927], 'Chem.': [5323, 5930], '1990;': [5324, 5931], '19:': [5325, 5932], '189-215Crossref': [5326, 5933], '(573)': [5329, 5936], 'seems': [5332], 'unlikely,': [5333], 'considers': [5336], 'biochemistry': [5402], 'made': [5409], 'use': [5410], 'much': [5484, 5732], 'conveniently': [5486], 'maintains': [5500], 'appears': [5564], 'justified': [5565], 'features': [5569], 'Sequencing': [5576], 'confirms': [5579], 'start': [5582], 'identifies': [5712], 'Thr23': [5717], 'cleaved': [5723], 'off,': [5724], 'propeptide.': [5729], 'lower': [5733], 'amounts': [5734], 'BACE.': [5758], 'Whether': [5759], 'transport': [5765], 'kinetics': [5766], 'differences': [5769, 6005], 'known.': [5777], 'SH-labeling': [5788], 'contain': [5793], 'any': [5794], 'cysteines,': [5796], 'Within': [5803], 'detect': [5808], 'dimeric': [5809], 'caused': [5811], 'covalent': [5813], 'bonds,': [5815], 'instead': [5817], 'linkages.': [5827], 'clearly': [5831], 'expect': [5897], 'including': [5909], 'cathepsin': [5911], 'D': [5912], 'immunodeficiency': [5917], 'virus': [5918], '13.Davies': [5924], 'members': [5953], 'Fig.8,': [5961], 'phytepsin,': [5963], 'plant': [5965], '(14.Kervinen': [5968], 'Tobin': [5970], 'G.J.': [5971], 'Costa': [5972], 'Waugh': [5974], 'D.S.': [5975], 'Wlodawer': [5976], 'Zdanov': [5978], 'EMBO': [5980], '18:': [5983], '3947-3955Crossref': [5984], '(137)': [5987], 'partial': [5991], 'similarity': [5992], 'big': [5997], 'loops': [5998], 'domain.': [6002], 'Obviously,': [6013], 'discussion': [6016], 'function-relationship': [6020], 'will': [6023], 'require': [6024], 'x-ray': [6025], 'crystallographic': [6026], 'studies.': [6027], 'Understanding': [6028], 'target': [6031], 'turn': [6043], 'out': [6044], 'crucial': [6047], 'drug': [6049], 'development.': [6050]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2154375856', 'counts_by_year': [{'year': 2024, 'cited_by_count': 2}, {'year': 2023, 'cited_by_count': 4}, {'year': 2022, 'cited_by_count': 4}, {'year': 2021, 'cited_by_count': 9}, {'year': 2020, 'cited_by_count': 10}, {'year': 2019, 'cited_by_count': 2}, {'year': 2018, 'cited_by_count': 5}, {'year': 2017, 'cited_by_count': 2}, {'year': 2016, 'cited_by_count': 8}, {'year': 2015, 'cited_by_count': 1}, {'year': 2014, 'cited_by_count': 6}, {'year': 2013, 'cited_by_count': 12}, {'year': 2012, 'cited_by_count': 9}], 'updated_date': '2024-12-10T04:44:36.163030', 'created_date': '2016-06-24'}