Title: Combining Mass Spectrometry and Peptide Arrays to Profile the Specificities of Histone Deacetylases
Abstract: ChemBioChemVolume 10, Issue 13 p. 2159-2161 Communication Combining Mass Spectrometry and Peptide Arrays to Profile the Specificities of Histone Deacetylases Zachary A. Gurard-Levin, Zachary A. Gurard-Levin Department of Chemistry and Howard Hughes Medical Institute, The University of Chicago, 929 East 57th Street, Chicago, IL 60521 (USA), Fax: (+1) 773-702-1677Search for more papers by this authorJoohoon Kim Prof., Joohoon Kim Prof. Present address: Department of Chemistry, Kyung Hee University, Hoegidong Dongdaemungu Seoul, 130-701 (Korea)Search for more papers by this authorMilan Mrksich Prof., Milan Mrksich Prof. [email protected] Department of Chemistry and Howard Hughes Medical Institute, The University of Chicago, 929 East 57th Street, Chicago, IL 60521 (USA), Fax: (+1) 773-702-1677Search for more papers by this author Zachary A. Gurard-Levin, Zachary A. Gurard-Levin Department of Chemistry and Howard Hughes Medical Institute, The University of Chicago, 929 East 57th Street, Chicago, IL 60521 (USA), Fax: (+1) 773-702-1677Search for more papers by this authorJoohoon Kim Prof., Joohoon Kim Prof. Present address: Department of Chemistry, Kyung Hee University, Hoegidong Dongdaemungu Seoul, 130-701 (Korea)Search for more papers by this authorMilan Mrksich Prof., Milan Mrksich Prof. [email protected] Department of Chemistry and Howard Hughes Medical Institute, The University of Chicago, 929 East 57th Street, Chicago, IL 60521 (USA), Fax: (+1) 773-702-1677Search for more papers by this author First published: 27 August 2009 https://doi.org/10.1002/cbic.200900417Citations: 56Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Graphical Abstract In profile: We describe the use of peptide arrays to probe the substrate specificities of several histone deacetylase (HDAC) enzymes. The combination with SAMDI-MS avoids the need for labels, which we demonstrate can perturb enzyme activity and lead to altered enzyme specificity. Supporting Information Detailed facts of importance to specialist readers are published as "Supporting Information". Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Filename Description cbic_200900417_sm_miscellaneous_information.pdf107.6 KB miscellaneous_information Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. References 1R. Frank, Tetrahedron 1992, 48, 9217. 2U. Reineke, R. Volkmer-Engert, J. Schneider-Mergener, Curr. Opin. Biotechnol. 2001, 12, 59. 3M. A. Cooper, Anal. Bioanal. Chem. 2003, 377, 834. 4T. P. Roddy, C. R. Horvath, S. J. Stout, K. L. Kenney, P. I. Ho, J. H. Zhang, C. Vickers, V. Kaushik, B. Hubbard, Y. K. Wang, Anal. Chem. 2007, 79, 8207. 5M. Mrksich, ACS Nano 2008, 2, 7. 6A. L. Garske, J. M. Denu, Biochemistry 2006, 45, 94. 7K. Hoffmann, G. Brosch, P. Loidl, M. Jung, Nucleic Acids Res. 1999, 27, 2057. 8K. T. Howitz, K. J. Bitterman, H. Y. Cohen, D. W. Lamming, S. Lavu, J. G. Wood, R. E. Zipkin, P. Chung, A. Kisielewski, L. L. Zhang, B. Scherer, D. A. Sinclair, Nature 2003, 425, 191. 9M. Kaeberlein, T. McDonagh, B. Heltweg, J. Hixon, E. A. Westman, S. D. Caldwell, A. Napper, R. Curtis, P. S. DiStefano, S. Fields, A. Bedalov, B. K. Kennedy, J. Biol. Chem. 2005, 280, 17038. 10M. T. Borra, B. C. Smith, J. M. Denu, J. Biol. Chem. 2005, 280, 17187. 11K. H. Zhao, X. M. Chai, R. Marmorstein, Structure 2003, 11, 1403. 12Z. A. Gurard-Levin, M. Mrksich, Biochemistry 2008, 47, 6242. 13B. T. Houseman, E. S. Gawalt, M. Mrksich, Langmuir 2003, 19, 1522. 14D. H. Min, W. S. Yeo, M. Mrksich, Anal. Chem. 2004, 76, 3923. 15M. G. Guenther, O. Barak, M. A. Lazar, Mol. Cell. Biol. 2001, 21, 6091. 16D. Riester, C. Hildmann, S. Grunewald, T. Beckers, A. Schwienhorst, Biochem. Biophys. Res. Commun. 2007, 357, 439. 17X. J. Yang, E. Seto, Nat. Rev. Mol. Cell Biol. 2008, 9, 206. 18D. F. Wang, P. Helquist, N. L. Wiech, O. Wiest, J. Med. Chem. 2005, 48, 6936. Citing Literature Volume10, Issue13September 4, 2009Pages 2159-2161 ReferencesRelatedInformation