Title: Heme oxygenase-1 expression protects melanocytes from stress-induced cell death: implications for vitiligo
Abstract: Experimental DermatologyVolume 20, Issue 6 p. 496-501 Original Article Heme oxygenase-1 expression protects melanocytes from stress-induced cell death: implications for vitiligo Yasser E. Elassiuty, Yasser E. Elassiuty Departments of Pathology, Microbiology and Immunology/Oncology Institute, Loyola University Chicago, IL, USA Department of Dermatology, Beni Suef University, Beni Suef, EgyptSearch for more papers by this authorJared Klarquist, Jared Klarquist Departments of Pathology, Microbiology and Immunology/Oncology Institute, Loyola University Chicago, IL, USASearch for more papers by this authorJodi Speiser, Jodi Speiser Departments of Pathology, Microbiology and Immunology/Oncology Institute, Loyola University Chicago, IL, USASearch for more papers by this authorRanda M. Yousef, Randa M. Yousef Department of Dermatology, Cairo University, Cairo, EgyptSearch for more papers by this authorAbdelaziz A. EL Refaee, Abdelaziz A. EL Refaee Department of Dermatology, Beni Suef University, Beni Suef, EgyptSearch for more papers by this authorNahla S. Hunter, Nahla S. Hunter Department of Dermatology, Cairo University, Cairo, EgyptSearch for more papers by this authorOlfat G. Shaker, Olfat G. Shaker Department of Biochemistry, Cairo University, Cairo, EgyptSearch for more papers by this authorMohan Gundeti, Mohan Gundeti Departments of Surgery and Pediatrics, University of Chicago, IL, USASearch for more papers by this authorLudmila Nieuweboer-Krobotova, Ludmila Nieuweboer-Krobotova The Netherlands Institute for Pigmentary disorders, Department of Dermatology, Academic Medical Center, University of Amsterdam, Amsterdam, the NetherlandsSearch for more papers by this authorI. Caroline Le Poole, I. Caroline Le Poole Departments of Pathology, Microbiology and Immunology/Oncology Institute, Loyola University Chicago, IL, USASearch for more papers by this author Yasser E. Elassiuty, Yasser E. Elassiuty Departments of Pathology, Microbiology and Immunology/Oncology Institute, Loyola University Chicago, IL, USA Department of Dermatology, Beni Suef University, Beni Suef, EgyptSearch for more papers by this authorJared Klarquist, Jared Klarquist Departments of Pathology, Microbiology and Immunology/Oncology Institute, Loyola University Chicago, IL, USASearch for more papers by this authorJodi Speiser, Jodi Speiser Departments of Pathology, Microbiology and Immunology/Oncology Institute, Loyola University Chicago, IL, USASearch for more papers by this authorRanda M. Yousef, Randa M. Yousef Department of Dermatology, Cairo University, Cairo, EgyptSearch for more papers by this authorAbdelaziz A. EL Refaee, Abdelaziz A. EL Refaee Department of Dermatology, Beni Suef University, Beni Suef, EgyptSearch for more papers by this authorNahla S. Hunter, Nahla S. Hunter Department of Dermatology, Cairo University, Cairo, EgyptSearch for more papers by this authorOlfat G. Shaker, Olfat G. Shaker Department of Biochemistry, Cairo University, Cairo, EgyptSearch for more papers by this authorMohan Gundeti, Mohan Gundeti Departments of Surgery and Pediatrics, University of Chicago, IL, USASearch for more papers by this authorLudmila Nieuweboer-Krobotova, Ludmila Nieuweboer-Krobotova The Netherlands Institute for Pigmentary disorders, Department of Dermatology, Academic Medical Center, University of Amsterdam, Amsterdam, the NetherlandsSearch for more papers by this authorI. Caroline Le Poole, I. Caroline Le Poole Departments of Pathology, Microbiology and Immunology/Oncology Institute, Loyola University Chicago, IL, USASearch for more papers by this author First published: 22 March 2011 https://doi.org/10.1111/j.1600-0625.2010.01232.xCitations: 15 I. Caroline Le Poole, PhD, Associate Professor of Pathology, Microbiology and Immunology, Loyola University Medical Center, Bldg 112, Rm 203, 2160 South First Avenue, Maywood, IL 60153 USA, Tel.: 1-708-327-2032, Fax: 1-708-327-3238, e-mail: [email protected] Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Abstract Abstract: To study protection of melanocytes from stress-induced cell death by heme oxygenases during depigmentation and repigmentation in vitiligo, expression of isoforms 1 and 2 was studied in cultured control and patient melanocytes and normal skin explants exposed to UV or bleaching agent 4-TBP. Similarly, expression of heme oxygenases was followed in skin from vitiligo patients before and after PUVA treatment. Single and double immunostainings were used in combination with light and confocal microscopic analysis and Western blotting. Melanocyte expression of heme oxygenase 1 is upregulated, whereas heme oxygenase 2 is reduced in response to UV and 4-TBP. Upregulation of inducible heme oxygenase 1 was also observed in UV-treated explant cultures, in skin of successfully PUVA-treated patients and in melanocytes cultured from vitiligo non-lesional skin. Heme oxygenase encoding genes were subsequently cloned to study consequences of either gene product on cell viability, demonstrating that HO-1 but not HO-2 overexpression offers protection from stress-induced cell death in MTT assays. HO-1 expression by melanocytes may contribute to beneficial effects of UV treatment for vitiligo patients. References 1 Arican O, Kurutas E B. Acta Dermatovenerol Alp Panonica Adriat 2008: 17: 12– 16. 2 Taïeb A, Picardo M, VETF Members. Pigment Cell Res 2007: 20: 27– 35. 3 Jin Y, Birlea S A, Fain P R et al. N Engl J Med 2010: 362: 1686– 1697. 4 Molina Garrido M J, Enríquez R, Mora Rufete A et al. Am J Med Sci 2007: 333: 178– 180. 5 Garbelli S, Mantovani S, Palermo B et al. Pigment Cell Res 2005: 18: 234– 242. 6 Song X, Xu A, Pan W et al. Int J Mol Med 2008: 22: 9– 16. 7 Westerhof W, d'Ischia M. Pigment Cell Res 2007: 5: 345– 359. 8 Schallreuter K U, Bahadoran P, Picardo M et al. Exp Dermatol 2008: 17: 139– 140. 9 Shalbaf M, Gibbons N C, Wood J M et al. 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